SCIENTIFIC RESULTS OF TRANS2CARE PROJECT - Lovro Ziberna

SCIENTIFIC RESULTS OF

TRANS2CARE PROJECT

Lovro Žiberna, PhD MPharm

LP – University of Trieste

Trieste, September 11, 2014

Final conference

1. cell biology, membrane transport

2. statistics, chemometrics

3. transcriptome analysis

4. analytical chemistry

5. genetics, clinical markers

6. IP management

7. clinical chemistry in intensive care

8. electrochemistry

9. anti-microbial peptides

10. IHC, clinical specimens

11. antibody production, stem cells

12. biocompatible materials

13. genetics, clinical markers

THE SCIENCE OF OUR Partners

can we link

local

Institutions by

research

synergies?

CLINICIANSBASIC

SCIENTISTSTECHNO-LOGISTS

Unmet medical needsScientific understanding and possible solutions Technical solutions

TRANS2CARE at start

1. CARDIOVASCULAR DISEASES2. NEURODEGENERATIVE

DISEASES3. CANCER4. OBESITY5. ORTHOPAEDIC DISEASES6. AUTOIMMUNE DISEASES

1. Nephrotoxicity following coronarystent

2. Prion diseases3. Nutrigenomics of obesity4. R&D on implants lifespan5. Bio-availability and bioactivity of

dietary polyphenols6. Coeliac disease

1. Computational and statistical support

2. Analytical chemistry3. Research antibodies4. Nanoparticles, biosensors5. Tech transfer, IP protection

PP2

priondiseases

PP10

diagnosticmAbs

PP6nefrotoxicity

post-coronarydilation

PP6, PP12

plasma free bilirubin

LP

membrane transport

PP9anti-

transglutaminaseantibodies in plasma

PP4

Klothoprotein in

plasma PP7Electro-chemical

biosensors

PP3

Analyticalchemistry

br

NGAL

cytotox

br

Ab

Klo

Ab-BTL

Ab-prion

PP1chemometrics

BTL

prion

PP5training

tech transferIP

PP8

BTL in plantanti-microb.

peptides PP11

biomaterialsfor implants

AMP

Ab-BTL

IDEAS PRODUCTS

technical tools

pre-clinical studies

clinical applications

0

2

4

6

8

10

12

14

LP PP1 PP2 PP3 PP4 PP5 PP6 PP7 PP8 PP9 PP10 PP11 PP12

nu

mb

er

of

pu

blic

atio

ns

scientific papers/bookchapters

active contributions atthe scientificconference

papers in preparation

SCIENTIFIC OUTPUT OF T2C RESEARCHERS

2.38 scientific papers/researcher

3.77 active conference contributions/ researcher

>1 T2C partner (networking):

20.7 % of all scientific papers

37.8 % of all conference contributions

0

5

10

15

20

25

30

scientific papers/book chapters

active contributions at the scientificconference

1

>1

620

23

29

SCIENTIFIC OUTPUT OF T2C RESEARCHERS

pre-disease screening

21st century medicine

focused on identifying the window of opportunity before the full development of pathology (REVERSIBLE CHANGES)

healthy aging

disease diagnosis and causative treatment

20th century medicine

focused on diagnostics (after pathology fully developed) and treatment: pharmacology and/or surgery

chronic treatment to keep body close to homeostasis (IRREVERSIBLE CHANGES)

alleviation of symptoms

pre-historic – 19th century medicine

focused on reducing the pain or other symptoms

NO CAUSATIVE THERAPY

PALLIATIVE THERAPY

cardiovascular

diseases

genotype

diet

physical activity

mental activity

psycho-social activity

biomarkers

no clinical signsbiomarkers

clinical signs biomarkers

clinical signs

deathASYMPTOMATIC ≠ HEALTHY

overweight/ob

esity,

dyslipidemia,

mild

hypertension

acute myocardial

infarction,

arrhythmia,

hypertension

heart failure

(NYHA I-IV),

cardiac death

BIRTH DISEASE DEATH

OBESITY AND OVERWEIGHT

► how specific dietary compounds intake modulates the

gene expression of the adipokines (nutrigenomics)

► interaction between specific dietary compound

and certain genetic polymorphisms by using

Kohonen neural network (nutrigenomics and

personalized medicine) new knowledge on food

and gene interactions to design better diet protocols

ANA PETELIN

ANA PETELIN KATJA VENKO

OBESITY AND OVERWEIGHT

► Identification of early biomarkers of metabolic

imbalance in healthy subjects

► bilirubin as a pre-disease biomarker in

asymptomatic individuals

ANA PETELIN LOVRO ŽIBERNA

CANCER AND LIFESTYLES

“local adjuvant therapy” by anthocyanins-enriched diet

for colon cancer patients

focus on membrane transporters involved in uptake

(bilitranslocase) and efflux (multridrug resistance

proteins) in GIT

JOVANA ČVOROVIĆ

UROŠ RAJČEVIĆ morphological and proteomic characterization of

organotypic spheroids derived from colorectal

cancer tissue

CANCER AND LIFESTYLES

new histological biomarker (expression of

bilitranslocase) for the diagnosis of kidney

cancer

development of anti-bilitranslocase monoclonal

antibodies (diagnostic tool)

