Ryan S. Senger - Senger Lab - Home S Senger CV updated 20161222.pdf · Ryan S. Senger Associate Professor Department of Biological Systems Engineering Department of Chemical Engineering

Curriculum Vitae

Ryan S. Senger Associate Professor Department of Biological Systems Engineering Department of Chemical Engineering Virginia Tech 301C HABB1 1230 Washington St. Blacksburg, VA 24061 USA Office Phone: 540-231-9501 Email: [email protected] Website: http://www.mesb.bse.vt.edu RESEARCH INTERESTS

My lab performs experimental and computational research in the areas of (i) metabolic engineering, (ii) systems biology, and (iii) real-time physiological monitoring. One focus of our research is to produce valuable chemicals, pharmaceuticals, and biofuels from microbes, cell-free enzymatic pathways, and plants. We also focus on making advances using Raman spectroscopy and surface-enhanced Raman scattering (SERS) to (i) monitor physiological changes of cells, tissues, and organs in real-time and (ii) determine the chemical composition of sub-cellular locations and organelles. As the co-founder and Chief Technology Officer of DialySensors, Inc., I am interested in transitioning our technologies to applications with commercial relevance. DialySensors, Inc. has patented technology to detect chronic kidney disease as well as bladder and urinary tract cancers using a simple, inexpensive, and non-invasive analysis of urine by Raman spectroscopy. The technology is also used to monitor the health and progress of patients undergoing hemodialysis and peritoneal dialysis therapies.

EDUCATION

Ph.D., 2005, Chemical Engineering, Colorado State University (USA) Advisor: M. Nazmul Karim M.S., 2002, Chemical Engineering, Colorado State University (USA) Undergraduate Chemical Engineering Core Curriculum, 1999-2000 Colorado State University (USA) B.S., 1999, Chemistry, Millikin University (Decatur, IL USA)

POSITIONS

Associate Professor (Courtesy Appointment), 2/2016-Present Dept. of Chemical Engineering, Virginia Tech (USA) Associate Professor, 8/2015-Present Dept. of Biological Systems Engineering, Virginia Tech (USA) Chief Technology Officer, 7/2014-Present DialySensors, Inc., Floyd, VA (USA)

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Assistant Professor, 1/2009-8/2015 Dept. of Biological Systems Engineering, Virginia Tech (USA)

NIH Ruth L. Kirschstein NRSA Postdoctoral Fellow, 5/2007-12/2008 Dept. of Chemical Engineering, University of Delaware (USA) (10/2007-12/2008) Dept. of Chemical and Biological Eng., Northwestern University (USA) (5/07-10/07) Project Title: Clostridial Genome-Scale Metabolic and Regulatory Model of Differentiation Sponsor: Eleftherios (Terry) Papoutsakis Postdoctoral Researcher, 2/2006-5/2007 Dept. of Chemical and Biological Engineering, Northwestern University (USA) Advisor: Eleftherios (Terry) Papoutsakis Postdoctoral Researcher, 2/2005-2/2006 Dept. of Chemical Engineering, Texas Tech University (USA) Advisor: M. Nazmul Karim

AWARDS

Outstanding Faculty Member, Department of Biological Systems Engineering Alpha Epsilon Honor Society, 2013, 2016

Outstanding New Faculty Award, College of Engineering at Virginia Tech, 2012 PROFESSIONAL SOCIETIES

American Institute of Chemical Engineers (AIChE) American Chemical Society (ACS) American Association for the Advancement of Science (AAAS) American Society for Engineering Education (ASEE) American Society for Microbiology (ASM) American Society of Agricultural and Biological Engineers (ASABE) Institute of Biological Engineers (IBE)

PEER-REVIEWED JOURNAL PUBLICATIONS (34)

Google Scholar h-index (as of December 2016) = 14 Number of Citations (as of December 2016) = 826 * Indicates advisee

(1) Olson, M.L., Johnson, J., Carswell*, W.F., Reyes, L.H., Senger, R.S., Kao, K.C. (2016)

Characterization of an evolved carotenoids hyper-producer of Saccharomyces cerevisiae through bioreactor parameter optimization and Raman spectroscopy. Journal of Industrial Microbiology & Biotechnology. 43(10):1355-1363. Doi:10.1007/s10295-016-1808-9. (2015 impact factor = 2.745)

