Studies on the Use of Differential Centrifugation … ~ .. lUtd..,lbh'. 'tri.l11.., ,..u..w ..... 841 .... ~ ..... pt_ a ~ ,.u. tittuo '~, .. ~ aN C1'\d. .... ~.l.e.t ~ ~~ • p"*_tt.ou

Loyola University ChicagoLoyola eCommons

Master's Theses Theses and Dissertations

1955

Studies on the Use of Differential CentrifugationProcedures for the Measurement of theIntracellular Distribution of Potassium in DogHeart MuscleRene Richard KempenLoyola University Chicago

This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion inMaster's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected].

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.Copyright © 1955 Rene Richard Kempen

Recommended CitationKempen, Rene Richard, "Studies on the Use of Differential Centrifugation Procedures for the Measurement of the IntracellularDistribution of Potassium in Dog Heart Muscle" (1955). Master's Theses. Paper 882.http://ecommons.luc.edu/luc_theses/882

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of the cone.a.tlon or poftas1-..111 .-ell 011 fi-fu~t10l:l . .,. 81'"

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va tb. Mo;,peo't t.o .~ ~oU._ ooct41n1~ a{~.,quate of _.gen tbtl ana1ploal .. 8u1t# obtdMd. by th. ~Kj.l4ab.l

~'.mat' be tl~~41bl. with. ~"o'bt4tl~ b'1 tlW ... '1 ...... . -

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port~ Wbt·dh ~4 lnM-o ... that the ,,- _tbo4Jl eou14 p.

lI .. atl.u., ~_ petml ••

lm.tMA at fA_ tB, .DIW". Rlts~· . ~. of til_. 4o.'1tUnlQ$ ~ S to 150 .,..~ ...

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~ Me. .U'OU. '~"~..aJ19 ~ptn _l!thed and the _igbt of

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tz.'o ou r4 or .. _tel'" bomoaoaaH (20~) ,18 ... dde4 !.!) 114 at! coW ulCk"lloN •• ,1 ... 14 (tell.) ad tne; .ut~ 1$ e_t:r!~d.

lfbt ~'~fI 1s Npat,~4 with 2.5 m1 ot 'fCA d t.~ ~RlAW.

1thi~oontaln tt-. ac14 .Qlubl. fftOt1cm aN d1_~.4. 'lb. tol­

lodUS 101",.. uo ~lsed ftut'u'J ••• 1ve17 wIth. ~ aat.a lf$olublo

tnDt!:em Qr ~ tl •• ~ _4 the .~ld.~ ._tFlru~t\ ·la.ori.l'"

to ~,. t'be pboaJPhOl.lpUe. 1) m:. It1 ftto,.. pta 4 1'4 et ~".

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Wb1QU 1$ ~ thP •• ttl.a. 'fl,. ft8t.dUfll; 1. 'tb.ntnatM r_ 16 110 ... 8 at :rro 41' with 1 11 K08 1a oHer to ~~11 •• tbe riboa

f(.>j.'r'

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6 • BC1. Ot. wl ... of ~." 'lOA 18 added, and .. tpltuged. fU

pHo1p1 tate 1. mlab8d ono$ wi til :$ 1IG. 'Of S" ~A ad th$ JJ1~te:.

are ea.biM4 tOJ! ~ 1:*11» •• a."eJIlUl'lAUon of ~1bonuol.1o did •

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d.I.~bo_ """ aoo.ac~.ag t» vo!J4a & Collm (195J+l.

a~tlI

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cepuaR4 ~. tl.#wt homo~ •• 4 1~ lacton1o"l"'O_q~ns

to the 41:tf1t1HtQUal Mntl"1tusat1oa _tbe4 .t ~~ fit' a1

(l94Sl. the 41.ti"'bU\tm ot »oflud.a., altMsal1, d weU d tt»

potul'~be_ nth. 11'1 ... oell tr,aeU ...... __ • In !a14t,.1. to'.nl_ ~_tN.U_ 1 •• .,HeHd In t ... 01 rtdtU.

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s. ~ to _.~'n the Ho.~ or atmd at ehll

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II

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of ·tho ... 1f\Ilo dt4a .. !)e~~ _Nwlu·~ t1ut.t ti*"

$~ to be· Q .ellgbt17 ~JlGoneent~tlon of th4& ~l.!o ae1da

in tll'$ ~"".Qt tbe '""tid ta._,. ... •• , ..... 0ft1,.

