1 Stress Monitoring in Plants via Chlorophyll Fluorescence and Plant Pigment Analysis MTV Workshop, 2020 11 March 2020, 11:05 am L. A. Finney, P. J. Skrodzki, M. Burger, J. Nees, and I. Jovanovic Department of Nuclear Engineering and Radiological Sciences, University of Michigan, 48109 Gerard Mourou Center for Ultrafast Optical Sciences, University of Michigan, 48109
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Stress Monitoring in Plants via Chlorophyll Fluorescence and Plant Pigment AnalysisMTV Workshop, 202011 March 2020, 11:05 am
L. A. Finney, P. J. Skrodzki, M. Burger, J. Nees, and I. JovanovicDepartment of Nuclear Engineering and Radiological Sciences, University of Michigan, 48109
Gerard Mourou Center for Ultrafast Optical Sciences, University of Michigan, 48109
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Team members and collaborators
MTV TeamUniversity of Michigan
L. A. Finney P. J. Skrodzki Dr. M. Burger Prof. I. Jovanovic
J. Nees
Van Vlack Laboratory for Instrumentation
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Mission relevance: remote, rapid detection of nonproliferation-relevant materials
Laser-based methods excite characteristic emissions that enable rapid, remote characterization of the makeup of any target.
Airborne laser scanning over extended distances may be used to track emissions and environmental contamination in order to detect undeclared nuclear activities such as uranium enrichment.
EPFL
S. Kemp, Sci. Glob. Sec. 16, 115 (2008)
DOE
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One potential method to monitor enrichmentactivities is through plants’ optical properties
Chlorophyll fluorescence imaging has been used extensively for large scale monitoring of CO2 uptake and circulation for climate change assessment.
We want to use laser excitation to understand plants’ response to other stresses, such as exposure to uranium in environments where solar-induced fluorescence is weak.
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The objective is to characterize spectral changesin response to stress for remote monitoring
MTV impact: National lab collaborations and internships
Collaboration:Dr. D. Weisz
Through CVT I did an internship at LLNL last summer as a Seaborg Institute summer intern.
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The Consortium for Monitoring, Technology, and Verification would like to thank the NNSA and DOE for the continued support of these research activities.
This work was funded by the Consortium for Monitoring, Technology, and Verification under Department of Energy National Nuclear Security Administration award number DE-NA0003920
Acknowledgements
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Backup slides
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Decay constant provide information about quenching and efficiency of different photosystems in the plant
Baker, Annu. Rev. Plant Biol., 113, 89(2008).
Buschmann, Photosyn. Res., 92, 261(2007).
PSII reaction center called P680 (primarily fluoresces at 680 nm)PSI reaction center called P735 (primarily fluoresces at 735 nm)
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Pigment ratios
Days after exposureexposure condition 1 2
ChlA:ChlB Chl:Car ChlA:ChlB Chl:CarControl
(watered) 7.23±0.18 3.14±0.01 2.68±0.13 4.93±0.15
Control (unwatered) 3.16±0.05 5.22±0.02 3.42±0.06 4.87±0.03