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Essential for
Improved Sample Preparation:
Strong bases •strata-X-CW, p.12Acids •strata-X-AW, p.13On-line extraction cartridge •Strata C18 20 x 2.0 mm, p.17
For Traditional Sample Preparation For High-Throughput Screening
For On-line Screening† For Flash Analysis For Flash and Large Scale-Up
†strata-X now available in on-line formatSee p. 17 for ordering information
For Protein Precipitation
Strata is a registered trademark of Phenomenex, Inc. strata-X is a trademark of Phenomenex, Inc.
STRATA® SPE (SOLID PHASE EXTRACTION)If Strata SPE or Sepra bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!
�Phenomenex
SOLID PHASEEXTRACTION
Strata
So
lidP
haseExtractio
n
Materialcharacteristics Particle Size Pore Size Surface Area Carbon Load Bonding End Capping Ionic Capacity Phase (µm) (Å) (m2/g) (%) (meq/g)
**Recommended Alternative - This category indicates an alternative phase which may yield somewhat different selectivity but may also lead to improved resolution.
PhaseSelectionbyManufacturerPhenomenex Waters Varian Supelco JT BAKER IST Macherey-Nagel STRATA ** Sep-Pak Bond Elut Discovery UCT Bakerbond ISOLUTE Chromabond
SPE sorbents are most commonly categorized by the nature of their primary interaction or retention mechanism with the analyte of interest. The three most common extraction mechanisms used in SPE are reversed phase, normal phase and ion exchange.
This concise diagram is designed to assist you in selecting the proper sorbent for your application. For more detailed information, reference books are available (page 372).
SelectingTheProperPhase
Mechanism TypicalApplication
reversedPhase Extraction of hydrophobic or polar organic analytes from aqueous matrix
normalPhase Extraction of polar analytes from non-polar organic solvents
IonExchange Extraction of charged analytes from aqueous or non-polar organic samples
aqueous
water
ionic
organic
organic
neutral
organic
neutral
nonpolar
reversedphase
strata-X
SDB-L
C18
C8
PH
(+)basic
cationexchange
strata-X-C
SCX
WCX
Screen-C
Screen-C GF
(-)acidic
anionexchange
SAX
NH2
Screen-A
polar**
reversedphase*
strata-X
CN
neutral
moderately**polar
reversedphase
strata-X
C8
CN
non**polar
reversedphase
strata-X
SDB-L
C18
C8
PH
moderatelypolar
normalphase
Si-1
NH2
polar
normalphase
CN
Si-1
FL-PR
NH2
(+)basic
cationexchange
strata-X-C
SCX
WCX
Screen-C
Screen-C GF
(-)acidic
anionexchange
SAX
NH2
Screen-A
ionic
strata-X-AW
strata-X-CW strata-X-CW
strata-X-AW
*NOTE: These can be difficult to extract. Consider non-retentive reversed-phase to remove interference.
Polarity logP
Polar log P<1
Mid-Polar(moderately) log P=1-3
non-Polar log P>3
log P is the partition coefficient that can be used to determine the polarity of a compound
**
Str
ata
Met
hod
Dev
elo
pm
ent
STRATA® SPE METHOD DEVELOPMENT
For the latest updates in SPE method development, technical support, and troubleshooting, visit www.strataSPE.com
The sorbent bed mass determines the maximum amount of analyte that can be retained by the sorbent.
GeneralruletoDetermineProperBedMass
Silica-basedsorbents Non-ionic sorbents retain a mass of solute (analyte plus retained contaminants) that is equivalent to approximately 5 % of the sorbent mass. Therefore, a 100 mg cartridge can retain approximately 5 mg of total solute mass.
Polymericsorbents The large surface area of polymers give a higher capacity that is approximately 10-15 % of the sorbent mass.
Ionexchangesorbents Capacity is determined by the number of charged sites on the sorbent available to interact with the analyte. The mean capacity ranges from 0.8-1.3 meq/ g as shown on page 5.
For a compound to be extracted in a SPE method, the molecule must not be too large to fit into the pores. For large molecules, sorbents with extra large pores must be used. The average pore size for the Strata sorbents is given on page 5.
