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Chiral Integrated Catalysts Composed of Bifunctional Thiourea and Arylboronic Acid: Asymmetric Aza-Michael
Addition of α,β-Unsaturated Carboxylic Acids
Noboru Hayama, Takumi Azuma, Yusuke Kobayashi, and Yoshiji Takemoto*Graduate School of Pharmaceutical Sciences, Kyoto University;
Yoshida, Sakyo-ku, Kyoto 606–8501, Japan.Received December 2, 2015; accepted December 21, 2015
The first intermolecular asymmetric Michael addition of nitrogen-nucleophiles to α,β-unsaturated car-boxylic acids was achieved through a new type of arylboronic acid equipped with chiral aminothiourea. The use of BnONH2 as a nucleophile gives a range of enantioenriched β-(benzyloxy)amino acid derivatives in good yields and with high enantioselectivity (up to 90% yield, 97% enantiomeric excess (ee)). The obtained products are efficiently converted to optically active β-amino acid and 1,2-diamine derivatives.
Stereoselective functionalization of electron-deficient al-kenes continues to play a fundamental role in modern organic synthesis. The catalytic asymmetric aza-Michael addition of α,β-unsaturated carboxylic acid derivatives has been exten-sively investigated as a powerful protocol for the synthesis of optically active β-amino acids,1–4) which are frequently encountered as important structural components in biologi-cally active natural products and pharmaceuticals.5) However, simple α,β-unsaturated esters and amides have only been used in a limited number of successful studies because of their inherent low reactivity as Michael acceptors.6,7) Instead, a variety of activated ester/amide surrogates possessing greater reactivity and rigidity, which enables them to interact with appropriate catalysts, have been developed for the Michael addition.8) Recently, a range of activated carboxylates—spe-cifically, α,β-unsaturated imides,9,10) acyl pyrroles,11) and acyl pyrazoles12)—have been successfully applied in asymmetric metal- and organocatalyzed Michael additions to furnish the desired products with excellent enantioselectivity (Chart 1). However, this masked ester/amide strategy requires a stoichio-metric amount of an activating auxiliary and additional pro-tection and deprotection steps. From the perspective of atom and step economy, achieving a direct Michael reaction of α,β-unsaturated carboxylic acids without the use of any activating templates is highly desirable. Despite strong synthetic utility, the direct Michael addition of α,β-unsaturated carboxylic acids has never been reported, except for an enzyme-catalyzed aza-Michael addition13,14); very few strategies have been exploited to catalytically activate the hydroxy group of carboxylic acids via transient covalent bond formation.
By contrast, since the discovery by Yamamoto and Ishihara that electron-deficient arylboronic acids are efficient activators of carboxylic acids in direct amidation reactions,15–18) a series of arylboronic acids have been developed for the activation of
various functional groups, including α,β-unsaturated carbox-ylic acids,19–21) alcohols,22) carbonyl compounds,23) and oth-ers.24,25) Hall reported that different types of arylboronic acids could be used for the cycloaddition of α,β-unsaturated carbox-ylic acids to dienes and 1,3-dipoles.20,21) Recently, we also re-vealed that aminoarylboronic acid was the best catalyst for the intramolecular hetero-Michael addition of α,β-unsaturated car-boxylic acids bearing sulfonamides or phenols.26) In addition, the asymmetric oxa-Michael addition could be achieved via a dual catalysis system consisting of bifunctional thiourea27) and 3,5-bis-(trifluoromethyl) phenylboronic acid. On the basis of these results, we explored two activation strategies for the intermolecular asymmetric aza-Michael addition of α,β-unsaturated carboxylic acids (Chart 2): (i) synergistic activa-tion of a nucleophile and an electrophile using a bimolecular dual catalyst system, and (ii) dual activation of a carboxylic acid moiety using structurally integrated catalysts composed of arylboronic acid and bifunctional N-arylthiourea moi-eties.28,29) To perform the target reaction, both regioselectivity and enantioselectivity must be controlled by these catalytic systems. Herein, we report that a novel chiral hybrid catalyst remarkably promoted the intermolecular aza-Michael addition of O-benzylhydroxylamine to a wide range of α,β-unsaturated carboxylic acids, providing the Michael adducts with complete regioselectivity and high enantioselectivity.
Results and DiscussionWe initially examined the aza-Michael addition of (E)-
5-phenylpent-2-enoic acid 1a with BnONH2 under dual cata-lytic conditions [strategy (i)] using achiral arylboronic acids4a–c and aminothiourea 5 (Table 1). The reaction was per-formed in CCl4 at room temperature with 10 mol% of each cat-alyst and 4 Å molecular sieves. Although the electron-deficient arylboronic acid 4a gave no desired product 2a, affording
* To whom correspondence should be addressed. e-mail: [email protected]
Special Collection of Papers
This article is dedicated to Professor Satoshi Ōmura in celebration of his 2015 Nobel Prize.
Vol. 64, No. 7 (2016) 705Chem. Pharm. Bull.
only double-addition adduct 3a in moderate yield, mixtures of 2a and 3a were obtained with other catalyst combinations 4b/5 and c/5, giving only racemic 2a in low yields. To iden-tify the catalytic effect of co-catalyst 5, we then attempted the same reaction of 1a in the absence of 5, which clarified that merely using catalysts 4a–c gave almost the same results as the dual catalytic systems. These results indicate that, in contrast to the intramolecular Michael addition of phenols and sulfonamides,26) arylboronic acids 4 singularly promote the
Michael addition of BnONH2 and that chiral thiourea 5 does not appear to participate in the activation of substrates in this catalytic cycle.
