De La Salle University Science Foundation, Inc. SCHEDULE OF SCIENTIFIC RESEARCH & DEVELOPMENT EXPENSES For the Period June 1, 2014 to May 31, 2015 Ongoing Projects Assessment of Airborne Bacterial Flora in the Intensive Care Unit, Operation Room, Emergency Room and Nursery of the Ospital ng Muntinlupa: A Multidisciplinary Approach 03 IR U/S 1TAY12-1TAY13 Dr. Esperanza Cabrera, Dr. Edgar Vallar, and Dr. Oscar Torres Project Direct Expense: Php 150,580.00 Abstract: Bacterial pathogens are the most recognized nosocomial-associated (healthcare-associated infections, HAI) pathogens (Singh et al., 2006). Airborne bacterial transmission occurs through aerosol droplets from coughing and sneezing, which can remain suspended for some time in the air and can travel over considerable distances. The predisposition of patients to HAI depends on the nature of their diseases and their management (Mayon-White et al., 1988). The proposed study aims to determine the microbial load and identities of the bacteria that might be present in the intensive care unit, operating room, emergency room, and nursery of the Ospital ng Muntinlupa using phenotypic and genotypic methods along with proteomics and fluoroscopic studies. Figure 1. The tertiary government hospital in Alabang, Muntinlupa where air sampling was conducted Air Sampler Figure 2. Operating room unit where air sampling was conducted. The air sampler was placed at the same height as the operating table Figure 3. Nursery room. The air sampler was placed on a table that was of the same height as the cribs for the babies.
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SCHEDULE OF SCIENTIFIC RESEARCH ......Analysis of Omega-3-Fatty Acids and Heavy Metals in Eucheuma denticulatum (Guso) and Caulerpa lentillifera (Lato) 32 F U/S 1TAY14-3TAY14 Dr. Nancy
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De La Salle University Science Foundation, Inc.
SCHEDULE OF SCIENTIFIC RESEARCH & DEVELOPMENT EXPENSES
For the Period June 1, 2014 to May 31, 2015
Ongoing Projects Assessment of Airborne Bacterial Flora in the Intensive Care Unit, Operation Room, Emergency Room and
Nursery of the Ospital ng Muntinlupa: A Multidisciplinary Approach
03 IR U/S 1TAY12-1TAY13
Dr. Esperanza Cabrera, Dr. Edgar Vallar, and Dr. Oscar Torres
Project Direct Expense: Php 150,580.00
Abstract: Bacterial pathogens are the most recognized nosocomial-associated (healthcare-associated
infections, HAI) pathogens (Singh et al., 2006). Airborne bacterial transmission occurs through aerosol
droplets from coughing and sneezing, which can remain suspended for some time in the air and can travel
over considerable distances. The predisposition of patients to HAI depends on the nature of their diseases
and their management (Mayon-White et al., 1988). The proposed study aims to determine the microbial
load and identities of the bacteria that might be present in the intensive care unit, operating room,
emergency room, and nursery of the Ospital ng Muntinlupa using phenotypic and genotypic methods along
with proteomics and fluoroscopic studies.
Figure 1. The tertiary government hospital in Alabang,
Muntinlupa where air sampling was conducted
Air Sampler
Figure 2. Operating room unit where air sampling was conducted.
The air sampler was placed at the same height as the operating table
Figure 3. Nursery room. The air sampler was placed on
a table that was of the same height as the cribs for the
babies.
Assisted Direct 2'-Fluorination of Nucleosides
14 F U S 1TAY13-1TAY14
Dr. Gladys Cherisse Completo
Project Direct Expense: Php 297,041.90
Abstract: 2’-(Fluoroarabinofuranosyl)uridine was synthesized using a series of protection and deprotection
at 2’-, 3’-, and 5’- hydroxyl groups of ribose moiety and N-3 position of pyrimidine group of a preformed
nucleoside uridine. A cyclic protecting group was introduced at the 3'- and 5'-position of the ribose sugar to
lock the ring in a rigid conformation where the uridine base is not sterically hindering and to permit the
topside attack of fluoride. An electron withdrawing protecting group was placed at 2’-OH position followed
by the protection at N-3 position. Late stage fluorination using KF is employed via SN2 mechanism.
Analysis of Omega-3-Fatty Acids and Heavy Metals in
Eucheuma denticulatum (Guso) and Caulerpa lentillifera (Lato)
32 F U/S 1TAY14-3TAY14
Dr. Nancy Llanos
Project Direct Expense: Php 82,501.75
Abstract: Popular seaweeds used in salads and other delicacies Eucheuma denticulatum (Guso) and
Caulerpa lentillifera (Lato) will be analyzed for their omega-3 fatty acid content as well as heavy metals.