Reduce breakthrough by selecting the proper bed mass!
Since the majority of SPE is performed on relatively “dirty” samples that contain many potential contaminants, it is common practice to be conservative and choose a slightly larger sorbent mass.
The bed volume of the sorbent determines the solvent volumes required in a SPE method. In general, solvent volumes between 4 to 8 times the bed volume are typically necessary to ensure proper conditioning, washing and elution. Conditioning with less than 4 to 8 bed volumes increases the risk of incomplete solvation of the bed and low or irreproducible recoveries, while more than 4 bed volumes is typically unnecessary.
Conventional packed bed, silica-based SPE products typically have a bed volume of approximately 150 µL per 100 mg of sorbent. Polymeric sorbents require larger solvent volumes for proper conditioning, washing and eluting. It is recommended to use 200-250 µL per 100 mg of sorbent.
For the latest updates in SPE method development, technical support, and troubleshooting, visit www.strataSPE.com
OrDErInGInFOrMATIOnSample Preparation Method Development KitsPart No. Description Unit Pricestrata-X Method Development Kit for Sample Cleanup and Concentration
KS0-7908 strata-X 200 mg/3 mL tubes (10 ea) I ea I strata-X-C 200 mg/3 mL tubes (10 ea) strata-X-CW 200 mg/3 mL tubes (10 ea) strata-X-AW 200 mg/3 mL tubes (10 ea) Strata Method Development Demo Softwarestrata-X Method Development 96-Well Plate Kit for High-Throughput Screening
KS0-8241 strata-X 10 mg/well (3 rows) I ea I strata-X-C 10 mg/well (3 rows) strata-X-CW 10 mg/well (3 rows) strata-X-AW 10 mg/well (3 rows)
KS0-8209 strata-X 30 mg/well (3 rows) ea strata-X-C 30 mg/well (3 rows) strata-X-CW 30 mg/well (3 rows) strata-X-AW 30 mg/well (3 rows) Strata Starter Kit for Protein Precipitation
CE0-8201 Strata Impact Protein Precipitation Plate (2 ea) I ea I Collection Plate 2 mL (2 ea) Sealing Mat (2 ea)
Polarandnon-PolarCondition: With methanolEquilibrate: With waterLoad Sample: Phosphoric Acid (2 % of total
volume) can be used to disrupt drug and protein interaction
Wash: With 5-60 % methanol in water (organic concentration used in
wash can vary based on nature of analyte) Dry for 1 minute
Elute Analyte: With methanol/acetonitrile (50:50)
strata-X
reversedPhase
Bases Condition: With methanolEquilibrate: With waterLoad Sample: Acidified with 2 % Phosphoric
AcidWash 1: With 0.1 N HCIWash 2: With methanol (elutes acidic
and neutral analytes) This helps in removing endogenous contaminants from biological sample matrices, and eliminates ion suppression in LC/MS under ESI mode.
Elute Analyte: With 5 % ammonium hydroxide/methanol* or acetonitrile
StrongBases Condition: With methanolEquilibrate: With waterLoad Sample: pH of sample should be within
4.5-7.0*Wash 1: With 25 mM ammonium
acetate buffer pH 6.5Wash 2: With methanol (elutes acidic
and neutral analytes) This serves to remove
endogenous contaminants from biological sample matrices, and eliminates ion suppression. Dry for 1 minute
Elute Analyte: With 2 % formic acid in methanol/acetonitrile (20:80)
Depending on analyte solubility and hydrophobicity, isopropanol/methylene chloride (20:80) can be substituted for methanol/acetonitrile
* Samples may need to be acidified to disrupt drug-protein interaction. Also sample should be diluted 1:1 with buffer or water ensuring that sample is two pH units below pKa of analyte and two pH units above the pKa of the sorbent.
StrongAcids Condition: With methanolEquilibrate: With waterLoad Sample: pH of sample should be 2
pH units above the pKa of the acidic analyte to ensure that the analyte is fully deprotonated
Wash 1: With waterWash 2: With methanol (elutes basic
and neutral analytes)Elute: 2 % NH4OH in methanol
N..
n)
)
O n)
)O-S
O
O
n)
)O
OH
n)) NH NH2
* For very hydrophobic compounds methanol can be substituted with methylene chloride and/or isopropanol.
