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Soy bean using iplant resources
7

Soy bean using iplant resources. How we started iPlant workshop –CSHL.

Dec 24, 2015

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Page 1: Soy bean using iplant resources. How we started iPlant workshop –CSHL.

Soy bean using iplant resources

Page 2: Soy bean using iplant resources. How we started iPlant workshop –CSHL.

How we started

• iPlant workshop –CSHL

Page 3: Soy bean using iplant resources. How we started iPlant workshop –CSHL.

Introduction

• 14 different samples – Seeds • 5, 6 and 7 stages well watered and drought stress

– Different parts of Roots• And 2 time points 72 and 96 hours as well well watered

and drought stress

Page 4: Soy bean using iplant resources. How we started iPlant workshop –CSHL.

Analysis-Quality checks

• We performed quality checks using FASTX toolkit – Locally as well as in DE

Page 5: Soy bean using iplant resources. How we started iPlant workshop –CSHL.

Analysis

• Tuxedo suite of tools• Bowtie for the alignment of the filtered data• Cufflinks suite for the analysis• We are checking the needs of the project so

that we can add more functionalities into our pipelines

Page 6: Soy bean using iplant resources. How we started iPlant workshop –CSHL.

Quality Analysis

SWS-R5 SWS-R6 SWS-R7 SWW-R5 SWW-R6 SWW-R7

Seeds

96-LRS-R2

Lateral Root

96-LRS-R1 96-LRC-R2 96-LRC-R1 72-LRS-R2 72-LRS-R1 72-LRC-R2 72-LRC-R1

Page 7: Soy bean using iplant resources. How we started iPlant workshop –CSHL.

GC contentSWS-R5 SWS-R6 SWS-R7 SWW-R5 SWW-R6 SWW-R7

Seeds

96-LRS-R2

Lateral Root

96-LRS-R1 96-LRC-R2 96-LRC-R1 72-LRS-R2 72-LRS-R1 72-LRC-R2 72-LRC-R1