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... a.t1 •• In tb&l non-b.~pr.d.~.d t1.rd.$~l.$ ooo~l. ~.

tween b41ft~' b$~ _.1"''- ~kab17 ~~t in the ~ ...

1maat ... 1~ tbeH ••• l,wlH'tton ~.~ .fc>1It a_sea < ___

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'\fU*l~a.

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ft1Ca"OM 1a the MaUl. t..~ •• 4&_ .... one~ _ bet 1Ma

eN4l •.. d .. t ...... t~ lath.-~. " ".,.J, ,

?OTASSIWi CONTZf£11 Cgt,tFRA(7flOliS 01' aBAft ~IsaDB lWMOOIIX= AIm Sm'AMfiD BY !BE BOGEIOOM ffroCmmm;:

liOUOOaua ~1

"* ... a 'NN J .--

1 _ • (

D IIl.ifOIIC SlJOHGSB

_Al _114 1 I), U.Ii I .1.1111 AWl II .·1111 1 I ", •• K IIU,." •· •.• 1, 'I

78.S 2._ 38. 19.5 1.9~ .. . I "i , 1 JI' • . Ii l • I I • !.' I 1 'i "'MIni!: 1 J

60.6 1.99 39.3 ,,-It 1.01+ SO.2 2.0) .l9.S 19.1 1.00

,1.5.1 9.u ,.,riNft. .JS.~. ,u.a ,iii! 78.6 2.OS 38.0 18.6 2.01

I 48.9

I . q'Mti! I

39.1

39.S

JL.a Ii~Gy'

)9.0

K. I . III '1 I' 11111 .-J (.JIlI.) .• U "'. r, " 1M ~ 1. tI .1 .. t l~ 1 f $ I, ,,,1,1., lilJ"!ilI. ..

0.11 n.bll'U ~l ~I 'fuba ,

, AVO"

....... IA ~l ~I , ~l

; AftJl~

~ .. =1 T*tJ

4 •• ~

~0!A.tS

" Reo"v • .,.

it.. ""~1o

2.6 0.11 18.$ 3.0 0.11 la~o

..Ad..JW1 ~.A c'2;I'o~i6 17.9

. ~ .••. '-. .&:ji la .. 35

. -zt -= 'td!8 16,.2 2.08

91 101

Jl • Mos.._/FJ.l.

.1, .6. .... ' :.6 ,1.* .. :.01 1 1. D, :.*k. 16..0 ,.,. 13.0 -1Ia2~~ -15;' ~ JI;lf

i~,m 81.5 2.11 lOl1O!)

!til .. 1I$q. Jr/lRq •

JO

JOTAS:3Il1A COlrl4i:SlT II CELL FtlAOTIOllS OF BBART TISS'UE BOIOGBlZm W%TflOW AmI!I01f OF Dlt'OttIUG ~D

ABD SUAft~f!n) 'Sf' :FORCES OJ- 10tl,OOO X o lei JOE." •

m!.~~!4\D "l •• ~ fIt,1*.""141 dlt. I"

!RECIlt!A" ' V~~1_

MfA41e ..

.. AS

1' .... 61.1

10.$

1.91 2.!4 ~,.q.

39.1 )6.2

36.0

o.u ~_l. .1~lA .... ate if 1 _ 1M M till ' .......... ,fl .• "I.1iI li!iI.wllfl ..... vt' ;;'I'lI.~J .• "IIi. til" ill •• q.I,. .... lIif<ll~ ... t I'~I. "'"_.~ •• f I.'" _ d • A .. , .... I..,tt· i I I"

1.20

1.45 1.43

0.29 0.)1

0,.33

0.58

O.S3

O.so

J. • , . B .K .. " VII""' " t .. '

_1lS U~r~

Baa.. .. Bot ....

I ••

~.29

a-JZ

-".,' I I 'i_"!!a;.