* Modified conditioning step(s) such as acidifying the methanol (2 % formic) may be needed to improve selectivity.
†The above is a convenient starting point for SPE method development. Further optimization may be required to tailor the method to your specific needs.
EliminatesWell-to-WellVariabilityMost traditional silica-based sorbents will lose their extraction ability if the sorbent dries or becomes ‘deconditioned’. strata-X remains conditioned, yielding extremely high and consistent recoveries, even if the sorbent runs dry!
This is a great feature because it eliminates the possibility of well-to-well variability most commonly associated with silica-based 96-well plates.
conditions:Tubes: 30 mg/1 mL strata-X, 100 mg/1 mL Strata C18-ESamples: Analytes spiked in phosphate buffer saline solution (PBS) at concentration of 1 µg/ mL, n=3Method: The strata-X method was used. After the conditioning step the sorbent was dried under vacuum from 0 up to 10 min at 10" Hg. Percent recoveries were determined by HPLC analysis.
HighAnalytecapacity33 µm strata-X particles have a very high surface area that results in extremely high analyte capacity. strata-X will retain an analyte mass (including potential interference) that is equivalent to 6-15 % of the sorbent bed mass (i.e., a 200 mg cartridge retains approximately 20 mg of total solute mass).
ShorterDryDownTimesThe high analyte capacity of strata-X means that you will need a smaller bed mass which results in lower elution volumes for shorter dry down times and fast sample cleanup.
Ion suppression study performed with post column infusion of Atenolol, positive ESI mode m/z 267. Contact Phenomenex for additional method details. Captiva® Protein precipitation plate is a registered trademark of Varian Inc. Phenomenex is in no way affiliated with Varian. May not be representative of all SPE applications shown.
2 µg/mL acrylamide in 0.2 g French friesStreamlined method development
2.92
2 -
Acr
ylam
ide
Ansys Captiva® Protein Precipitation Plate
strata-X-C
Intens.
x10
2.0
1.5
1.0
0.5
0 1 2 3 4 5 6 min
SingleStepcleanupofAcrylamidefromFriedPotatoes
0
10
20
30
40
50
60
70
80
90
100
% R
ecov
ery
>78 % ≥84 % >91 %
Acrylamide
500
ng/m
L
50 n
g/m
L
2000
ng/
mL
TN-0
07
Sorbent: strata-X-C 200 mg/3 mL Part No.: 8B-S029-FBJCondition: 2 x 1 mL methanolEquilibrate: 2 x 1 mL water Load: 1 mL homogenized supernatant, dry for 30 secElute: 1 mL water at a flow rate of 0.5 mL/ minEvaporate: remove excess water
Plasma Extracts (LC/MS extracted ions m/z; 304(C12) and 332(C14))
+ -
+ -
EIC 332 All + -
1 2 3 4 5 6 7 0
EIC 304 All
TIC All Elute Fraction
+ -
+-
EIC 332 All + -
0
20
40
60
80
100
% R
ecov
ery
Clid
iniu
m B
rom
ide
ExtractionofBenzalkoniumchloridefromPlasmaSorbent: strata-X-CW 30 mg/1 mLPart No.: 8B-S035-TAKCondition: 1 mL methanol Equilibrate: 1 mL waterLoad: Analyte spiked human plasma (350 µL plasma + 700 µL water and acidify with 20 µL phosphoric acid)Wash 1: 1 mL 25 mM sodium acetate buffer pH 4.5, Dry for 1 minWash 2: 1 mL methanolElute: 1 mL 5 % formic acid/methanol Inject the elution fraction onto the LC-MS (no need to dry down)
