Nature Methods Spatiotemporal control of gene expression by a light-switchable transgene system Xue Wang, Xianjun Chen & Yi Yang Supplementary Figure 1 Absorption spectrum of purified Gal4(65)-VVD Supplementary Figure 2 Schematic representation of LightON system components. Supplementary Figure 3 Comparison of induction levels of Fluc reporters driven by GAVV and the conventional CMV driven vectors. Supplementary Figure 4 Light induced expression of fluorescent proteins in HEK293 cells. Supplementary Figure 5 RT-PCR analysis of Fluc transcription. Supplementary Figure 6 Effect of duration of illumination on Gluc reporter expression. Supplementary Figure 7 Semilogorithmic plot of cellular Gluc mRNA dynamics. Supplementary Figure 8 Expression kinetics of the Gluc reporter in cell culture medium. Supplementary Figure 9 Quantitative control of gene expression in mammalian cells by modulating the light irradiance Supplementary Figure 10 Effect of light irradiance on Gluc expression induced by a single blue light pulse Supplementary Figure 11 Printing an image on a single layer of cultured cells by light induction of mCherry expression. Supplementary Figure 12 Spatial control of mCherry transgene expression in mice liver Supplementary Figure 13 LED arrays and laser devices used for light-switchable transgene expression Supplementary Figure 14 Full-length gels of Fig. 1b Supplementary Figure 15 Full-length blots of Fig. 1e Supplementary Table 1 Comparison of light-inducible gene expression methodologies. Supplementary Table 2 Primers for RNA analysis Supplementary Note Sequence information. Nature Methods: doi:10.1038/nmeth.1892
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Nature Methods
Spatiotemporal control of gene expression by a light-switchable
transgene system
Xue Wang, Xianjun Chen & Yi Yang
Supplementary Figure 1 Absorption spectrum of purified Gal4(65)-VVD Supplementary Figure 2 Schematic representation of LightON system components. Supplementary Figure 3 Comparison of induction levels of Fluc reporters driven
by GAVV and the conventional CMV driven vectors. Supplementary Figure 4 Light induced expression of fluorescent proteins in
HEK293 cells. Supplementary Figure 5 RT-PCR analysis of Fluc transcription. Supplementary Figure 6 Effect of duration of illumination on Gluc reporter
expression. Supplementary Figure 7 Semilogorithmic plot of cellular Gluc mRNA dynamics. Supplementary Figure 8 Expression kinetics of the Gluc reporter in cell culture
medium. Supplementary Figure 9 Quantitative control of gene expression in mammalian
cells by modulating the light irradiance Supplementary Figure 10 Effect of light irradiance on Gluc expression induced by a
single blue light pulse Supplementary Figure 11 Printing an image on a single layer of cultured cells by
light induction of mCherry expression. Supplementary Figure 12 Spatial control of mCherry transgene expression in mice
liver Supplementary Figure 13 LED arrays and laser devices used for light-switchable
transgene expression Supplementary Figure 14 Full-length gels of Fig. 1b Supplementary Figure 15 Full-length blots of Fig. 1e Supplementary Table 1 Comparison of light-inducible gene expression
CONSTRUCT: U5-Gluc CDS PolyA-5×UASG-Gluc-flag-BGH polyA 1..1248 bp PolyA bases 1..181 bp 5×UASG bases 182..300 bp Gluc reporter gene bases 378..932 bp Flag tag bases 939..1001 bp BGH polyA bases 1024..1248 bp