Writer: 이이이 이이이 Advisor: Prof. 이이이 이이이이이이이
Nov 19, 2014
Writer: 이흐완 히다얏
Advisor: Prof. 김석영
생체재료실험실
Contents
Introduction
Research Background
Research Objectives
Research Methodology
Research Progress
Research Plans
Introducti
on
Nowadays
Initially
Research & Development
Used to improve a patient’s masticator function
Patients desired also the aesthetics, short treatment time and procedural reliability
Improving Titanium’s surface activity, osseointegration and fixation
Based on the discovery by Swedish Professor Per-Ingvar Brånemark , Titanium can be successfully fused into bone when osteoblasts grow on and into the rough surface of the implanted titanium
Dental ImplantsWhy Titanium?
Research Background
The success of an implant depends on the ability of the material to attach with the living host tissue.
Improvement of fixation between implants and hard tissue can be achieved by coating the metal surface with Calcium Phosphates material or Aluminum Oxide.
Recent studies on a series of protein coatings on Ti have demonstrated their effective roles in the stimulation of cellular responses, such as attachment and proliferation.
Research Background
Collagen, being the main constituting protein of the hard tissues, has the potential as a coating moiety on a Ti implant.
Hydroxyapatite (HA) coating has been particularly well investigated as a preliminary coating for collagen immobilization on titanium surfaces, because human bone is mainly composed of HA and collagen fibers.
However, the chemical bonding and the composition of HA-collagen is recognized as being difficult, because it involves two dissimilar organic and inorganic nanophases.
Research Objectives
Experimental on Surface Modifications to improve Titanium surface activity
Immobilizing Collagen onto Titanium by using coupling agent
R&D Flow Chart
Substrate: Titanium
Interface:Coupling agent
Coating:Collagen (type 1)
Dental implantsOrthopedic implant
OH radical
Surface Processing
Collagen coating
Cell
Attachment/
Proliferation
Materials Processing in vitro Tests Applications
Research
Methodology
Ti discs
Polishing Treatment
Heat TreatmentTFAA treatment
& XPS observation
3-APTES grafting
SEM/EDS Observation
Collagen Coating
Cell Culture
Blasting
Chemical Treatment
Surface Roughness
Measurement
Analysis
Research Progress Blasting treatment with HA
and Al2O3 was done to compare the characteristics and to observe the cell growth.
Etching treatment with H3PO4 and HNO3 was also conducted to find the effect for both blasting materials.
Surface roughness: The surface roughness
average for HA blasted discs are lower than Al2O3 blasted discs
Etching reduced the roughness average as it removed the embedded blasted particles.
Research Progress The HA blasted Ti discs showed the
presence of calcium and phosphorous on the surface of Titanium.
After HNO3 etching the calcium and phosphorous were all removed from the surface.
After H3PO4 etching, the calcium was removed from the surface meanwhile the phosphorous remained, it was found that titanium react with phosphorous, confirmed by XRD.
The Al2O3 blasted Ti discs showed the presence of alumina on the surface and it remain on the surface after acid etching, both with HNO3 and H3PO4. it needed stronger acid or longer etching time to remove Al2O3.
Fig. 1 Al2O3 Blasted #200 & H3PO4 Etched
Element
Weight%
Atomic%
Compd%
Formula
O K 39.37 65.69 98.29 O2TiP K 1.71 1.47 0.00Ti 58.93 32.84Totals 100.00
Element
Weight%
Atomic%
Compd%
Formula
O K 40.05 66.67 100.00 O2TiTi 59.95 33.33Totals 100.00
Element Weight% Atomic%
O K 30.60 55.38
P K 4.88 4.56
Ca K 9.09 6.56
Ti K 55.43 33.50
Totals 100.00
Table 1. HA blasted Ti discs
Table 2. HA blasted - HNO3 etching
Table 3. HA blasted - H3PO4 etching
Research Progress NaOH & H2O2 etching
were conducted to obtain OH- (hydroxyl groups) on Ti surface (see table).
The etched Ti surfaces were immersed with 3-APTES, as coupling agent between Titanium and Collagen, with 3 various time: 10, 30 Minutes, and 1 Hour.
Surface Morphology of the treated discs were observed, after 3-APTES immersed, there were some difference on the NaOH etched, but not much changes on the H2O2
Chemical
Temperature
Concentration
Time (Hr)
NaOH 70oC 5 M 24, 12
H2O2 80oC 35.4 Vol % 48, 24
(a) NaOH etched (b) NaOH + 3-APTES 10 Min
(c) H2O2 etched (d) H2O2 + 3-APTES 10 Min
Research Plans
Perform heat treatment (after etching treatment) Observation on the Ti treated surface morphology Confirmation of what compound are formed on
the Ti surface & how many OH- groups formed on the Ti surface
Tri-fluoro acetic anhydride (TFAA) treatment X-Ray Photoelectron Spectroscopy (XPS)
observation Collagen grafting Bone cell test, to compare the cell attachment
and cell growth on each treated Ti discs
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