biotech Ab presents Sunniest C18-HT 2μm biotech in Partnership with HPLC column
biotech Ab presents Sunniest C18-HT 2μm
biotech in Partnership with
HPLC column
Biotech SunnieSt c18-ht 2 μm
Reatures• Lowbackpressureandhighefficiencybypreciselyclassified
particle
• Highpressurepacking(10,000psi)usinghardsilicagelswithhighpressureresistant•leadslongcolumnlifewithoutanyvoid.
• UniquebondingtechniqueforSunniest(patentpending)
• Themostsuitableinnersurfaceofcolumnbyspecialgrinding
Conventional2μmsilicagelparticlewasclassifiedagain.20%volumewascutofffrombothsidesrespectively.Consequentlycolumnbackpressurereducedmorethan15%.Our2μmsilicagelparticleshowsahalfpressuretocomparewiththeothersub-2μmsilicagelparticle.
Itisdifficulttoend-caponsub2μmor2μmsilicagelparticleaswellas3μmor5μmsilicagelparticle.Mostsub2μmor2μmC18columnsshowsmallerplatenumberandhighertailingfactorthanSunniestC18-HT.SunniestC18-HT2μmshowsgoodpeakshapeforamitriptylineundernotonlymetahnol/phosphatebuffermobilephasebutalsoacetonitrile/phosphatebuffermobile.
Column: SunniestC18-HT,2μm50x2.1mmMobilephase: CH3OH/H2O,CH3CN/H2OFlowrate: 0.5mL/minTemperature: 40ºC
Column: SunniestC18-HT,2mm50x2.0mmMobilephase:A)CH3OH/20mMPhosphatebufferpH7.5=80/20B)CH3CN/20mMPhosphatebufferpH7.0=60/40Flowrate: 0.4mL/minPressure: A)19.5MPa,B)13.5MPaTemperature: 40ºCSample: 1=Uracil,2=Propranolol, 3=Nortriptyline,4=Amitriptyline,
Nortriptyline
Measured by Coulter Counter method Column pressure using methanol or acetonitrile and water
Sunniest technique to 2 μm particle!
narrow Particle DiStriBution anD low Back PreSSure
• an unique MoDification (Patent PenDing aS SunnieSt SerieS)
Retention time (min)
3 4
4
1
2
A Methanol
3
4
N(4) =7,529
TF(4) =1.19 4
1 2
B Acetonitrile Other C18
Other C18
N(4) =7,670
TF(4) =1.50 N(4) =8,068
TF(4) =1.13
N(4) =3,068
TF(4) =4.26
TF: USP tailing factor
1.0 2.0
Methanol
Acetonitrile
D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32
D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59
( )*'
Pres
sure(
MPa)
Concentration of organic solvent (%)
Narrow particle distribution
Conventional particle Classified particle
1.13 1.57
1.63 1.49
1.72 1.58
8,068 6,209
5,785 6,008 6,108
5,061
Comparison of tailing factor
Comparison of plate number
Retention time (min)
3 4
4
1
2
A Methanol
3
4
N(4) =7,529
TF(4) =1.19 4
1 2
B Acetonitrile Other C18
Other C18
N(4) =7,670
TF(4) =1.50 N(4) =8,068
TF(4) =1.13
N(4) =3,068
TF(4) =4.26
TF: USP tailing factor
1.0 2.0
Methanol
Acetonitrile
D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32
D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59
( )*'
Pres
sure(
MPa)
Concentration of organic solvent (%)
Narrow particle distribution
Conventional particle Classified particle
1.13 1.57
1.63 1.49
1.72 1.58
8,068 6,209
5,785 6,008 6,108
5,061
Comparison of tailing factor
Comparison of plate number
Retention time (min)
3 4
4
1
2
A Methanol
3
4
N(4) =7,529
TF(4) =1.19 4
1 2
B Acetonitrile Other C18
Other C18
N(4) =7,670
TF(4) =1.50 N(4) =8,068
TF(4) =1.13
N(4) =3,068
TF(4) =4.26
TF: USP tailing factor
1.0 2.0
Methanol
Acetonitrile
D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32
D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59
( )*'
Pres
sure(
MPa)
Concentration of organic solvent (%)
Narrow particle distribution
Conventional particle Classified particle
1.13 1.57
1.63 1.49
1.72 1.58
8,068 6,209
5,785 6,008 6,108
5,061
Comparison of tailing factor
Comparison of plate number
Retention time (min)
3 4
4
1
2
A Methanol
3
4
N(4) =7,529
TF(4) =1.19 4
1 2
B Acetonitrile Other C18
Other C18
N(4) =7,670
TF(4) =1.50 N(4) =8,068
TF(4) =1.13
N(4) =3,068
TF(4) =4.26
TF: USP tailing factor
1.0 2.0
Methanol
Acetonitrile
D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32
