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• Congratulations for the bundle of
joy that is going to enter your life in
a matter of months.
www.CryobanksIndia.com
The Umbilical Cord
This is a structure that carries out the function of exchanging between a mother and a child.
Umbilical cord is a long “rope” like structure that connects baby with mother.
It consists of connective tissue, inside which there is an umbilical vein and two arteries.
Once thought to be a biological waste, has now been proven to have the potential to save millions of lives
from different life threatening diseases
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Why store Umbilical cord?
Part of Peri-natal tissue.
Most naive cells with high
regenerative potential.
Highest source of Hematopoietic
stem cells (HSC) & Hematopoietic
progenitor stem cells (HPCs).
The most convenient source of stem
cell retrieval.
No ethical concerns as cord is
discarded after delivery.www.CryobanksIndia.com
Umbilical Cord – The Ultimate Source Of
Master Cells
Umbilical Cord- Premiere source for stem cells by researchers and medical
fraternity :
Contains blood -Rich source for haemopoetic progenitor cells found in abundance.
The Wharton's jelly present in the inner lining of the cord is a rich source
for multipotent MSC’s found in abundance.
Painless extraction process .
Quality of stem cells extracted have higher potential compared to other adult
derived stem cells sources.
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Stem Cells & Types
The term “stem cells“ was proposed by a histologist A. Maksimov in 1908.
Every stem cell has two main characteristics:
• Self-renewable, which means that it can go through many cell division process and still remain in the same state.
• Potency, which means that it is capable of differentiating into various specific cell types.
Totipotent | Pluripotent | Multipotent | Unipotent
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Different Sources of Stem Cells
Embryonic Stem cells
For Research purpose only
Cord blood & Tissue
(HSC, HPC, MSCVSELs, Others)
Bone marrow stem cells for autologous & allogeneic use in
varied clinical applications
Peripheral Blood stem cells
(HSC, MSC)
Organs specific stem cells, Dental pulp, etc.
Adipose tissue derived stem cells for varied clinical
conditions
Others (minimally manipulated)
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Hematopoietic stem cells (HSCs)
Hematopoietic progenitor cells (HPCs)
Very small embryonic like stem cells (VSELs)
Endothelial colony forming cells (ECFCs)
Mesenchymal stem cells (MSCs)
Mixed resident cells
Wonder cells in Cord blood
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Wonder cells in Cord tissue
Mesenchymal stem cells (MSCs) (in
abundance)
Epithelial progenitor stem cells (EpSCs)
Mixed resident cells
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Cord blood banking & Beyond..
• Most primitive cell population with
high regenerative potential.
• Self-renewal capacity.
• Multi-potent – can differentiate into
various cell lineages.
• Immunosuppressant &
immunomodulatory effects.
• Minimal / Nil HLA representation
WJ – MSCs (Wharton’s jelly derived MSCs) possess an extensive proliferative potential and ability to differentiate into various cell types.
Key characteristics of MSCs:
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Steps in cord blood banking
1
The end product is stored at -196 deg. C for 21 years
The sample quality is evaluated and all the required tests are conducted
for maximum safety
2 3
456
After birth the umbilical cord is clamped and cut
Cord blood is collected from umbilical cord vein by experts
Collected cord blood is safely packed in proprietary transfer kits and within 24 hours reach
our labs
Cord blood is processed by patented technologies to yield maximum number of stem cells
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There is an enormous data suggesting the role
of stem cells in treatment of varied heart related ailments &
have shown excellent results in improving heart functions.
Stem cell therapy in Heart disease
• Acute Myocardial Infarction• Chronic Myocardial Infarction• Dilated Cardiomyopathy• Congenital defects
Heart diseases in final stage of clinical trials with SCT
Cord blood stem cells are useful in revascularization of heart tissue.MSCs differentiate into Cardiomyocytes and replace the dead heart muscles.
Advance storage of stem cells ensures ready availability of stem cells during medical urgencies.
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The multi-potent stem cells harvested from Wharton’s jelly of umbilical cord show Immuno-Modulatory effects and can largely reduce the required dosage of insulin
among Diabetic patients.
Stem cells play significant role in modulatingthe disease progress in diabetes patients.
The cord blood stem cells (HSCs) are in particular used therapeutically for treating varied stage diabetic Ulcers and chronic wounds.
Stem cell therapy in Diabetes
•Diabetes Mellitus type 1 & 2• Diabetic neuropathy• Diabetic retinopathy• Diabetic foot • Chronic non healing ulcers
Useful protocols of SCT in diabetic patients:
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Therapeutic ablation of bone marrow using CT
Replace with healthy CB / BM derived HSC + MSCs
Hematological disorders
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• Incidence of CP in India is 2-3 cases per 1000 live births• Brain injury during delivery is among the common causes of CP• Current estimate is >25 lakh children living with CP in the country.
