Supplementary Figure S1. 1 H- 15 N HSQC spectra of 15 N-labeled ZG16p titrated with Man-O-Thr (a), Man- O-peptide (b), Manα1-3Man (c) and Glc1-3Glc (d). Marked peaks are selected for the calculation of dissociation constants (Figure 4). 15 N 15 N 1 H 1 H Arg side chain (Hδ) G147 G33 S146 G147 G35 E29 S146 G33 S146 G147 G33 (a) Man-O-Thr (b) Mannose-O-peptide (c) Mana1-3Man (d) Glc1-3Glc 0 1 2 5 10 20 50 100 0 1 2 5 10 20 50 100 0 1 5 10 20 50 100 200 0 1 5 10 20 50 100 Molar ratio Ligand:prot ein Molar ratio Ligand:prot ein Molar ratio Ligand:prot ein Molar ratio Ligand:prot ein 1 H 1 H
Supplementary Figure S1.
Dec 31, 2015
Supplementary Figure S1. 1 H- 15 N HSQC spectra of 15 N-labeled ZG16p titrated with Man- O - Thr (a), Man- O -peptide (b), Manα1-3Man (c) and Glc 1-3Glc (d). Marked peaks are selected for the calculation of dissociation constants (Figure 4). (a) Man- O -Thr. (b) Mannose- O -peptide. - PowerPoint PPT Presentation
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Supplementary Figure S1.1H-15N HSQC spectra of 15N-labeled ZG16p titrated with Man-O-Thr (a), Man-O-peptide (b), Manα1-3Man (c) and Glc1-3Glc (d). Marked peaks are selected for the calculation of dissociation constants (Figure 4).
15N
15N
1H 1H
Arg side chain (Hδ)
G147
G33
S146
G147
G35
E29
S146
G33
S146
G147
G33
(a) Man-O-Thr (b) Mannose-O-peptide
(c) Mana1-3Man (d) Glcb1-3Glc
0125
102050
100
0125
102050
100
015
102050
100200
0
1
5
10
20
50
100
Molar ratioLigand:protei
n
Molar ratioLigand:protei
n
Molar ratioLigand:protei
n
Molar ratioLigand:protei
n
1H 1H
(a) Man-O-Ser (b) Man-O-Thr
(c) Mana1-3Man (d) Glcb1-3Glc
Supplementary Figure S2. The observed electron density of each ligand. Fo-Fc electron density maps of each ligand are shown in gray mesh, countered at 1s level ((a), (c) and (d)) or 0.5 s level (b). (a) Man-O-Ser/ZG16p, (b) Man-O-Thr/ZG16p, (c) Manα1-3Man/ZG16p and (d) Glc1-3Glc/ZG16p.
Asp151
Tyr104 Tyr104
Asp151
Tyr104
Asp151
Asp151
Tyr104
Ser148
Ser148
Ser148Ser
148
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