Standards for public health genomic epidemiology to improve infec7ous disease outbreak detec7on and inves7ga7on Simon Fraser University and BC Public Health Microbiology and Research Laboratory 8 th Interna*onal Biocura*on Conference, April 26th 2015 1 Mélanie Courtot, Ph.D. [email protected]@mcourtot h$p://www.slideshare.net/mcourtot
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Standards for public health genomic epidemiology - Biocuration 2015
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Standards for public health genomic epidemiology to improve
infec7ous disease outbreak detec7on and inves7ga7on
Simon Fraser University and BC Public Health Microbiology and Research Laboratory
8th Interna*onal Biocura*on Conference, April 26th 2015
2 Source: Interna-onal Associa-on for Food Protec-on
3
Each year, one in eight Canadians (or four million people)
get sick with a domes7cally acquired food-‐borne illness.
4 Source: Peter Gleick, Scienceblogs.com
5
6
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Current Methods of Characterizing Foodborne Pathogens in a Public
Health Laboratory
8
• Growth characterisKcs • Phenotypic panels • AggluKnaKon reacKons • Enzyme immuno assays (EIAs) • PCR • DNA arrays (hybridizaKon) • Sanger sequencing • DNA restricKon • Electrophoresis (PFGE, capillary) Each pathogen is characterized by methods that are specific to that pathogen in mulKple workflows (separate workflows for each pathogen) TAT: 5 min – weeks (months)
Source: Rebecca Lindsey
9
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Epidemiology Case centric
Clinical Patient centric
Laboratory Test centric
11 Source: Jennifer Gardy
12
2011 E. coli O104:H4 German outbreak
-‐ 53 deaths, 3950 ill -‐ Farmers compensated €210 million by EU
Germany
Egypt
Source: Fiona Brinkman
13
NationalPublic Health Agency
Provincial Public Health Agency Academic/Public
13
The IRIDA pla^orm (Integrated Rapid InfecKous
Disease Analysis) fills in selected key
bioinformaKcs gaps, to support real-‐Kme infecKous
disease outbreak invesKgaKons in Canada
Open source, standards compliant, resource for public health agencies that can interact with, and complement, other ini-a-ves
14 14
IRIDA Annual General Mee7ng Winnipeg, April 8-‐9, 2015
• Load exis7ng datasets on the first version of IRIDA
• Annotate and integrate the data
• Validate the ontology content and its implementa7on
26 Source: Damion Dooley
INTERFACE SUPPORT Enable flexible user-‐specific interface
27 h$p://pathogenomics.sfu.ca/islandviewer
IslandViewer Dhillon and Laird et al. 2015, Nucleic Acids Research
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IRIDA SNVPhyl pipeline output in GenGis, unpublished
h$p://kiwi.cs.dal.ca/GenGIS/Main_Page
IRIDA SNVPhyl pipeline
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#1. Genomic epidemiology can help detect infecKous disease outbreak earlier #2. Integrated Rapid Infec7ous Disease Analysis plaaorm deployed in Canada, supported by a set of ontologies and standards #3. IRIDA capitalizes on exis7ng resources to make genomic epidemiology a pracKcal reality
Acknowledgements Project Leaders
Fiona Brinkman – SFU Will Hsiao – PHMRL Gary Van Domselaar – NML University of Lisbon Joᾶo Carriҫo Na7onal Microbiology Laboratory (NML) Franklin Bristow Aaron Petkau Thomas Ma$hews Josh Adam Adam Olsen Tara Lynch Shaun Tyler Philip Mabon Philip Au Celine Nadon Ma$hew Stuart-‐Edwards Morag Graham Chrystal Berry Lorelee Tsche$er Laboratory for Foodborne Zoonoses (LFZ) Eduardo Toboada Peter Kruczkiewicz Chad Laing Vic Gannon Ma$hew Whiteside Ross Duncan Steven Mutschall
Simon Fraser University (SFU) Melanie Courtot Emma Griffiths Geoff Winsor Julie Shay Ma$hew Laird Bhav Dhillon Raymond Lo BC Public Health Microbiology & Reference Laboratory (PHMRL) and BC Centre for Disease Control (BCCDC) Judy Isaac-‐Renton Patrick Tang Natalie Prystajecky Jennifer Gardy Damion Dooley Linda Hoang Kim MacDonald Yin Chang Eleni Galanis Marsha Taylor Cletus D’Souza Ana Paccagnella University of Maryland Lynn Schriml Canadian Food Inspec7on Agency (CFIA) Burton Blais Catherine Carrillo Dominic Lambert Dalhousie University Rob Beiko Alex Keddy
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McMaster University Andrew McArthur Daim Sardar European Nucleo7de Archive Guy Cochrane Petra ten Hoopen Clara Amid European Food Safety Agency Leibana Criado Ernesto Vernazza Francesco Rizzi ValenKna