ExtractionofclidiniumBromidefromPlasmaSorbent: strata-X-CW 30 mg/1 mLPart No.: 8B-S035-TAKCondition: 1 mL methanol Equilibrate: 1 mL waterLoad: Analyte spiked human plasma (350 µL plasma + 700 µL water and acidify with 20 µL phosphoric acid)Wash 1: 1 mL 25 mM sodium acetate buffer pH 4.5, Dry for 1 minWash 2: 1 mL methanolElute: 1 mL 5 % formic acid/methanol Inject the elution fraction onto the LC/MS (no need to dry down)
Adenosine Sorbent: strata-X-AW 30 mg/1 mLPart No.: 8B-S038-TAKCondition 1: 1 mL methanol Condition 2: 1 mL formic acid / methanol / water (2:25:73)Equilibrate: 1 mL waterLoad: 1 mL 0.2 mg/mL in phosphate buffered saline / water (1:1) Wash 1: 1 mL waterWash 2: 1 mL methanol / water (50:50)Elute: 1 mL NH4OH / methanol / water (2:25:78) Elution fraction 1:1 with 25 % methanol / 20 mM NH4 acetate, pH 5.3 prior to injection (no need to dry down or reconstitution)
ExtractionofOrtho-Phosphotyrosine Sorbent: strata-X-AW 30 mg/1 mLPart No.: 8B-S038-TAKCondition 1: 1 mL methanol Condition 2: 1 mL formic acid / methanol / water (2:25:73)Equilibrate: 1 mL waterLoad: 1 mL 200 µg/mL in phosphate buffered saline / water (1:3)Wash 1: 1 mL waterWash 2: 1 mL methanol / water (50:50)Elute: 1 mL NH4OH / methanol / water (5:50:45) Elution fraction 1:1 with water prior to injection (no need to dry down or reconstitute)
OrDErInGInFOrMATIOn Note: Tube sizes not proportional nor actual size.
If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!
1�Phenomenex
SOLID PHASEEXTRACTION
Strata
Gig
aTubes
STRATA® GIGA™ TUBES
�00 mg 1 g 1 g 12 mL 12 mL 20 mL (20/box) (20/box) (20/box)
75 mg 150 mg 150 mg
10 mL 20 mL 20 mL
�00 mg 2 g � g 10 g 20 g 12 mL 12 mL 20 mL 60 mL 60 mL (20/box) (20/box) (20/box) (16/box) (16/box)
† Retention capacities and elution volumes are specific to the chemical nature of the analyte being extracted, its concentration in the sample, the chemical nature of the eluting solvent and the bed mass used. The above are only guidelines. An elution study should be conducted. As a general rule, recommended solvent wash and elution volumes for silica-based material should be 1.2 mL per 100 mg of sorbent and 2 mL per 100 mg of polymeric sorbent,
** Teflon-coated tubes available, contact Phenomenex for details.
Note: Tube sizes not proportional nor actual size.
If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!
1� Phenomenex
SOLI
D PH
ASE
EXTR
ACTI
ONS
trat
aH
igh-
Thr
oug
hput
Pla
tes
Contact Phenomenex or your local Phenomenex distributor for latest 96-well plate technical note.
CE0-7565 I Strata Impact Square Well Plate, 2 mL I 2 I
OrDErInGInFOrMATIOn(2PlATES/BOX)
2 Plates/Box
See p. 8 for Strata-X Method Development Kits
See p. 8 for Strata Starter Kit for Protein Precipitation
If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!