Cerebral Palsy
SCT
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Other Applications of SCT
Osteoarthritis Autoimmune diseases Ophthalmic
Bone non-union A vascular necrosis Ischemic limb Stroke
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Cryobanks-Ultimate Solution
Services offered by Cryobanks International India:
• Collection of cord blood and tissue during delivery from locations across
the country.
• Transport of the cord blood and cord tissue to the processing centre in Gurgaon.
• Processing of cord blood to generate haemopoetic stem cells and
haemopoetic progenitor cells via automated and semi-automated process.
Processing of cord tissue to generate multipotent MSC’s which are ready to use and delivered along with proper characterization, differentiation and endotoxin and mycoplasma analysis abiding to the ISCT guidelines( global norms)
Cryogenic preservation of cord blood and cord tissue stem cells.www.CryobanksIndia.com
• Cell processing and cryo-preservation.• Cord cell lines.• Cell culture and Expansion.• Cell differentiation.• Bone marrow processing.• Varied Stem cell & Regenerative medicine
related clinical applications.• Customized therapy solutions.
Our Facility Services
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Cryobanks - At a glance .
A JV between Cryobanks International USA (presently Lifeforce cryobanks)and RJ
Corp founded in 2006.
A leader in the collection, processing and banking of umbilical cord blood stem
cells.
Provider of Private Cord Blood Storage Program for expecting families
Quality standard’s approved by USFDA ,AABB and DCGI
Reviva- Independent wing for MSC extraction in association with Da Vinci
biosciences.
International presence in Thailand, Africa, Gulf, Bangladesh and Nepal.
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Food Beverages Medical Education
OTHERS
Modern Montessori
School
Business Verticals
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Our coverageAsia
Nepal, Bangladesh,
Srilanka, Myanmar,
Laos, Vietnam,
Cambodia, Uzbekistan
Middle EastU.A.E, Oman, Qatar,
Bahrain, Kuwait
AfricaSouth Sudan, Ethiopia,
Uganda, Rwanda,
Tanzania, Malawi,
Mozambique, Zambia
Starting in 2012Asia
Malaysia, Philippines,
Indonesia, Singapore
AfricaSouth Africa, Nigeria
Our Storage Facilities:
INDIA (Gurgaon)THAILAND (Bangkok)
KENYA (Nairobi)
We serve in many parts of the world- RJ Health
care ( Cord blood banking)
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Journey of RJ Healthcare
Started in 2006 with cord blood processing technology and cord preservation
2007-Expansion of operations across the country
2008 –Expanded beyond borders as in UAE & Nepal
2009 - obtained the development of technology –SEPAX
2010 - collaboration with Da Vinci Biosciences to start up REVIVA
2010 – expansion of operations to Thailand –CRYO-VIVA
2010 – started operations in the field of Oral Stem cell rejuvenation formulations in the name of Aqua stem life.
2011 - operations of REVIVA labs processing cord tissue cells to extract MSC’s using the patented technology of EXPLANT
2011- we expanded our business horizons to cover Bangladesh
2012- launched our projects : Africare (Africa), diagnostics , and birthing center-COCOON.
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“Collaboration with BIOSAFE, a fully automated FDA approved technology for Bone marrow
processing for different clinical applications.”
“Patented technology (EXPLANT METHOD) from Da Vinci Biosciences, USA with approved & clinical
Grade media & reagents for maximum safety.”
Only Lab in the country to have International collaborations & technology exchange for cord tissue derived MSC culture.
Our Technology Partners
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Technology Milestones- Sepax
• Sepax® -An automated stem cell processing technology, a safe, sterile and fully
automated cord blood processing technology – so you get more stem cells for
maximum transplant success.
• The only fully automated available system for cord blood stem cell isolation
That claims to have processed over 2,000 cord blood units subsequently used in
transplantation
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Advantages of Sepax
Features of SEPAX technology Benefits to You
Swiss-made patented automated cord blood processing system
Availability of the best of technology for the processing of your babies sample
Requires no operator intervention during processing
There is constant result for all sample which is standard being automated and sensor basedAllows faster cellular processing
Able to achieve as high as 99%¹ Total Nucleated Cells (TNC) recovery rate
higher recovery of cells giving better acceptability of the sample during therapy as SEPAX processed sample result in higher cell count extraction.