(2) Freedman*, B.G., Zu*, T.N.K., Athamneh*, A.I.M., Wallace*, R.S., Senger, R.S. (2016) Raman spectroscopy detects phenotypic differences among E. coli enriched for 1-butanol tolerance using a metagenomic DNA library. Biotechnology Journal. 11(7):877-889. Doi:10.1002/biot.201500144. (2105 impact factor = 3.781)

(3) Zu*, T.N.K., Athamneh*, A.I.M., Senger, R.S. (2016) Characterizing the phenotypic responses of Escherichia coli to multiple 4-carbon alcohols with Raman spectroscopy. Fermentation. 2(1):3. doi:10.3390/fermentation2010003. (2015 impact factor = N/A)

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(4) Nazem-Bokaee*, H., Senger, R.S. (2015) ToMI-FBA: A genome-scale metabolic flux based algorithm to select optimum hosts and media formulations for expressing pathways of interest. AIMS Bioengineering. 2(4):355-73. doi:10.3934/bioeng.2015.4.335. (2015 impact factor = N/A)

(5) Ebrahim, A., Almass, E., Bauer, E., Bordbar, A., Burgard, A.P., Chang, R.L., Drager, A., Famili, I., Feist, A.M., Fleming, R.M.T., Fong, S.S., Hatzimanikatis, V., Herrgard, M.J., Holder, A., Hucka, M., Hyduke, D., Jamshidi, N., Lee, S.Y., Le Novere, N., Lerman, J.A., Lewis, N.E., Ma, D., Mahadevan, R., Maranas, C. Nagarajan, H., Navid, A., Nielsen, J., Nielsen, L.K., Nogales, J., Noronha, A., Pal, C., Palsson, B.O., Papin, J.A., Patil, K.R., Price, N.D., Reed, J.L., Saunders, M., Senger, R.S., Sonnenschein, N., Sun, Y., Thiele, I. (2015) Do genome-scale models need exact solvers or clearer standards? Molecular Systems Biology. 11(10):831. doi:10.15252/msb.20156157. (2015 impact factor = 10.5)

(6) Rollin, J.A., Martin del Campo, J., Myung, S., Sun, F., Bakovic, A.E., Castro, R.L., Candrayan, S., Adams, M.W.W., Senger, R.S., Zhang, Y.H.P. (2015) High-yield enzymatic hydrogen production from biomass by in vitro metabolic engineering. Proceedings of the National Academy of Sciences USA. 112(16):4964-9. doi:10.1073/pnas.1417719112. (2015 impact factor = 9.423)

(7) Zu*, T.N.K., Athamneh*, A.I.M., Collakova, E., Robertson, J., Hawken, T., Aardema, C., Senger, R.S. (2015) Assessment of ex vivo perfused liver health by Raman spectroscopy. Journal of Raman Spectroscopy. 46:551-8. doi:10.1002/jrs.4688 (2015 impact factor = 2.395)

(8) Yen*, J.Y., Tanniche*, I., Fisher*, A.K., Bevan, D.R., Gillaspy, G.E., Senger, R.S. (2014) Designing metabolic engineering strategies with genome-scale metabolic flux modeling. Advances in Genomics and Genetics. 5:93-105. doi:10.2147/AGG.S58494. (2014 impact factor = N/A)

(9) Zu*, T.N.K., Athamneh*, A.I.M., Collakova, E., Senger, R.S. (2014) Near real-time analysis of the phenotypic responses of Escherichia coli to 1-butanol exposure using Raman spectroscopy. Journal of Bacteriology. 196, 3983-3931. doi:10.1128/JB.01590-14. (2014 impact factor = 2.808)

(10) Fisher*, A.K., Freedman*, B.G., Bevan, D.R., Senger, R.S. (2014) A review of metabolic and enzymatic engineering strategies for designing and optimizing performance of microbial cell factories. Computational and Structural Biotechnology Journal. 11, 91-9. doi:10.1016j/j.csbj.2014.08.010. (2014 impact factor = N/A)

(11) Senger, R.S., Yen*, J.Y., Fong, S.S. (2014) A review of genome-scale metabolic flux modeling of anaerobiosis in biotechnology. Current Opinion in Chemical Engineering. 6, 33-42. doi:10.1016/j.coche.2014.08003. (2015 impact factor = 3.571)