UROŠ RAJČEVIĆ LUIGINA DE LEO

NEURODEGENERATION

large-scale transcriptional profiling of brains

from prion-infected macaques, which are an

excellent model for human prion disorders

identification of potential disease biomarkers

and novel potential targets for therapeutics

MAURA BARBISIN KATJA VENKO

novel predictive computational model

(artificial neural network model) for

designing new compounds with anti-

prion activity that can be further

experimentally tested using cell-screening

assay pre-clinical studies

CARDIOVASCULAR DISEASES

interventional cardiology iodine contrast-induced

nephropathy

detection of early signs of nephropathy by

serum biomarker NGAL

POLONA LIKAR MITJA MARTELANC

POLONA LIKAR LOVRO ŽIBERNA

pharmacological and nutraceutical modulations of

contrast-induced toxicity on human vascular

endothelial cells

COELIAC DISEASE

double immunofluorescence analysis allowed the

early diagnosis in subjects at high risk of celiac

disease

pre-disease diagnosis (early diet modification leads

to better prognosis)

LUIGINA DE LEO

LUIGINA DE LEO MORENA SILVESTRINI

development of electrochemical

biosensors for the detection of different

autoantibodies (e.g., anti-tTG), anti-

gliadin, anti-thyroid peroxidase (anti-TPO)

involved in celiac disease and associated

autoimmune diseases, directly in biological

samples

new technology: electrochemical

impedance spectroscopy

BONE HEALTH

improving life-span of orthopedic implants by the

prevention of prosthetic joint infections

incorporation/immobilization of antimicrobial

peptides (AMPs) into biopolymeric matrices as

potential coatings for orthopedic implants.

FRANJA ŠULEK FRANCESCA D’ESTE

ALESSANDRO BALDAN correlation between blood biomarker Klotho

factor and bone mineral density

lower Klotho levels in peripheral blood are linked to

osteopenia and osteoporosis in the femur

DIAGNOSTIC BIOMARKERS

novel method for the detection of free bilirubin in

human serum (non-routine clinical measurement of

the bioactive form) – around 10 nM in human serum

MITJA MARTELANC

fast, cheap and easy-to use sensing

devices for the detection of molecules of

clinical importance, directly in biological

samples

improved method for total and

conjugated bilirubin detection in serum

samples using a novel biosensor based

on electrochemical detection

LOVRO ŽIBERNA

MORENA SILVESTRINI

INTELLECTUAL PROPERTY

open innovation

“Think before you publish.” “Perhaps, patent?”

creation of a necessary scientific bridge between

a number of entities:

- Universities; - Big industries;

- Research centres; - Tech transfer offices;

- The market; - Public institutions;

- SMEs; - Laboratories

• good connection improves the outputs of scientific

research

GIORGIA FAVARO

IP

BRIDGE

Academics

Big industries

ResearchCentres

TechTransfer Offices

Market

Public Insitution

s

SMEs

Laboratories

Trans2Care workpackagesEmbedding training in the network’s activities

WP6TECH

TRANSFER

WP2PREPARATORY

ACTIVITY

WP7SUSTAINABILITY

WP8DISSEMINATION

WP5MAPPING &

ROAD-MAPPING

COORDINATION

WP1MANAGEMENT

WP4part 1

TECHNOLOGICAL TRAINING

WP3RECRUITMENT

OF 14 RESEARCHERS

WP4 part 2

COMPLEMENTARYTRAINING

EVOLUTION OF HUMAN CAPITAL

The 14 researchers have done

large parts of WP 6-8

8 WPs

WP 6 as the core

WP 5,7,8ancillary to WP6

Training: WP4 and transversal to WPs

scientific research = technological training

SCIENTIFIC COMMUNICATION

OTHER SCIENTIFIC ACTIVITIESFocus on knowledge sharing and developing integrated skills

• acting as reviewers of original research manuscripts for SCI

scientific journals

• tutoring and co-mentorships of PhD and undergraduate students

• submission of post-doctoral grant applications

• active involvement in research discussion at online scientific

forums

• participation at online scientific webinars

Progetto Rete Transregionale per l’ innovazione ed il Trasferimento Tecnologico per ilMiglioramento della Sanità finanziato nell’ambito del Programma per la CooperazioneTransfrontaliera Italia-Slovenia 2007-2013, dal Fondo europeo di sviluppo regionale e dai fondinazionali.Projekt Transregionalno omrežje za inovacijo in prenos tehnološkega znanja za izboljšanjezdravstva sofinancirana v okviru Programa čezmejnega sodelovanja Slovenija-Italija 2007-2013

iz sredstev Evropskega sklada za regionalni razvoj in nacionalnih sredstev.

Thank you for the attention !

Partners: LP – Università degli Studi di Trieste, PP1 – Kemijski Inštitut Ljubljana, PP1 – Kemijski Inštitut Ljubljana, PP2 – ScuolaInternazionale Superiore di Studi Avanzati, PP3 – Univerza v Novi Gorici, PP4 – Università di Ferrara, PP5 – Treviso Tecnologia, PP6 –Splošna Bolnišnica Dr. Franca Derganca, PP7 – Università Ca’ Foscari di Venezia, PP8 – Università di Udine, PP9 – IRCCS Burlo Garofalo,PP10 – Zavod Republike Slovenije Za Transfuzijsko Medicino, PP11 – Ortopedska Bolnišnica Valdoltra, PP12 – Univerza na Primorskem -Fakulteta za Vede o Zdravju.