23.5.2013 1 LIGNOCELLVALUE-ADDED MATERIALS AND FUNCTIONAL STRUCTURES FROM LIGNOCELLULOSICS Steering Committee Meeting / 24.5.2013 Scientific Report (see also budget info at the end) http://www4.ncsu.edu/~ojrojas/Lignocell.htm Lignocell: Instrument to develop knowledge in lignocellulose science and engineering Students: • Temporal: Learn from core competences and apply their skills in proposed Lignocell subjects • Permanent: Long-term learning to become top-notch scientists Mentors: To provide ideas, guidance and to connect people Industry: Opportunity to “steer” work in strategic areas in an open, scientifically-driven effort 2
83
Embed
Steering Committee Meeting / 24.5 - Nc State Universityojrojas/Lignocell/Report May 2013.pdf · 23.5.2013 2 Laura Taajamaa Aalto, FIN Dr. Arcot Lokanathan Aalto, FIN o - y Dr. Cristina
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
23.5.2013
1
LIGNOCELLVALUE-ADDED MATERIALS AND FUNCTIONAL STRUCTURES FROM LIGNOCELLULOSICS
Steering Committee Meeting / 24.5.2013
Scientific Report
(see also budget info at the end)
http://www4.ncsu.edu/~ojrojas/Lignocell.htm
Lignocell: Instrument to develop knowledge in lignocellulose science and engineering
Students: • Temporal: Learn from core competences and apply
their skills in proposed Lignocell subjects• Permanent: Long-term learning to become top-notch
scientists
Mentors:To provide ideas, guidance and to connect people
Industry: Opportunity to “steer” work in strategic areas in an open, scientifically-driven effort
(Raquel Martin / OR)3. Hydrolysis of SEW nanofibers & BC
(Luis Morales)4. Novel methods in NFC production
(Carlos Carrillo / OR)5. SEW fibers, NFC and nanopaper
(Ester Rojo)6. NFC aerogels with SPs
(Julio Arboleda/OR)7. NFLC aerogels
(Mariko Ago/OR)8. Laccase-mediated coupling
(Oriol Cusola / OR)9. Carbon nanodots
(Kaoliina Junka / OR)10. CNC modeling
(Henry Bock)11. Surface chemistries
(Ilari Filpponen)12. Asymmetric CNC modification
(Arcot Lokanathan)Laura Taajamaa in Maternity Leave
23.5.2013
4
Laura Taajamaa in Maternity Leave
Subjects1. Introduction and general report
(Orlando Rojas)2. Hydrolysis of bicomponent films
(Raquel Martin / OR)3. Hydrolysis of SEW nanofibers & BC
(Luis Morales)4. Novel methods in NFC production
(Carlos Carrillo / OR)5. SEW fibers, NFC and nanopaper
(Ester Rojo)6. NFC aerogels with SPs
(Julio Arboleda/OR)7. NFLC aerogels
(Mariko Ago/OR)8. Laccase-mediated coupling
(Oriol Cusola / OR)9. Carbon nanodots
(Kaoliina Junka / OR)10. CNC modeling
(Henry Bock)11. Surface chemistries
(Ilari Filpponen)12. Asymmetric CNC modification
(Arcot Lokanathan)
23.5.2013
5
1. Hoeger, I.C., Gleisner, R., Negron, J., Rojas, O.J., Zhu, J.Y., Bark Beetle-killed Lodgepole Pine for the Production of Submicron Lignocellulose Fibrils, Accepted Journal Forest Science (2013)
2. Zhang, Y., Nypelö, T., Salas, C., Arboleda, J., Hoeger, I., Rojas, O.J.CelluloseNanofibrils: From Strong Materials to Bioactive Surfaces, Accepted Journal of Renewable Resources (2013)
3. Goli, K., Gera, N. Liu, X., Rao, B., Rojas, O.J., Genzer, J., Generation and properties of antibacterial coatings based on electrostatic attachment of silver nanoparticles to protein-coated polypropylene fiber, Accepted ACS Applied Materials & Interfaces, (2013)
4. Garcia-Ubasart, J., Vidal, T., Torres, A.L., Rojas, O.J. Laccase-mediated coupling of nonpolar chains for the hydrophobization of lignocellulose, Biomacromolecules, Accepted DOI: 10.1021/bm400291s (2013)
5. Song, J., Rojas, O.J., Approaching Superhydrophobicity Based on cellulosic materials: A Review, Nordic P&P Research Journal, Accepted (2013)
6. Hubbe, M.A., Rojas, O.J., Fingas, M., Gupta, B.S.,Cellulosic Substrates for Removal of Pollutants from Aqueous Systems: A Review. 3. Spilled Oil and Emulsified Organic Liquids, Bioresources , 8(2): 3038-3097 (2013)
7. Martín-Sampedro, R., Rahikainen, J.L., Johansson, L-S., Marjamaa, K., Laine, J., Kruus, K., Rojas, O.J., Preferential adsorption and activity of monocomponent cellulases on lignocellulose thin films with varying lignin content, Biomacromolecules, 14: 1231–1239 (2013)
9
8. Taajamaa, L., Rojas, O.J., Laine, J., Yliniemi, K., Kontturi, E. Protein-assisted 2D assembly of gold nanoparticles on a polysaccharide surface, Chemical Communications, 59: 1318-1320 (2013).
