1 2 Supplemental Figure S1. Southern-blot linkage analysis of the pollen semi-sterile (PSS) 3 phenotype (cap1 mutation) and Tos17 insertions. Ten or more bands showing the Tos17 4 transposition were detected in each lane. An approximately 3-kb band (arrow) was always detected 5 in plants with the PSS phenotype (m lanes) but never in wild-type plants (w lanes). The leftmost 6 lane contained lambda DNA/HindIII marker; numbers are sizes. 7
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Supplemental Figure S1. - Plant physiology 2 3 Supplemental Figure S1. Southern-blot linkage analysis of the pollen semi-sterile (PSS) 4 phenotype (cap1 mutation) and Tos17 insertions.
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Supplemental Figure S1. Southern-blot linkage analysis of the pollen semi-sterile (PSS) 3
phenotype (cap1 mutation) and Tos17 insertions. Ten or more bands showing the Tos17 4
transposition were detected in each lane. An approximately 3-kb band (arrow) was always detected 5
in plants with the PSS phenotype (m lanes) but never in wild-type plants (w lanes). The leftmost 6
lane contained lambda DNA/HindIII marker; numbers are sizes. 7
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Supplemental Figure S2. Genotyping of ND2104-line plants by PCR. The PCR mixture contained 4
three primers (CP-2278, CP-3401, and T17L in Figs. 2A and S2A and Table S1). (A) With 1 min 5
PCR extension, the fragments of wild-type and Tos17-insertion DNA were 762 (a) and 532 (b) bp, 6
respectively. (B) PCR products were used to genotype the ND2104 locus. w, wild-type (+/+); h, 7