Structure & concept of Structure & concept of gene, gene, One gene one enzyme One gene one enzyme hypothesis hypothesis , , Genetic Code Genetic Code PROTEIN SYNTHESIS PROTEIN SYNTHESIS , , Regulation of gene Regulation of gene expression expression Dr. Madhumita Bhattacharjee Assiatant Professor Botany Deptt. P.G.G.C.G. -11,Chandigarh
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Structure & concept of gene, One gene one enzyme hypothesis, Genetic Code PROTEIN SYNTHESIS, Regulation of gene expression Dr. Madhumita Bhattacharjee.
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Structure & concept of gene,Structure & concept of gene,One gene one enzyme One gene one enzyme
hypothesishypothesis,,Genetic CodeGenetic Code
PROTEIN SYNTHESISPROTEIN SYNTHESIS,,Regulation of gene Regulation of gene
expressionexpression
Dr. Madhumita BhattacharjeeAssiatant ProfessorBotany Deptt.P.G.G.C.G. -11,Chandigarh
Definitions of the gene
• The gene is the unit of genetic information that controls a specific aspect of the phenotype.
• The gene is the unit of genetic information that specifies the synthesis of one polypeptide.
1942: George Beadle and Edward Tatum
Studied relationships between genes and enzymes in the haploid fungus Neurospora crassa (orange bread mold).
Discovered that genes act by regulating definite chemical events.
One Gene-One Enzyme Hypothesis
Each gene controls synthesis/activity of a single enzyme.
“one gene-one polypeptide”
1958: George Beadle (Cal Tech) & Edward Tatum (Rockefeller Institute)
Beadle and Tatum (1942)--One Gene, One Enzyme
• Bread mold Neurospora can normally grow on minimal media, because it can synthesize most essential metabolites.
• If this biosynthesis is under genetic control, then mutants in those genes would require additional metabolites in their media.
• This was tested by irradiating Neurospora spores and screening the cells they produced for additional nutritional requirements (auxotrophs).
Beadle and Tatum proposed: “One Gene-One Enzyme Hypothesis”
However, it quickly became apparent that…
1. More than one gene can control each step in a pathway (enzymes can be composed of two or more polypeptide chains, each coded by a separate gene).
2. Many biochemical pathways are branched.
“One Gene-One Enzyme Hypothesis”
“One Gene-One Polypeptide Hypothesis”
Modern Concept of Gene
• Until 1940, the gene was considered as the basic unit of genetic information as defined by three criteria.- Cistron:The unit of function, controlling
the inheritance of one “character” or phenotypic attribute.
– Recon : The unit of recombination– Muton:The unit of mutation.
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Genetic code:
Def. Genetic code is the nucleotide base sequence on DNA ( and subsequently on mRNA by transcription) which will be translated into a sequence of amino acids of the protein to be synthesized.The code is composed of codonsCodon is composed of 3 bases ( e.g. ACG or UAG). Each codon is translated into one amino acid.
The 4 nucleotide bases (A,G,C and U) in mRNA are used to produce the three base codons. There are therefore, 64 codons code for the 20 amino acids, and since each codon code for only one amino acids this means that, there are more than one cone for the same amino acid.
How to translate a codon (see table):This table or dictionary can be used to translate any codon sequence.Each triplet is read from 5′ → 3′ direction so the first base is 5′ base, followed by the middle base then the last base which is 3′ base.
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Examples: 5′- A UG- 3′ codes for methionine 5′- UCU- 3′ codes for serine 5′ - CCA- 3′ codes for proline
Termination (stop or nonsense) codons:Three of the 64 codons; UAA, UAG, UGA do not code for any amino acid. They are termination codes which when one of them appear in mRNA sequence, it indicates finishing of protein synthesis.
