Selected and Differential Media. Define Selective Media Differential Media Enriched Media.

Selected and Differential Media

Define

Selective Media Differential Media Enriched Media

Blood Agar Plate (BAP)

Differential Enriched Use

Growth Hemolysis

Alpha Beta Gamma

C-CNA

Selective Differential Results

Gram (+) Colistin Naldixic acid

Blood Hemolysis

MSA

Selective Differential Results

Halotolerant (7.5% NaCl) CH2O: Mannitol pH

phenol red Fermentation

Acid Yellow

MAC

Selective Differential Results

Gram (-) CV Bile Salts

Lactose pH indicator

Neutral red indicator Ferment = pink/red No acid = colorless

EMB

Selective Differential Results

Gram (-) Eosin dye MB dye

Lactose Some acid = pink Large acid = green No acid = colorless

Set Up

One plate for each group Label quadrants as lab

book indicates for each plate

Cultures on cart Anaerobic Gas Pack

BAP C-CNA

All others aerobic 37°C

BAP results

Sheep blood enriched Growth most all microbes Fastidious Differential

GP Hemolysins Hemolysis types

Gamma Alpha Beta

Hemolysis

No hemolysis

C-CNA results

Selective Growth GP

Colistin Nalidixic acid

Differential Hemolysis

MSA results

Selective 7.5% NaCl Staphylococcus spDifferential Mannitol fermentation phenol red yellow

MAC results

Selective: GN CV Bile Salts

Differential Lactose fermentation NF = colorless Coliforms

Neutral red pink

EMB results

Selective: GN Dyes

Eosin MB

Differential Lactose fermentation NF = colorless Acid production

Small = pink Large = metallic green