(12) Shin, J.H., Wakeman, C.A., Rodionov, D.A., Goodson, J.R., Freedman*, B.G., Senger, R.S., Winkler, W.C. (2014) Transport of magnesium by a bacterial NRAMP. PLoS Genetics. 10, e1004429. doi:10.1371/journal.pgen.1004429. (2013 impact factor = 8.167)

(13) Apte*, A.A., Senger, R.S., Fong, S.S. (2014) Designing novel cellulase systems through agent-based modeling and global sensitivity analysis. Bioengineered. 5, 436-446. doi:10.4161/bioe.29160. (2014 impact factor = N/A)

(14) Athamneh*, A.I., Alajlouni, R.A., Seleem, M.N., Senger, R.S. (2014) Identifying the phenotypic response of bacteria to antibiotic treatment using Raman spectroscopy. Antimicrobial Agents and Chemotherapy. 58, 1302-14. doi:10.1128/AAC.02098-13. (2014 impact factor = 4.980)

(15) Senger, R.S., Nazem-Bokaee*, H. (2013) Resolving cell composition through simple measurements, genome-scale modeling, and a genetic algorithm. Methods in Molecular Biology. 985, 85-101. doi:10.1007/978-1-62703-299-5_5. (2013 impact factor =N/A)

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(16) Yen*, J.Y., Nazem-Bokaee*, H., Freedman*, B.G., Athamneh*, A.I., Senger, R.S. (2013) Deriving metabolic engineering strategies from genome-scale modeling with flux ratio constraints. Biotechnology Journal. 8, 581-594. doi:10.1002/biot.201200234. (2013 impact factor = 3.708)

(17) Athamneh*, A.I., Senger, R.S. (2012) Peptide-guided surface-enhanced Raman scattering probes for localized cell composition analysis. Applied and Environmental Microbiology. 78, 7805-8. doi:10.1128/AEM.02000-12. (2012 impact factor = 4.406)

(18) Collakova, E., Yen*, J.Y., Senger, R.S. (2012) Are we ready for genome-scale modeling in plants? Plant Science. 191-2, 53-70. doi:10.1016/j.plantsci.2012.04.010. (2012 impact factor = 2.997)

(19) McAnulty*, M.J., Yen*, J., Freedman*, B.G., Senger, R.S. (2012) Genome-scale modeling using flux ratio constraints to enable metabolic engineering of clostridial metabolism in silico. BMC Systems Biology. 6, 42. doi:10.1186/1752-0509-6-42. (2012 impact factor = 3.300)

(20) Milne, C.B., Eddy, J.A., Raju, R., Ardekani, S., Kim, P.J., Senger, R.S., Jin, Y.S., Blaschek, H.P., Price, N.D. (2011) Metabolic network reconstruction and genome-scale model of butanol-producing strain Clostridium beijerinckii NCIMB 8052. BMC Systems Biology. 5, 130. doi:10.1186/1752/0509-5-130. (2011 impact factor = 3.544)

(21) Reed, J.L., Senger, R.S., Antoniewicz, M.R., Young, J.D. (2010) Computational approaches in metabolic engineering. Journal of Biomedicine and Biotechnology. 2010, 207414. doi:10.1155/2010/207414. (2010 impact factor = 1.742)

(22) Ogejo, J.A., Senger, R.S., Zhang, R. (2010) Global sensitivity analysis for a process based model for ammonia emissions from manure storage and treatment structures. Atmospheric Environment. 44, 3621-3629. doi:10.1016/j.atmosenv.2010.06.053. (2010 impact factor = 3.436)

(23) Senger, R.S. (2010) Improving biofuels production with genome-scale models: The role of cell composition. Biotechnology Journal. 5, 671-85. doi:10.1002/biot.201000007. (2012 impact factor = 3.446)

(24) Wang, M., Senger, R.S., Paredes, C.J., Banik, G.G., Lin, A., Papoutsakis, E.T. (2009) Microarray-based gene expression analysis as a process characterization tool to help establish comparability of complex biological products: the scale-up of a whole-cell immunotherapy product. Biotechnology and Bioengineering. 104, 796-808. doi:10.1002/bit.22441. (2009 impact factor = 3.627)

