Sequence Analysis with Artemis & Artemis Comparison Tool (ACT) South East Asian Training Course on Bioinformatics Applied to Tropical Diseases - 2005 (Sponsored by UNDP/World Bank/WHO/TDR) International Centre For Genetic Engineering And Biotechnology , New Delhi, INDIA
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Sequence Analysis with Artemis & Artemis Comparison Tool (ACT) South East Asian Training Course on Bioinformatics Applied to Tropical Diseases - 2005 (Sponsored.
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Sequence Analysis with Artemis &
Artemis Comparison Tool (ACT)South East Asian Training Course on
Bioinformatics Applied to Tropical Diseases - 2005
(Sponsored by UNDP/World Bank/WHO/TDR)
International Centre For Genetic Engineering And Biotechnology ,New Delhi, INDIA
Overview of the genome sequencing and sequence analysis.
Demonstration of Artemis.
Hands on guided exercise in Artemis.
Demonstration of ACT .
Hands on guided exercise in ACT
Generating ACT comparison files
Workshop Overview
Wellcome Trust Photo Library
The Wellcome Trust Sanger Institute
•Funded by The Wellcome Trust, a registered charity.•Established in 1993 to begin the Human genome project. •First Draft (2000) complete (2003-4)
Data release policy:
All sequence data is released immediately and is freely available via the internet in order to maximise its benefit for research.
http://www.sanger.ac.ukftp://ftp.sanger.ac.uk/
Wellcome Trust Photo Library
Generating the complete genome sequence
Infrastructure
Levels of automation
Colony pickingrobots
Plasmid prepsrobots
TOTAL:140
ABI3700
ABI3730
Automated sequencing
Each ABI reads 96 DNAsequences at once.
The machines are run10 times a day,7 days a week.
Throughput of 1,200 to 1,300 96-well plates per day ± 120,000 DNA samples read each day.
Each day, the Sanger Institute reads 60 million base pairs. That’sequal to one of the smaller human chromosomes and many timesthat of an average bacterial genome.