105 101

lOS

PO~A$Slurki O(Ji:l~mft' III C»JLL Ft"t\CfltIOli:S OF HBArlT TIU8 HOM~l~ WlTilOtrf AtmITl()l:l OPDltt1'll1fO FLt.."XD

JUil) lD.L,q,\f1D BY . OF 108.000 " G 1'0,1

raJf. 0.84 16.6 .a~9 1.01$1 .. 0 Ii 11 U" ,_ " 1.)1 .1 ... 1 U r ' IOJi • ~IJ.I« 1 It II \ .... U1 .• ..... " ••• .JI. IJ ttl Ft'. 1:1« J'

:~J. 2:.81 ~ ... 2 2,..06 36,4 . Sat a~09

.tidle "> ~9 2+11 .~ 83.3 _17 3S.Jt, Bottom .. 65.0 2'.31 28.1 ~>2 .32 36.3

BOG~ avARA'flvllS 0" Up,.. ItIDDU, Be BOft01! PO'ftiO'HS

, ••• '",,(1) !fl,inu" "f1 .... tau. ~'111 ~t ... : !i&-W~. 'iii. . lUi' ttl

_1.L6 uppel' ,.,'lon .

1 •• 0,,26

.~ ft 1;60 > •••• 0.27

Bo~tom 1.60 0.2)

; "!It ........ r·'.~.M !

J~llIfA'8. ~po"'on

~ ft

Bottal ..

K .1M/K11o I. Bqfr\llo

90

106 lr4

If/B .. 1Mt1 I'/Et •

, 0.66

O.~9

0'·$0-..

NFl .-.- •..•.. '1(11,1 lAd

90

100

109

.

mI' • "- or AV .... p DlA • ".~ J\"~ .

r,arr; f •• ,1 WHIJlli "je', far

~lWU_ rlld41.e '.

Do'''' tt

Yoh.1 _ 1'.r (t " INr ,"".I! -'d' I!lt ••• _ jlr! _"'31

2t.J& a.15

I. ""'a/K1lo

1. 101

II

, 1

1

!he u... of tbS. lnft.tlsaUoa .. to .'u4y the dl __ s.., ~. of potuab. 1_1410 tbe· eell. ~. ftft1tect thl •• tudy

indi ••• that d1tt~'ta1 o.t.ltupthm ,... ..... sb10b _pa­

Mte ~ .-11n1_ ~,. ~ _t be 4ft a~h ... to. attaekS.q t,,~ p<ibl.... .u ~ .. t84 pH'flo_lJ' in tbl. ---sa.

q

.t;bemt..,. dlttl0t4 ........ be· b ~a1nty that' "·taut •.

~ . .,. ..... ,1_ ~1 ........ or .th,e bae'leutkn

pNO~ • ..,..~ wl\h the _01* .~ .... 1 ... ot 411\\t_

.t.1ufA.

»UP!_ t,hb 41tt1ouliq-. a .. \IIb ~'1 .. app8aNd to

1M otr_ted ..". tho ~u1.. ot Rogeboom ed... tbo )pOtua1l11'i\1'

nt,"_"'10 ......... '1.117 d1tf'fWNn:t b tI'l .• ~tlO1US

.~. b Id.to.llO~a ba4 .. lotre:t:t pow81,.!atbos_ntto

'~ ...... 1. tl'" -= this ftp. sl.lht be ...u4 .... _tad

.t. ..... on the _ .. +4_ tb.at.-~ ot potaut_ tate the·

I'ld.tO~d traetlcnl!4 _t ~ place at a :rapid Nto. A

'1.1U'il~,'lOft PN'ba1Jt, holda' t01!l' t,.~ cell 4&b'l."!. fl-ution

r 35

since its pote.8alt:rrVt11t~oBen rlft.tio 1s lower thral'that Q,f tho

who].o tiaultte but the h1$tolog:ieal het6:Nl~Gllel t,. of this fl'9.ot1on

1"&ndOPfJ ,1:t:f'fio'.alt AI'!'J' fnr 1"~ac;h,1nr:: 6onolus1t1n~ to 'be r1.'lr.!e. 1'J?ha

cont$.tu,nation 01' the 81Xf,Hll"nl'l\t$: 1!'B.ct1on, for e)ta5!\pl$, YlU1Y bo as

high u lOO$'; aJ.:r1f.le the 1J'~f)t _l~ht of: t::r,1s i'rn.(, t1c:n1. ia about t:~'11ce

