10.1071/RD14310_AC © CSIRO 2015 Supplementary Material: Reproduction, Fertility and Development, 2015, 27(4), 621–637. Supplementary Material Ambient ionisation mass spectrometry for lipid profiling and structural analysis of oocytes, preimplantation embryos and stem cells Christina R. Ferreira A,F , Alan K. Jarmusch A , Valentina Pirro A , Clint M. Alfaro A , Andres F. González- Serrano B,E , Heiner Niemann B , Matthew B. Wheeler C , Rathnaweera A. C. Rabel C , Judy E. Hallett D , Rebecca Houser D , Annemarie Kaufman D and R. Graham Cooks A A Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University, 207 South Martin Jischke Drive, West Lafayette, IN 47907, USA. B Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Höltystr. 10, 31535 Neustadt, Germany. C Laboratory of Stem Cell Biology and Engineering, Department of Animal Sciences and Institute for Genomic Biology University, University of Illinois at Urbana-Champaign, 1207 W. Gregory, Urbana, IL 61801, USA. D Purdue Center for Cancer Research Transgenic Mouse Core Facility, Purdue University, 201 S. University Street, West Lafayette, IN 47907, USA. E Present address: Research and Development Department, IMV Technologies, ZI N° 1 Est, 61300 L’Aigle, France. F Corresponding author. Email: [email protected]
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
10.1071/RD14310_AC © CSIRO 2015 Supplementary Material: Reproduction, Fertility and Development, 2015, 27(4), 621–637.
Supplementary Material
Ambient ionisation mass spectrometry for lipid profiling and structural analysis of oocytes, preimplantation embryos and stem cells
Christina R. FerreiraA,F, Alan K. JarmuschA, Valentina PirroA, Clint M. AlfaroA, Andres F. González-
SerranoB,E, Heiner NiemannB, Matthew B. WheelerC, Rathnaweera A. C. RabelC, Judy E. HallettD,
Rebecca HouserD, Annemarie KaufmanD and R. Graham CooksA
ADepartment of Chemistry and Center for Analytical Instrumentation Development, Purdue
University, 207 South Martin Jischke Drive, West Lafayette, IN 47907, USA.
BInstitute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Höltystr. 10, 31535 Neustadt,
Germany.
CLaboratory of Stem Cell Biology and Engineering, Department of Animal Sciences and Institute for
Genomic Biology University, University of Illinois at Urbana-Champaign, 1207 W. Gregory, Urbana,
IL 61801, USA.
DPurdue Center for Cancer Research Transgenic Mouse Core Facility, Purdue University, 201 S.
University Street, West Lafayette, IN 47907, USA.
EPresent address: Research and Development Department, IMV Technologies, ZI N° 1 Est, 61300
L’Aigle, France.
FCorresponding author. Email: [email protected]
Fig. S1. Mass spectrometer capable of desorption electrospray ionization analysis of biological samples. Front view of the experimental set up. (1) Silica capillaries that direct the electrospray at the sample. (2) Source of nebulizing N2. (3) Solvent introduction line held at high voltage. (4) Extended capillary inlet to the mass spectrometer. (5) Computer controlled moving stage that manipulates sample position. (6) Computer for instrument control and data storage. Close up view of the DESI sprayer, sample, and extended capillary inlet.
Related Documents
