SUPPLEMENT 2 This power point presentation reports analyses of RyR distribution sured by confocal microscopy in tangential sections of 6 SA node ce Summary of the analyses numbers are those assigned for 25 cells reported in Supplemental Figure 1 *Cell#, section All peaks Peaks with higher amplitudes (> Mean+2 x SD)** Number of peaks #peaks with over- micron neighbors Nearest neighbor distance, mm Mean±SD Number of peaks #peaks with over- micron neighbors Nearest neighbor distance, mm Mean±SD Cell#5,bottom 319 79 (24.8%) 0.839±0.2 25 20 20 (51.3%) 1.186±1.1 30 Cell#10,bottom 116 7 (6.0%) 0.710±0.2 23 31 18 (58.1%) 1.044±0.4 74 Cell#8,top 122 35 (28.7%) 0.871±0.2 73 18 11 (61.1%) 1.863±1.1 88 Cell#11,top &bottom combined 365 118 (32.4%) 0.888±0.3 00 74 43 (58.1%) 1.255±0.8 31 Cell#13,bottom 153 32 (20.9%) 0.775±0.2 76 47 27 (57.5%) 1.112±0.5 29 Cell#19,bottom 149 21 (14.1%) 0.719±0.2 89 39 20 (51.3%) 1.186±1.1 30 Average ± SEM (21.2±4.0 %) 0.800±0.0 3 (56.2±1.6 %) 1.274±0.1 21 D in finding peaks with higher amplitude (>mean+2 x SD) was calculated for orescence peaks given in the units of the SD of the total fluorescence signal
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SUPPLEMENT 2 This power point presentation reports analyses of RyR distribution
SUPPLEMENT 2 This power point presentation reports analyses of RyR distribution measured by confocal microscopy in tangential sections of 6 SA node cells. Summary of the analyses. * Cell numbers are those assigned for 25 cells reported in Supplemental Figure 1. - PowerPoint PPT Presentation
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SUPPLEMENT 2This power point presentation reports analyses of RyR distribution
measured by confocal microscopy in tangential sections of 6 SA node cells
Summary of the analyses
* Cell numbers are those assigned for 25 cells reported in Supplemental Figure 1
*Cell#, section All peaks Peaks with higher amplitudes (> Mean+2 x SD)**
** The SD in finding peaks with higher amplitude (>mean+2 x SD) was calculated for fluorescence peaks given in the units of the SD of the total fluorescence signal
original
cell#5_bottom
20 mm
5*
cell#5_bottomIntensity peaks of RyR labeling identified by “SD-slicing” computer algorithm (color dots).
-0.6 0 0.6 1.2 1.8 2.4 3.0 3.6 4 4.6 5.2 5.8
Peak amplitudes are coded by a rainbow-color scheme
Units are SD of total cell signalMean of total cell signal