9. Ago, M., Jakes, J., Rojas, O.J. Thermo-Mechanical Properties of Lignin-based Electrospun Nanofibers and Films Reinforced with Cellulose Nanocrystals, Biomacromolecules, accepted
10. Zhang, Y., Islam, N., Carbonell, R.G., Rojas, O.J. Specific binding and detection of IgGby bioactive short peptides immobilized on supported copolymer layers, Analytical Chemistry, 2013, 85 (2): 1106–1113 (2013).
11. Salas, C.; Rojas, O.J.; Lucia, L.A.; Hubbe, M.A., Genzer, J., On the surface interactions of proteins with lignin, ACS Applied Materials & Interfaces, 5: 199-206 (2013)
12. Rahikainena, J., Martin-Sampedro, R., Heikkinena, H., Rovioa, S., Marjamaaa, K., Tamminena, T., Rojas, O.J., Kruus, K., Inhibitory effect of lignin during cellulose bioconversion: the effect of lignin chemistry on non-productive enzyme adsorption, Bioresource Technology, 133, 270–278 (2013)
13. Hoeger, I.C., Nair, S.S., Ragauskas, A.J., Yulin Deng, Y., Rojas,O.J., Zhu, J.Y., Mechanical Deconstruction of Lignocellulose Cell Walls and their Enzymatic Saccharification, Cellulose, 20: 807-818 (2013).
14. Junka, K., Filpponen, I., Johansson, L-S., Kontturi, E., Rojas, O.J., Laine, J., A method for the heterogeneous modification of nanofibrillar cellulose in aqueous media, Carbohydrate Polymers, Accepted doi:10.1016/j.carbpol.2012.11.063.
15. Park, J., Meng, J., Lim, K.H., Rojas, O.J., Park, S. Transformation of lignocellulosic biomass during torrefaction, Journal of Analytical and Applied Pyrolysis, 100: 199–206(2013). 10
17. Martin-Sampedro, R., Filpponen, I.; Hoeger, I.C., Zhu, J.Y., Laine, J., Rojas, O.J., Rapid and Complete Enzyme Hydrolysis of Lignocellulosic Nanofibrils, ACS Macro Letters, 1, 1321-1325 (2012)
18. Goli, K, Rojas, O.J., Genzer, J. Formation and antifouling properties of amphiphiliccoatings on polypropylene fibers, Biomacromolecules, 13, 3769-3779 (2012).
19. Orelma, H., Filpponen, I., Johansson, L-S., Österberg, M., Rojas, O.J., Laine, J. Surface functionalized nanofibrillar cellulose (NFC) film as a platform for rapid immunoassays and diagnostics, Biointerphases, 7, 61 (2012).
20. Hoeger, I.C., Filpponen, I., Martin-Sampedro, R., Johansson, L-S., Österberg, M., Laine, J., Kelley, S., Rojas, O.J. Bi-component lignocellulose thin films to study the role of surface lignin in cellulolytic reactions, Biomacromolecules, 13, 3228–3240 (2012).
21. Ago, M., Jakes, J.E., Johansson, L-S., Park, S., Rojas, O.J. Interfacial Properties of Lignin-based Electrospun Nanofibers and Films Reinforced with Cellulose Nanocrystals, ACS Applied Materials and Interfaces, 4(12): 6849-6856 (2012).
22. Hao-yu, J., Lucia, L.A., Rojas, O.J., Hubbe, M.A., Pawlak, J.J., A Survey of Soy Protein Flour as a Novel Dry Strength Additive for Papermaking Furnishes, Journal of Agricultural and Food Chemistry, 60, 9828-33
11
23. Ferrer, A., Filpponen, I., Rodríguez, A., Laine, J., Rojas, O.J. Valorization of Residual Empty Palm Fruit Bunch Fibers (EPFBF) by Microfluidization: Production of Nanofibrillated Cellulose and EPFBF Nanopaper, Bioresource Technology, 125, 249-255 (2012).
24. Ferrer, A., Quintana, E., Filpponen, I., Solala, I., Vidal, V., Rodríguez, R., Laine, J., Rojas, O.J. Effect of Residual Lignin and Heteropolysaccharides in Nanofibrillar Cellulose and Nanopaper, Cellulose, 19, 2179–2193 (2012)
25. Orelma, H., Johansson, L-S., Filpponen, I., Rojas, O.J., Laine, J. Generic Method for Attaching Biomolecules via Avidin-Biotin Complexes Immobilized on Films of Regenerated and Nanofibrillar Cellulose, Biomacromolecules, 13, 2802−2810 (2012)
26. Carrillo,C.A., Saloni, D., Lucia, L.A., Hubbe, M.A., Rojas, O.J. Capillary flooding of wood with microemulsions from Winsor I systems, Journal of Colloids and Interface Science, 381, 171–179 (2012).
