SANTA CRUZ BIOTECHNOLOGY, INC. cyclin E (HE12): sc-247 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND Cyclins were first identified in invertebrates as proteins that oscillate dramati- cally through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G 1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G 1 /S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally reg- ulated manner. The cyclin E/E2F complex is detected primarily during the G 1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins. CHROMOSOMAL LOCATION Genetic locus: CCNE1 (human) mapping to 19q12; Ccne1 (mouse) mapping to 7 B2. SOURCE cyclin E (HE12) is a mouse monoclonal antibody raised against recombinant cyclin E of human origin. PRODUCT Each vial contains 200 μg IgG 1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. cyclin E (HE12) is available conjugated to either fluorescein (sc-247 FITC), Alexa Fluor ® 546 (sc-247 AF546) or Alexa Fluor ® 594 (sc-247 AF594), 200 μg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either Alexa Fluor ® 680 (sc-247 AF680) or Alexa Fluor ® 790 (sc-247 AF790), 200 μg/ml, for Near-Infrared (NIR) WB, IF and FCM. In addition, cyclin E (HE12) is available conjugated to TRITC (sc-247 TRITC, 200 μg/ml), for IF, IHC(P) and FCM. Alexa Fluor ® is a trademark of Molecular Probes, Inc., Oregon, USA APPLICATIONS cyclin E (HE12) is recommended for detection of cyclin E (doublet at 50 kDa and single band at 42 kDa) of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunopre- cipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500); Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Suitable for use as control antibody for cyclin E siRNA (h): sc-29288, cyclin E siRNA (m): sc-29289, cyclin E shRNA Plasmid (h): sc-29288-SH, cyclin E shRNA Plasmid (m): sc-29289-SH, cyclin E shRNA (h) Lentiviral Particles: sc-29288-V and cyclin E shRNA (m) Lentiviral Particles: sc-29289-V. Molecular Weight of cyclin E: 53 kDa. Positive Controls: HeLa whole cell lysate: sc-2200, MEG-01 cell lysate: sc-2283 or JAR cell lysate: sc-2276. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. DATA SELECT PRODUCT CITATIONS 1. Zalcman, G., et al. 1995. Regulation of Ras-related RhoB protein expres- sion during the cell cycle. Oncogene 10: 1935-1945. 2. Smith, E., et al. 1995. Expression of cell cycle regulatory factors in differ- entiating osteoblasts: postproliferative up-regulation of cyclins B and E. Cancer Res. 55: 5019-5024. 3. Guo, X.W., et al. 1995. Chromosome condensation induced by fostriecin does not require p34cdc2 kinase activity and histone H1 hyperphosphoryla- tion, but is associated with enhanced histone H2A and H3 phosphorylation. EMBO J. 14: 976-985. 4. Zhang, M.Y., et al. 2019. Effects of Beclin 1 overexpression on aggres- sive phenotypes of colon cancer cells. Oncol. Lett. 17: 2441-2450. 5. Xue, Y., et al. 2019. SMARCA4 loss is synthetic lethal with CDK4/6 inhibi- tion in non-small cell lung cancer. Nat. Commun. 10: 557. 6. Xue, Y., et al. 2019. CDK4/6 inhibitors target SMARCA4-determined cyclin D1 deficiency in hypercalcemic small cell carcinoma of the ovary. Nat. Commun. 10: 558. 7. Cornell, L., et al. 2019. MicroRNA-mediated suppression of the TGF-b pathway confers transmissible and reversible CDK4/6 inhibitor resist- ance. Cell Rep. 26: 2667-2680.e7. 8. Zhang, X., et al. 2019. Regulation of OLC1 protein expression by the anaphase-promoting complex. Oncol. Lett. 17: 2639-2646. 9. Schade, A.E., et al. 2019. Cyclin D-CDK4 relieves cooperative repression of proliferation and cell cycle gene expression by DREAM and RB. Oncogene 38: 4962-4976. RESEARCH USE For research use only, not for use in diagnostic procedures. cyclin E (HE12): sc-247. Immunofluorescence stain- ing of methanol-fixed HeLa cells showing nuclear localization (A). Immunoperoxidase staining of for- malin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts and cytoplasmic staining of Leydig cells (B). B A cyclin E (HE12): sc-247. Western blot analysisof cyclin Eexpression in HeLa (A), MEG-01(B), JAR (C), MOLT-4 (D), IMR-32 (E) and MCF7 (F) whole cell lysates. 55 K – 43 K – 34K – 23 K – A B C D E F < cyclin E