Sample to Insight Single Cell Isolation Find out how you can isolate the single cell of your choice and get visual confirmation
Sample to Insight
Single Cell Isolation
Find out how you can isolate the single cell of your choice and get visual confirmation
Sample to Insight
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QIAscout: single cell isolation
High-density microraft array-based single cell isolation – simple, easy, affordable. You only require an inverted microscope to start working with the device.
Single cell genomics by QIAGEN, 2016
Sample to Insight
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Components of the QIAscout system
Understand the elegant simplicity of the QIAscout on YouTube
Single cell genomics by QIAGEN, 2016
Sample to Insight
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Discover QIAscout: single cell isolation
• Access to single cells for every lab
• Number of single cells easily scalable
• Imaging-based cell selection
• Easy to use◦ Choose the cell, release microraft to downstream reaction tube◦ Repeat as often as you want
• Affordable◦ Cost-effective device usable on multiple inverted microscopes present in labs
• Cells stay viable◦ Array can be used as a standard cell culture dish◦ Enables single cell clonal expansion
• Compatible with downstream NGS, WGA, WTA and PCR
Single cell genomics by QIAGEN, 2016
Sample to Insight
QIAscout: an easy start to your single cell analysis workflow
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Ideally suited for• Single cell isolation from cell suspensions
• Low volume, precious samples
• Adherent or non-adherent eukaryotic cells from various sample types
• Downstream molecular analyses◦ Aligned with QIAGEN’s single cell analysis portfolio
• Clonal expansion
“QIAscout turned out to be a very suitable system, allowing us to select and transfer our cell of interest with nearly 100% efficiency and survival rate for further downstream applications. The instrument has contributed greatly to our research. ”
Dr. Lajos Kemeny, University of Szeged, Hungary
Single cell genomics by QIAGEN, 2016
Sample to Insight
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The QIAscout array
• Can be used as standard cell culture dish• Microrafts are magnetic, dislodgeable
and self-sealing• Each array contains 12,000 microrafts
• Cells settle according to Poisson distribution
• 6000 cells equals a 30% chance of having a singe cell in a microraft
Single cell genomics by QIAGEN, 2016
Sample to Insight
CellTracker Green
Hoechst
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Image of cells on the QIAscout array
Sample to Insight
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After isolation cells remain viable and continue to grow
Pierced cluster of HT-29 cells
The same cluster, after several days
of cultivation
Pierced SW48 cell After severll days of cultivation
5 h 24 h 45 h 90 h
MCF-7 cell culture, established after transfer of pierced cell
HT-29 cells after 90 hours
Single cell genomics by QIAGEN, 2016
Read the application note
Sample to Insight
Single cell genomics by QIAGEN
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For in-depth, molecular analysis of single cells
Single cell WGA or WTA
Single cell isolation
Single cell sequencing
• Easily access single cells
• Affordable
• Gentle on cells
QIAscoutQIAseq
FX Single Cell Library Kits
REPLI-g Single Cell Kits
Single cell miRNA analysis
• Create superior-quality NGS libraries directly from single cells
• Provides best-in-class amplification of genomes or transcriptomes from single cells
• Profile miRNA expression using qPCR
miScript Single Cell qPCR Kit
Single cell genomics by QIAGEN, 2016
Visit the single cell resource center