www.sciencetranslationalmedicine.org/cgi/content/full/3/68/68ra10/DC1 Supplementary Materials for Human CD3 Transgenic Mice: Preclinical Testing of Antibodies Promoting Immune Tolerance Chantal Kuhn, Sylvaine You, Fabrice Valette, Geoff Hale, Peter van Endert, Jean-François Bach,* Herman Waldmann, Lucienne Chatenoud *To whom correspondence should be addressed. E-mail: [email protected]Published 2 February 2011, Sci. Transl. Med. 3, 68ra10 (2011) DOI: 10.1126/scitranslmed.3001830 The PDF file includes: Materials and Methods Fig. S1. Antigenic modulation of CD3-TCR induced in vitro by anti-mouse or anti-human CD3 antibodies. Fig. S2. Proliferative response of splenocytes from NOD and NOD-huCD3ε mice to mitogenic anti-mouse CD3 antibody. Fig. S3. Induction of T cell activation as assessed by CD69 staining after in vivo CD3 antibody administration. Fig. S4. Insulitis in untreated and CD3 antibody–treated NOD-huCD3ε mice. Fig. S5. Dose-dependent inhibition of CD4 + CD25 - T cell proliferation by CD4 + CD25 + regulatory T cells. Table S1. In vitro conversion of CD4 + CD25 - peripheral T cells into CD4 + CD25 + FoxP3 + T cells in the presence of CD3 antibodies and TGF-β. Table S2. Induction of T cell activation as assessed by CD25 staining after in vivo CD3 antibody administration.
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Supplementary Materials for€¦ · 1/31/2011 · Results are expressed ... SUPPLEMENTARY TABLE 1 n vitro conversion of CD4. CD25- peripheral T cells . SUPPLEMENTARY TABLE 2. Induction
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Human CD3 Transgenic Mice: Preclinical Testing of Antibodies Promoting Immune Tolerance
Chantal Kuhn, Sylvaine You, Fabrice Valette, Geoff Hale, Peter van Endert,
Jean-François Bach,* Herman Waldmann, Lucienne Chatenoud
*To whom correspondence should be addressed. E-mail: [email protected]
Published 2 February 2011, Sci. Transl. Med. 3, 68ra10 (2011) DOI: 10.1126/scitranslmed.3001830
The PDF file includes:
Materials and Methods Fig. S1. Antigenic modulation of CD3-TCR induced in vitro by anti-mouse or anti-human CD3 antibodies. Fig. S2. Proliferative response of splenocytes from NOD and NOD-huCD3ε mice to mitogenic anti-mouse CD3 antibody. Fig. S3. Induction of T cell activation as assessed by CD69 staining after in vivo CD3 antibody administration. Fig. S4. Insulitis in untreated and CD3 antibody–treated NOD-huCD3ε mice. Fig. S5. Dose-dependent inhibition of CD4+CD25− T cell proliferation by CD4+CD25+ regulatory T cells. Table S1. In vitro conversion of CD4+CD25− peripheral T cells into CD4+CD25+FoxP3+ T cells in the presence of CD3 antibodies and TGF-β. Table S2. Induction of T cell activation as assessed by CD25 staining after in vivo CD3 antibody administration.
SUPPLEMENTARY MATERIAL (C. Kuhn et al.)
MATERIALS AND METHODS
Figure S1
Splenic T cells from NOD or NOD-huCD3ε mice were isolated by negative selection using