University of Coimbra Universidade de Coimbra Separation and characterization of extracted bioactive phytochemicals = Production of phytochemicals through extraction with supercritical fluids Mara Elga Medeiros Braga GSP - Green and Sustainable Processes Lab CIEPQPF, Chemical Engineering Department, University of Coimbra, Coimbra, Portugal
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Separation and characterization of extracted bioactive ... · extracted bioactive phytochemicals = Production of phytochemicals through ... is for medicinal plants to be used not
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Braga et al, Journal of Supercritical Fluid, 34, 149-156, 2005.
Lippia alba leaves
Solubility of compounds: carvone and limonene
University of Coimbra
Universidade de Coimbra
Braga et al, Journal of Supercritical Fluid, 34, 149-156, 2005.
Lippia alba leaves
Solubility of compounds: carvone and limonene
University of Coimbra
Universidade de Coimbra
Curcuma longa roots
Cosolvent
Braga et al. Journal of Agricultural and Food Chemistry. 51, 6604-6611, 2003.
University of Coimbra
Universidade de Coimbra
Braga, M. E. M.; Meireles, M. A. A. Journal of Food Process Engineering, 30, 501-521, 2007.
HB/DB rates
Fractionation and cosolvent
Static period
Curcuma longa roots
University of Coimbra
Universidade de Coimbra
Seabra et al. Fractioned High Pressure Extraction of
Anthocyanins from Elderberry (Sambucus nigra L.) Pomace.
Sambucus nigra pomace
Raw material
Extraction method: SFE, ESE and CSE
University of Coimbra
Universidade de Coimbra
University of Coimbra
Universidade de Coimbra
Caesalpinea spinosa – seed coats
Representation of the assayed solvent mixtures with
diverse CO2, EtOH and H2O molar fractions
Ternary solvent composition
Durling et al. Fluid Phase Equilibria 252 (2007) 103–113
Seabra et al, J. of Supercritical Fluids 64 (2012) 9– 18
yield phenols
Antioxidant
activity
Anti-inflammatory
activity
University of Coimbra
Universidade de Coimbra
Dias et al. Jambu (Spilanthes oleraceae) global extraction yields obtained from flowers, leaves and stems
when using different extraction methods and solvent mixtures. J.Supercritical Fluids, 61 (2012) 62– 70
Spilanthes oleraceae Solvent composition
Parts of plant
University of Coimbra
Universidade de Coimbra
Dias et al. Jambu (Spilanthes oleraceae) global extraction yields obtained from flowers, leaves and stems
when using different extraction methods and solvent mixtures. J.Supercritical Fluids, 61 (2012) 62– 70
Spilanthes oleraceae Solvent composition
Parts of plant
University of Coimbra
Universidade de Coimbra
Dias et al. Jambu (Spilanthes oleraceae) global extraction yields obtained from flowers, leaves and stems
when using different extraction methods and solvent mixtures. J.Supercritical Fluids, 61 (2012) 62– 70
Spilanthes oleraceae Solvent composition
Parts of plant
University of Coimbra
Universidade de Coimbra
Anti-inflammatory activities synergism among compounds
Dias et al. Jambu (Spilanthes oleraceae) global extraction yields obtained from flowers, leaves and stems
when using different extraction methods and solvent mixtures. J.Supercritical Fluids, 61 (2012) 62– 70
University of Coimbra
Universidade de Coimbra
Talansier et al. J. of Supercritical Fluids 47, 200–208, 2008.
Vetiveria zizanioides roots Pressure and temperature
Cosolvent
Kinetic study
University of Coimbra
Universidade de Coimbra
Braga et al. Journal of Supercritical Fluids, 47, 37-48, 2008.
Pinus Pinaster bark Fractionation and flow rate
Fig. 2. Pine bark FSFE kinetics results. Experiments at 40 ◦C and at ∼20MPa:
(□) 1st step CO2 extraction and (■) 2nd step CO2 + EtOH (10%) extraction.
University of Coimbra
Universidade de Coimbra
Braga et al. Journal of Supercritical Fluids, 47, 37-48, 2008.
Pinus Pinaster bark Fractionation and flow rate
solvent flow rate: low medium high
Seabra et al. Journal of Supercritical Fluids, 62 (2012) 135– 148.