Samples will be collected from wet markets in selected locations. The lipid will be extracted according to a
modified version by Folch and the fatty acids will be analyzed using gas chromatography as fatty acid
methyl esters using known standards. The amount of selected heavy metals will be analyzed using atomic
absorption spectroscopy. Data will be treated using applicable statistical tool.
This study will guide consumers on the possible benefits and risks of seaweed consumption. Also, the data
generated in this project can be used for policy makers for the protection of our marine resources.
Furthermore, it can be used by appropriate government agencies who are tasked to ensure that food sold in
the market is safe for consumption.
Morphological and Thickness Analyses of Spin-Coated Nafion Films
13 IR U/S 3TAY12-3TAY13
Dr. Shirley Tiong-Palisoc, Dr. Michelle Natividad, and Mr. Frumencio Co
Project Direct Expense: Php 228,981.08
PUBLISHED PAPER:
Shirley Palisoc, Michelle Natividad, Frumencio Co, Kevin Kaw
Morphological, thickness and electrochemical analyses of spin-coated [Ru(NH3)6]3+/Nafion films
Abstract: Nafion thin films were fabricated using the spin coating method. Hexaammineruthenium(III)
[Ru(NH3)6]3+ was used as the redox mediator and was incorporated in the film. The amount of
[Ru(NH3)6]3+ was varied and three different solvents were used. The morphology of the films was
investigated and the film thickness was measured using scanning electron microscopy (SEM). The effects
of varying the amount of the redox mediator and the solvent type on the viscosity of the coating solution,
film thickness and morphology were investigated. The electrochemical properties of the fabricated films
were studied using cyclic voltammetry.
Fig. 1. Viscosity of the coating solutions.
Fig. 2 SEM images showing the thickness of the
films with (a) 10wt% YSZ, (b) 30wt% YSZ, (c)
50wt% YSZ fabricated on Silica.
Theoretical Studies on Quantum Transport Properties of Boron Nitride Nanotubes
04 IR U/S 1TAY12-1TAY13
Dr. Melanie David, Dr. Nelson Arboleda, Dr. Emmanuel Rodulfo, Dr. Al Rey Villagracia, and
Mr. John Vincent Morales
Project Direct Expense: Php 811.00
Abstract: The team reports the effects of defects on the quantum transport properties of boron nitride
nanotubes. The formation energies and band gap of 115 BNNT systems were calculated using density
functional theory corresponding to its pristine form, single boron/nitrogen vacancies and carbon
substitution, and two atom vacancies and substitutions at defect/doping concentrations from 0.83% to
1.67%. Results showed that single nitrogen vacancy, single boron vacancy, single carbon-nitrogen
substitution, and single carbon-boron substitution are the most favorable systems based on formation
energy following that order among the defects. These favorable systems have a band gap ranging from
0.493 eV to 3.777 eV which were significantly smaller than the pristine BNNT’s band gap of 4.268 eV.
The current-voltage (I-V) characteristics of pristine and the four favorable vacancy and doping systems
were calculated using Keldysh formalism or non-equilibrium Green’s function formalism (NEGF). Results
demonstrated a diode-like I-V characteristics which is in good agreement with experimental results.
Figure 1. Pristine Boron Nitride Nanotube (BNNT)
(a) (b) Figure 2. Single (a) carbon-boron/nitrogen substitution and (b) boron vacancy. The circles indicate the locations for substitution and vacancy.
Figure 3. The BNNT structure for B-vacancy and carbon substitution. A, B, C and D are for the different rings, the number 1
at the right is for the reference atom, and the rest are next vacancy or substitution. For example, the yellow mark at the left figure
corresponds to 1A.
Figure 4. The BNNT structure for N vacancy and carbon substitution. A, B, C and D are for the different rings, the number 1 at the right is for the reference atom, and the rest are next vacancy or substitution. For example, the yellow mark at the left
figure corresponds to 1A.
Figure 5. The BNNT structure for NB vacancy and carbon substitution. A, B, C, D and Z are for the different rings, the yellow mark (nitrogen) at the left is for the reference atom, and the numbered atoms are for vacancy or substitution. For example,
the yellow mark (nitrogen) of ring A at the left figure paired with number 1 boron corresponds to 1A.