28. Payne, K., Jackson, C., Aizpurua Gonzalez, C., Rojas, O.J., Hubbe, M., Oil Spills Abatement: Factors Affecting Oil Uptake by Cellulosic Fibers, Environmental Science & Technology, 46:7725-7730 (2012)
29. Vallejos, M.E., Peresin, M.S., Rojas, O.J. All-Cellulose Composite Fibers Obtained by Electrospinning Dispersions of Cellulose Acetate and Cellulose Nanocrystals, Journal of Polymers and the Environment, 20:1075–1083 (2012).
12
23.5.2013
7
1. Taajamaa, L., Laine, J., Kontturi. E., Rojas, O.J., Bicomponent fibre mats with adhesive ultra-hydrophobicity tailored with cellulose derivatives J. Mater. Chem., DOI:10.1039/C2JM30572K.
2. Zoppe, J.O., Venditti, R.A., Rojas, O.J. Pickering emulsions stabilized by cellulose nanocrystals grafted with thermo-responsive polymer brushes. Journal of Colloid and Interface Science, 369 202–209 (2012)
3. Goli, K., Rojas, O. J., Ozcam, A., Genzer, J. Generation of functional coatings on hydrophobic surfaces through deposition of denatured proteins followed by grafting from polymerization, Biomacromolecules, In press, DOI: 10.1021/bm300075u
4. Castro, C., Zuluaga, R., Álvarez, C., Putaux, J-L., Caro, G., Rojas, O.J. Mondragon, I., Gañán, P. Bacterial cellulose produced by a novel acid-resistant strain Gluconacetobacter medellensis, Carbohydrate Polymers, In press, DOI: 10.1016/j.carbpol.2012.03.045
5. Ago, M., Okajima, K., Jakes, J.E., Park, S., Rojas, O.J., Lignin-based biomimetic electrospun nanofibers reinforced with cellulose nanocrystals, Biomacromolecules, 13: 918–926 (2012)
6. Salas, Carlos, Rojas, O. J., Lucia, L. Hubbe, M.A., Genzer, J. Adsorption of glycinin and ß-conglycinin on silica and cellulose:surface interactions as a function of denaturation, pH, and electrolytes, Biomacromolecules, 13: 387-396 (2012)
7. Li, Y., Rojas, O.J., Hinestroza, J.P., Boundary Lubrication of PEO-PPO-PEO Triblock Copolymer Physisorbed on Polypropylene, Polyethylene, and Cellulose Surfaces, Ind. Eng. Chem. Res. , 51: 2931-2940 (2012)
8. Liu, X., He, F., Salas, C., Pasquinelli, M., Genzer, J., Rojas, O.J. Experimental and Computational Study of the Effect of Alcohols on the Solution and Adsorption Properties of a Nonionic Symmetric Triblock Copolymer, Journal of Physical Chemistry B, 116: 1289–1298 (2012).
9. Liu, H., Li, Y., Krause, W., Rojas, O.J., Pasquinelli, M. The Soft-Confined Method for Creating Molecular Models Amorphous Polymer Surfaces, The Journal of Physical Chemistry B, 116: 1570–1578 (2012)
10. Li, Y., Rojas, O.J., Hinestroza, J.P., Boundary Lubrication of PEO-PPO-PEO Tri-block Copolymer Physisorbed on Polypropylene, Polyethylene and Cellulose surfaces, Industrial & Engineering Chemistry Research
11. Liu, H., Li, Y., Krause, W., Pasquinelli, M., Rojas, O.J. Mesoscopic Simulations of the Phase Behavior of Aqueous EO19PO29EO19 Solutions Confined and Sheared by Hydrophobic and Hydrophilic Surfaces, ACS Applied Materials & Interfaces, 4: 87-95(2012)
12. Orelma, O., Filpponen, I., Johansson, L-S, Laine, J., Rojas, O.J. Modification of Cellulose Films by Adsorption of CMC and Chitosan for Controlled Attachment of Biomolecules Biomacromolecules, 12(12): 4311–4318(2011).
13. Taajamaa, L., Rojas, O.J., Laine, J, Kontturi. E. Phase-specific pore growth in ultrathin bicomponent films from cellulose-based polysaccharides, Soft Matter, 7: 10386-10394 (2011)
13
14. Hoeger, I., Rojas, O.J., Efimenko, K., Velev, O.D., Kelley, S.S. Ultrathin film coatings of aligned cellulose nanocrystals from a convective-shear assembly system and their surface mechanical properties Soft Matter, 7 (5), 1957-1967 (2011)
15. Csoka, L., Hoeger, I., Peralta, P., Peszlen, I., Rojas, O.J. Dielectrophoresis of cellulose nanocrystals and their alignment in ultrathin films by electric field-assisted shear assembly, Journal of Colloid and Interface Science, 363(1):206-12 (2011).
17. Zoppe, J.O., Österberg, M., Venditti, R.A., Laine, J., Rojas, O.J. Surface Interaction Forces of Cellulose Nanocrystals Grafted with Thermo-responsive Polymer Brushes, Biomacromolecules, 12 (7): 2788–2796 (2011).