thn:t of' tho ol"'lg1n4!l1 tissue. 1'.P1l$ cor:t"{)ot potassJ:um/nitrogen ratio

obtedned for the $u.-p&matQ may also be sub,ject to eor,\t:S1~!l<J'Ptlbl$

G1'Nl'Ol!.-. Ono 1mpox-tant faotor "<wul·d be tl~ gain of potus1tt~ &11d

tUt~een lostfltiom t00 oth." e~.nta. It 1s "nO" 111(8l3" ro~

potassianl to he ~~t.Jtaot.d t:!'lAn n.1ti"t')~ll alnee th .. prote1n 1OO1$ou1e

would b, le •• _bl1e~1 le$s ~t to penetpat$ a. ~8t:ri,ctlng

s~ poS$1ble that a p~tl't1.1111 •• p4I'at1on m1~ht be eri'",~ete(l by

oentr:U,'u.~~at.1on 1n tll$¢\bu~M$ or dl1utinfs flu1rl. 'h15$ pPOcodtlre

was not suoeoastul. P.~hl:lp. 611e "'Ulon is thn t 1"'or an o/loquat •

• 9p~tlon, a eerta1.~cl atOOlmt Qr tllad volurl\$ is neooss,arytor

d1i"tel"'Ontlal loontlent of the e~Mnt. to Q.,t'i1..U' und.9r the p~ ...

sent o'Jnd1 t1on. It ,t;:2.lG "nt~1tll.gat1on tiM wa_ utended# the

<l11ut-

1ng fl'U.1d ldght bave b~.11 OVGPOOtllCh A $ooond. r&S::lV)ll !AAY he that

aggrosa t10n or tho 0$11 eOi.npotuwts ooc~l. It l:t:;cs been r'oportod

r,...------------that {Ulut1ng fltdds which cont&ln elact,rolyte tend to~r:wezat.

c(t~a1n cell oompononts (Eoe~l}QO~~l,. SUhnett1E>:J:II. &' ;falladtSl, 191+8> and It t:1J.1'1' be possible t'b.at tr..eoon,ta.ct ot tlxtx-ae:ellulu fluid

·wl1t!.l 1ut.;t'l;!loelluln:p m&a.tf):raial ~ haw be-$:n su7.'ftelant O&,U${) to

pro!lOte afMweg:at1c.'!rr.:'l. in the :pl~:30nt system.

;~ltt~~ the proaent datil. is aubjaet to o(lltUJ1(!o:P«'l.ble

~~ e~l"', it l~Ut:r b. pos$ible that son. or the dtrta obtained

\1'1 t11. the liogob()('.'P..n _tbod or thetii.f'taro.-ntinl p..t'tH,:dn~ method aM

aotually olono to th.e COrMet valuos. The at:1(''\lm.t of' potassium

touml In tho l:l!lltooMl'ldrial i':Paotioll could b$ l\ t:rue &8t1mat$ of

the tmrotmt jft$sQclated vr%t:i thts oomponerlt If it could be a$3u.u~

tl~t th~1'e waa 11 ttle mo":,,e1!'it~nt o:r potassiUJ:51 <1'1.,u"':i11e the ;t":r-fi\ot1,on ....

at1on., ~~ 0011 debris .frm.ct1o.u baa a oons:i.del'<\o,hle &<"'I1O\mt or

atll)n (i.e. t.he eU1'(}mate) -'.1 be 01080 to ttl. t'l"UG :tig:u"._ 'l~

supema.te 1-:tllB a ldgh potaasltmt/n1tl'O~ I"a:tl0 b1Xt it ~lta)" bo 1')~~

fe()tl:r ~~onabl. to flrul rilOst o:f the 0011 potttIJ8:ltt':l tn the cyto­

pltlU)~:,! Pl"Ope-l.'l. !,b\l{~Vel'f it 1. undouhto~11::r moPG judicious to as",

o~'bo loss than moM- _an1ng to tho dm:tl;t attne 1)l:'"esent St4f]S or

our knovledgfJ . .,

TheN I.PJ)oared to be SO~~'!.0 oVlda:t16a thl}' t ~·':1iej.>(Jsomes W6!lt!!

la:rCl"'Od on to the up;)!:;r!· portion (.).f tho PP(~c! 1;i ttl. teal during hi f~h

f1.$ld. If th1.8 ~.ult YlOl1O tob$ .s(uJ>1.ood to tl::e p);-oaewe ot

l~gGr &1ounts of Stlpelilllattl. it (Hl:rl. 'bo calo\,tlat~i tb~t. the potaa-

point.

