Sudeep Perumbakkam Sudeep Perumbakkam Oregon State University Oregon State University
Dec 22, 2015
What is remediation? What is remediation? Hazardous waste is everywhere.Hazardous waste is everywhere. It comes from paints, motor oil, hair It comes from paints, motor oil, hair
spray, household cleaners, spray, household cleaners, automotive chemicals, and all kinds automotive chemicals, and all kinds of toxic medical, industrial and of toxic medical, industrial and military products. military products.
BIOREMEDIATIONBIOREMEDIATION
Bioremediation is a complete, safe and natural process of cleaning up organic contaminants through the use of microbes.
Pilot Greenhouse Study Pilot Greenhouse Study Fescue in sand, loam and clayFescue in sand, loam and clay
Plants are treated with a solution of 14C-TNT
Treated plants were removed from the soil 7 days after dosing.
Sand Loam Clay
Sand
Loam
Clay
Cuttings of foliage from grass grown in Cuttings of foliage from grass grown in soil treated with soil treated with 1414C-TNT C-TNT
Sand Loam Clay Sand Loam Clay Sand Loam Clay
11 days after
treatment with 14C-TNT
27 days after
treatment with 14C-TNT
47 days after
treatment with 14C-TNT
Cuttings of foliage from grass grown in Cuttings of foliage from grass grown in soil treated with soil treated with 1414C-TNT (continued)C-TNT (continued)
Sand Loam Clay Sand Loam Clay
69 days after
treatment with 14C-TNT
89 days after
treatment with 14C-TNT
Approximate excretion of Approximate excretion of 1414C-TNT C-TNT in sheepin sheep
LiverKidneyMuscleFat
Body tissues: 17-19%
Urine: 6-8%
Feces: 75%
LiverKidneyMuscleFat
Body tissues: 17-19%
Urine: 6-8%
Feces: 75%
Anaerobic transformation of 2,4,6-TNT by bovine Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbesruminal microbes
T.J. Fleischmann et al. (2004) T.J. Fleischmann et al. (2004) Biochemical and Biophysical Research Biochemical and Biophysical Research CommunicationsCommunications 314:957–963 314:957–963
Proposed Transformation Pathway of 2,4,6-Trinitrotoluene by Rumen Microbes Under Anaerobic Conditions