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BASIC PRINCIPLES | Steam Sterilization in laboratories
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Sterilization of solids(Preparation)
Sterilization of liquids(Preparation)
Sterilization of waste(Decontamination)
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STERILIZATION OF LIQUIDS (PREPARATION)
• Culture media in bottles to selectively grow specific microorganisms
• Instruments• Empty glassware• Tubes and hoses• Pipettes, pipette tips• Filters, Textiles
BASIC PRINCIPLES | steam sterilization in laboratories
STERILIZATION OF SOLIDS (PREPARATION)
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• Molecules or particles that have even been released from the surface of a liquid create a pressure against the environment (steam pressure)
• The steam pressure of a liquid is solely dependent on its temperature (kinetic energy)
• The liquid starts to evaporate, when the steam pressure is as high as the ambient pressure
The ambient (atmospheric) pressure (at sea level) is 100 kPa
Water is evaporating when the pressure in the liquid is equal to the ambient pressure
BASIC PRINCIPLES | steam pressure
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• When steam condenses e.g. on cold surfaces, steam releases its kinetic energy (heat)
• Due to condensation, the aggregate state changes from gaseous (steam) to liquid
BASIC PRINCIPLES | condensation
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STEAM GENERATION BY INTERNAL HEATING ELEMENTS
• Heating elements inside the pressure vessel of the autoclave
• Steam is generated in a steam generator which is separated from the pressure vessel but integrated into the housing of the autoclave
BASIC PRINCIPLES | how is steam produced in steam sterilizers
STEAM GENERATION BY SEPARATE STEAM GENERATOR
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H: Heat-upS:
Sterilization C: Cooling
Blue: Pressure in
the chamberRed:
Temperature in the
media
• Cooling by evaporation down to 80°C
Cooling time between 1 hour and 5 hours (depending on load volume)
STERILIZATION OF LIQUIDS | cooling phase | ambient air ventilation
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• Air displacement by simple heat-up• Steam atmosphere in the pressure
vessel• Air inside the waste bag
No sterilizing effect in the waste bag
STERILIZATION OF WASTE | heat-up phase | inefficient air release
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Air Steam
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STEAM GENERATION BY INTERNAL HEATING ELEMENTS
• Heating elements inside the pressure vessel of the autoclave
• Demineralized water is evaporated directly inside the chamber
• Steam is generated in a steam generator which is separated from the pressure vessel but integrated into the housing of the autoclave
BASIC PRINCIPLES | how is steam produced in steam sterilizers
STEAM GENERATION BY SEPARATE STEAM GENERATOR
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• Pipette tip box wrapped with aluminum foil
STERILIZATION OF SOLIDS | heat-up phase | inefficient air release
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STERILIZATION OF LIQUIDS | cooling phase | ambient air ventilation
OPEN VESSEL (1000ml water)CLOSED VESSEL (1000ml water)
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FOR MORE INFORMATION CONTACT US
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