Closed processing system highly sterile process
Process every cord blood unit individually
There are no chance of mix up of samples as the sample gets processed as a individual sample till the processing is completed and the stem cells are totally extracted.
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Cleared by U.S. FDA, approved by European Union (evidenced by CE Mark), and Japan MHLW (Ministry of Health and Labor Welfare)
Abides to the international standards of processing.
Complies with the highest quality standards such as AABB & FACT-Netcord³
An advanced cord blood processing technology with proven transplant track record
Proven and validated technology with reliable results across the globe for which the technology has immense acclamations.
World’s most tried and reliable automated system
Processed more than 500,000 units³ in over 38 countries worldwide
Trusted by majority of the world’s leading public and private cord blood banks
Over 70% of Netcord banks (public banks in Europe) use Sepax® for cell processing
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Explant
Benefits of Explant processing over conventional enzymatic process
:
Method Cell Count Cell Viability Cell Potency
REVIVA EXPLANTMETHOD
Akin to natural cell proliferation
Higher yield per cm of Umbilical Cord
Better viabilityCells with highest
regeneration potential proliferate
ENZYMATIC METHODChemicals used –artificial process
5 to 10 times lower yield
Viability compromised as cell
surface damage is possible
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• Time proven and highly recommendable technology.
• Patented clinical grade reagents.
• Higher cell viability and sustaining all the characteristics of stem cells.
• Efficient cell identification & differentiation into different cell lines.
• Maximum safety & therapeutic value.
Reviva cell recommends the proprietary “EXPLANT TECHNIQUE”for maximum safety
Explant Technology
The time proven technology has made it possible to store millions of multi-potent cells in advance for in-
future medical urgencieswww.CryobanksIndia.com
Our lab facility
• Over 28000 sq ft dedicated laboratory.
• cGMP, cGTP, cGLP compliant labs.
• State-of-the-art cell culture & expansion facility with patented technology in collaboration with DA Vinci Biosciences, USA.
• Cord blood sample processing on USFDA approved fully automated platform.
• Class 10,000 & class 100 clean rooms.
• Comply to National & International accreditations.
• Highly qualified and dedicated staff.www.CryobanksIndia.com
Cord blood Processing Lab
• USFDA approved fully automated SEPAX technology
• High yield of MNC & CD34+ stem cells essential for ensuring the cord blood unit has enough cells for in future therapeutic application.
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Global Standards
1) AABB accredited.
2) NABL accredited
3) DGCI approved
4) ISO certified
5) Business Biz
6) Leaders of tomorrow
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Our Stringent Protocol
• Only cord blood bank in the country which uses a double check process for the entire
processing of the cord blood as under:
• Environmental testing of processing lab ( normal management by HEPA filters)
• Swab culture
• Petri plate culture
• Sterility testing
• Gram staining
• Bact Alert automated detection
• Viability testing
• Coulter
• Facs caliber
• Maternal blood testing:
• ELISA process
• NAT testing
• Preservation process:
• Cryobags made of EVA
• LN2 overwrap on the EVA bags for further protection.
• DGCI approved public bank enabling higher quality control mechanism.www.CryobanksIndia.com
OUR OFFERINGS Free Add on sample
Cash Back offer
CRYO SAFETY :
5 lakhs transplant assistance
10 lakhs security and quality commitment
Free world wide shipment
Guarantee of sample during transport to facility
Processed MSC’s ready to use ( number dependent on plan chosen)
www.CryobanksIndia.com
Strong Financial Background.
Diversified Healthcare segments
comprising of both forward and
reverse integration of business.
Worldwide existence and technical
collaboration.
Independent association for
Technologies related to cord blood
processing ( Lifeforce Cryobanks) and
cord tissue processing( Da Vinci
Biosciences) .
Processing of cord tissue using
PATENTED EXPLANT technology.
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• Provision of MSC’s ready to use
along with all pretherapy analysis
• SEPAX technology for cord blood
processing
• Double check process for ensuring
safety of sample preserved
• Public banking operations in India
approved by DCGI
• Cash Back offer
• Free Medical guidance during
therapy requirements.
www.CryobanksIndia.com
Customer Relationship Management• Continuous interaction and updates to clients on
advancements in the field of stem cell therapies and organizational aspects.
• Dedicated 24X7 toll free assistance
• Dedicated mail for any kind of requirements of clients – [email protected]
• Dedicated club for existing customers with special loyalty program in form of Cryoclub which can be reached at [email protected].