(25) Senger, R.S., Papoutsakis, E.T. (2008) Genome-scale model for Clostridium acetobutylicum: Part II. Development of specific proton flux states and numerically determined sub-systems. Biotechnology and Bioengineering. 101, 1053-71. doi:10.1002/bit.22009. (2008 impact factor = 3.255)

(26) Senger, R.S., Papoutsakis, E.T. (2008) Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis. Biotechnology and Bioengineering. 101, 1036-52. doi:10.1002/bit.22010. (2008 impact factor = 3.255)

(27) Jones, S.W., Paredes, C.J., Tracy, B., Cheng, N., Sillers, R., Senger, R.S., Papoutsakis, E.T. (2008) The transcriptional program underlying the physiology of clostridial sporulation. Genome Biology. 9, R114. doi:10.1186/gb-2008-9-7-r114. (2008 impact factor = 7.812)

(28) Senger, R.S., Karim, M.N. (2008) Prediction of N-linked glycan branching patterns using artificial neural networks. Mathematical Biosciences. 211, 89-104. (2008 impact factor = 1.526)

(29) Paredes, C.J., Senger, R.S., Spath, I.S., Borden, J.R., Sillers, R., Papoutsakis, E.T. (2007) A general framework for designing and validating oligomer-based DNA microarrays and its application to Clostridium acetobutylicum. Applied and Environmental Microbiology. 73, 4631-8. (2007 impact factor = 4.720)

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(30) Senger, R.S., Karim, M.N. (2007) Optimization of fed-batch parameters and harvest time of CHO cell cultures for a glycosylated product with multiple mechanisms of inactivation. Biotechnology and Bioengineering. 98, 378-90. (2007 impact factor = 3.276)

(31) Senger, R.S., Phisalaphong, M., Karim, M.N., Linden, J.C. (2006) Development of a culture sub-population induction model: signaling pathways synergy and taxanes production by Taxus canadensis. Biotechnology Progress. 22, 1671-82. (2006 impact factor = 2.102)

(32) Senger, R.S., Karim, M.N. (2005) Variable site-occupancy classification of N-linked glycosylation using artificial neural networks. Biotechnology Progress. 21, 1653-62. (2005 impact factor = 1.985)

(33) Senger, R.S., Karim, M.N. (2003) Neural-network-based identification of tissue-type plasminogen activator protein production and glycosylation in CHO cell culture under shear environment. Biotechnology Progress. 19, 1828-36. (2003 impact factor = 1.488)

(34) Senger, R.S., Karim, M.N. (2003) Effect of shear stress on intrinsic CHO culture state and glycosylation of recombinant tissue-type plasminogen activator protein. Biotechnology Progress. 19, 1199-209. (2003 impact factor = 1.488)

PEER-REVIEWED BOOK CHAPTERS (3)

(35) Apte*, A.A., Fong, S.S., Senger, R.S. (2013) Using cellular automata and global sensitivity analysis to study the regulatory network of the L-arabinose operon, in Emerging Applications of Cellular Automata (A. Salcido Ed.), InTech. ISBN: 978-953-51-1101-6.

(36) Freedman*, B.G., Athamneh*, A.I.M., McAnulty*, M.J., Zhou*, R., Yue*, Y., Senger, R.S. (2014) Metabolic engineering the clostridia for biobutanol production. Accepted book chapter in press.

(37) Paredes, C.J., Jones, S.W., Senger, R.S., Borden, J.R., Sillers, R., Papoutsakis, E.T. (2008) Molecular aspects of butanol fermentation, in Bioenergy: Microbial contributions to alternative fuels (Wall, J.D., Harwood, C.S., Demain, S., Eds.), American Society for Microbiology Press.

MANUSCRIPTS UNDER REVIEW AND IN REVISION (4)

(38) Nazem-Bokaee*, H., Yen*, J.Y., Athamneh*, A.I.M., Apte*, A.A., McAnulty*, M.J., Senger, R.S. (2016) SyM-GEM: A synthetic metabolic pathway builder and genome-scale model database to aid systems metabolic engineering in biotechnology. Submitted to Biotechnology Journal.

(39) Fisher*, A., Carswell*, W.F., Athamneh*, A.I., Bevan, D.R., Senger, R.S. (2016) The RDA Toolbox: A collection of user-friendly tools dedicated to handling, pre-processing, and analyzing Raman spectroscopy data. Submitted to Biotechnology Journal.