D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59
( )*'
Pres
sure(
MPa)
Concentration of organic solvent (%)
Narrow particle distribution
Conventional particle Classified particle
1.13 1.57
1.63 1.49
1.72 1.58
8,068 6,209
5,785 6,008 6,108
5,061
Comparison of tailing factor
Comparison of plate number
Retention time (min)
3 4
4
1
2
A Methanol
3
4
N(4) =7,529
TF(4) =1.19 4
1 2
B Acetonitrile Other C18
Other C18
N(4) =7,670
TF(4) =1.50 N(4) =8,068
TF(4) =1.13
N(4) =3,068
TF(4) =4.26
TF: USP tailing factor
1.0 2.0
Methanol
Acetonitrile
D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32
D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59
( )*'
Pres
sure(
MPa)
Concentration of organic solvent (%)
Narrow particle distribution
Conventional particle Classified particle
1.13 1.57
1.63 1.49
1.72 1.58
8,068 6,209
5,785 6,008 6,108
5,061
Comparison of tailing factor
Comparison of plate number
Retention time (min)
3 4
4
1
2
A Methanol
3
4
N(4) =7,529
TF(4) =1.19 4
1 2
B Acetonitrile Other C18
Other C18
N(4) =7,670
TF(4) =1.50 N(4) =8,068
TF(4) =1.13
N(4) =3,068
TF(4) =4.26
TF: USP tailing factor
1.0 2.0
Methanol
Acetonitrile
D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32
D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59
( )*'
Pres
sure(
MPa)
Concentration of organic solvent (%)
Narrow particle distribution
Conventional particle Classified particle
1.13 1.57
1.63 1.49
1.72 1.58
8,068 6,209
5,785 6,008 6,108
5,061
Comparison of tailing factor
Comparison of plate number
Retention time (min)
3 4
4
1
2
A Methanol
3
4
N(4) =7,529
TF(4) =1.19 4
1 2
B Acetonitrile Other C18
Other C18
N(4) =7,670
TF(4) =1.50 N(4) =8,068
TF(4) =1.13
N(4) =3,068
TF(4) =4.26
TF: USP tailing factor
1.0 2.0
Methanol
Acetonitrile
D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32
D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59
( )*'
Pres
sure(
MPa)
Concentration of organic solvent (%)
Narrow particle distribution
Conventional particle Classified particle
1.13 1.57
1.63 1.49
1.72 1.58
8,068 6,209
5,785 6,008 6,108
5,061
Comparison of tailing factor
Comparison of plate numberUracil Propranolol
Amitriptyline
Biotech SunnieSt c18-ht 2 μm
2μmparticleallowstoreduceretentiontimebecausehighefficiencyiskeptunderhighflowrateconditions.Asshowntheabovechromato-grams,analyticaltimereduced1/8withoutsac-rificesofseparationbyusing2μm,50x2.1μmcolumninsteadof5μm150x4.6μmcolumn.
Itisveryimportantfor2μmparticletohaveacapacitytoresistpressurebecauseofhighcolumnbackpressure.Thelargeraporevolumeofsilicagel,theweakeracapacitytoresistpressure.Thesilicagelwith0.85ml/gofporevolumeisusedforSunni-estC18-HT,2μm,sothatithaveahighcapacitytoresistpressureandahighoperatingpressure.
SiliCa gel SuppoRt C18
packings particle size pore diameterSpecific sur-
face areaCarbon content
Bonded phase
Maximum operating pressure available pH range
SunniestC18-HT 2.0μm(Coultercounter)
10nm 340m2/g 16% C18 70MPaor10,000psi
1.5-10
Column: SunniestC18-HT,2mm50x2.0mmMobilephase:A)CH3OH/20mMPhosphatebufferpH7.5=80/20B)CH3CN/20mMPhosphatebufferpH7.0=60/40Flowrate: 0.4mL/minPressure: A)19.5MPa,B)13.5MPaTemperature: 40ºCSample: 1=Uracil,2=Propranolol, 3=Nortriptyline,4=Amitriptyline,
Mobilephase: CH3CN/0.1%Formicacid=20/80Flowrate: 1.0mL/minfor150x4.6mm 0.6mL/minfor50x2.1mmTemperature: 40ºCDetection: UV@230nmSample: 1=Acetaminophen,2=Antipyrine, 3=Aspirin,4=Ethenzamide
Mobilephase: CH3CN/H2O=60/40Flowrate: 0.6mL/minfor2.1x30mmand2.1x50mm 1.0mL/minfor3.0x50mm 0.4mL/minfor2.1x75mmand2.1x100mmTemperature: 40ºCDetection: UV@250nmSample: 1=Uracil,2=Toluene, 3=Acenaphthene,4=Butylbenzene
SeParation of analgeSicS
characteriSticS of SunnieSt c18-ht 2 μm
coMPariSon of Plate no.