18. Liu, X., Vesterinen A-H., Genzer, J., Seppälä, J.V., Rojas, O.J. Adsorption of PEO−PPO−PEO Triblock Copolymers with End-Capped Cationic Chains of Poly(2-dimethylaminoethyl methacrylate), Langmuir, 27 (16), 9769–9780 (2011).
19. Martin-Sampedro, R., Capanema, E.A., Hoeger, I., Villar, J.C., Rojas, O.J. Lignin Changes after Steam Explosion and Laccase-Mediator Treatment of Eucalyptus Wood Chips, Journal of Agricultural and Food Chemistry, 59 (16): 8761–8769 (2011).
20. Li, Y., Liu, H., Song, J., Rojas, O.J., Hinestroza, J.P., Adsorption and Association of a Symmetric PEO-PPO-PEO Triblock Copolymer on Polypropylene, Polyethylene, and Cellulose Surfaces, ACS Applied Materials and Interfaces, 3 (7): 2349–2357 (2011)
21. Wu, N., Hubbe, M.A., Rojas, O.J., Park, S., Permeation of a Cationic Polyelectrolyte into Meso-porous Silica. Part 3, Colloids and Surfaces A, 381, 1-6 (2011).
22. Liu, X., Kiran, K., Genzer, J., Rojas, O.J. Multilayers of Weak Polyelectrolytes of Low and High Molecular Mass Assembled on Polypropylene and Self-assembled Hydrophobic Surfaces, Langmuir 27 (8), 4541–4550 (2011)
23. Spence, K.L., Venditti, R.A., Rojas, O.J., Habibi, Y., Pawlak, J.P. A comparative study of energy consumption and physical properties of microfibrillated cellulose produced by different processing methods, Cellulose, 18:1097–1111 (2011).
24. Wang, Z., Hauser, P., Rojas, O.J., Multilayers of low-charge-density polyelectrolytes on thin films of carboxymethylated and cationic cellulose, Journal of Adhesion Science and Technology, 25 (6-7), 643-660 (2011)
25. Álvarez, C., Rojano, B., Almaza, O.,Rojas, O.J., Gañán, P., Self-bonding boards from plantain fiber bundles after enzymatic treatment, Journal of Polymers and the Environment, 19(1), 182-188 (2011).
26. Silva, D.J., Rojas, O.J., Hubbe, M.A., Park, S.W. Enzymatic treatment as a pre-step to remove cellulose films in from sensors, Macromolecular Symposia, 299/300, 107–112 (2011). 14
23.5.2013
8
1. Rojas, O.J., Nanoparticles and Nanostructures from Direct- and Self- Assembly of Components Cleaved from Fiber Cell
Walls, XXI International Materials Research Congress (MRS), Cancun, Mexico, August 12-16, 2012
2. Rojas, O.J., Nypelo, T., Ago, M., Zhang, Y., Taajamaa, L., Orelma, H., Filpponen, I. Laine, J. Cellulose as Tunable
Material in Nanotechnologies: Thin Films of Cellulose and Cellulose Derivatives with Designed Properties by Surface
Modification, 3rd International Cellulose Conference, Sapporo, Japan, October 10-12, 2012.
3. Filpponen, I., Lokanathan, A., Rojas, O.J., Laine, J. Click chemistry reactions on the reducing end groups of cellulose
nanocrystals, 3rd International Cellulose Conference, Sapporo, Japan, October 10-12, 2012.
4. Martín-Sampedro, R., Rahikainen, J., Hoeger, I., Marjamaa, K., Kruus, K., Filponnen, I., Laine, J., Rojas, O.J., 4th Effects
of Lignin on the Hydrolysis of Cellulose by Pure and Multicomponent Enzymes, International Conference on Pulping,
Papermaking and Biotechnology (ICPPB’12), Nanjing, China, November 7-9, 2012
5. Ago, M., Silveira, J., Taajamaa, L., Jakes, J.E., Kontturi, K., Bittencourt, E., Laine, J., Rojas, O.J., Electrospun Micro-
and Nano- Fibers from Multicomponent Lignocellulose Systems: Functional Materials with Special Surface, Mechanical
and Thermal Properties, International Conference on Pulping, Papermaking and Biotechnology (ICPPB’12), Nanjing,
China, November 7-9, 2012
6. Hubbe, M.A., Payne, K.C., Jackson, C.D., Aizpurua, C.E., Rojas, O.J. Application of Cellulosic Fiber Materials for The
Remediation of Petroleum Spills in Water, International Conference on Pulping, Papermaking and Biotechnology
(ICPPB’12), Nanjing, China, November 7-9, 2012.
7. Filpponen, I., Laine, J., Rojas, O.J. Click chemistry for producing lignin-based novel materials, International Conference
on Pulping, Papermaking and Biotechnology (ICPPB’12), Nanjing, China, November 7-9, 2012.
8. Carrillo, C., Rojas, O.J. High water content microemulsions as a novel method for wood pretreatment and extraction,
12th European Workshop on Lignocellulosics and Pulp, Espoo, Finland, August 27-30, 2012.