Because the dual catalysis system composed of arylboronic acid 4 and bifunctional thiourea 5 proved to be ineffective for the intermolecular Michael addition, we next designed a new type of catalyst 6 in which arylboronic acid 4b and ami-nothiourea 5 were merged. We envisioned that the integrated catalyst would efficiently activate carboxylate anions via both Lewis acid-Lewis base and hydrogen-bonding interactions and that 1,2-addition of the nucleophile might be inhibited because of the crowded environment around the carboxylate complex (see strategy (ii) in Chart 2). To evaluate the functionality of the proposed catalysts, a series of integrated arylboronic acids 6a–i bearing different hydrogen-bond-donating moieties were prepared and subsequently screened under various reaction conditions; the results are summarized in Table 2. As expect-ed, all integrated catalysts, except sulfonamide 6c, afforded the desired β-benzyloxyamino acid 2a as a single product; in addition, the nature of the hydrogen-bond-donor moiety was observed to substantially affect the yield. Among catalysts 6a–e, thiourea 6e bearing a 3,5-bis(trifluoromethyl) phenyl group gave the best results in terms of yield and enantio-selectivity (entries 1–5). Regarding the achiral N-substituent of the thiourea moiety, catalysts 6g and i bearing phenyl and 4-nitrophenyl groups, respectively, exhibited higher enanti-
Chart 1. Direct Michael Addition to α,β-Unsaturated Carboxylic Acids
Chart 2. Asymmetric Michael Addition via (i) Dual Catalysis and (ii) Integrated Catalysis
Table 1. Intermolecular Aza-Michael Addition Catalyzed by 4a–c and Co-catalyst 5
Catalyst 2a 3a
(a) 4a and 5: 0% 41%(b) 4b and 5: 15% (0% ee) 6%(c) 4c and 5: 29% (0% ee) 22%
Table 2. Optimization of the Aza-Michael Addition of R1ONH2 to 1aa)
a) Unless otherwise noted, the reaction was carried out with 1a (1.0 mmol), nu-cleophile (1.0 equiv), and catalyst 6a–i (0.1 equiv) at room temperature for 24 h. b) Isolated yield after treatment with TMSCHN2. c) Estimated by chiral HPLC after treatment with TMSCHN2. d) Without 4 Å molecular sieves.
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oselectivities than electron-rich derivatives 6f and h (entries 6–9). Further investigation of R1ONH2 and solvent revealed that the highest ee was obtained when the reaction was per-formed with 6i in CCl4 at room temperature in the presence of 4 Å molecular sieves, furnishing 2a in 83% yield with 90% enatiomeric excess (ee) (entries 9–14).30) Notably, the reaction gave no desired product in the absence of 4 Å molecular sieves in any solvent (entry 15).
Under the optimized conditions, we next examined the sub-strate scope of the aza-Michael addition catalyzed by 6i (Chart 3). Aliphatic substrates possessing different alkyl chains 1b–f were initially screened as β-alkyl amino acids, which have proven to be more challenging synthetic targets. Irrespective of alkyl chain length, the reaction of the linear alkyl sub-strates 1b–f proceeded smoothly to give the corresponding products in good yields and with high ee’s (2b–f: 86-97% ee’s). In contrast, a lower ee was observed with the branched aliphatic substrate 1g (2g: 76% ee). Other aliphatic substrates bearing ether, ester and sulfide moieties 1h–j were well-toler-ated (2h–j: 85-91% ee’s); however, the Cbz-carbamate 1k gave only a moderate ee. A variety of (E)-5-arylpent-2-enoic acids 1l–q bearing electron-deficient and electron-rich aryl and het-eroaryl groups were used as Michael acceptors to afford the corresponding β-amino acid derivatives in good yields and with high enantioselectivities (2l–q: 83–93% ee’s). We also evaluated a series of other aryl-substituted 2-alkenoic acids; substrates 1r and s, with a phenyl group at their 6- and 7-posi-tions, respectively, both underwent the Michael addition to de-
liver products with no loss of yield or enantioselectivity (2r, s: 88–91% ee’s); however, introduction of a phenyl group at the 4-position resulted in a substantial decrease in enantioselec-tivity (2t: 71% ee) with no influence on the chemical yield. To our surprise, the same reaction with cinnamic acid (1u: R=Ph) did not proceed, resulting only in recovered starting material. The absolute configuration of products 2b–t are presumed to have the S configuration.30)
To gain insight into the reaction mechanism, the Michael addition with (Z)-4-benzyloxybut-2-enoic acid (Z)-1h was performed under the optimized conditions, providing the op-posite enantiomer, ent-2h, in 76% yield and with a slightly lower enantioselectivity (83% ee) (Chart 4). We next explored the use of other nitrogen nucleophiles to extend the syn-thetic utility of the reaction and observed that Bn2NNH2 and CbzNHNH2 underwent the Michael addition under the same conditions, giving the corresponding adducts 7 and 8 with en-antioselectivities similar to those of (benzyloxy) amine deriva-tives 2a–s. However, 20 mol% of 6i was used for the reaction of 1b with CbzNHNH2, since a lower yield of 8 was observed (48%). Because the resulting hydrazine 8 possesses the same absolute configuration as 2b,30) the aza-Michael addition is considered to occur via a similar catalytic process, indepen-dent of the nucleophiles employed.