COl:1Ccm.t.r:slt:ions b".1 ~l11tor:b1.f,j ,the volu:t:.te of' dilutlnr~ .fluid. It

PQtaga~"tt::J. oon.el,)ntx-at~to:n. 113 'Unfll t;f!)l"ed by eli ltl,tlon, t::ua

to

radioact:l ''''0 potat:)s:tu.tt4 II: thl'; lffi.tea 0,,[, erXCh&lf~ ill. lenT, ,. t nlaY

be possible to eone:tdw ttl() data in this th:os1a AU relat1vol:s-

v

1 ~;~"""" /14'i<d"··"'''!\~''1·t·c1''''''n ",#l 'r'''''·tJ!'1",,~'l''''''''''' nnr"! "l·t.t·-<·",..··'''''?l 'A~"~'" .. , ... 4AU ~~~iI v'" _-..\4 .... ~ ;L..'\...:r.... ~J,,1 ~\JV .o\i~u~.J ... ~"<", '.:h ~ ~...., ... W"""t,'.'J SJl·~ li'if ........ ~

'I::'lOt1:alu:z"cd in c~11 fI-a.'Oti<,ns 01" thG dOi~ '~""firocCl.r<l1;ux:;t ohtained by

the cell ;et'e::t.ct:l.o~:".j\.ti()n teohniquf,:.l ~. \.. t 1 .,. t iJiOeOvOOt1, e· a.. ;;:~os,

of t':H~ pot~"u:lil;h:.\tl '(,!f(Ul found 1Il tl'le Stl.)~)!"DJ:lt{"}

deln."is. "d. t{)chondttia, tJl}.d SUP(t!"llG. t>3 rl~$U): U.'llS

!'~cn :r.to.tloa 1!, ;:: 17' '1 • (./.1,), •• _, l ?~> ""{"au~ct1 vel'/' < l..""'~.'~' ,4 # ;,:' . ..... ',,~f •

~ 'M. ""'~J).'iI'j. t;::ltif':';';al f'Ol"!HHl, , (';'. '.;1f1 ";.,,. """"'n1 .".,,,,JI'o t'l I'> 1 "I') ., ... \..;1., r,,:!¥,:;.JT,,'" ...... V"'';''~,,, ~~""',J~A¥"k ...,·.",~_U·~" ..l • .},," foci; a

ti::)u. 0·011

ootsum1.ll7'n.i trc .... '" v

....,.., w .• 1 l('{\ t)("<;"l .. ~ ~\\, ,.. all •.. ,;. ,P.} , •• \J'". A

t1.ssno t,1 t:!.on of a d:l1ut;:lnr fluid.

i\' ~Up{)l"'U~te and. t~1tO '1;}01:""0 obtl::d.ned but 110 1"'urth{yl;" flt)~:H"l."'a-

t:1.<:>n 0 i"' eoll eO'!11pOllentD 't4an (lbt$l.lno~B as {:1~t,{)):~~:d.Md by fractIon ...

{~t1on f:trl tl~ i;Og~~ p~')e$dlWe or pr{~c17:)1 tr!lte $~l;r"iple$ ts.1.';:O:l:l

~:'11 vax"lcn.:t£j I:'Lre&8 of.' t,he~>re¢1i·'d.tat.&. fl~(~ d.ata doC!u~, bcnJev'Ol't

il1<11CQ.te tl'ltl t II ,?oo!'dble s~;lpnrr;;; tlcl1 :::. {' t::~o oee uJ;1I;:::'ed

ltl tJl0 ~.l1'rle:C:" :i<n~t.ion of' the pr~ci1'1t$.'te.

3. III one E:lXt)eri;';l$l.lt 'Uw dlatrilJut1()!1 of tluclol.o ac~,dg

~'11 ~'}H'> ",.,:\ .... ,1· ""~" .. 'H'''''''''« ;'1'(" ,hA n'li:tA"":1,"~1 tate \,iet"'Q d;'>4,""''''''''''t'''''.~,.", '''''''''''''0'''''''''''''' ~. 1;,.1""'010.''''- "f\o,~. v\..~~ .. ~'~~.;:l' ~~,.~, v.,~ ir,h vv '}S"- :t,tF ~iti;#4, ~.5...l.."x..;',A·t~,:pli.~. ~~~ 4'''''''';,

ribOlltlOleie ['.aid ;'Ins dtst!"'lhuted CrllJ!Hlly tlu:-oughout the p;~~eci:'\lt;.a.t~6

Ii) ~

~~'lOvo;:wnt of: pota$~dlml h0tWGOn ~6'l;lml. d'U3;ti:n.g tJ:lO .fracM.onation

p.tclQoedu.'t~1a 'W;OlOr.m. Fu.t*th;()~ study is n~003$at-y' lwf'ora t\ tj'l~

!ku.":n.t~, C. :;'., f;:. H'u$t)llrJ, 1 • .;'1 •• , 191~3. titatl';.:~e ,'\:::;'!;'l.l;;'itJU ot the l~rt:teulnt;e ~tlons tr~n t'ious~ IJ.v~r Cyt(:)pla.~!l. ;~~. B!,oeh$'lu. 19:17.