(40) Freedman*, B.G., Senger, R.S. (2016) Thermodynamic design of degenerate oligonucleotide primers for whole genome DNA amplification with reduced bias for use in DNA libraries. Submitted to DNA Research.

(41) Freedman*, B.G., Lee*, P.W., Senger, R.S. (2016) Engineering the metabolic profile of Clostridium cellulolyticum ATCC 35319 with genome DNA libraries. Submitted to Biotechnology Progress.

PATENTS (2)

(1) Robertson, J., Senger, R.S. “System for assessment of hemodialysis efficacy,” Virginia Tech Intellectual Properties (filed patent application in April 2015)

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(2) Senger, R.S., Papoutsakis, E.T. "Reverse engineering genome-scale metabolic network reconstructions for organisms with incomplete genome annotation and a completed network for the solventogenic Clostridium acetobutylicum ATCC 824," US Patent 8311790 B2

DISCLOSURES (3)

(1) Senger, R.S., Athamneh, A.I.M., Robertson, J. “Near real-time monitoring of ex vivo organ health and metabolic activity,” Virginia Tech Intellectual Properties (1/13/14).

(2) Senger, R.S. “A cellulolytic strain of Clostridium acetobutylicum,” Virginia Tech Intellectual Properties (9/22/2009)

(3) Senger, R.S. “FITSelect: A technology to enrich a genomic library to maximize product production using a growth competition assay,” Virginia Tech Intellectual Properties (9/22/2009)

RESEARCH FUNDING

TOTAL RESEARCH FUNDING AWARDED AS PRINCIPAL INVESTIGATOR: $2,074,734

TOTAL RESEARCH FUNDING ALLOCATED TO THE SENGER LAB: $1,816,747

(1) Senger, R.S. (PI), Bevan, D.R. (Co-PI) (10/1/2013-9/30/2016) “Production of the building block 2-pyrrolidone using a model-guided platform for de novo biosynthetic pathway integration.” NSF CBET BBBE Program, Award NSF1254242. Total Amount: $398,394. Senger Lab Amount: $275,353

(2) Senger, R.S. (PI), Collakova, E. (Co-PI) (3/1/2013-2/28/2016) “Enabling phenotype predictions of cyanobacteria” NSF MCB Networks and Regulation Program, Award: NSF1243988. Total Amount: $621,182. Senger Lab Amount: $310,591

(3) Senger, R.S. (PI), Bevan, D.R. (Co-PI) (9/30/2013-9/29/2014) “Evaluating a de novo biosynthetic pathway to make the valuable building block chemical 2-pyrrolidone.” Jeffress Trust Awards Program in Interdisciplinary Research. Total Amount: $100,000. Senger Lab Amount: $100,000

(4) Senger, R.S. (PI), Gillaspy G.E. (Co-PI) (7/1/2012-6/30/2014) “Model-guided metabolic engineering of plants” Institute for Critical Technologies and Applied Science. Total Amount: $95,939. Senger Lab Amount: $95,939

(5) Senger, R.S. (PI) (6/10/2012) “International Travel Grant to Metabolic Engineering IX Conference; Biarritz, France” Virginia Tech International Travel Supplemental Grant. Total Amount: $1,000. Senger Lab Amount: $1,000

(6) Senger, R.S. (PI), Bevan, D.R. (Co-PI) (7/1/2011-6/30/2013) “Generating environmentally-friendly novel biofuel molecules with synthetic biology” Institute for Critical Technologies and Applied Science. Total Amount: $120,000. Senger Lab Amount: $120,000

(7) Senger, R.S. (PI), Apte, A.A. (Co-PI) (4/1/2011-8/31/2012) “Enzymatic synthesis of high-value building blocks for commodity and specialty chemicals: A bottom-up approach” Virginia Tech Biodesign and Bioprocess Research Center. Total Amount: $45,225. Senger Lab Amount: $45,225

(8) Senger, R.S. (PI), Gillaspy, G.E. (Co-PI) (4/15/2010-8/31/2012) “Model-guided metabolic engineering of Arabidopsis for increased cellulose production” Virginia Tech Biodesign and Bioprocess Research Center. Total Amount: $64,528. Senger Lab Amount: $32,264

(9) Senger, R.S. (PI) (11/1/2009-6/1/2014) “Metabolic engineering of Clostridium cellulolyticum using a genome-scale model and combinatorial approaches.” USDA

