Sta$s$cs for Genomics (140.688) Instructor: Jeff Leek Slide Credits: Paul Robinson (Purdue), ABDSerotec, Raphael Gottardo (FHCRC), http://www.usuhs.mil/bic/cytometry/ pdf/tutorial.pdf. 5/14/10 – Rough draft of project due. Please send with subject line: [project rough draft]
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Stascs for Genomics (140.688) - Biostatistics - Departments - …jleek/teaching/genomics/flow... · · 2010-05-05Publicaons using the keyword “ flow cytometry” from 7:36 AM
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Sta$s$csforGenomics(140.688)
Instructor: Jeff Leek
Slide Credits: Paul Robinson (Purdue), ABDSerotec, Raphael Gottardo (FHCRC), http://www.usuhs.mil/bic/cytometry/
pdf/tutorial.pdf.
5/14/10 – Rough draft of project due. Please send with subject line: [project rough draft]
BasicProblem
Abunchofcells
(1) Whattypeofcellsarethere?
(2) Whatfrac$onareapar$culartype?
(3)Whatproper$esdothecellshave?
(4)Canwesortthecellsbytype?
ThingsYouCanMeasureWithAFlowCytometer
1. Size and complexity of cells 2. Cell pigments 3. Copy number variation (Flow-FISH) 4. Protein expression/localization 5. Nuclear antigens 6. Cell surface molecules