Structure, Volume 20 Supplemental Information Solution Structure of the ESCRT-I and -II Supercomplex: Implications for Membrane Budding and Scission Evzen Boura, Bartosz Różycki, Hoi Sung Chung, Dawn Z. Herrick, Bertram Canagarajah, David S. Cafiso, William A. Eaton, Gerhard Hummer, and James H. Hurley Inventory of Supplemental Information Movie S1. Animated model for sorting, budding, and scission. Associated with Figure 8. Fig. S1 SAXS analysis. Associated with Figure 1. Fig. S2 Simulation of the ESCRT-II complex. Associated with Figure 3. Fig. S3 Simulation of the ESCRT-I-II supercomplex. Associated with Figure 4. Fig. S4 Dependence of 2 on number of conformations used in fitting. Associated with Figure 5.
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Structure, Volume 20 Supplemental Information
Solution Structure of the ESCRT-I and -II
Supercomplex: Implications
for Membrane Budding and Scission
Evzen Boura, Bartosz Różycki, Hoi Sung Chung, Dawn Z. Herrick, Bertram Canagarajah, David S. Cafiso, William A. Eaton, Gerhard Hummer, and James H. Hurley Inventory of Supplemental Information
Movie S1. Animated model for sorting, budding, and scission. Associated with
Figure 8.
Fig. S1 SAXS analysis. Associated with Figure 1.
Fig. S2 Simulation of the ESCRT-II complex. Associated with Figure 3.
Fig. S3 Simulation of the ESCRT-I-II supercomplex. Associated with Figure 4.
Fig. S4 Dependence of 2 on number of conformations used in fitting. Associated
with Figure 5.
Supplementary Movie and Figure Legends
Movie S1. Animated model for sorting, budding, and scission. See the legend to Fig. 8
for detailed explanations of each event and the data supporting it.
Fig. S1 SAXS analysis. SAXS for ESCRT-I-II at two different concentrations shown
unscaled (A) and scaled (B). Associated with Figure 1.
Fig. S2 Simulation of the ESCRT-II complex. Simulation data before any refinement
(black) are compared to experimental data (red). Panels (A) to (C) show smFRET
efficiency histograms for labels 282 Vps36 - 34 Vps22 (A), 96 Vps36 - 34 Vps22 (B) and