In the integrated catalysts, a Brønsted basic site together with Lewis acidic and hydrogen-bonding sites (i.e., a tri-coordinate boron atom and two thiourea–NH protons) are available to activate both acidic and basic substrates, such as a carboxylic acid. On the basis of the working hypothesis, together with the aforementioned experimental results, we propose the following plausible reaction mechanism31) (Fig. 1): Deprotonation of carboxylic acid 1 by catalyst 6 results in the formation of binary complex A or B via coordination of the resulting carboxylate anion to a boron atom; multiple hydrogen-bonding interactions between the carbonyl moiety and both the N–H protons of thiourea and the hydroxy group of the borate anion would also be present. Participation of the borate hydroxy group facilitates preferential attack of the nu-cleophile on the s-trans form of the carboxylate anion (binary complex B), which, after protonation of the enolate intermedi-ate C, provides (S)-adduct 2 in a highly enantioselective man-ner. The proposed mechanism rationalizes the need for the electron-deficient thiourea as a hydrogen-bond donor as well as the favored (S)-configuration of the products.
Finally, as a synthetic application of the aza-Michael addi-
Chart 3. Scope of the Substrates 1 under the Optimized Conditions
Chart 4. Scope of the Nucleophiles with (Z)- and (E)-Substrates
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tion, the asymmetric synthesis of (R)-3-aminododecanoic acid 10,30) which is a structural motif of the cyclic peptide epichli-cin, was performed starting from undecanal and malonic acid in a three-step sequence via tridec-2-enoic acid 9 (60, 90% ee) (Chart 5). Further synthetic utility was demonstrated by the one-pot transformation of α,β-unsaturated carboxylic acid 1a into enantio-enriched 1,2-diamine derivative 11 (68% yield, 89% ee) without isolation of the Michael adduct 2a.
SummaryWe have developed an intermolecular asymmetric aza-
Michael addition of α,β-unsaturated carboxylic acids using new chiral integrated catalysts composed of arylboronic acid and bifunctional thiourea moieties. A wide range of β-amino acid derivatives could be rapidly and concisely synthesized with good to high enantioselectivity (up to 97% ee). The util-ity of these products was illustrated by the conversion into both an important β-amino acid fragment of biologically ac-tive peptides and a vic-diamine derivative. We believe that our concept will stimulate further development of innovative asymmetric reaction protocols through catalytic activation of hydroxy groups of various functional groups. Efforts to dis-cover additional synthetic applications of our chiral integrated catalysts and to clarify the detailed reaction mechanism are currently underway in our laboratory.
ExperimentalGeneral Experimental Details All non-aqueous reactions
were carried out under a positive atmosphere of argon in dried glassware unless otherwise noted. Solvents and materials were obtained from commercial suppliers and used without further purification. Column chromatography was performed on Cica silica gel 60 (230–400 mesh) or Fuji Silysia silica gel (NH, 100–200 mesh). Reactions and chromatography frac-tions were analyzed employing pre-coated silica gel plate (Merck Silica Gel 60 F254). All melting points were measured on BÜCHI M-565 melting point apparatus and are uncor-rected. IR spectra were measured on JASCO FT/IR-4100. Unless otherwise noted, NMR spectra were obtained in CDCl3. 1H-NMR (400 MHz) spectra were recorded with JEOL ECP-400 spectrometers. Chemical shifts are reported relative to Me4Si (δ 0.0) in CDCl3, and residual solvents of CD3CN (δ 1.94), acetone-d6 (δ 2.05) and CD3OD (δ 3.31). 13C-NMR (100 MHz) spectra were also recorded using JEOL ECP-400 spectrometers. Chemical shifts are reported relative to CDCl3 (δ 77.0), CD3CN (δ 1.32, 118.3), acetone-d6 (δ 29.8, 206.3) and CD3OD (δ 49.0). 1H-NMR multiplicities are reported as fol-lows: br=broad; m=multiplet; s=singlet; d=doublet; t=triplet; q=quartet. High-resolution (HR)-MS were obtained on a Shimadzu LCMS-IT-TOF fitted with an electrospray ionization (ESI). Optical rotations were recorded on a JASCO P-2200 polarimater with a path length of 1 cm; concentrations are quoted in grams per 100 mL. [α]D values are measured in 10−1 deg cm2 g−1. Enantiomeric excess was determined by HPLC analyses. Unless otherwise noted, all materials and solvent were purchased from Tokyo Kasei Co., Aldrich Inc., and other commercial suppliers and were used without purification. All non-commercially available substrates were prepared accord-ing to the literature procedure as indicated below.
Preparation of α,β-Unsaturated Carboxylic Acidsα,β-Unsaturated carboxylic acids 1b–f, and 1u were com-
mercially available. 1k was prepared according to the litera-ture procedure.26) Other α,β-unsaturated carboxylic acids 1a, g, and l–q were prepared by following the general procedure as indicated below.