Left semi-infinite electrode Central scattering region Right semi-infinite electrode
Figure 6. Schematic illustration of a two-probe system for a pristine BNNT
Isolation, Structure Elucidation and Cytotoxicity Studies on Six Plants from DLSU-STC
04 IR U/S 1TAY14-3TAY14
Dr. Mariquit Delos Reyes, Dr. Consolacion Ragasa, and Mr. Glenn Oyong
Project Direct Expense: Php 174,195.00 PUBLISHED PAPERS:
Consolacion Y. Ragasa, Virgilio Ebajo Jr., Mariquit M. De Los Reyes, Emelina H. Mandia, Robert
Brkljača and Sylvia
Triterpenes from Calophyllum inophyllum Linn. International Journal of Pharmacognosy and
Phytochemical Research 2015; 7(4); Galley Proof ISSN: 0975-4873
Abstract: Chemical investigation of the dichloromethane extracts of Calophyllum inophyllum Linn.
afforded friedelin (1), squalene (2), canophyllic acid (3), and a mixture of canophyllalic acid (4a) and
canophyllol (4b) from the leaves; and 1 and taraxasterol (5) from the twigs. The structures of 3-5 were
elucidated by extensive 1D and 2D NMR spectroscopy, while those of 1 and 2 were identified by
comparison of their NMR data with those reported in the literature.
Consolacion Y. Ragasa, Vincent Antonio S. Ng , Mariquit M. De Los Reyes, Emelina H. Mandia , Glenn
G. Oyong and Chien-Chang Shen
Chemical constituents and cytotoxicity of the leaves of Dysoxylum gaudichaudianum (A. Juss.) Miq. Der
Pharma Chemica, 6(5), 2014
Abstract: The crude dicloromethane leaf extract of Dysoxylum gaudichaudianum (A. Juss.) Miq. was tested
for cytotoxicity against breast cancer (MCF-7) and colon cancer (HT-29) cells and exhibited IC50 values of
7.35 and 13.19 µg/mL, respectively. Chemical investigation of the dichloromethane leaf extract afforded
squalene (1), β-sitosterol (2), polyprenols (3) and triglyceride (4). The structures of 1-4 were identified by
comparison of their 1H NMR data with those reported in the literature. These compounds are known
cytotoxic and anticancer compounds.
Consolacion Y. Ragasa, Vincent Antonio S. Ng, Mariquit M. Delos Reyes, Emelina H. Mandia and Chien-
Chang Shen
Triterpenes and a coumarin derivative from Kibatalia gitingensis (Elm.) Woodson. Der Pharma Chemica,
2014, 6(5):360-364
Abstract: Chemical investigations of the dichloromethane extracts of Kibatalia gitingensis (Elm.)
Woodson afforded ursolic acid (1), squalene (2), α-amyrin acetate (3) and lupeol acetate (4) from the
leaves, while the twigs yielded 1-4 and 6-hydroxy-7-methoxycoumarin or isoscopoletin (5). The structures
of 1 and 5 were elucidated by extensive 1D and 2D NMR spectroscopy, while 2-4 were identified by
comparison of their 13C NMR data with those reported in the literature.
Mariquit M. De Los Reyes, Glenn G. Oyong, Virgilio D. Ebajo Jr., Vincent Antonio S. Ng, Chien-Chang
Shen, Consolacion Y. Ragasa
Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob. Journal of Applied
Pharmaceutical Science Vol. 5 (11), pp. 023-030, November, 2015
Abstract: The triterpenes, squalene (1), friedelin (2) and a mixture of ursolic acid (3a) and oleanolic acid
(3b) in a 2:3 ratio, and a mixture of β-sitosterol (4a) and stigmasterol (4b) in a 2:1 ratio, obtained from the
dichloromethane extract of Pipturus arborescens (Link) C.B. Rob., were evaluated for their anti-
proliferative activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-
116), and a normal cell line, human dermal fibroblast- neonatal (HDFn) using the in vitro PrestoBlue® cell
viability assay. The HCT-116 cell line was most susceptible to the compounds and mixtures tested.
Triterpene 1 was most cytotoxic against HCT-116 and MCF-7 with IC50 values of 4.21 and 5.92 μg/mL,
respectively. Triterpene 2 and the mixture of 3a and 3b were highly anti-proliferative against HCT-116
cells (IC50 of 1.22 and 1.66 μg/mL, respectively) and moderately inhibitory against MCF-7 cells (IC50 of
16.51 and 23.97 μg/mL, respectively). The mixture of 4a and 4b exhibited high cytotoxicity against HCT-
116 cells (IC50 of 1.14 μg/mL). Compounds 1-4b showed the least activity against HT-29 cells (IC50 of
11.97 to 52.52 μg/mL). Cytotoxic effect was not observed against HDFn cells (>100 μg/mL). Comparing
the effects of 1-4b on the two colon cancer cell lines, the IC50 values of 1-4b against HCT-116 were lower
than those of HT-29.