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CSREES AFRI Biobased Products and Bioenergy Production Research Program, Award: 2010-65504-20346. Total Amount: $445,395. Senger Lab Amount: $445,395

(10) Senger, R.S. (PI) (4/15/2009-8/31/2012) “Photanol: Biosynthesis of alcohol biofuels from sunlight and CO2” Virginia Tech Biodesign and Bioprocess Research Center. Total Amount: $36,703. Senger Lab Amount: $36,703

(11) Senger, R.S. (PI) (1/1/2009-5/31/2011) “Engineering cellulose utilization by the biobutanol producer Clostridium acetobutylicum: Characterizing genetic regulation of the cellulosomal scaffolding protein” Virginia Tech Biodesign and Bioprocess Research Center. Total Amount: $63,021. Senger Lab Amount: $63,021

(12) Senger, R.S. (PI) (5/1/2007-12/31/2008) “Clostridial genome-scale metabolic and regulatory model of differentiation” NIH, NRSA Post-doctoral Fellowship (E.T. Papoutsakis, sponsor) Award: F32GM078947. Total Amount: $83,347

TOTAL RESEARCH FUNDING AWARDED AS CO-PRINCIPAL INVESTIGATOR: $644,963

(13) Barone, J. (PI), Senger, R.S. (Co-PI) (7/1/2016-6/30/2017) “Multi-scale metabolic modeling and engineering workshop” NSF CBET, Award: 1637780. Total Amount: $25,000. Senger Lab Amount: $12,500

(14) Kuhn, D. (PI), Stevens, A. (Co-PI), Senger, R.S. (Co-PI) (9/1/2016-8/31/2017) “Microbial protein substitution for fishmeal in aquaculture diets: Phase II+: Bacterial strain characterization, application, and validation” Industrially funded project. Total Amount: $126,662. Senger Lab Amount: $42,220

(15) Kuhn, D. (PI), Stevens, A. (Co-PI), Senger, R.S. (Co-PI) (7/1/2015-12/1/2015) “Microbial protein substitution for fishmeal in aquaculture diets: Phase I bacteria strain selection” Industrially funded project. Total Amount: $50,655. Senger Lab Amount: $16,885

(16) Schmale, D. (PI), Senger, R.S. (Co-PI) (5/1/2015-4/30/2016) “Enzymatic detoxification of deoxynivalenol” USDA-USWBSI. Total Amount: $39,908. Senger Lab Amount: $19,954

(17) Schmale, D. (PI), Senger, R.S. (Co-PI) (5/1/2014-4/30/2015) “Enzymatic detoxification of deoxynivalenol” USDA-USWBSI. Total Amount: $40,429. Senger Lab Amount: $20,214

(18) Barone, J.R. (PI), Senger, R.S. (Co-PI) (1/1/2014-7/1/2014) “Genetically encoded 3D printing (GET-Print)” Institute for Critical Technologies and Applied Science. Total Amount: $20,032. Senger Lab Amount: $0

(19) Collakova, E. (PI), Senger, R.S. (Co-PI) (4/1/2011-3/30/2013) “Predicting high-oil phenotypes for seed rational metabolic engineering using genome-scale modeling” Virginia Tech Biodesign and Bioprocess Research Center. Total Amount: $42,266. Senger Lab Amount: $21,133

(20) Agblevor, F.A. (PI), Zhang, C. (Co-PI), Senger, R.S. (Co-PI) (1/1/2010-12/31/2012) “Virginia Tech – National Engineering School of Sfax partnership: Towards strengthening higher education & research capacities for better serving the economic development priorities in Tunisia” US State Department ESF Grants Program, Award: S-TS800-09-GR-204. Total Amount: $300,011. Senger Lab Amount: $75,003

NUMBER OF PENDING RESEARCH PROPOSALS: 5

TOTAL RESEARCH FUNDING REQUESTED IN PENDING PROPOSALS: $8,225,795

TOTAL RESEARCH FUNDING AWARDED TO DIALYSENSORS, INC.: $150,000

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(1) Robertson, J. (PI), Senger, R.S. (Co-PI) (6/1/2016-5/31/2017) “Redefining the management of renal disease through Chemometric FingerprintingTM of urine and dialysate” VT Knowledgeworks Innovation Challenge. Total Amount: $100,000