General Procedure for the Preparation of α,β-Unsaturated Carboxylic Acids (Procedure A)
3-Phenylpropionaldehyde (2.68 g, 20 mmol) and malonic acid (2.08 g, 20 mmol) were suspended in pyridine (1.6 mL) at room temperature, and the mixture was heated at 100°C for 3 h. After being cooled to 0°C, the reaction mixture was neutralized with 2 N aqueous HCl solution. The precipitate was then collected by filtration, and washed with cold water and n-hexane, which was further purified by recrystallization from ethyl acetate and n-hexane to give 1a (1.96 g, 50%) as a white solid.
ally prepared by following the general procedure as indicated below.
General Procedure for the Preparation of α,β-Unsaturated Carboxylic Acids (Procedure B1)
To a stirred solution of benzyloxyacetaldehyde (3.0 mmol) in toluene (15 mL) was added tert-butyl 2-(triphenylphosphoranylidene) acetate (1.7 g, 4.5 mmol). After being stirred at room temperature for 6 h, the reaction mix-ture was concentrated in vacuo. The residue was purified by column chromatography on silica gel (n-hexane–ethyl ace-tate=100 : 1 to 20 : 1) to afford E-1h-[OtBu] (530 mg, 81%) as a colorless oil, along with Z-1h-[OtBu] (44 mg, 7%).
General Procedure for the Preparation of α,β-Unsaturated Carboxylic Acids (Procedure B2)
To a stirred solution of (E)-1h-[OtBu] (232 mg, 0.93 mmol) in dichloromethane (DCM) (2.0 mL) was added trifluoroace-tic acid (TFA) (2.0 mL) dropwise at 0°C. After being stirred for 3 h, the reaction mixture was concentrated in vacuo. The residue was purified by column chromatography on silica gel (n-hexane–ethyl acetate=10 : 1 to 2 : 1) to afford the cor-responding α,β-unsaturated acid (E)-1h as an off-white solid (156 mg, 87%).
Procedure for the Preparation of 1iTo a stirred solution of tert-butyl (E)-6-hydroxyhex-2-eno-
ate (186 mg, 1.0 mmol) in DCM (2.0 mL) were added benzoyl chloride (180 µL, 1.5 mmol) and pyridine (120 µL, 1.5 mmol) at 0°C. After being stirred at room temperature for 3 h, the reaction mixture was quenched with 1 N aqueous HCl solution, and extracted with ethyl acetate three times. The combined organic layer was washed with saturated aqueous NaHCO3 solution, dried over MgSO4, and concentrated in vacuo. The residue was purified by column chromatography on silica gel (n-hexane–ethyl acetate=20 : 1 to 10 : 1) to afford the (E)-1i-[OtBu] as a colorless oil (300 mg). Deprotection of tert-butyl ester was performed by following the general procedure B2 to give 1i (82.6 mg, 71%, in 2 steps) as a colorless oil.
Preparation of Hydroxylamine DerivativesO-Benzylhydroxylamine, O-(tert-butyldimethylsilyl) hy-
droxylamine, 1,1-diphenylhydrazine, and benzyl carbamate were commercially available. Other nucleophiles were pre-pared as indicated below.
Procedure for the Preparation of O-Benzhydryl hydroxyl-amine (S-2)
To a stirred solution of diphenylmethanol (921 mg, 5.0 mmol), 2-hydroxyisoindoline-1,3-dione (978 mg, 6.0 mmol), and triphenylphosphine (1.57 g, 6.0 mmol) in tetrahydro-furan (THF) (20 mL) was added diisopropyl azodicarboxylate (DIAD) (1.2 mL, 1.5 mmol) at 0°C. After being stirred at room temperature for 1 h, the reaction mixture was concentrated in vacuo. The produced precipitate was collected by filtration and successively washed with n-hexane to afford the prod-uct S-1 (850 mg) as white solid, which was used for the next step without further purification. To a stirred solution of S-1 (658 mg, ca. 2.0 mmol) in EtOH (10 mL) was added hydrazine monohydrate (200 µL, 4.0 mmol) at room temperature. After being stirred at the same temperature for 1 h, the reaction mixture was concentrated in vacuo. The residue was puri-fied by column chromatography on silica gel (n-hexane–ethyl acetate=20 : 1 to 10 : 1) to afford S-2 as a colorless oil (390 mg, 50%, in 2 steps). 1H-NMR (400 MHz, CDCl3) δ: 7.38–7.23 (m, 10H), 5.64 (br s, 1H), 5.43 (br s, 2H); 13C-NMR (100 MHz, CDCl3) δ: 140.9 (2C), 128.5 (4C), 127.7 (2C), 127.1 (4C), 88.6; IR (ATR): 2926 cm−1; HR-MS (ESI): Calcd for C13H14NO [M+H]+ 200.1070. Found 200.1067.