Consolacion Y. Ragasa, Vincent Antonio S. Ng, Mariquit M. De Los Reyes, Emelina H. Mandia and
Chien-Chang Shen
Chemical constituents of Wrightia pubescens (R.Br.). Der Pharmacia Lettre, 2014, 6 (6):14-19
Abstract: Chemical investigation of the dichloromethane extracts of Wrightia pubescens (R.Br.) led to the
isolation of ursolic acid (1), oleanolic acid (2), squalene (3), β-sitosterol (4) and chlorophyll a (5) from the
leaves; and 1, 2 and α-amyrin acetate (6) from the twigs. The structures of 1-6 were identified by
comparison of their 1HNMR and/or 13CNMR data with those reported in the literature.
Consolacion Y. Ragasa, Virgilio Ebajo Jr., Mariquit M. De Los Reyes, Emelina H.Mandia, Robert Brkljača
and Sylvia Urban
Triterpenes from Calophyllum inophyllum Linn. International Journal of Pharmacognosy and
Phytochemical Research 2015; 7(4); 718-722 ISSN: 0975-4873
Abstract: Chemical investigation of the dichloromethane extracts of Calophyllum inophyllum Linn.
afforded friedelin (1), squalene (2), canophyllic acid (3), and a mixture of canophyllalic acid (4a) and
canophyllol (4b) from the leaves; and 1 and taraxasterol (5) from the twigs. The structures of 3-5 were
elucidated by extensive 1D and 2D NMR spectroscopy, while those of 1 and 2 were identified by
comparison of their NMR data with those reported in the literature.
Development of a Controller Board with On-board Sensors for Unmanned Rotor-Based Aerial
Vehicles
25 F U/S 1TAY14-1TAY15
Mr. Elmer Magsino
Project Direct Expense: Php 4,665.00
Abstract: Unmanned aerial vehicles (UAV) research has grown immensely over the past decades due to its
maneuverability and vast applications such as agriculture, photography, disaster monitoring, rescue and
operation and surveillance. A UAV in stable flight is always dependent on its controller board and on-
board sensors. The researchers aim to develop a controller board equipped with various sensors necessary
to achieve a stable flight for a rotor-based unmanned aerial vehicle with no more than eight rotors. Such
controller board must be able to control at most eight motors independently while simultaneously
acquiring, correlating, and logging data from various sensors. The raw data are processed in real-time and
produces the correct PWM duty cycle to obtain stable flight in terms of attitude and altitude
Completed Projects
Examining Cultural and Media Rituals in Advertising: A Semiotic Analysis of Television
Commercials on Mobile Services
35 F S 2TAY14-1TAY15
Dr. Cheryll Ruth Soriano
Project Direct Expense: Php 15,000.00 PUBLISHED PAPER:
Sun Sun Lim, Cheryll Soriano
Ritual and communal connection in mobile phone communication: Representation of kapwa, bayanihan
and People Power in the Philippines. Asian Perspective on Digital Culture: Emerging Phenomena,
Enduring Concepts by Routledge
Abstract (Description): In Asia, amidst its varied levels of economic development and diverse cultural
traditions and political regimes, the Internet and mobile communications are increasingly used in every
aspect of life. Yet the analytical frames used to understand the impact of digital media on Asia
predominantly originate from the Global North, neither rooted in Asia’s rich philosophical traditions, nor
reflective of the sociocultural practices of this dynamic region. This volume examines digital phenomena
and its impact on Asia by drawing on specifically Asian perspectives. Contributors apply a variety of Asian
theoretical frameworks including guanxi, face, qing, dharma and karma. With chapters focusing on
emerging digital trends in China, Hong Kong, India, Japan, Korea, Philippines, Singapore, and Taiwan, the
book presents compelling and diverse research on identity and selfhood, spirituality, social networking,
corporate image, and national identity as shaped by and articulated through digital communication
platforms.
Bioactivities of the Crude Dichloromethane and Aqueous Extracts and a New Triterpene from
Glinus oppositifolius
10 IR U/S 3TAY11-1TAY13
Dr. Consolacion Ragasa, Dr. Esperanza Cabrera, and Dr. Oscar Torres
Project Direct Expense: Php 73,059.34 PUBLISHED PAPER: