biosecurity built on science Plant Biosecurity Cooperative Research Centre PBCRC 2061 On-farm DNA surveillance for phylloxera – an end-user perspective Inca Pearce Vinehealth Australia Suzanne McLoughlin Vinehealth Australia Andy Clarke Yering Station
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
biosecurity built on sciencePlant Biosecurity Cooperative Research Centre
PBCRC 2061
On-farm DNA surveillance for phylloxera – an end-user perspective
Inca PearceVinehealth Australia
Suzanne McLoughlinVinehealth Australia
Andy ClarkeYering Station
biosecurity built on science
A sensitive, accurate, cost-effective method for phylloxera detection for quantifying area freedom status
• “Where it is, where it is not”
Project aim
Department of Economic Development, Jobs, Transport and Resources
Exports738 million litres119 countries$2.1 billion
biosecurity built on science
Over 5,000 grape growers 135,000 hectares1.7 million tonnes
biosecurity built on science
173,000 peopleregional areas
communities, suppliers, tourism
biosecurity built on science
$40.2 billion gross output
biosecurity built on science
The problem
biosecurity built on scienceThe impact
biosecurity built on science
Well over half of the 135,000 hectares on own roots
biosecurity built on science
Yering Station own vineyards – Yarra Valley
biosecurity built on science
Maroondah Phylloxera Infested Zone
2006
biosecurity built on science
Maroondah Phylloxera Infested Zone
2009 early
biosecurity built on science
Maroondah Phylloxera Infested Zone
2009 late
biosecurity built on science
Maroondah Phylloxera Infested Zone
2013
biosecurity built on science
Maroondah Phylloxera Infested Zone
2015
biosecurity built on science
Maroondah Phylloxera Infested Zone
2016
biosecurity built on science
Maroondah Phylloxera Infested Zone
Current
biosecurity built on scienceImage courtesy of wine yarra valley
biosecurity built on science
A draft method has been developed with the following key parameters
• Field sample timing:- Detection throughout the year → highest density in summer
• Field sampling for 1 hectare block:- Every 3rd row, every 5th panel- 40 cores
• Field sample collection:- Small dig stick- 5cm from vine trunk- 0-10cm depth of soil
• Field sample handling:- Composite- Cool- To lab for process to dry soil within 48 hours
• Laboratory analysis:- qPCR technique validated with sensitivity of 1-2 phylloxera per 200g soil
Following 3 years of research…
biosecurity built on science
How does the DNA tool compare to other primary surveillance methods?
Preliminary survey of 24 vineyards identified 10 suitable on which to evaluate:• Sensitivity (proportion of positives correctly identified)• False positives • Cost • Time • Practicality
Comparative trial
DIGEMERGENCE
TRAPS DNA
biosecurity built on science
Row 1
Row 4
Row 7
Row 10
Panel 2 Panel 7 Panel 13
31 23 4
1 23 4
1 23 4
1 23 4
1 23 4
1 23 4
1 23 4
1 23 4
1 23 4
1 23 4
1 23 4
1 23 4
3
3
3
3
3
3
3 1
2
5 vineyards only
4
3W1
2
W12 Dig
DNA
DNA double sampling rate
Emergence traps
W Weak vines
biosecurity built on science
Performance of methods in detecting phylloxera
19 7 11 3
8 17 6 2
9 4
8 1
DNA and emergence trap equally as sensitive in detecting insects
and more sensitive than dig
biosecurity built on science
Phylloxera DNA by qPCR
Laboratory
Soil/root sample with corer
Identify using microscope
Laboratory
Contents of trap in ethanol
Identify using hand lens
In field
An integrated approach to phylloxera detection and surveillance
One method currently endorsed Use same sampling strategy in vineyard Important to overlay secondary methods of surveillance
DIGEMERGENCE
TRAPS DNA
Enhancing the potential of detecting phylloxera
biosecurity built on science
Next steps for our researchProtocol development
(Nov ’16)
Securing diagnostic capability (May ‘17)
National endorsement (Apr ‘17)
Case study – surveillance at >block level (Mar’17)
Delivery model to end-users (May ‘17)
Extension to and adoption by end-users (Mar ‘18)
Integration into national and state protocols/regulations
(Sep ‘17)
1
2
3
45
6
7
biosecurity built on science
REGULATORS• Early detection and ongoing surveillance• Establishment and maintenance of phylloxera zones• Enable better definition of areas of freedom (up and down)
GROWERS• Early detection, management and farm-gate hygiene• Potential for zoning change• Management of other pests & disease
INDUSTRY STAKEHOLDERS• Better defined zones will facilitate operations• Assist in quarantine process• ‘Clean, green, premium’ image maintained
RESEARCHERS• Provides a tool for studying the biology of phylloxera, rate of movement and vectors• Other soil borne pathogens and soil health
Project team- Ray Correll (Rho Environmetrics)- Alan McKay (SARDI)- Kathy Ophel-Keller (SARDI)- Cassandra Collins (The University of Adelaide)- Danièle Giblot-Ducray (SARDI)- Greg King (DEDJTR-Vic)- Chris Anderson (DPI-NSW)- Inca Pearce (Vinehealth Australia)- Suzanne McLoughlin (Vinehealth Australia)