Procedure for the Preparation of O-Heptylhydroxylamine (S-4)
To a stirred solution of heptan-1-ol (580 mg, 5.0 mmol), 2-hydroxyisoindoline-1,3-dione (984 mg, 6.0 mmol), and tri-phenylphosphine (1.57 g, 6.0 mmol) in THF (20 mL) was added DIAD (1.2 mL, 1.5 mmol) at 0°C. After being stirred at room temperature for 1 h, the reaction mixture was concentrated in vacuo. The residue was purified by column chromatogra-phy on silica gel (n-hexane–ethyl acetate=10 : 1) to afford the product S-3 as a yellow oil (1.39 g). To a stirred solution of S-3 (523 mg, ca. 2.0 mmol) in DCM (10 mL) was added hy-drazine monohydrate (500 µL, 10 mmol) at room temperature. After being stirred at the same temperature for 2 h, the reac-tion mixture was washed with H2O, dried over MgSO4, and concentrated in vacuo. The residue was purified by column chromatography on silica gel (n-hexane–ethyl acetate=4 : 1 to 2 : 1) to afford S-4 as a colorless oil (171 mg, 65%, in 2 steps). 1H-NMR (400 MHz, CDCl3) δ: 5.34 (br s, 2H), 3.66 (t, J=6.7 Hz, 2H), 1.62–1.53 (m, 2H), 1.37–1.23 (m, 8H), 0.88 (t, J=7.0 Hz, 3H); 13C-NMR (100 MHz, CDCl3) δ: 7.62, 31.8, 29.2, 28.4, 25.9, 22.6, 14.1; IR (ATR): 2930 cm−1; HR-MS (ESI): Calcd for C7H18NO [M+H]+ 132.1383. Found 132.1385.
Preparation of Boronic Acid CatalystsCatalysts (6a–i) were generally prepared as indicated below.General Procedure for the Preparation of 6 (Procedure C)To a stirred solution of tert-butyl ((1R,2R)-2-aminocyclo-
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hexyl) carbamate (107 mg, 0.5 mmol) in DCM (5.0 mL) were added Na2SO4 (500 mg, 3.5 mmol) and 2-formylphenylboronic acid (113 mg, 0.75 mmol) at room temperature under argon atmosphere. The reaction mixture was stirred at room tem-perature for 3 h, before the addition of NaBH(OAc)3 (318 mg, 1.5 mmol) at the same temperature. After being stirred for 3 h, the reaction mixture was quenched with 1 N aqueous HCl solu-tion, and extracted with CHCl3 twice. The combined organic layer extracts were washed with saturated aqueous NaHCO3 solution and brine, dried over MgSO4, and concentrated in vacuo. The crude S-5 (159 mg) was used for the next step without further purification. To a solution of S-5 (159 mg, 0.5 mmol) in DCM (5.0 mL) was added 37% aqueous formal-dehyde solution (200 µL, 2.5 mmol) followed by NaBH(OAc)3 (212 mg, 1.0 mmol) at room temperature under argon atmo-sphere. After being stirred at the same temperature for 3 h, the reaction mixture was quenched with 1 N aqueous HCl solution (pH=ca. 1), and extracted with CHCl3 twice. The combined organic layer extracts were washed with saturated aqueous NaHCO3 solution (pH=ca. 6) and brine, dried over MgSO4, and concentrated in vacuo. The residue was purified by column chromatography on silica gel (NH; n-hexane–ethyl acetate=4 : 1 to ethyl acetate only) to afford 6a as a white amorphous (84.6 mg, 47%, in 2 steps).
By following the general procedure C, 6i was prepared from 1-((1R,2R)-2-aminocyclohexyl)-3-(4-nitrophenyl) thiourea (886 mg, 3.0 mmol) (MeOH was used as solvent instead of DCM due to insolubility), and purified by column chromatog-raphy on silica gel (NH; n-hexane–ethyl acetate=4 : 1 to ethyl acetate–methanol=7 : 3). Yellow amorphous (531 mg, 56%, in 2 steps); 1H-NMR (acetone-d6, 400 MHz) δ: 9.47 (br s, 2H), 7.99 (d, J=9.3 Hz, 2H), 7.85–7.75 (m, 3H), 7.65 (d, J=6.7 Hz, 1H), 7.30–7.17 (m, 3H), 4.57–4.43 (m, 1H), 3.71 (dd, J=12.8 Hz, 2H), 2.56 (ddd, J1=J2=11.3 Hz, J3=3.2 Hz, 1H), 2.20 (s, 3H), 2.11–1.97 (m, 2H), 1.75–1.65 (m, 1H), 1.60–1.50 (m, 1H), 1.38–0.90 (m, 4H), one O–H or N–H proton was not observed; 13C-NMR (acetone-d6, 100 MHz) δ: 180.7, 147.5, 143.3, 142.5, 136.5, 130.9, 130.7, 127.7, 124.8 (2C), 121.9 (2C), 62.5, 60.5, 55.2, 35.3, 33.2, 25.6, 25.5, 22.5 One carbon peak could not be observed; IR (ATR): 3267, 2936, 1596, 1329, 1261 cm−1; HR-MS (ESI): Calcd for C21H28BN4O4S [M+H]+ 443.1923. Found 443.1920; [α]D
22+22.3 (c 1.01, CHCl3).General Procedure for Asymmetric Aza-Michael Ad-
dition of α,β-Unsaturated Carboxylic Acids and Their Derivatization to Determine the Enantiomeric Excess
General Procedure (D)To a stirred suspension of α,β-unsaturated carboxylic acid
1 (0.1 mmol), boronic acid catalyst (0.01 mmol), and activated MS 4 Å (50 mg) in CCl4 (0.25 mL), was added hydroxylamine derivatives (0.1 mmol) in CCl4 (0.25 mL) at room temperature. The reaction mixture was stirred at the room temperature for 24 h. The reaction mixture was directly purified by flash chro-matography on silica gel (n-hexane–ethyl acetate=4 : 1 to ethyl acetate–methanol=7 : 3) to afford crude 2 as a colorless oil. To a crude solution of 2 in toluene/methanol (0.75 mL/0.25 mL) at 0°C, was added trimethylsilyldiazomethane (TMSCHN2) in Et2O (0.25 mL, 2.0 M, 0.5 mmol, 5.0 equiv). The reaction mix-ture was stirred at 0°C for 30 min, before being quenched with AcOH until yellow solution changed to be colorless. The reac-tion mixture was purified by flash chromatography on silica gel to afford methyl esters 2-[OMe]. When it was difficult to separate the enantiomers of methyl ester 2-[OMe] by chiral HPLC, 2-[OMe] was further modified by N-benzoylation as shown below.
