A member of CGIAR consortium www.iita. org Strides in Building Collaborations for Cassava Breeding SK Hahn, AGO Dixon and other members of the IITA Cassava Breeding Family IITA Crop Improvement Workshop, Ibadan Nigeria 8 September 2015 TMS- IBA121635 TMS-IBA121634 including many other National, International and Private Partners with women, men, experienced and new farmers in Africa
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Strides in Building collaborations for cassava breeding
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A member of CGIAR consortium www.iita.org
Strides in Building Collaborations for Cassava Breeding
SK Hahn, AGO Dixon and other members of the IITA Cassava Breeding FamilyIITA Crop Improvement Workshop, Ibadan Nigeria 8 September 2015
TMS-IBA121635TMS-IBA121634
including many other National,
International and Private
Partners with women, men, experienced
and new farmers in
Africa
A member of CGIAR consortium www.iita.org
Cassava in Africa
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Future Potential for Cassava – The Rambo Crop?
From Rosenthal and Ort (2012)
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Global Agricultural Trial Repository
1,410 agronomic variables measured are mapped to Crop Ontology
29,633 trials out of 34,329 described use the Crop Ontology trait names.
CO terms used for metadata and search
http://www.agtrials.org/
Cassava – 1654 trials uploaded with 1293 uploaded by CIAT and 361 by IITA with traits conforming to ontology
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Cassava Source Sink (2015 – 2019)
Metabolic engineering of carbon pathways to enhance yield of root and tuber crops•Andreas Gisel lead •Physiology of root yield •Genotypes: TMEB419, TMEB693, IBA30572, IBA980581, IBA010040, IBA980002•Testing of transgenics in 2018-2019
Partners:FAUETHFIFBTIUniv. IllinoisIITA
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Drought Tolerance in Cassava
Cassava drought tolerance experiment:– Two treatments; irrigated control and drought stressed– Two years; two sites in Kenya; Kibwezi and Kiboko– Five drought tolerant and five drought susceptible varieties
Findings:– 59% reduction in fresh root yield– Main mechanism driving drought tolerance is stomata that
respond slowly, do not close immediately when relative humidity drops, thereby allowing photosynthesis to continue with increased fresh root weight
– Leaf retention assists assimilate accumulation for a few weeks
– Selection for yield is a good proxy for drought toleranceOrek et al. (in preparation)
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Cassava Markets in Nigeria
1 Source: Context Network analysis based on field research, farmer interviews, and published source analysis2 Garri: “CTA Presentation”, October 20143 Industrial and Other Processed Food: “Sahel Rapid Appraisal of Nigerian Markets for Cassava”, August 2013
Nigeria Market Share
(by Usage)1,2,3
52.4 Million MT Production (2011)
Boil and eat – fresh consumption
Garri
Other Food Products (fufu, lafun and others)
Industry
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Processing into High Quality Cassava Peel (HQCP) Mashes
Slides from Dr. Chareinsuk Rojanaridpiched, Thai Tapioca Development Institute
Example of Impact from Thailand
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Three generations of improved Thai varieties increased starch content to 23% to 29%
Slides from Dr. Chareinsuk Rojanaridpiched, Thai Tapioca Development Institute
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Agronomic practices for research and farming successfully implemented
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IITA contributed 500 Anagyrus lopezi introduced from Benin to TTDI in Oct. 2009 for biocontrol of Phenacoccus manihoti. In Jan 2010 was first release to 600 ha. (tapiocathai.org)
IITA contribution to solve mealy bug threat to Thai Cassava Industry
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IITA contribution to development of cassava genomic resources
Cassava ultra-high density map• 532.5 Mb (~770Mb) draft
genome in 12977 scaffolds• Composite cassava map from
10 populations used to anchor the fragments to 18 pseudo-chromosomes
• Resulted in v5.1 reference genome!
• IITA contributed 9/10 of the populations
10 Populations; 22,403 SNPs
International Cassava Genetic Map Consortium,
Genes|Genomes|Genetics 5:133–144.