9. Silveira, J.V.W., Millas, A.L.G., Tessarolli, L.F., Ago, M., Rojas, O.J., Bittencourt, E., Produção de Fibras Eletrofiadas a
Partir de Acetato de Celulose e Lignina, XIX Brazilian Congress in Chemical Engineering (COBEQ 2012), Búzios, RJ,
Brazil, September 9-12, 2012
16
23.5.2013
9
245th ACS Meeting, April 7-11, 2013 | New Orleans, Louisiana
1. In situ self-assembly and hydrophobization of Gluconacetobacter bacterial cellulose,Cristina Castro, Robin Zuluaga,
2. Short peptide-conjugated copolymer based biosensor for specific binding of immunoglobulin G, Yanxia Zhang, Orlando
Rojas, Nafisa Islam, Ruben Carbonell
3. Lignin nano- and microparticles for coating and interfacial stabilization, Tiina Nypelo, Mariko Ago, Shuai Li, Orlando
Rojas
4. Effect of composition and formulation variables in biomass flooding capacity by o/w microemulsions, Carlos A Carrillo,
Daniel Saloni, Orlando J Rojas
5. Phase behavior and properties of the oil-in-water emulsions stabilized by carboxymethylated and acetylated lignins,
Shuai Li, Maryam Mazloumpour, Professor Julie Willoughby, Professor Orlando J Rojas
6. Magnetic cellulose nanocrystals: Demonstration and properties of organic-inorganic hybrid system, Tiina Nypelo, Carlos
Rodriguez-Abreu, José Rivas, Michael Dickey, Orlando Rojas
7. Surface modification of hydrophobic substrates by soy protein adsorption, Carlos L. Salas, Orlando J. Rojas, Jan
Genzer, Martin A. Hubbe, Lucian Lucia
8. Cellulose acetate/lignin-based electrospun fibers, Joao V. W. Silveira, Ana L. G. Millas, Mariko Ago, Orlando J. Rojas,
Edison Bittencourt
9. Mechanical deconstruction of lignocellulose cell walls and production of nanopaper, Ingrid C Hoeger, Orlando J Rojas,
Junyong-FS Zhu
10.Effects of lignin and hemicelluloses on the enzymatic hydrolysis of nanofibrillated softwood lignocellulose after SO2-
ethanol-water (SEW) fractionation, Luis O Morales, Mikhail Iakovlev, Jenni Rahikainen, Leena-Sisko Johansson, Raquel
Martin, Janne Laine, Adriaan van Heiningen, Orlando Rojas
17
245th ACS Meeting, April 7-11, 2013 | New Orleans, Louisiana
12. Influence of the deconstruction of the cell wall in the enzymatic saccharification of softwoods, Ingrid C Hoeger, Sandeep
S Nair, Professor Arthur J Ragauskas, Professor Yulin Deng, Professor Orlando J Rojas, Junyong-FS Zhu
13.Asymmetric thiolation of cellulose nanocrystals using reductive amination of reducing ends, Lokanathan R Arcot, Jani
Seitsonen, Antti Nykänen, Leena S Johansson, Joseph Campbell, Janne Ruokolainen, Olli Ikkala, Orlando Rojas, Janne
Laine
14.Protein-assisted 2D assembly of gold nanoparticles on an ultrathin cellulose film, Laura Taajamaa, Orlando J Rojas,
Janne Laine, Eero Kontturi
15.Synthesis and characterization of soy protein-nanocellulose composite aerogels, Julio C Arboleda, Orlando J Rojas,
Lucian A Lucia, Janne Laine
16. Surface functionalized nanofibrillar cellulose (NFC) film as a platform for immunoassays and diagnostics, Ilari
Filpponen, Hannes Orelma, Leena-Sisko Johansson, Monika Österberg, Orlando Rojas, Janne Laine
17.Novel Pretreatment in the Manufacture of Nanofibrillated Cellulose via Microfluidization , Carlos A Carrillo, Janne Laine,
Orlando J Rojas
18
23.5.2013
10
Orals:1. Nanoparticles and Nanostructures from Direct- and Self- Assembly of Components Cleaved from
Fiber Cell Walls, Orlando Rojas, North Carolina State & Aalto University2. 2-Dimensional Nanoscale Structures from Cellulosic Materials, Eero Kontturi, Aalto University3. Super-Strong Soy Protein/Nanocellulose Composite Aerogels, Julio Arboleda, North Carolina State
University4. Surface Assembly of Chemically Reactive Polysaccharides on Nanocellulose, Janne Laine, Aalto
University5. Magnetic Cellulose Nanocrystal Hybrid, Tiina Nypelö, North Carolina State University6. ZnO-Bacterial Cellulose Nanocrystal Composite and its Potential as Energy Harvesting Material,
Levente Csoka, University of West Hungary7. Surface Functionalized Nanofibrillar Cellulose (NFC) Film as a Platform for Immunoassays and
Diagnostics, Ilari Filpponen, Aalto University8. Nanofibrillated Cellulose as Carrier for Short Peptides Assemblies for Human IgG Detection and