General Procedure (E)To a stirred solution of 2-[OMe] (0.05 mmol) in THF
(1.0 mL) were added benzoyl chloride (23 µL, 0.20 mmol) and pyridine (12 µL, 0.15 mmol) at 0°C. After being stirred at room temperature for 3 h, the reaction mixture was quenched with 1 N aqueous HCl solution (pH=ca. 1), and extracted with ethyl acetate. The combined organic layer was washed with saturated aqueous NaHCO3 solution (pH=9) and brine,, dried over MgSO4, and concentrated in vacuo. The residue was purified by column chromatography on silica gel to afford N-benzoyl compound (2-[NBz, OMe]).
21−4.5 (c 0.99, CHCl3) for 90% ee. 2a-[OMe] was converted to the corresponding known compound S-6 and its absolute configu-ration was determined to be S (Chart S1 in the Supplementary Materials).
(S ) -N- (Benzyloxy)-3-((benzyloxy)amino)-5-phenyl-pentanamide (3a)
Methyl (S)-3-(2,2-Dibenzylhydrazinyl)butanoate (7-[OMe])To a stirred suspension of α,β-unsaturated carboxylic
acid 1b (8.7 mg, 0.1 mmol), boronic acid catalyst 6i (4.4 mg, 0.01 mmol), and activated MS 4 Å (50 mg) in CCl4 (0.25 mL) was added 1,1-diphenylhydrazine (21.6 mg, 0.1 mmol) in CCl4 (0.25 mL) at room temperature. The reaction mixture was stirred at the room temperature for 24 h. The reaction mixture was directly purified by flash chromatography on silica gel (n-hexane–ethyl acetate=4 : 1 to ethyl acetate–methanol=7 : 3) to afford crude as a colorless oil. To the solution of the crude in toluene/methanol (0.75 mL/0.25 mL) at 0°C, was added TMSCHN2 in Et2O (0.25 mL, 2.0 M, 0.5 mmol). The reaction mixture was stirred at 0°C for 30 min, before being quenched with AcOH until yellow solution changed to be colorless. The reaction mixture was purified by flash chromatography on silica gel (n-hexane–ethyl acetate=10 : 1 to 7 : 1) to afford a colorless oil (28.4 mg, 91, 87% ee with cat. 6i); 1H-NMR (400 MHz, CDCl3) δ: 7.37–7.22 (m, 10H), 3.70 (d, J=13.0 Hz, 2H), 3.65 (d, J=13.0 Hz, 2H), 3.64 (s, 3H), 3.30–3.21 (m, 1H), 2.60 (br s, 1H), 2.51 (dd, J1=15.4 Hz, J2=6.1 Hz, 1H), 2.05 (dd, J1=15.4 Hz, J2=6.7 Hz, 1H), 0.95 (d, J=6.4 Hz, 3H); 13C-NMR (100 MHz, CDCl3) δ: 173.2, 137.8 (2C), 129.6 (4C), 128.1 (4C), 127.0 (2C), 60.3 (2C), 51.3, 49.3, 40.3, 19.2; IR (ATR): 2927, 1735 cm−1; HR-MS (ESI): Calcd for C19H25N2O2 [M+H]+ 313.1911. Found 313.1915; HPLC [Chiralcel OD-H, n-hexane–2-propanol=99 : 1, 1.0 mL/min, λ=254 nm, reten-tion times: (major) 9.6 min (minor) 7.9 min]; [α]D
To a stirred suspension of α,β-unsaturated carboxylic acid 1b (86 mg, 1.0 mmol), boronic acid catalyst 6i (89 mg, 0.2 mmol), and activated MS 4 Å (500 mg) in CCl4 (2.5 mL) was added benzyl carbamate (167 mg, 0.1 mmol) in CCl4 (2.5 mL) at room temperature. The reaction mixture was stirred at the room temperature for 24 h. The reaction mixture was purified by flash chromatography on silica gel (n-hexane–ethyl acetate=4 : 1 to ethyl acetate–methanol=7 : 3) to afford crude 2 as a colorless oil. To the solution of the crude 2 in toluene/methanol (7.5/2.5 mL) at 0°C, was added TMSCHN2 in Et2O (2.5 mL, 2.0 M, 5.0 mmol, 5.0 equiv.). The reaction mixture was stirred at 0°C for 30 min, before being quenched with AcOH until yellow solution changed to be colorless. The reaction mixture was purified by flash chromatography on silica gel (n-hexane–ethyl acetate=4 : 1 to 2 : 1) to afford a colorless oil (200 mg, 75, 86% ee with cat. 6i); 1H-NMR (400 MHz, CDCl3) δ: 7.41–7.30 (m, 5H), 6.29 (br s, 1H), 5.14 (s, 2H), 4.30 (br s, 1H), 3.66 (s, 3H), 3.53–3.43 (m, 1H), 2.47 (dd, J1=15.4 Hz, J2=6.7 Hz, 1H), 2.34 (dd, J1=15.4 Hz, J2=6.1 Hz, 1H), 1.11 (d, J=6.4 Hz, 3H); 13C-NMR (100 MHz, CDCl3) δ: 172.4, 157.3, 135.9, 128.6 (2C), 128.3, 128.2 (2C),