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Log
of o
dds
ratio
Rabbi et al. (2014) Crop Science 54
QTLs underlying key traits (TMS961089A x TMEB117)
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Major locus underlying carotenoid accumulation in cassava roots
Rabbi et al. Crop Science vol. 54:
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Multiple sequence alignment featuring C/T and A/C SNP polymorphism
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6128 clones genotyped and phenotypedWest Africa, East Africa~90K SNPs
GWAS supports narrow genetic base for major gene resistance to CMD
CMD2 Locus
(Wolfe et al. unpublished)
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Molecular markers for CBSD tolerance in three mapping populations
Above LOD 3 is significant1,000x more likely to be linked than independent assortment
5
Chromosome scale for a 20 MB chromosome
Var1, Site 1, Year 1 (LOD 3.92; 8.2%)Var1, Site 1, Year 2 (LOD 3.92; 8.2%)Var1, Site 2, Year 1 (LOD 5.07; 11.6%)
Mb 2010 15Mb 5
Var2, Site 1, Year 1 (LOD 10.75; 25.8%)Var2, Site 1, Year 2 (LOD 6.06; 21.4%)
Var2, Site 2, Year 1 (LOD 3.91; 8.6%)Var2, Site 2, Year 2 (LOD 3.62; 8.4%)
Var3, Site 1, Year 1 (LOD 3.56; 10.6%)
Var3, Site 2, Year 2 (LOD 4.33; 12.2%)
Tolerant parents are related: Namikonga (Kaleso), NDL06/132 and Nachinyaya
(Ferguson and students, unpublished)
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Resistance to cassava green mite
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Germplasm management
GRC core(256+24)TMEB(831+59)
669 duplicates
501 unique
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ADMIXTURE plots from ~4000 cassava accessions, including CIAT.
All samples (includes accessions with one or more clonal relationships were included in the analysis).
Below: Within individual inbreeding coefficient (F)
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Tracking of improved varieties in Ghana using DNA fingerprinting
Individual ancestry estimated
Hierarchical clustering
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Projection of identified clones on the Ghana map.
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Farmer-elicited variety names often do not match specific genotypes
182 different names recorded for the entire sample collection and here only names that occurred > 9 times are represented.
Meuwissen et al. 2001: “It was concluded that selection on genetic values predicted from markers could substantially increase the rate of genetic gain in animals and plants.”
GEBV = Genomic Expected Breeding Value = predicted quality as a parent
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Annual Breeding Cycle
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Popn. Trial Name
Locations # of plots Ubiaja Ibadan Ikenne MokwaLocal germplasm
1 agronomic traits Drought tolerance2 High dry matter3 Early Bulking4 breeders traits Selected CASTOR progenies5 Polyploids6 High flowering7 Male sterile source
8 disease/pest resistance White root CBSD resistance/tolerance9 CMD resistance
10 CGM resistance11 Hairy apical leaves (CGM biocontrol)12 Multiple pest resistance, yield and dry matter13 Genetic root necrosis14 Yellow root CBSD Population15 geographic location CIAT lines16 East African lines17 morphological traits Deep red color18 quality High HCN potential19 High carotenoids20 Poundability 21 Post harvest physiological deterioration22 High protein23 Yellow/white roots
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Challenge 1: Improve Starch Yield
• Break the dry matter content barrier to reliably produce starch of 23 to 29%.
• TMEB419 is the standard for high starch production in Nigeria and many countries with starch yield near 23%. Few have exceptionally high starch yield.
• Seasonal time of harvest is a key constraint to starch yield with best starch production in the dry season– Few year round harvest datasets to document
this
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Root weight vs Dry matter content
Ibadan Ikenne
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High Starch Genotypes in Advanced TestingHigh Starch set A – 7 locations, 2 years
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Partnership with FMARD and NRCRIHigh Starch set B, 7 locations, 2 years
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Challenge 2: Reaching needs of important beneficiaries
• Cassava breeding research must be gender responsive and address needs of all groups of farmers.