Sunniest C18 5 m, 150 x 4.6 mm
1
2 3
12
4
Retention time (min) 10
Sunniest C18-HT 2 m, 50 x 2.1 mm
N(4)=175,000 plate/m
N(4)=80,000 plate/m
High throughput
30 x 2.1 mm N(3)= 5,375
50 x 2.1 mm N(3)= 9,294
50 x 3.0 mm N(3)= 9,303
75 x 2.1 mm N(3)= 14,021
100 x 2.1 mm N(3)= 19,802
Retention time (min)
4
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
4
4
4
4
4321Retention time (min)
0
Sunniest C18 5 m, 150 x 4.6 mm
1
2 3
12
4
Retention time (min) 10
Sunniest C18-HT 2 m, 50 x 2.1 mm
N(4)=175,000 plate/m
N(4)=80,000 plate/m
High throughput
30 x 2.1 mm N(3)= 5,375
50 x 2.1 mm N(3)= 9,294
50 x 3.0 mm N(3)= 9,303
75 x 2.1 mm N(3)= 14,021
100 x 2.1 mm N(3)= 19,802
Retention time (min)
4
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
4
4
4
4
4321Retention time (min)
0
Ethenzamide
Sunniest C18 5 m, 150 x 4.6 mm
1
2 3
12
4
Retention time (min) 10
Sunniest C18-HT 2 m, 50 x 2.1 mm
N(4)=175,000 plate/m
N(4)=80,000 plate/m
High throughput
30 x 2.1 mm N(3)= 5,375
50 x 2.1 mm N(3)= 9,294
50 x 3.0 mm N(3)= 9,303
75 x 2.1 mm N(3)= 14,021
100 x 2.1 mm N(3)= 19,802
Retention time (min)
4
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
4
4
4
4
4321Retention time (min)
0
Sunniest C18 5 m, 150 x 4.6 mm
1
2 3
12
4
Retention time (min) 10
Sunniest C18-HT 2 m, 50 x 2.1 mm
N(4)=175,000 plate/m
N(4)=80,000 plate/m
High throughput
30 x 2.1 mm N(3)= 5,375
50 x 2.1 mm N(3)= 9,294
50 x 3.0 mm N(3)= 9,303
75 x 2.1 mm N(3)= 14,021
100 x 2.1 mm N(3)= 19,802
Retention time (min)
4
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
4
4
4
4
4321Retention time (min)
0
Acetaminophen
Sunniest C18 5 m, 150 x 4.6 mm
1
2 3
12
4
Retention time (min) 10
Sunniest C18-HT 2 m, 50 x 2.1 mm
N(4)=175,000 plate/m
N(4)=80,000 plate/m
High throughput
30 x 2.1 mm N(3)= 5,375
50 x 2.1 mm N(3)= 9,294
50 x 3.0 mm N(3)= 9,303
75 x 2.1 mm N(3)= 14,021
100 x 2.1 mm N(3)= 19,802
Retention time (min)
4
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
4
4
4
4
4321Retention time (min)
0
Antipyrine
Sunniest C18 5 m, 150 x 4.6 mm
1
2 3
12
4
Retention time (min) 10
Sunniest C18-HT 2 m, 50 x 2.1 mm
N(4)=175,000 plate/m
N(4)=80,000 plate/m
High throughput
30 x 2.1 mm N(3)= 5,375
50 x 2.1 mm N(3)= 9,294
50 x 3.0 mm N(3)= 9,303
75 x 2.1 mm N(3)= 14,021
100 x 2.1 mm N(3)= 19,802
Retention time (min)
4
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
4
4
4
4
4321Retention time (min)
0
Aspirin
g company Y company S1 company S2 company W company ChromaNik technologies
Ra 0.34μm 0.32μm 0.37μm 0.03μm 0.20μm 0.01μm
Rz 1.88μm 1.62μm 1.91μm 0.19μm 0.90μm 0.10μm
biotech Ab
P.O. BOx 133
SE-439 23 OnSala
SwEdEn
tEl +46 (0)300-56 91 80
fax +46 (0)300-56 91 81
www.biotech.se
narrow Particle DiStriBution anD low Back PreSSure
orDering inforMation of SunnieSt c18-ht 2 μm
Center line Ra R
Schematic diagram of surface roughness
parameter of surface roughnessRa:Averageroughnessfromcenterline
Rz:Roughnesscalculatedfrom10pointsaverage(5pointsofmaximumand5pointsofminimum)
Itisconsideredthatsurfaceroughnessaffectscol-umnperformance.SurfaceasperityofChromaNikTechnologiescolumnis1/30to1/20tocomparewiththatofGLSciences,YMC,ShimadzuandWaters
columns.ChromaNIkTechnologiesprovidesacolumnwithverysmoothsurfacewhichisthemostsuitablefor2μmparticlepacking.
InnersurfaceofcolumnofA,B,Ccompanies InnersurfaceofChromaNikTechnologies
2μm
particle
2μm
particle
length (mm) 30 50 75 100
inner diameter (mm) Cat. No. Cat. No. Cat. No. Cat. No.
2.1 EB1931 EB1941 EB1951 EB1961
3.0 EB1331 EB1341 EB1351 EB1361