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Our Service Standards in India
Cryobanks- Cord Blood Retrievals
Therapy details Disease TreatedDoctor who did the therapy Remarks
CB+ BM Therapy6/6 HLA Match of Sibling Thalessimia Dr. Dharma Choudhary Cured Declared
CB therapy used for Analogous Transfer 4/6 match Thalassemia Dr. Revathi Cured Declared
CB + BM therapy used for analogous transfer 4/6 match Thalassemia Dr. Revathi Cured Declared
CB therapy used for sibling5/6 match Cerebral Palsy Dr. Anupam Sachdev
Under observation perfromed in Jan,2011-Patient shows recovery post therapy and would be officially declared within 2 months
CB therapy used for autologoustransplant Thalassemia Dr. Arun last sitting pending
CB therapy used for sibling6/6 match Cerebral Palsy Dr. Ranade
received stem cell therapy after the expansion of MNC at NICHI centre in Chennai. The child had improvement in motor functions after one month
NOTE: Other than these therapies there are 4other thalassemia cases that are done and the patient is under observation so we have not declared that same yet as the gestation period is one year.
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Cryobanks- MSC’s retrieval
RETRIEVALS OF CORD TISSUE
Month of retrieval Therapy details Disease TreatedDoctor who did the therapy REMARKS
Nov-11MSC's of preserved tissue fpr same child Cerebral palsy Dr. B. S Rajput
considerable development , results to be declared
Dec, 2012
MSC's of preserved tissue for grand mother Spinal cord injury Dr. B. S Rajput
Development in the patient and movement possible
Feb, 2012MSC's of preserved tissue for sibling Diabetes Dr. Anant Bagul Therapy continuing
April, 2012MSC's of preserved tissue for same child Autism Dr. Himanshu
Two sittings done , 4 pending
Sept, 2012MSC's of the preserved tissue for same child Cerebral Palsy Dr. B. S Rajput
first sitting scheduled on 4th oct, 2012 and therapy to continue till march 2013 in multiple sittings
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Our Medical Board
• Dr. Naresh Trehan
• Dr. Mrinalini Chaturvedi
• Dr. Phagun Shah
• Dr. Anubhav Pandey
• Dr. Sonal Sharma
• Dr. Ashish Munjal
• Dr. Vikas Verma
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Clinical conditions supported by Cryobanks
Disease Source of stem cells
Peripheral vascular disease Autologous Bone marrow
Myocardial Infarction Autologous Bone marrow / Cryopreserved stem cells
Cerebral palsy Autologous Bone marrow/ Cryopreserved stem cells
Thalassemia Cryopreserved stem cells
Diabetes Mellitus type-1 / 2 Autologous Bone marrow/ Cryopreserved stem cells
Spinal cord injury Autologous Bone marrow/ Cryopreserved stem cells
Aplastic anaemia Cryopreserved stem cells
Leukemia Cryopreserved stem cells
Autism Autologous Bone marrow/ Cryopreserved stem cells
Diabetic foot / Ulcers Autologous Bone marrowwww.CryobanksIndia.com
Stem cell therapy centers- India
Sl No. Hospital Name City
1Nichi-In Centre for Regenerative Medicine Chennai
2 Sanjay Gandhi PGIMS Lucknow
3 Tata Memorial Hospital Mumbai
4 Inlaks Budhrani Hospital Pune
5 Armed Forces Medical College Pune
6 Army R & R Hospital Delhi
7 Christian Medical College Vellore
8Narayana Hrudayalaya Health City Bangalore
9 Manipal Hospital Bangalore
10Trinity Hospital & Heart Foundation Bangalore
11 Global Hospital Hyderabad
12 NIMS Hospital Hyderabad
13 TRICell Therapeutics Pvt. Ltd. Chennai
14 Adyar Cancer Institute Chennai
15 Apollo hospital Gandhinagarwww.CryobanksIndia.com
Stem cell therapy centers- India
16 Apollo Hospital Chennai Chennai
17 Apollo Specialty Hospital Chennai
18All India Institute of Medical Science (AIIMS) Delhi
19 Apollo Gleneagles Hospital kolkata
20 Fortis Vasant Kunj Noida
21 Fortis Hospital Noida Delhi
22 Moolchand Medcity Delhi
23 Adiva Delhi
24International Stem Cell Services Limited Bangalore
25 StemOne Biologicals Pvt. Ltd. Pune
26Chaitanya Stem Therapy Center Pune
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With you always..
Invest for life..!!Gift your child the power of self healing
Contact us to know more about stem cell banking:1-800-180-1217 (BSNL) I 1-800-102-2796 (AIRTEL) I +91-9582-2844-44
Head office:129, Pace City – 1, Sector- 37, Gurgaon -122001, Haryana, [email protected] I www.cryobanksindia.com
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