www.sciencemag.org/content/361/6408/1246/suppl/DC1 Supplementary Material for Ancient steroids establish the Ediacaran fossil Dickinsonia as one of the earliest animals Ilya Bobrovskiy*, Janet M. Hope, Andrey Ivantsov, Benjamin J. Nettersheim, Christian Hallmann, Jochen J. Brocks* *Corresponding author. Email: [email protected] (I.B.); [email protected] (J.J.B.) Published 20 September 2018, Science 361, 1246 (2017) DOI: 10.1126/science.aat7228 This PDF file includes: Materials and Methods Supplementary Text Figs. S1 to S6 Tables S1 to S4 References
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Supplementary Material for · 9/19/2018 · The GC was equipped with a 60 m DB-5 MS capillary column (0.25 mm i.d., 0.25 m film thickness; Agilent JW Scientific, Agilent Technologies,
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Table S1. Estimation of the sterol composition of Dickinsonia. 1 Concentration of C27, C28 and C29 monoaromatic steroid (MAS) homologs (sum of isomers I
and V) relative to total hopanes (sum of C27–C35 hopanes) in Dickinsonia extracts; hopanes:
17α(H)21β(H), βα = 17β(H)21α(H); y indicates carbon number of monoaromatic steroids; 25β/5α = (βαα20R+ααα20S)/ααα20R; 3Variance = ∑(𝑥 − �̅�)2 (𝑛 − 1)⁄ , where 𝑥 is Cy/H
value, �̅� is a mean Cy/H value between the samples and 𝑛 is the number of samples (𝑛 = 3); 4Cy% = 100%*(Cy variance)/(sum of variances for each of three steroids), y indicates carbon
number of monoaromatic steroids; 5numbers in parentheses are standard deviation values.
Table S2. Estimation of sterol composition of Andiva. 15β/5α = (βαα20R+ααα20S)/ααα20R; 2Variance = ∑(𝑥 − �̅�)2 (𝑛 − 1)⁄ , where 𝑥 is Cy/H
value, �̅� is a mean 5β/5α value for each sterane between the samples and 𝑛 is the number of
samples (𝑛 = 3); 3Cy% = 100%*(Cy variance)/(sum of variances for each of three steroids); 4numbers in parentheses are standard deviation values; 5conservative minimum estimate of
relative cholesteroid content (C27%) of Andiva (see Supplementary Text).
Acantharea sp. Acantharea Indian Ocean near Eden 51.98 39.35 8.67
Gromia sp. Gromiidae Southern Ocean
(McMurdo Sound) 11.77 27.88 60.35
A. latticolaris1 Foraminifera Literature (31) 10.31 26.93 62.76
Table S4. Distribution of C27-C29 sterols in extant Rhizaria (Retaria and Gromiidae) 1Values for A. latticolaris were determined by integrating peak areas of the functionalized
sterols in Fig.1a of Grabenstatter et al. (31).
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