• Extension information reaches men easier than women
• 2015 Cassava Monitoring Study of 2500 households in Nigeria will be very helpful for guiding new strategies.
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Gender Differentiated Cassava Preferences
• Nextgeneration Cassava Breeding Project• Results from a pilot study in the four communities in SW and SE
Nigeria• Focus group discussions and individual interviews were held with
women, men and youth (women and men separately)• Results were analyzed using qualitative methods• Questions included:
– Characteristics of Respondents– The varieties grown– Traits preferred (implications to the Breeding program)– Access to resources– Marketing and Processing– Decision making
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Preferred Traits of Dangaria
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Preferred Traits of IITA
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Challenge 3: Meeting end user preferences
• Over 80% of cassava is consumed as food• While income can purchase nutrition security,
adoption of cassava varieties in many situations will depend on providing desired end user characteristics.
• Includes functional properties of cassava starches, leaf harvest, biofortification and time of harvest/in ground storage.
• Industrial traits will have specific target niches and will address specific markets. Do not assume one variety will meet all needs.
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Challenge 4: Breeding methods need to be accessible to all breeders in Africa
• Improved phenotyping methods for key traits• Field designs and analysis methods to maximize
heritability and address GxE• Successfully implement databases and secure
and accurate data collection• Determine the appropriate roles of genomic and
phenotypic selections with efficient use of appropriate markers.
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Cassavabase:A global open
access resource
All Data Available with Open Access with agreement to observe theToronto Protocol regarding use of unpublished data
• Mozambique: Colicanana, Nziva, Okhumelela, Orera and Eyope
• Zambia: Three varieties submitted for variety release to Seed Control and Certification Institute -SCCI)
• Biofortified clones introduced in evaluation in Malawi, Mozambique, Zambia and Swaziland.
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Cassava Breeding Achievements in East Africa
• Tanzania: with ARI released 8 varieties for the coastal lowlands of Tanzania in the past 5 years.
• Uganda: with NARO two breeding lines submitted for release as the first varieties with dual resistance to CMD (especially, EACMV-UG) and tolerance to both CBSD and UCBSD for the mid-altitude areas of East Africa bordering Lake Victoria
• Dozens of highly promising breeding lines in advanced stages of evaluation
• Under 5CP facilitating exchange and cooperative testing of 25 elite clones among 5 NARS breeding programs: Malawi, Mozambique, Kenya, Tanzania and Uganda.
• Botanical seeds from CBSD tolerant parents sent to Burundi (6,000), Rwanda (6,000) and DR Congo (10,000) to initiate national selection programs for specific adaptation to their agro-ecologies.
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Cassava Breeding Achievements in DR Congo
• With INERA released and registration of 21 improved varieties with harvest period from 12 to 20 months and root yield between 21 to 47 t/ha.
• New selection approach for cassava brown streak disease (CBSD) comprising evaluation of maturity time every 3 months and in ground storage to 24 MAP maintaining high starch yield
• Selection for new industrial traits including dietary fibre in leaves, amylose free starch for pasting industry and sugary cassava for ethanol
• In partnership with INERA and HarvestPlus new biofortified genotypes have been selected with total carotene content (TCC) up to 15µg/g fresh weight and more than the released check TMS-IBA01/1661 (9.5 µg/g)
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Building National Programs in Sierra Leone and Liberia
• Introduce and characterize local and advanced germplasm using morphological, biochemical and biotechnology tools
• Generate improved and adapted populations with desired characteristics for different agro-ecologies targeting different end uses (farming systems, food, feed and industry) and markets
• Establish special backup populations• Conserve a core set of local cultivars and improved germplasm • Fast track on farm evaluation through participatory evaluation using
innovation platforms• Expedite farmer access to quality planting materials on sustainable
basis• Improve NARs capacity and facilities (human, financial,
infrastructure) for effective execution of germplasm development, deployment and communication