Factors Regulating Environmental Transformation of Organic P – Nutrient Resource Ratio By Sayantani Ghosh (51444937) MSc Environmental Microbiology Supervised by Professor Graeme Paton
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Factors Regulating Environmental Transformation of
Organic P – Nutrient Resource Ratio By Sayantani Ghosh (51444937)
MSc Environmental MicrobiologySupervised by Professor Graeme Paton
Introduction
• Phosphorus is the basis of all living forms which cannot be replaced and is a non-renewable resource.• 90% of global demand for phosphorus is directed to agriculture for its use as fertilizer in food production.
• The P present in the wastewater is a major threat to the environment. • Therefore, P recovery from wastewater system is likely to become a sustainable option to meet current and future P demands in agriculture.
Establish the relationship between P transformation andnutrient resource ratio. The main objective of the work was manipulation of nutrient resource ratio expecting to notice a change in P mineralization capacities.
The C:N:P ratio was manipulated to measure the difference of aryl – phosphatase activity and its effect on the biomass and pH .
Methods
Samples were taken each time to determine the effect of the varied ratio on the aryl-phosphatase activity. The phosphatase assay was used but in a microtiter plate so that a large number of samples could be analysed over a short period of time.
Skimmed milk was used as the source of organic P where the inoculum was added and C:N ratio was varied and placed on the shaker incubator to carry on the experiment.
The samples were also digested by acetic acid and analysed using FIA (flow injection analysis) to determine the production of PO4
3- .
The cfu and the pH was also calculated to determine its effect.
Results
Fig. 1 • C:N 9.2:.5; • C:N 18.4:.5; • C:N 23:.5; • 46:.5
7.4 7.6 7.8 8 8.2 8.4 8.6 8.8 90
1000000000
2000000000
3000000000
4000000000
5000000000
6000000000
7000000000
mM pnp /hr
colo
ny fo
rmin
g un
its/m
l
7.4 7.6 7.8 8 8.2 8.4 8.6 8.8 947
48
49
50
51
52
53
54
55
56
mM PNP/HR
PO43
-
Fig. 1 • C:N 9.2:.5; • C:N 18.4:.5; • C:N 23:.5; • 46:.5
Results
11.5 12.5 13.5 14.5 15.5 16.5 17.5 18.50.00E+00
1.00E+09
2.00E+09
3.00E+09
4.00E+09
5.00E+09
6.00E+09
7.00E+09
mM pnp/hr
colo
ny fo
rmin
g un
its/m
l
10 11 12 13 14 15 16 17 18 19 2046
48
50
52
54
56
58
60
62
mM PNP/HR
PO43
-
Fig. 1 • C:N 4.6:1; • C:N 4.6:2 • C:N 4.6:2.5; • 4.6:5
Fig. 1 • C:N 4.6:1; • C:N 4.6:2 • C:N 4.6:2.5; • 4.6:5
Future prospects
It was seen that the assay could be performed in a microtiter plate if possible modifications are done accordingly.In the future, the efficiency of the microtiter plate method can be tested at commercial level and with wastewater samples.Comparing the results of different C:N ratios, the appropriate ratio for the maximum activity will be challenging to determine as the wastewater will have highly varied C:N ratios.
ASHLEY, K., CORDELL, D. and MAVINIC, D., 2011. A brief history of phosphorus: From the philosopher’s stone to nutrient recovery and reuse. Chemosphere, 84(6), pp. 737-746.
References
KARUNANITHI, R., SZOGI, A.A., BOLAN, N., NAIDU, R., LOGANATHAN, P., HUNT, P.G., VANOTTI, M.B., SAINT, C.P., OK, Y.S. and KRISHNAMOORTHY, S., Phosphorus Recovery and Reuse from Waste Streams. Advances in Agronomy, (0).
GATERELL, M., GAY, R., WILSON, R., GOCHIN, R. and LESTER, J., 2000. An economic and environmental evaluation of the opportunities for substituting phosphorus recovered from wastewater treatment works in existing UK fertiliser markets. Environmental technology, 21(9), pp. 1067-1084.
TABATABAI, M.A. and BREMNER, J.M., 1969. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, 1(4), pp. 301-307.
Thank you
Related Documents
