Serial Dilution Introduction: The Purpose of serial dilution is to determine the number of bacteria per unit volume in the origi- nal culture, determination of the culture density in cells per ml. Once the culture has been diluted it can be spread on agar plates. Agar plates allow for individual bacterial cells to be separated spatially. If done correctly , there is a low probability of having two cells very close to each other. When each of these spatially separated cells multiplies, spatially separated colonies are formed. We call these clonal colonies as each visible colony arose from a single progenitor cell. The number of colonies observed is thus a direct measure of the number of bacteria spread on the surface of the plate. Serial Dilution: Gathering the tools 1. Stock Culture 2. Dilution tubes or jars (make sure to label them) 3. Pipetter 4. Agar Plates 5. Spreader
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