716 Vol. 64, No. 7 (2016)Chem. Pharm. Bull.
67.2, 52.4, 51.6, 39.5, 18.5; IR (ATR): 3304, 1726, 1261 cm−1; HR-MS (ESI): Calcd for C13H19N2O4 [M+H]+ 267.1339. Found 267.1339; HPLC [Chiralcel IC, n-hexane–2-propanol=90 : 10, 1.0 mL/min, λ=254 nm, retention times: (major) 30.6 min (minor) 27.4 min]; [α]D
22−7.3 (c 0.99 CHCl3) for 86% ee. 8-[OMe] was converted to the corresponding known compound S-8 and its absolute configuration was determined to be S (Chart S2 in the Supplementary Materials).
(E)-Tetradec-2-enoic Acid (9)To a stirred suspension of the dodecanal (370 mg, 2.0 mmol)
and malonic acid (210 mg, 2.0 mmol) in pyridine (0.5 mL), was added piperidine (20 µL) at room temperature. The mix-ture was heated at 90°C for 3 h. After being cooled to 0°C, the reaction mixture was neutralized with aqueous 2 N HCl solution (pH=ca. 1), and extracted with ethyl acetate. The combined organic layer was washed with brine, dried over MgSO4, and concentrated in vacuo. The residue was puri-fied by column chromatography on silica gel (n-hexane–ethyl acetate=10 : 1 to 4 : 1) to afford 9 as a colorless solid (402 mg, 89, 95% α,β-isomer; >99% E-isomer), which was further puri-fied by recrystallization from ethyl acetate/n-hexane at 0°C. mp 36.5–36.8°C (ethyl acetate–n-hexane); 1H-NMR (400 MHz, CDCl3) δ: 8.86 (br s, 1H), 7.09 (dd, J1=15.7 Hz, J2=7.0 Hz, 1H), 5.82 (dd, J=15.7 Hz, 1H), 2.23 (q, J=7.0 Hz, 2H), 1.51–1.42 (m, 2H), 1.36–1.22 (m, 16H), 0.88 (t, J=6.7 Hz, 3H); 13C-NMR (100 MHz, CDCl3) δ: 172.0, 152.6, 120.5, 32.3, 31.9, 29.61, 29.59, 29.50, 29.4, 29.3, 29.1, 27.8, 22.7, 14.1; IR (ATR): 2915, 1694 cm−1; HR-MS (ESI): Calcd for C14H25O2 [M−H]+ 225.1860. Found 225.1859.
(S)-3-Aminotetradecanoic Acid (10)To a stirred suspension of α,β-unsaturated carboxylic
acid 9 (22.7 mg, 0.1 mmol), boronic acid catalyst 1i (4.4 mg, 0.01 mmol), and activated MS 4 Å (50 mg) in CCl4 (0.25 mL) was added O-benzylhydroxylamine (12.4 mg, 0.1 mmol) in CCl4 (0.25 mL) at room temperature. The reaction mixture was stirred at the room temperature for 24 h. The reaction mixture was directly purified by flash chromatography on silica gel (n-hexane–ethyl acetate=4 : 1 to ethyl acetate–methanol=7 : 3) to afford crude adduct (37.9 mg) as a colorless oil. To this crude solution in methanol (5.0 mL) was added 10% Pd/C (30 mg), and then the mixture was subjected to an atmosphere of hy-drogen gas (300 kPa) for 24 h. The catalyst was filtered off and the solvent was evaporated to give the crude aminoacid 10, which was purified by column chromatography on silica gel (ethyl acetate only to CHCl3–MeOH=6 : 4) and (NH; ethyl acetate only to CHCl3–MeOH=8 : 2) to afford 10 (17.2 mg, 71%, in 2 steps) as a colorless solid; mp 188.0–189.6°C (metha-nol : H2O); 1H-NMR (400 MHz, CD3OD) δ: 3.25–3.18 (m, 1H), 2.39 (dd, J1=16.8 Hz, J2=3.5 Hz, 1H), 2.18 (dd, J1=16.8 Hz, J2=9.3 Hz, 1H), 1.55–1.48 (m, 2H), 1.36–1.14 (m, 18H), 0.80 (t, J=6.7 Hz, 3H), one O–H and two N–H protons were not ob-served; 13C-NMR (100 MHz, CDCl3) δ: 177.9, 50.9, 39.2, 34.1, 33.0, 30.70, 30.68, 30.59, 30.45, 30.43, 30.42, 26.4, 23.7, 14.4; IR (ATR): 2919, 2850, 1565, 1391 cm−1; HR-MS (ESI): Calcd for C14H30NO2 [M+H]+ 244.2271. Found 244.2273; [α]D
28+12.5 (c 0.83 CH3OH) for 90% ee (Lit32: [α]D
25+16.0 (c 0.90, H2O) for (S) enantiomer).
