Simple, Rapid Isolation of DNA from Tough-To-Lyse Fungi & Bacteria Kit Input Sample wet weight Bacterial cells Yeast cells Soft Tissue ZR Fungal/Bacterial DNA MicroPrep™ 10 - 20 mg 2 x 10 8 2 x 10 7 40 mg ZR Fungal/Bacterial DNA MiniPrep™ 50 - 100 mg 10 9 10 8 200 mg ZR Fungal/Bacterial DNA MidiPrep™ 250 - 500 mg 5 x 10 9 5 x 10 8 500 mg ZR-96 Fungal/Bacterial DNA Kit™ 10 - 20 mg/well 2 x 10 8 /well 2 x 10 7 /well 40 mg/well ZR Fungal/Bacterial DNA Kits P Isolate PCR-quality DNA from fungi and bacteria P Efficient processing with ultra- high density BashingBeads™ P Does not require the use of organic denaturants or lytic enzymes (e.g., Zymolyase) Toll-Free Main Fax Email Web 1-888-882-9682 1-714-288-9682 1-714-288-9643 [email protected] www.zymoresearch.com The ZR Fungal/Bacterial DNA MicroPrep™, ZR Fungal/Bacterial DNA MiniPrep™, ZR Fungal/ Bacterial DNA MidiPrep™, and ZR-96 Fungal/ Bacterial DNA Kit™ are designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedures are easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads™. Fast-Spin column or plate technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc. Our state of the art BashingBeads™ are constructed of the highest quality, most dense ceramic material available today. They are used when thorough sample homogenization/lysis is required by the researcher. DNA shearing by physical and chemical methods is minimized since the beads are fracture resistant and chemically inert. They are unique amongst the lysis matrices offered by other companies for DNA isolation from tough-to-lyse materials. DNA isolated from S. cerevisiae (spores) and E. coli using the ZR Fungal/ Bacterial DNA MiniPrep™ is high quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the ZR Fungal/Bacterial DNA MiniPrep™ or the kit from Supplier M. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research). Yeast Spores E. coli Zymo Research E. coli Supplier M Yeast Spores 1 kb Specifications Sample Sources Gram (+) bacteria, Gram (-) bacteria, Yeast, Filamentous fungi, Unicellular algae, Filamentous algae, Protists DNA Binding Capacities ZR Fungal/Bacterial DNA MicroPrep™: 5 µg/prep. ZR Fungal/Bacterial DNA MiniPrep™: 25 µg/prep. ZR Fungal/Bacterial DNA MidiPrep™: 125 µg/prep. ZR-96 Fungal/Bacterial DNA Kit™: 5 µg/well. Processing Times ZR Fungal/Bacterial DNA MicroPrep™: 10 min. ZR Fungal/Bacterial DNA MiniPrep™: 10 min. ZR Fungal/Bacterial DNA MidiPrep™: 20 min. ZR-96 Fungal/Bacterial DNA Kit™: 40 min. DNA Purity A 260 /A 280 > 1.8