Affinity Separation, Yanxia zhang, North Carolina State University9. Self-Assembly of Cellulose Fibrils/SiO2 Nanoparticles During Synthesis by Gluconacetobacter
Bacteria- Robin Zuluaga Gallego, Pontificia Bolivariana University
Posters:1. Reinforcing Nanocellulose Isolated from Banana Rachis and Corn Husk-Robin Zuluaga Gallego,
Pontificia Bolivariana University2. Hydrophobization of Cellulosic Substrates by Creating Surface Nanostructures Using Enzymatic
Methods-Oriol Cusola, Universitat Politècnica de Catalunya UPC-BarcelonaTech 19
Subjects1. Introduction and general report
(Orlando Rojas)2. Hydrolysis of bicomponent films
(Raquel Martin / OR)3. Hydrolysis of SEW nanofibers & BC
(Luis Morales)4. Novel methods in NFC production
(Carlos Carrillo / OR)5. SEW fibers, NFC and nanopaper
Cellulose Blend ratio 10:1 Blend ratio 5:1 Blend ratio 1:1
Enzymatic hydrolysis using QCM-D: enzymes mixture
Roughness increase:
Cellulose hydrolysis
Enzyme adsorption
Aft
er H
ydro
lysi
sB
efo
re H
ydro
lysi
s
EndoglucanaseExoglucanases (CBH-II)
Exoglucanases
(CBH-I)
Trichoderma cellulase enzymes
Enzyme Conc (%)
CBH I 50-60
CBH II 15-18
EG I 12-15
EG II 9-11
EG III 0-3
EG V 0-3
Monocomponents enzymes
Cellobiohydrolase or exoglucanase
Endoglucanase
23.5.2013
16
0 50 100 150 200 250 300 350 400-200
-160
-120
-80
-40
0
40
f 3(H
z)
Time (min)
Enzyme
injection
Buffer
rinsing
Ce/L 1:0
Ce/L 5:1
Ce/L 1:1
Ce/L 0:1
CBH-I adsorbs more on lignin than on cellulose.
Lignin reduce the hydrolysis rate (compare Ce/L 1:0 to 5:1) until complete inhibition for 1:1.
0 5 10 15 20 25 30 35 40-30
-20
-10
0
10
20
30
f 3(H
z)
Time (min)
Enzyme
injection
Stop
injection
Ce/L 1:0
Ce/L 5:1
Ce/L 1:1Ce/L 0:1
CBH I
40oC, pH 5, CBH I (0,25 mg/ml)
0 50 100 150 200 250 300
-140
-120
-100
-80
-60
-40
-20
0
20
40
60
Ce/L 1:1
f 3(H
z)
Time (min)
Buffer
rinsing
Enzyme
injection
Ce/L 0:1
Ce/L 1:0
Ce/L 5:1
EG-I are adsorbed on both lignin and cellulose
Lignin reduce the hydrolysis rate of cellulose (compare Ce/L 1:0 to 5:1 and 1:1) until almost complete inhibition.
More EG-I can be adsorbed on lignin after 20 minutes
0 5 10 15 20 25 30 35 40-30
-20
-10
0
10
20
30
40
50
f 3(H
z)
Time (min)
Stop
injection
Enzyme
injection
Ce/L 1:0
Ce/L 5:1
Ce/L 1:1
Ce/L 0:1
EG I
40oC, pH 5, EG I (0,25 mg/ml)
23.5.2013
17
After long adsorption stage Inhibition of hydrolysis in cellulose films Overcrowding
The dense binding of enzyme molecules hinder each other from accessing the cellulose with their catalytic domains (Suchy et al. Langmuir 2011, 27, 8879-8828)
Effect of lignin content on the properties of nanopapers: Mechanical properties
4 %
14 %
0 %
100
200
300
4
8
12
16
A B C D E F G H I J K L0
3
6
9
12
Str
ength
(M
Pa)
Modulu
s (
GP
a)
Str
ain
(%
)
Softwood Hardw. Non-w.
A
A : Present work
B-L : Literature values- L
+ L
23.5.2013
37
Synthesis and characterization of soy protein-nanocellulose
composite aerogels
Subjects1. Introduction and general report
(Orlando Rojas)2. Hydrolysis of bicomponent films
(Raquel Martin / OR)3. Hydrolysis of SEW nanofibers & BC
(Luis Morales)4. Novel methods in NFC production
(Carlos Carrillo / OR)5. SEW fibers, NFC and nanopaper
(Ester Rojo)6. NFC aerogels with SPs
(Julio Arboleda/OR)7. NFLC aerogels
(Mariko Ago/OR)8. Laccase-mediated coupling
(Oriol Cusola / OR)9. Carbon nanodots
(Kaoliina Junka / OR)10. CNC modeling
(Henry Bock)11. Surface chemistries
(Ilari Filpponen)12. Asymmetric CNC modification
(Arcot Lokanathan)
USA crop value (2011) $35.7 billionUSA exports (2011) $21.5 billionMain use Soy oil Protein for human food 5%
American Soybean Association, Soy Stats 2011 World Agricultural Supply and Demand Estimates, 2012United Soybean Board, 2012
Relevance of soy bean
Soybean production is a world-scale business and generates large amounts of residual proteins
74
23.5.2013
38
Pro-Fam 955. Isolated Soy Protein. Data Sheet. ADM. 2009
Soy proteins-chemical diversity
Aromatic10.1%
Hydrophobic34.3%
Nucleophilic10.4%
Hydroxyl9.2% Other (small)
8.4%
Acids/Esters30.7%
Cationic16.5%
75
It is a porous material resulting from removing the solvent from a regular gel
What is an aerogel?