1�Phenomenex
SOLID PHASEEXTRACTION
Strata
Hig
h-Thro
ughp
utAccesso
ries&O
n-linecartrid
ges
HandbookofBioanalysisandDrugMetabolismEdited by Gary Evans
High recoveries by strong cationexchange retention mechanism
B
A
Barbituratesfromurine
CN-0
14CN
-005
Met
hado
ne
Phen
obar
bita
l
Buta
lbita
l
Amob
arbi
tal
ExtremelycleanExtracts
HighrecoveriesforDrugsofAbuse
Reversed Phase
Mixed Mode Cation Exchange
Mixed Mode Cation Exchange
Sorbent: strata-X 30 mg/1 mL Part No.: 8B-S100-TAK Condition: 1 mL methanol Equilibrate: 1 mL water Load: 1 mL porcine spiked with analyte Wash: 1 mL 5 % methanol in water Elute: 1 mL methanol Evaporate: add 25 µL of 0.1 mg/mL butyl paraben (external standard) dry down under slow stream of N2 and reconstitute in 200 µL acetonitrile
HPLC Column: Synergi™ Max-RP 4 µm 150 x 4.6 mm SecurityGuard™ C18 4 x 3.0 mm Part No.: 00F-4337-E0 and AJ0-4287 Sample: 50 µL of reconstituted extract Mobile Phase: A: 20 mM KH2PO4 (pH 7.0) B: methanol A/B (35:65) for 20 min Flow Rate: 1.0 mL/min Temperature: 25 °C
Detector: UV @ 254 nm Peaks: 1. Prednisolone 2. Betamethasone 3. Butyl Paraben (external std) 4. Betamethasone Valerate Chromatogram of Extracts: A) Blank B) Spiked Sample
Sorbent: strata-X-C 60 mg/3 mL Part No.: 8B-S029-UBJCondition: 2 mL methanolEquilibrate: 2 mL waterLoad: 3 mL urine spiked with analytes and IS (0.5 µg/mL); dilute 1:1 with 100 mM KH2PO4, pH 6; acidified with 2 % phosphoric acidWash 1: 2 mL 0.1 N HCIWash 2: 2 mL methanolElute: 2 mL 5 % NH4OH/methanolEvaporate: dry down and reconstitute with 200 µL mobile phase
HPLC Column: Synergi™ Max-RP 4 μm 150 x 4.6 mmPart No.: 00F-4337-E0Sample: 50 µL of reconstituted urine extractMobile Phase: A: 0.1 % TFA in water, pH 2.1 B: acetonitrite A/B (65:35)Flow Rate: 1.0 mL/minTemperature: 25 °C
Detector: UV @ 210 nmPeaks: 1. Estazolam (IS) 2. MethadoneChromatogram of Extracts: A) Blank B) Spiked Sample
Sorbent: strata-X-C 60 mg/3 mL Part No.: 8B-S029-UBJCondition: 2 mL methanolEquilibrate: 2 mL waterLoad: 2 mL human urine spiked with analytes and IS (0.5 µg/mL); dilute 1:1 with water Wash 1: 2 mL 5 % methanol in waterWash 2: 2 mL 10 % methanol in 2 % acetic acid and dry for 1 minElute: 2 mL 35 % methanol/2 % NH4OHEvaporate: dry down under slow stream of N2 and reconstitute in 300 µL mobile phase
HPLC Column: Synergi™ Max-RP 4 μm 150 x 4.6 mmPart No.: 00F-4337-E0Sample: 50 µL of reconstituted urine extractMobile Phase: A: 20 mM KH2PO4, pH 7.0 B: acetonitrile Gradient: A/B: (80:20) hold 3 min to (50:50) in 12 min Flow Rate: 1.0 mL/minTemperature: 25 °C
Detector: UV @ 214 nmPeaks: 1. Phenobarbital 3. Amobarbital 2. Butalbital 4. Secobarbital (IS)Chromatogram of Extracts: A) Blank B) Spiked Sample
GC Analysis ConditionsGC Column: Zebron™ ZB-5ms Part No.: 7HM-G010-11Dimensions: 30 meter x 0.32 mm x 0.25 µmInjection: 2 µL splitless @ 285 °CCarrier Gas: Helium @ 3 mL/min Oven Program: 60 °C for 1 minute then ramp to 290 °C at 8 °C/min hold for 6.75 minutes Detector: FID @ 315 °C
Sample Preparation: Solid Phase Extraction ConditionsSorbent: Strata® EPH 5 g/20 mLPart No.: 8B-S031-LEGEquilibrate: 30 mL HexaneLoad: SampleElute Aliphatics C9-C18 and C19-C36: 11 mL Hexane (Lower elution volume allows for more concentrated fraction and faster sample dry down times)Elute Aromatics C11-C22: 20 mL Methylene ChlorideConcentrate: Samples to 1 mL
Interested in MSPD for your analysis? Please contact us for technique and accessory information.
Bondesil® is a registered trademark of Varian Inc. ISOLUTE® is a registered trademark of International Sorbent Technology Ltd. Florisil® is a registered trademark of U.S. Silica
Sepra high performance bulk SPE sorbents are excellent for applications such as MSPD (matrix solid phase dispersion) in which drugs of interest are separated from homogenized animal tissue or food sources. These high quality sorbents can also be used for flash analysis to effectively purify organic compounds.