University of Coimbra
Universidade de Coimbra
Serra et al. J. of Supercritical Fluids 54, 2010.
Prunus avium fruits
Flow rate and Fractionation
University of Coimbra
Universidade de Coimbra
Serra et al. J. of Supercritical Fluids 54, 2010.
Prunus avium fruits
Flow rate and Fractionation
Antiproliferative activity of cherry extracts in human colon cancer cells.
Category: Products’ development and innovation
University of Coimbra
Universidade de Coimbra
Impregnation of phytochemicals
into polymeric matrices for
biomedical applications
University of Coimbra
Universidade de Coimbra
Supercritical Fluid Impregnation
(SSI)
Bioactive Wound Dressing
Biopolymers
foams/films
Quercetin/Thymol
Biomolecules
“Tunable” loaded
materials
Quercetin
Thymol
Supercritical Fluid Impregnation
(SSI)
Bioactive Wound Dressing
Biopolymers
foams/films
Quercetin/Thymol
Biomolecules
“Tunable” loaded
materials
Supercritical Fluid Impregnation
(SSI)
Bioactive Wound Dressing
Biopolymers
foams/films
Quercetin/Thymol
Biomolecules
“Tunable” loaded
materials
Supercritical Fluid Impregnation
(SSI)
Bioactive Wound Dressing
Biopolymers
foams/films
Quercetin/Thymol
Biomolecules
“Tunable” loaded
materials
Supercritical Fluid Impregnation
(SSI)
Bioactive Wound Dressing
Biopolymers
foams/films
Quercetin/Thymol
Biomolecules
“Tunable” loaded
materials
Thymol
Quercetin
Dias et al, Development of Natural-based Wound Dressings Impregnated with Bioactive Compounds Using Supercritical Carbon Dioxide, International Journal Pharmaceutics, 408 (2011) 9-19.
University of Coimbra
Universidade de Coimbra
Non-loaded
dressings
Jucá - loaded
dressings
Bioactive Wound Dressing: comparison among commercial membranes and CBC
Dias, et al. Wound dressings loaded with an anti-inflammatory jucá (Libidibia ferrea) extract using supercritical carbon dioxide technology. The Journal of Supercritical Fluids, 74 (2013) 34– 45.
University of Coimbra
Universidade de Coimbra
Conclusions
SCF’s have real advantages and can be successfully used for
development of several industrial applications, namely of pharmaceutical
and food applications and when classical methods are no more
employable or have important disadvantages.
Different SCF technological processes can be used and the choice largely
depends on raw materials to be treated and on the final end-use required;
SCF’s may be an excellent alternative proposal to traditional solvent
extraction methods;
Most of SCF techniques (including SFE) are solvent-free and leave dry,
dispersed end-products;
University of Coimbra
Universidade de Coimbra
Most of SCF’s of interest have low critical temperatures (like CO2), thus
do not degrade thermo-labile and bio-labile substances;
Economic evaluation of SCF processes shows that, for high added-value
products like nutraceuticals and pharmaceuticals, they can be profitable,
in spite of their higher initial investments and higher operational costs;
However, they are clearly “environmentally” profitable: SC Processes are
often referred as “Green”, “Clean”, “Sustainable Technology” and
“Environmental Friendly” processes;
Other SCF processes can be combined: SSI, particle and emulsion
production, chemical modifications in SCF, scCO2 sterilization, protein
precipitation, fractionation and purification, SCF chromatography…
University of Coimbra
Universidade de Coimbra
Collaborations team:
Portugal :
Faculdade de Farmácia – Universidade de Coimbra
Instituto de Tecnologia Química e Biológica – ITQB, UNL
Escola Superior Agrária de Coimbra -ESAC
Cooperativa do Vale de Varosa
Curtumes J.B. Salgueiro
Brazil, Spain and Colombia:
Universidade de Sao Paulo - USP
Universidade Federal do Pará – UFPA
Universidade Federal da Bahia – UFBA
Embrapa Agroindústria Tropical
Universidad de Santiago de Compostela, Facultad de Farmacia
Universidad Nacional de Colombia, Departamento de Farmacia