SCHOTT SCHOTT NEXTERION MTP-96 Maximum & Minimum filling volumes per well
Dec 18, 2015
SCHOTT
SCHOTT NEXTERION MTP-96Maximum & Minimum filling volumes per well
SCHOTT TGS PRODUCT GROUP NEXTERION
© SCHOTT Technical Glass Solutions GmbH
Corinna Schwung, 04/18/23
Experimental Details
Substrate tested:
- MTP 2-2259 without patterning 18.01.11
Buffer Solutions tested:
- PBS (1x) with 0.08 mg/ml Dye 5
- PBST 0.1% Tween with 0.08 mg/ml Dye 5
SCHOTT TGS PRODUCT GROUP NEXTERION
Corinna Schwung, 04/18/23
© SCHOTT Technical Glass Solutions GmbH
Filling Scheme
1 2 3 4 5 6 7 8 9 10 11 12
A 130 140 150 160 170 180 190 200 210 220 230 240
B 130 140 150 160 170 180 190 200 210 220 230 240
C 10 20 30 40 50 60 70 80 90 100 110 120
D 10 20 30 40 50 60 70 80 90 100 110 120
E 10 20 30 40 50 60 70 80 90 100 110 120
F 10 20 30 40 50 60 70 80 90 100 110 120
G 130 140 150 160 170 180 190 200 210 220 230 240
H 130 140 150 160 170 180 190 200 210 220 230 240
PBST
Volume in µl
PBS
These wells were filled up to maximum liquid level.
SCHOTT TGS PRODUCT GROUP NEXTERION
Corinna Schwung, 04/18/23
© SCHOTT Technical Glass Solutions GmbH
Results from adding 10 – 120 µl per well
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
- Solution pipetted into upper left corner
- MTP was gently shaken
Minimum Volumes
- PBST: 40 µl
- PBS: 50 µl
PBST
PBS
10 µl 120 µl
SCHOTT TGS PRODUCT GROUP NEXTERION
Corinna Schwung, 04/18/23
© SCHOTT Technical Glass Solutions GmbH
Results from adding 10 – 240 µl per well
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
PBST
PBS
10 µl 120 µl
130 µl 240 µl
PBST
PBS
130 µl 240 µl
SCHOTT TGS PRODUCT GROUP NEXTERION
Corinna Schwung, 04/18/23
© SCHOTT Technical Glass Solutions GmbH
Results from adding 10 – 240 µl per well13
0 µl
240
µl
PBS
PBST14
0 µl
150
µl
160
µl
170
µl
180
µl
190
µl
200
µl
210
µl
220
µl
230
µl
PBS
PBST
130
µl
240
µl
140
µl
150
µl
160
µl
170
µl
180
µl
190
µl
200
µl
210
µl
220
µl
230
µl
- Safe filling volume is about 160 µl for PBS, 130 µl for PBST
SCHOTT TGS PRODUCT GROUP NEXTERION
Corinna Schwung, 04/18/23
© SCHOTT Technical Glass Solutions GmbH
Results from adding 10 – 240 µl per well
PBS
PBST
- The maximum filling level was not reached with these volumes
240
µl
190
µl
200
µl
210
µl
220
µl
230
µl
240
µl
190
µl
200
µl
210
µl
220
µl
230
µl
SCHOTT TGS PRODUCT GROUP NEXTERION
Corinna Schwung, 04/18/23
© SCHOTT Technical Glass Solutions GmbH
Results from adding 320 – 400 µl per well
PBS
PBST
400
µl
370
µl
380
µl
390
µl
320
µl
350
µl
340
µl
330
µl
Maximum Volume per well
- PBS: 410 µl
- PBST: 340 µl
SCHOTT TGS PRODUCT GROUP NEXTERION
Corinna Schwung, 04/18/23
© SCHOTT Technical Glass Solutions GmbH
Conclusions
The minimum volume that will cover the well bottom is: 40 microlitres (with detergent) 50 microlitres (without detergent)
The maximum recommended „safe“ working volume is: 130 microlitres (with detergent) 160 microlitres (without detergent)
SCHOTT
SCHOTT NEXTERION MTP-96Maximum & Minimum filling volumes per well