OrDErInGInFOrMATIOn
If Sepra Bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Sepra Bulk for FREE!
21Phenomenex
SOLID PHASEEXTRACTION
3M EMPORE™
3ME
mp
ore
For fast and easy sample extraction, Phenomenex offers filtration glassware for Empore Extraction Disks. Our 47 mm all-glass microfiltration units are used for individual extractions and are described on page 350. Three- and six-station Extraction Manifolds are also available, please inquire.
Empore Extraction DisksPart No. Mfr. No. Description Disk Diameter (mm) Unit PriceAH0-2540 I 2215 I C18 I 47 I 20/pk I
AH0-2541 2315 C18 90 10/pk
AH0-2543 2214 C8 47 20/pk
AH0-3482 2252 Anion-Exchange 47 20/pk
AH0-3485 2240 SDB-XC 47 20/pk
AH0-4048 2241 SDB-RPS 47 20/pk
AH0-3488 2270 Oil and Grease 47 20/pk
Empore Disk CartridgesPart No. Mfr. No. Description Size* Unit PriceAH0-4056 I 4115 I C18-SD** I 4 mm/1 mL I 100/pk I
AH0-4057 4215 C18-SD*a* 7 mm/3 mL 50/pk
AH0-4058 4315 C18-SD** 10 mm/6 mL 30/pk
* Disk diameter/cartridge volume. ** SD= Standard Density is optimized for biological matrices (40 µm particle, enhanced flow characteristics).
Filtration GlasswarePart No. Description Disk Diameter (mm) Unit Price
Filtration Glassware Complete Assemblies I I I AH0-1566 FilterSys, Filtration Glassware, All-Glass, 300 mL funnel with 1 L flask 47 ea
AH0-3314 FilterSys, Filtration Glassware, All-Glass, 500 mL funnel with 2 L flask 47 ea
AH0-3315 FilterSys, Filtration Glassware, All-Glass,1000 mL funnel with 4 L flask 47 ea
Filtration Glassware Component Parts AH0-1567 Fritted Support Base, 40/35 Ground-Glass Joint 47 ea
AH0-1568 Funnel Reservoir, Graduated, 300 mL 47 ea
Protein Precipitation /Filter PlatePart No. Mfr. No. Description Unit PriceFilter Plate/Precipitation PlatesCE0-7203 6060 Filter Plate PPT, ea Standard 1.2 mL Capacity
CE0-7208 6360 Filter Plate PPT, ea Deep Well 2.5 mL Capacity
All 3M Filtration Products are available from Phenomenex. Call for availability. * 3M and Empore are trademarks of 3M.
See p. 16 for Strata Impact Protein Precipitation Plates.
Universal Resin Membrane Extraction Disk PlatesPart No. Mfr. No. Description Unit Price
CE0-7209 I 6045 (SD) I Universal Resin Plate, I ea I Standard 1.2 mL Capacity
CE0-7210 6345 (SD) Universal Resin Plate, ea Deep Well 2.5 mL Capacity
Disk Plates for Silica-Based ExtractionPart No. Mfr. No. Description Unit PriceStandard Well Extraction Disk Plates, 1.2 mL CapacityCE0-7199 6011 (SD) C2-SD (Ethyl) ea
CE0-7200 6014 (SD) C8-SD (Octyl) ea
CE0-7201 6015 (SD) C18-SD (Octadecyl) ea
CE0-7202 6030 (SD) MPC-SD (Mixed Phase Cation) ea
Deep Well Extraction Disk Plates, 2.5 mL CapacityCE0-7206 6315 (SD) C18-SD (Octadecyl) ea
OrDErInGInFOrMATIOn10 - Position Tall-Boy Vacuum Manifold*Part No. Description Unit Price
AH0-7502 I SPE 10-Position Tall-Boy Vacuum Manifold, complete assembly I ea I
AH0-7503 SPE 10-Position Tall-Boy Vacuum Manifold, Glass Chamber ea AH0-7504 SPE 10-Position Tall-Boy Vacuum Manifold, Cover, Gasket and 10 Stopcocks ea
12 – Position Vacuum Manifold*
AH0-6023 SPE 12-Position Vacuum Manifold Set, complete assembly ea
