Srinivas Garlapati Vaccine and Infectious Disease Organization Saskatoon, Canada zing vaccine formulation and delivery to improve human and animal h Partnership development activity (PDA) between Canada and India funded by ISTP, Canada
Srinivas Garlapati
Vaccine and Infectious Disease OrganizationSaskatoon, Canada
Optimizing vaccine formulation and delivery to improve human and animal healthPartnership development activity (PDA) between Canada and India
funded by ISTP, Canada
Courtesy: Parallel solutions Inc Andrianov et al, 1998
1000X 5000X
OVA CpG Merge
Garlapati et al 2008
Garlapati et al 2010
Alum SOL
MP
MP
Single step process by simple mixing PCEP with natural poly-amine Chitosan or synthetic poly-ally-amine hydrochloride (PAH) – FDA approved
NaCl-CaCl2 PAH
Chitosan
PCEP- CaCl2 MPs
PCEP- PAH MPs
PCEP- CHIT MPs
PCEP particle uptake by DCsPCEP particle uptake by DCs
Garlapati et al manuscript in submission
Garlapati et al manuscript in submission
RSV MARKET POTENTIAL
Discovery Pre-Clinical Phase-I Phase-II Phase-III2020
MEDI-559MEDI-534 (PIV3)
Novavax VLP F & G
PEV4 (virosome)
Protollin- RSV
RSV287Nanobio RSV
Sendai Virus vectorAlpha Virus vector
Virosomes P3CSK4rRSVΔG
DNA electoporation
Adenovirus vector
0 21 44 48 50
Immunize Boost Challenge
Kill 1
Kill 2
Spleens - ELISPOTLung-Fragment culturesBAL
Groups
1.Saline/RSV challenge2.Ag IN3.Ag Triple Combo SOL IN4.Ag Triple Combo MP IN5.Ag IM6.Ag Triple Combo SOL IM7.Ag Triple Combo MP IM
Triple Combo formulation with ΔF protein
Viral SheddingLung Cytokines
RSV-specific humoral responses in serum.
Serum Virus Neutralization TitersVirus Titers
Lung Fragment Culture
Cytokine ELISPOT of Splenocytes
Cytokines in Lung Homogenates Post-RSV Challenge
Acknowledgements….Acknowledgements….
Nelson EngGael AurayTadele KirosRachelle BuchananStacy StromMonika PolewiczAleksandra GraciaLaura LatimerMarlene SniderRavinder Garg
Volker GerdtsLorne BabiukAndrew PotterSylvia v.d. HurkHeather Wilson George MutwiriJim HolmstromHugh Townsend
VIDO
Funding: - B. & M. Gates Foundation - Canadian Institute of Health Research - Krembil Foundation - National Science and Engineering Research Council - Saskatchewan Health Research Foundation
Animal CareBarry WalkerSherryOthers…