Aerogels are extensively used for aerospace applications, such as insulation for launch vehicles or for planetary entry, descent, and landing systems
In 1997 silica aerogels were used for the first time in the Mars Pathfinder mission
Meador, et Al. Applied Materials & Interfaces. 2012. 4, 536-544
Mech. strength between 5 – 9MPa
76
23.5.2013
39
Because of their high specific surface area and low density aerogels can be used in different applications
Aerogels
Thermal isolation
Non wovens
Filters
Packaging
Absorption Surface chemistry and catalysis
Packaging
77
Not every gel can be dried to form an aerogel
Aerogels
Even freeze drying induces stress and collapse
Only ”strong” materials can form aerogels by freeze drying
Gelatin collapses by freeze drying
Capillary forces in conventional drying
78
23.5.2013
40
Soy proteins alone tend to form brittle structures. Reinforcement with cellulose nano fibers (CNF) was studied
Cellulose Nano Fibers (CNF) reinforcement
CNFs can form aerogels by themselves, in this work the synergistic effect of both materials is studied
79
Under certain conditions (T and pH) Soy Protein (SP) denatures forming a viscous gel. The gel can be frozen and dried to form an aerogel
Aerogels from soy proteins
T: 70 CHCl 0.09 N1 h10% SP
SP – NFC Mixtures8% Solids
Slow Freezing
80
23.5.2013
41
Now Before
If not freezing is used the material flow out of the containers.
Procedure used successfully for CNF alone
If the freezing is too fast the material experience a fast expansion and it may break
Channels may be formed following temperature gradients
Munch, 2008
In presence of soy proteins conventional techniques are not adequate
Vacuum drying Fast freezing Directional freezing
81
82
23.5.2013
42
Surface area of CNF aerogels is 10 times lower compared with fast freezing processes
Surface area decreases when SP is added
SP Increment
Surface Area (BET)
83
The samples were weighted after drying and then left in a room in controlled conditions (23 °C, 50% relative humidity). The water uptake was followed
Equilibrium moisture is very similar in aerogels with different protein content
Water uptake from air
84
23.5.2013
43
Aerogels do not collapse under stress of 5 MPa
Stress required for 18% deformation
Mechanical properties
are similar for aerogels with reinforcing CNF of 33% or higher
Compression tests
CNF works as reinforcing agent
85
Water sorption is modulated by the composition
Hexane sorption is fast in all cases
Wat
er s
orp
tio
nH
exan
e so
rpti
on
Wicking tests
86
23.5.2013
44
Gels with higher SP content absorb more water
Excellent aerogel integrity (water and hexane)
SP Increment
Absorption by immersion
Water
Hexane
87
• Soy protein aerogels were produced by freeze drying
• Slow drying: avoids channel formation
• CNF reinforcing effect
• Soy protein decreases the rate of water uptake by the aerogels.
• Tunable water loading capacity
Conclusions
88
23.5.2013
45
Subjects1. Introduction and general report
(Orlando Rojas)2. Hydrolysis of bicomponent films
(Raquel Martin / OR)3. Hydrolysis of SEW nanofibers & BC
(Luis Morales)4. Novel methods in NFC production
(Carlos Carrillo / OR)5. SEW fibers, NFC and nanopaper
(Ester Rojo)6. NFC aerogels with SPs
(Julio Arboleda/OR)7. NFLC aerogels/foams
(Mariko Ago/OR)8. Laccase-mediated coupling
(Oriol Cusola / OR)9. Carbon nanodots
(Kaoliina Junka / OR)10. CNC modeling
(Henry Bock)11. Surface chemistries
(Ilari Filpponen)12. Asymmetric CNC modification
(Arcot Lokanathan)
Development of Bio-based
porous material and lignin
functionalization
NFC for porous material; high strength, high aspect ratio will allow to form rigid networks though interfibrils bondingsincluding physical entanglement and hydrogen bonds.