12 – Position Vacuum Manifold Replacement Parts
AH0-6025 SPE 12-Position Glass Chamber ea AH0-6027 SPE Cover, Gasket and 12 Stopcocks ea AH0-6029 SPE Gaskets 2/pk AH0-6031 SPE Vacuum Gauge, Valve and Glass Chamber ea AH0-6037 SPE Collection Rack, including shelves, legs, clips and posts ea AH0-6040 SPE Plate for 13 mm Test Tube ea AH0-6043 SPE Plate for 16 mm Test Tube ea AH0-6042 SPE Plate for Volumetric Flask ea AH0-6045 SPE Plate for Autosampler Vial ea AH0-6046 SPE Plate, Dimple ea AH0-6047 SPE Plate, Base ea AH0-6048 SPE Stopcocks 12/pk AH0-6052 SPE 12-Position Vacuum Waste Container, polypropylene 10/pk
24 – Position Vacuum Manifold*
AH0-6024 SPE 24-Position Vacuum Manifold Set, complete assembly ea
24 – Position Vacuum Manifold Replacement Parts
AH0-6026 SPE Glass Chamber ea AH0-6028 SPE Cover, Gasket and 24 Stopcocks ea AH0-6030 SPE Gaskets 2/pk AH0-6032 SPE Vacuum Gauge ea AH0-6038 SPE Collection Rack, including shelves, legs, clips and posts ea AH0-6041 SPE Plate for 13 mm Test Tube ea AH0-6044 SPE Plate for 16 mm Test Tube ea AH0-6049 SPE Stopcocks 24/pk
General Vacuum Manifold Accessories
AH0-6033 SPE Manifold Needles, polypropylene 12/pk AH0-6034 SPE Manifold Needles, polypropylene 24/pk AH0-6035 SPE Manifold Needles, stainless steel 12/pk AH0-6036 SPE Manifold Needles, stainless steel 24/pk AH0-6050 SPE Drying Attachment for 12-Position manifold ea AH0-6051 SPE Drying Attachment for 24-Position manifold ea AH0-6053 Female Luer Fittings 2/pk AH0-6054 Male Luer Fittings 2/pk AH0-6055 Support Posts for Rack 3/pk AH0-6056 Legs for Cover, black 4/pk AH0-6057 Vacuum Gauge and Valve Assembly ea AH0-6058 Valve Assembly Only ea AH0-6059 Vacuum Gauge Only ea AH0-6060 Retaining Clips 12/pk AH0-6061 Vacuum Manifold Plugs 50/pk AH0-6062 Control Valve, Teflon® 25/pk AH0-6063 Control Valve, Teflon® 50/pk AH0-6064 Teflon® Needles 100/pk AH0-6065 Teflon® Needles 500/pk AH0-7191 Adaptor Caps for 1, 3 and 6 mL SPE tubes, polyethylene, with Luer hole in the top 15/pk AH0-7378 Adaptor Caps for 12 and 20 mL SPE tubes, polyethylene, with Luer hole in the top 5/pk AH0-7379 Adaptor Caps for 60 mL SPE tubes, polyethylene, with Luer hole in the top 5/pk AH0-7907 Adaptor Caps for 150 mL SPE tubes, polyethylene, with Luer hole in the top ea
(1) The 10-position Tall Boy Vacuum Manifold Collection Rack includes 4 plates: one base plate, one dimple plate, one small plate and one large plate and three riser bar legs, along with 12 manifold clips to support the plates. The assembly also includes 10 polypropylene needles, 10 stopcocks and 4 black legs to support the lid when taken off the glass block.(2) The 12-position Collection Rack consists of 3 support posts, bottom plate, 13 mm plate, 16 mm plate, autosampler plate, volumetric plate, and 12 retaining clips. (3) The 24-position Collection Rack consists of 3 support posts, bottom plate, dimple plate, 13 mm plate, 16 mm plate, and 24 retaining clips.
* Manifolds include: Vacuum-tight glass chamber, polypropylene lid with gasket, bleed valve and gauge, stopcock valves, collections racks, polypropylene needles