(2) Robertson, J. (PI), Senger, R.S. (Co-PI) (6/15/2015-6/15/2016) “DialysensingTM: Improving the efficacy and patient outcomes of hemodialysis and peritoneal dialysis through the use of Raman spectroscopy and multivariate statistical analysis” CIT CRCF CP15-015-LS. Total Amount: $50,000. Match Amount: $58,000

COMPUTER PROGRAMMING LANGUAGES

MATLAB, Visual Basic, C/C++, Perl, Python, PHP, HTML, MySQL WEBSITE PORTFOLIO

(1) Research group website: http://www.mesb.bse.vt.edu/ (2) SyM-GEM web application and MySQL database: http://www.mesb.bse.vt.edu/SyM-

GEM (Password protected while manuscript under review; Guest login name: hosting.guest; Password: guest123!)

(3) Freezer193 MySQL database: http://www.mesb.bse.vt.edu/Freezer193 (Under Construction; Guest login name: hosting.guest; Password: guest123!)

(4) BSE Department opportunities website and MySQL database: http://www.opportunities.bse.vt.edu

EDITORSHIP

Guest Editor, Frontiers in Microbiology, “Novel Metabolic Engineering Approaches for Producing Chemicals,” special edition to be published in 2017.

Guest Editor, Journal of Biomedicine and Biotechnology, “Computational Approaches in Metabolic Engineering,” special edition published in 2010.

REVIEWER

Journals: AIChE Journal, Applied Microbiology and Biotechnology; ASABE Publications; Biochemical Engineering Journal; Biological Engineering Transactions; Bioprocess and Biosystems Engineering; Bioresource Technology; Biotechnology & Bioengineering; Biotechnology and Bioprocess Engineering; Biotechnology for Biofuels; Biotechnology Journal; BMC Systems Biology; Current Opinion in Biotechnology; Industrial & Engineering Chemistry Research; Industrial Biotechnology; Integrative Biology; Journal of Biological Engineering; Marine Drugs; Metabolic Engineering; Metabolic Engineering Communications; Microbial Cell Factories; Nature Biotechnology; Nature Communications; PLoS Computational Biology; PLoS ONE; PNAS; Transactions in Biomedical Engineering; Transactions on Computational Biology and Bioinformatics

Books: Wiley; Elsevier

COMMITTEES

Advisory and Chair Positions: Assistant Department Head, Biological Systems Engineering Dept., 2015

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Bioprocessing Laboratory Manager Hiring Committee (Chair), Biological Systems Engineering Dept., 2014

Student Chapter (Faculty Advisor), American Society of Agricultural and Biological Engineers (ASABE), 2010-14

Southeastern Regional Organization (Faculty Advisor), American Society of Agricultural and Biological Engineers (ASABE) Southeastern Regional Organization, 2011-12

Potential Employer Committee (Chair), Biological Systems Engineering Dept., 2010-17 Teaching Evaluation Committee (Chair), BSE 4604 (Food Process Engineering), 2011 Teaching Evaluation Committee (Chair), BSE 2004 (Intro to BSE), 2016

Contributing Member: Academic Coordinator Hiring Committee, Biological Systems Engineering Dept., 2013-14 Curriculum Committee, Biological Systems Engineering Department, 2010-17 Dean Search Committee, Virginia Tech College of Engineering, 2016 Department Head Search Committee, Biological Systems Engineering Dept. 2010-11 Department Head Advisory Committee, Biological Systems Engineering Dept. 2015-16 Diversity Committee, Biological Systems Engineering Department, 2016-17 Faculty Advisor for iGEM, Team consisting of students and faculty from Virginia Tech,

University of Virginia, and Virginia Commonwealth University, 2010 Faculty Advisory Board, Institute for Critical Technologies and Applied Science, 2012-16 Faculty Candidate Search Committee (3), Biological Systems Engineering Dept. 2012-14 Graduate Committee, Biological Systems Engineering Department, 2009-12 Laboratory Modifications Committee, Biological Systems Engineering Dept., 2010-11 Laboratory Space Committee, Biological Systems Engineering Dept., 2010-11 Pilot Lab Management Committee, Biological Systems Engineering Dept., 2014-17 Promotion and Tenure Committee, Biological Systems Engineering Dept., 2015-17 Research Project Review Committee, Virginia Agricultural Experiment Station, 2013 Scholarship Committee, Biological Systems Engineering Department, 2012-17 Teaching Evaluation Committee, PPWS 5534 (Advanced Plant Physiology and Metabolism