Characters of the genetic code: 1- Specificity: the genetic code is specific, that is a specific codon always code for the same amino acid.2- Universality: the genetic code is universal, that is, the same codon is used in all living organisms, procaryotics and eucaryotics.3- Degeneracy: the genetic code is degenerate i.e. although each codon corresponds to a single amino acid,one amino acid may have more than one codons. e.g arginine has 6 different codons
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Gene mutation (altering the nucleotide sequence):
1- Point mutation: changing in a single nucleotide base on the mRNA can lead to any of the following 3 results:
i- Silent mutation: i.e. the codon containg the changed base may code for the same amino acid. For example, in serine codon UCA, if A is changed to U giving the codon UCU, it still code for serine. See table.
ii- Missense mutation: the codon containing the changed base may code for a different amino acid. For example, if the serine codon UCA is changed to be CCA ( U is replaced by C), it will code for proline not serine leading to insertion of incorrect amino acid into polypeptide chain.
iii- Non sense mutation: the codon containing the changed base may become a termination codon. For example, serine codon UCA becomes UAA if C is changed to A. UAA is a stop codon leading to terminationof translation at that point.
How your cell makes very How your cell makes very important proteinsimportant proteins
• The production (synthesis) of proteinsproteins.
• 3 phases3 phases:
1.1. TranscriptionTranscription
2.2. RNA processingRNA processing
3.3. TranslationTranslation
• DNA DNA RNA RNA ProteinProtein
DNA DNA RNA RNA ProteinProtein
Nuclearmembrane
TranscriptionTranscription
RNA ProcessingRNA Processing
TranslationTranslation
DNA
Pre-mRNA
mRNA
Ribosome
Protein
Eukaryotic Eukaryotic CellCell
Before making proteins, Your Before making proteins, Your cell must first make RNAcell must first make RNA
• How does RNARNA (ribonucleic acid) (ribonucleic acid) differ from DNA (deoxyribonucleic acid)DNA (deoxyribonucleic acid)?
RNARNA differs from DNADNA
1. RNARNA has a sugar ribosesugar ribose
DNADNA has a sugar deoxyribosesugar deoxyribose
2. RNARNA contains uracil (U)uracil (U)
DNADNA has thymine (T)thymine (T)
3. RNARNA molecule is single-strandedsingle-stranded
DNADNA is double-strandeddouble-stranded
1. Transcription1. Transcription
• Then moves along one of the DNA strandsDNA strands and links RNARNA nucleotides together.
Nuclearmembrane
TranscriptionTranscription
RNA ProcessingRNA Processing
TranslationTranslation
DNA
Pre-mRNA
mRNA
Ribosome
Protein
Eukaryotic Eukaryotic CellCell
1. Transcription OR1. Transcription OR RNA production RNA production
• RNA molecules are produced by copying part of DNA into a complementary sequence of RNA
• This process is started and controlled by an enzyme called RNA polymerase.
1. Transcription1. Transcription
DNADNA
pre-mRNApre-mRNA
RNA PolymeraseRNA Polymerase
Types of RNATypes of RNA
• Three types ofThree types of RNARNA:
A.A. messenger RNA (mRNA)messenger RNA (mRNA)
B.B. transfer RNA (tRNA)transfer RNA (tRNA)
C.C. ribosome RNA (rRNA)ribosome RNA (rRNA)
• All types ofAll types of RNARNAproduced in theproduced in the nucleus!nucleus!
2. When lactose is present • A small amount of a sugar allolactose is formed within
the bacterial cell. This fits onto the repressor protein at another active site (allosteric site)
• This causes the repressor protein to change its shape (a conformational change). It can no longer sit on the operator site. RNA polymerase can now reach its promoter site
Promotor site
z y aDNA
I O
Repressible Genes - Operon Model
• Definition: Genes whose expression is turned off by the presence of some substance (co-repressor)– Tryptophan represses the trp genes
• Co-repressor is typically the end product of the pathway
Tryptophan Operon• Structural genes
– trp E, trpD, trpC trpB & trpA
– Common promoter• Regulatory Gene
– Apo-Repressor• Inactive
• Operator• Co-repressor
– Tryptophan
R
Operon
RegulatoryGene
P O E D C
5 Proteins
B AL
Inactive repressor (apo-repressor)
Tryptophan Operon
• Co-repressor -- tryptophan– Absence of tryptophan