Amylopectin;Highly branched polysaccharide, semicrystal, Low cost, biodegradability, and renewal
In this study, NFC from EFB origin (provided from Dr. Ana Ferrer) with various content of lignin was used for the composited with amylopectin to investigate
-effect of lignin content on morphology and physical properties.-effect of compositions of NFC and amylopetin
Porous materials forInsulation, cushioning protection, catalysis, membrane, biomedical, construction materials
Water reservation
Tissue engineering
Cushioning protection
catalysis
NFLC/amylopectin biocomposite
Development of Bio-based porous material
23.5.2013
46
NFLC: Three different EFB pulp grades were produced after sulfur-free chemical treatments:
Composition of NFC-N or NFC-F and Amylopectin (w/w)
Table 1. EFB pulp chemical composition in weight percent (data rounded off to the first significant figure). Short notation N, M and F are used for NaOH-AQ, Milox and FoOH, respectively
Pulp type α-Cellulose Hemicellulose Holocellulose LigninEthanol
extractivesAsh
NaOH-AQ: N 81.8 15.9 97.7 2.3 2.5 1.0
Milox: M 63.4 22.1 85.5 6.2 5.1 1.6
FoOH: F 75.8 6.2 82 9 8.6 1.7
NFC-N ; at -20 oC
NFC-F; at -10 oC
Amylopectin soluble in liquid (water)
NFC
Freeze dry
Freezing temperature
wet gel aerogel
Air (void)
SolidSkeleton
NFCamylopect
insolid content
wt%
NFC-N(-200C)
100 0 1.675 25 2.0
50 50 3.1
25 75 6
0 100 5
NFC-F(-10 OC)
100 0 5
75 25 5
50 50 5
25 75 5
0 100 5
-Regeneration (crystallization) of amylopectin-Ice nucleatingThese factors can be influenced on the structure.
Pulp typeα-
CelluloseHemicellulose Holocellulose Lignin
Ethanol
extractivesAsh
1 NaOH-AQ: N 81.8 15.9 97.7 2.3 2.5 1.0
2 Milox: M 63.4 22.1 85.5 6.2 5.1 1.6
3 FoOH: F 75.8 6.2 82 9 8.6 1.7
4NFC (bleaced, from birch), prepared by Anu
--* -- -- -- -- --
NFLCs for biofoam with amylopectin
Preparation of NFC; Mechanical treatment1.5 wt% suspensions in D. I. waterMicrofluidaizer; 5passes
Chemical compositions of NFCs used for composite biofoam
Amylopectin (AP)from waxy corn
AP solution (water)NFC
Freeze dry
wet gel biofoam
Air (void)
SolidSkeleton
* -- Not measured
23.5.2013
47
NFC AP solid contentwt%
Densitymg/cm3
NFC-N(-200C)
100 0 1.6 26
75 25 2.0 45
50 50 3.1 50
25 75 6 118
0 100 5 98
NFC-M(-10 OC)-01, -02
100 0 3
75 25 3
50 50 3
25 75 3
0 100 3
NFC-F(-10 OC)
-01 (solid,5wt%),
-02 (3wt%)
100 0 5 or 3 82
75 25 5 or 3 67
50 50 5 or 3 57
25 75 5 or 3 49
0 100 5 or 3 53
NFC-01, -02(-10 OC)
100 0 3 360
75 25 3 61
50 50 3 56
25 75 3 34
0 100 3 36
NFC/AP (w/w)
100/0 75/25 50/50 25/75 0/100
AP
Weight ratio and densiy of biofoams with various types of NFC and AP
NFC
Preparation of biofoam NFLC and AP
Water adsorption test
NFLC-N /Amylopectin and NFLC-F/Amylopectin composites after freeze-drying
NFC/Amylopectin (w/w)
100/0 75/25 50/50 25/75 0/100
NFLC
NFLC-N
NFLC-F
amylopectin
-20 oC
-10 oC
•Density of the composite was increased with increasing of NFC content.
Density of the composites with various NFLC-F amount •Two different NFLC and amylopectin composites were
prepared by freeze-drying with various compositions.
0 20 40 60 80 1000.00
0.02
0.04
0.06
0.08
0.10
NFC-F, wt%
den
sity
, g/c
m3
0
2
4
6
8
10
solid
, wt%
•Lower temperature (-20 oC) for freeze-dry was found less defects on the morphology, especially NFLC dominant composition.
•How dose freezing temperature affect on the structure? Amylopectin crystallizing + ice nucleating
23.5.2013
48
NFC/AP
NFC-F/AP 99 mg/g
67 mg/g 64 mg/g55 mg/g53 mg/g
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0 50 100 150 200 250
0.00
0.02
0.04
0.06
0.08
0.10
wat
er r
egai
n, g
/g
25
NFC, 0
50
75
100
25
wat
er r
egai
n, g
/g
time, min
NFC, 0
50
75
100
30 mg/g
27 mg/g
22 mg/g
20 mg/g
11 mg/g
Water regain (4h)Condition: NaCl aq. Sat (22 oC)
Water adsoption test on biofoams
Water adsorption of NFLCs/AP bio foam was increased with increasing AP ratio in the biofoam.
NFLC-F containing 9 % lignin and AP biofoams showed higher water adsorption than NFLC (bleached)/AP biofoams.
Both NFC0 (AP100) in foams of NFLC and NFLC-F were supposed to be similar, but they were different in the value of water regain. Need to check the procedure of preparation of biofoam.
23.5.2013
49
New lignin based nancomposite material with Click reaction
Lignin beadsCrosslink withEpichlorohydr
in
Surface modification(alkynylation) of lignin beads