Methyl (S)-3-Benzamidotetradecanoate (10-[NBz, OMe])To a solution of 10 (12.1 mg, 0.05 mmol) in toluene/metha-
nol (0.75 mL/0.25 mL) at 0°C, was added TMSCHN2 in Et2O (125 µL, 0.25 mmol). The reaction mixture was stirred at 0°C
for 30 min, before being quenched with AcOH until yellow solution changed to be colorless. The reaction mixture was concentrated in vacuo. The crude ester (15 mg) was used for the next step without further purification. To a stirred solution of crude ester (15 mg) in THF (1.0 mL) were added benzoyl chloride (23 µL, 0.20 mmol) and pyridine (12 µL, 0.15 mmol) at 0°C. After being stirred at room temperature for 3 h, the reaction mixture was quenched with aqueous 1 N HCl solu-tion (pH=ca. 1), and extracted with ethyl acetate. The organic layer was washed with saturated aqueous NaHCO3 solution (pH=9), dried over MgSO4, and concentrated in vacuo. The residue was purified by column chromatography on silica gel (n-hexane–ethyl acetate=10 : 1 to 2 : 1) to afford a white solid (16.0 mg, 89%, in 2 steps); mp 95.3–95.5°C (ethyl acetate–n-hexane); 1H-NMR (400 MHz, CDCl3) δ: 7.79 (d, J=7.2 Hz, 2H), 7.50 (dd, J1=J2=7.2 Hz, 1H), 7.44 (dd, J1=J2=7.2 Hz, 2H), 6.90 (br d, J=9.3 Hz, 1H), 4.49–4.38 (m, 1H), 3.71 (s, 3H), 2.70 (dd, J1=15.9 Hz, J2=4.8 Hz, 1H), 2.63 (dd, J1=15.9 Hz, J2=4.6 Hz, 1H), 1.73–1.53 (m, 2H), 1.43–1.21 (m, 18H), 0.87 (t, J=6.7 Hz, 3H); 13C-NMR (100 MHz, CDCl3) δ: 172.7, 166.7, 134.6, 131.4, 128.5 (2C), 126.9 (2C), 51.7, 46.3, 38.0, 34.2, 31.9, 29.60, 29.59, 29.53, 29.47, 29.36, 29.31, 26.3, 22.7, 14.1; IR (ATR): 3298, 2921, 1736, 1639 cm−1; HR-MS (ESI): Calcd for C22H36NO3 [M+H]+ 362.2690. Found 362.2690; HPLC [Chi-ralcel IA, n-hexane–2-propanol=95 : 5, 1.0 mL/min, λ=254 nm, retention times: (major) 10.3 min (minor) 12.3 min]; [α]D
20−31.5 (c 1.02 CHCl3) for 90% ee.
(S)-1-(Benzyloxy)-5-phenethylimidazolidin-2-one (11)To a stirred suspension of α,β-unsaturated carboxylic
acid 1a (17.7 mg, 0.1 mmol), boronic acid catalyst 6i (4.6 mg, 0.01 mmol), and activated MS 4 Å (50 mg) in CCl4 (0.25 mL) was added a solution of O-benzylhydroxylamine (12.4 mg, 0.1 mmol) in CCl4 (0.25 mL) at room temperature. The reac-tion mixture was stirred at the room temperature for 24 h, before the successive addition of O-diphenylphosphoryl azide (32 µL, 0.15 mmol) and N,N-diisopropylethylamine (25 µL, 0.15 mmol) at room temperature. The reaction mixture was heated at 50°C for 3 h. Then, the reaction mixture was directly purified by flash chromatography on silica gel (n-hexane–ethyl acetate=4 : 1 to ethyl acetate only) to afford the product 11 as a colorless oil (20.2 mg, 68, 89% ee). 1H-NMR (400 MHz, CDCl3) δ: 7.44 (dd, J1=7.5 Hz, J2=1.4 Hz, 2H), 7.37–7.24 (m, 5H), 7.23–7.17 (m, 1H), 7.11 (d, J=7.2 Hz, 2H), 5.60 (s, 1H), 5.10 (d, J=10.7 Hz, 1H), 4.92 (d, J=10.7 Hz, 1H), 3.61–3.50 (m, 1H), 3.32 (dd, J1=J2=8.1 Hz, 1H), 2.98 (dd, J1=J2=9.0 Hz, 1H), 2.58 (t, J=8.1 Hz, 2H), 2.10–1.99 (m, 1H), 1.77–1.64 (m, 1H); 13C-NMR (100 MHz, CDCl3) δ: 164.4, 140.8, 136.0, 129.5 (2C), 128.5 (2C), 128.43, 128.35 (2C), 128.2 (2C), 126.1, 78.6, 60.5, 42.2, 32.9, 31.3; IR (ATR): 1735 cm−1; HR-MS (ESI): Calcd for C18H21N2O2 [M+H]+ 297.1598. Found 297.1596; HPLC [Chiral-cel IB, n-hexane–2-propanol=90 : 10, 1.0 mL/min, λ=254 nm, retention times: (major) 13.9 min (minor) 16.3 min]; [α]D
25+69.4 (c 0.98, CHCl3) for 89% ee.
Acknowledgment This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas “Advanced Molecular Transformations by Organocatalysts” from the Ministry of Education, Culture, Sports, Science and Technol-ogy (MEXT) of Japan (23105007 to Y.T.).
Conflict of Interest The authors declare no conflict of
Vol. 64, No. 7 (2016) 717Chem. Pharm. Bull.
interest.
Supplementary Materials The online version of this ar-ticle contains supplementary materials.
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