13~U'tll.. c. ~:. _) & :Ut:tsabl.:~. l~. ~ 19$0 It 'l:1,1.f) In. tr"tlQ.$ 11 u.lat'" Ha \;~)l1fQ-aorJ.Ci ty Qr .l:'ontotllJ :Jno1010 Ac1.d ClS ~:'fy"1d"'j:1.e{)d tho Inco'l"',Por-atiz)n of' Hadlo·phosphoM.ut. .~h. Y;;1oeho:n. 29:1.

1307&1', Il. D., 19:;1. Th.e ;101a of :i>o ta3si 11X7l, nnrl:1alf~tJ,))d Catiorl.s in tb .. Aotion of, IJ~v:10 Phoapb.().r~n~ue tmd Ot!lGl' .!m~oo. J. ~t 13119S.

BriIll!:J ~';\ .... Tr •• 195). The tlole of" Po tar HI, ner"'.re (j()118. J. Lancet 1::)1171.

F~.l 19J1t, , H()seho:t'Oheu stu". 1& HOl:Hlrt: ticn du potamd.u::l <1&lJ!J " e8 OGllul~a et sur 10. {'i"pl~,~¢a~lellt$ qu' 11 $ub1 t au cours de. ph~nb"':1:l8:ne,s d lexcetation. Ar*('}':l. Int. Pl:li'8i\~ 1. ; 311.

Cmtarow, A., & SchepN:'t7, D., 1951~ B1001~$t17. SaUll<lers, Mladelpll1a.

Claud'0,t n., 1946. Pl"actlQn~t1{~l.'). of Uleln;tiUllirul [,i vor CellsD!t· ... :[~():ventinl C~nt:ri,fl.tfJa .. tlon. I. .Fl'"'oblo,:;:,!s, I1ethod. fl.nd PNpu:vatlo!l of l~trllct. II. l:~~~ntf1~ l:'rQcadm'"os and ~lellfulta. ~J. Ex:t~:rt. t£OO. 60t19.

Claua.o. II.; 19Q.7-48- ~~tudl$$ on Cells: V;tOx-ph-ology, t'!l:~f;d.e~tl ~18t:i tut1:,:n, wld D1atriln.rtlc'rl 01"" J",iocherncal ~~i\mctlons. ~y Lectur •• 43t121.

P., 19!a. *.i?O'poel10r:lisha tJlltarauehtl:nr:~erl 'fIber ,::n.l:l:U;:;l tllll;d !t.SehG~'halt :1!1l ;'ltU*[;wUitol"'her~Qll. ,fl.nn't. !~9dl-Ol. ', ..

iJ'xtn.por,. yr. u." b liodc~, A. J.# 1950. E.'looi:.I-on-1:o,di.1Ccd "11oroineln .... ortttL~!'l ·;:1.th tho:lf)ctZ""-:m i:~:teIlosc(mo. I.:--:lr:;trib:'J.tion or:~Gal-dual I111U:lral OCJ!ltont in Vetttobl~n.te St.r·iutod '1uaolf>. t!"n,lt!t_n tf. .::~x:;}~r. Blol. t~ j~. ~;c. 20: ::·;ll.S?

I>.l'btuason, ;,r. J 19!f2. ::'3\n:- la !'~i;y~;t-.tl tic;'!. des ion.s da.nn lS3uoelo ~tl"!l6. ,!\;tlO11. Int. l~hya101. 111 ;!t.,39.

,j. (J., 19S3. Introd:'lot1on to & ~.:~os1u;n, on the I'letabol:ts;;l orpoti'ltH~1'l~1. J. !.!n.ncl;lt; 73:163.

I. I 19)8. 1l1$Prov(;Hl Eistoche;'1iCl'l1 7,tottlo(la ""t>1" Chl<nrldo I ~wephat;$-C~~;rbonate O;\.."'ld. PotaaallJn1 ~.p:')11ad 1.0 ::r~:ol~')tal ;~t}l... "~14t. aao. 10a311.

ClOPSl:l, I., 19h,1. neo~nt D011'61op't!lel1t3 in Ht3toe!:lc~"J.lltl"'Y. Pb.jsicl. i1ov. 2l121.f2.

D. 19$1.

G. B.., Sehn$lde7Jf', ,i. \.;." &: Pallado, G. 1:1." 1.9,"~. Cyto­ChOll!ic,,:ql Stlld1~a of' i;!a:I '1a.l1 rW. '~,'5,a.u9; I. Iaolatio:tl or T·"'t, ",,,,.~. '!;tf·t ....... ·h·""n{'t~ ... Jc~, $>· ... r'~". 1}"'t l' i"''''''''' .... • !:-:·l""·Ct~", ... ,·,~ ". ,-1 AJ,-J, i,*~~ ,'~(~ ·V¥4A'\R ..... ~{,..% ~t..-,:§.}. f' )"."j,,./,\j\, ).:r.,ti'~ ,u.,,1 VV ... ilA ~ .. "J ' .... 1 't,.~.,'"jF_,._"y~ .. ~,'

:Pl"OT)1~1.,t1es :2.1 tc)Chot!.i:lriri 2m.d;~tf:,:~:TI.1cl:."'o:Je(2)ie P.tl1"'t.ieu.la. :,lntOol'li{ll. J. Biol.:;tH!}I~l. 112:619..'

Iloc;ebo01U. o. ::1., 19S'l. !.~:;)~.lr!1J,tit,:)3:} and ents. .ZJ()C. 1(;'6!.}D.

.• E., 19~~1. ~\l;n"Aove:r;t 0:.' .i:'l--otoins 0:[' Cell ;Ct'c .. (.! tic,)D,a .!\Glult ;C~e,t I.t:1v~r 1n ·vivo. 1~d. 7-'1'*00. 10:206.

r

t~ ""#00.1"'1, ,' ..... . ~~ ~.~~&.

10. '.:2. :::''''''·'·''>h·,'\at~ o"]nt J'.", ,,~·.,"t;.FtJl,~b "44.'" ...... ",-,<

:';orr>hol0 ;,·S. c. t1.l1y tl.l.ld ttl" 1 ": .. CL~L~C01't ~~o,;. j",",~'::(,!"/G.

t; ::aossi t;Ol". T'i3m!& t.:'r(J'm

/~. I3., 1{){)5. {)1::' tlt(l 2l!'st:'l-"lii.)t:tlo11 cf* .l'·ots.~:; Ani.;'lal and. Vegetabla OG110. J. 1::tr~Ta1Ql. :'';!5.

H. J' 1953. Col1'lAlfU~ T:Gohani$t~1 J. Lancet 73t16~).

O!'l\ae~il. A •• Hf:tl"l'1i.:l.til. G. P. J t,~ ::1110:;:, D., 1911.8. ·, .. ~ua!lt,:tt{f\tivG 1)1.$­tl"ibutlon Gf ro.1.;:~$t.ra~ a;'OOXlr:; Cytoplt~$~'de Co!::tpon":~d;t'S Of r"lo~e Liver Ctilla. ;:~·o'O. ti\oo.·~~pa.r. B;t.ol. ;lad. 67:133.

Paig$n, It., 19$!,~ ~'h~ COo""u'T'$nae 1:)1" .~V$rnl. D1oeh,etld.ca11:r ;lti:~ tirlOt'~.n4$~ (;Ii' 2a'l.A)ohouJl"ia in Hat Livor. J. 3101. (:;l:~o:'1. 206 t 9i~;~.

?(')llc!;u'*d, A" '" \:.: Pillet, .1) •• 1928. A~11,cat1o:n 1'1,0 h1$toe;.d:.'Lni;,fut:) d() l~ ~J:l(n~,':tncln(\rtli;;~.c:tl 6. pi~J'TI$nta antraco&lqlte.s puh.~M1r0s. C .. :1. 93':985.

(;,llQ

dos ~)oe. 13~.ol.

,"\ ", '\'" •• " .,,,,~ "~'"'''' .. "f l()tl ~J ., ,i""} .... t'> ·i~" .• >.·":l'.'lI_ 'A_,. (,..., ~~.-:... .... {""''''.1".;1.;,.J~, _":J., ~.# ""'V;";I* .. \.~ ... _ ."i.J".l '-

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