II), 2012 Teaching Evaluation Committee, BSE 2104 (Numerical Methods), 2011 Teaching Evaluation Committee, BSE 4544/5544G (Protein Separations Engineering), 2010 Teaching Evaluation Committee, BSE 3524 (Unit Operations Laboratory), 2009

Professional Meetings: Southeastern Regional Rally Conference (Organizer), American Society of Agricultural and

Biological Engineers (ASABE), 2012 Biobased Fuels and Chemicals Session (Chair), American Institute of Chemical Engineers

(AIChE) Annual Meeting, 2011, 2015 Biobased Fuels and Chemicals Session (Co-Chair), American Institute of Chemical

Engineers (AIChE) Annual Meeting, 2012, 2014 Biobased Fuels and Chemicals I Session (Chair), American Institute of Chemical

Engineers (AIChE) Annual Meeting, 2013 Biobased Fuels and Chemicals III Session (Co-Chair), American Institute of Chemical

Engineers (AIChE) Annual Meeting, 2013 Cellular Models I Session (Co-Chair), American Institute of Chemical Engineers (AIChE)

Annual Meeting, 2013 Cellular Models II Session (Chair), American Institute of Chemical Engineers (AIChE)

Annual Meeting, 2013

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Metabolic Pathway Engineering Session (Chair), Institute of Biological Engineering (IBE) International Conference, 2012-13

INTERNATIONAL ACTIVITIES

As Co-PI of a U.S. State Department funded collaboration, I helped develop a synergistic relationship between the Virginia Tech Biological Systems Engineering Department and the National Engineering School of Sfax (Tunisia). This effort developed a dual-degree (M.S.) program between these universities. Through this program, I visited Tunisia to recruit students for the program, taught courses through distance learning, and hosted Tunisian students at Virginia Tech to advise research projects and share laboratory resources.

COURSES DEVELOPED AND TAUGHT

Instructor, Metabolic Engineering (BSE 4564) and Adv. Metabolic Engineering (BSE 5564G) Semesters Taught: Spring 2017 Senior/Graduate-Level Course in Biological Systems Engineering, Virginia Tech Course was developed with Prof. Xueyang Feng of Biological Systems Engineering Instructor, Metabolic Engineering (BSE 5984) Semesters Taught: Spring 2010 Graduate-Level Course in Biological Systems Engineering, Virginia Tech Instructor, Engineering Mathematics (ChE/BSE/SBES 5044) Semesters Taught: Fall 2010, Fall 2012, Fall 2016 Graduate-Level Course in Chemical Engineering (ChE)/Biological Systems Engineering (BSE)/School of Biomedical Engineering and Sciences (SBES), Virginia Tech Course was developed and taught with faculty in ChE and SBES.

OTHER COURSES TAUGHT

Instructor, Bioprocess Engineering (BSE 4504/5504G) Semesters Taught: Fall 2009-14 Senior/Graduate-Level Course in Biological Systems Engineering, Virginia Tech Course was significantly re-designed in Fall 2009. Instructor, Biological Process Plant Design (BSE 4524) Semesters Taught: Spring 2011-15, Fall 2015-16 Senior-Level Course in Biological Systems Engineering, Virginia Tech Course was significantly re-designed in Spring 2011 Instructor, Engineering Analysis for Biological Systems (Numerical Methods) (BSE 3144) Semesters Taught: Spring 2016 Sophomore-Level Course in Biological Systems Engineering, Virginia Tech Invited Lecturer, Paradigms for Bioinformatics (GBCB 5314) (Professors Ruth Greene and Eva Collakova) Semesters of Participation: Fall 2012 Graduate-level course in the Genomics, Bioinformatics, and Computational Biology Program, Virginia Tech

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Responsible for teaching one lecture on genome-scale metabolic flux modeling. Invited Lecturer, Introduction to Engineering (ENGR 1014) (Professor Cynthia Hampton) Semesters of Participation: Fall 2015 Freshman-level course in Engineering, Virginia Tech Responsible for contributing to one lecture on Biological Systems Engineering and Metabolic Engineering. Invited Lecturer, Industrial Processing (BSE 4514) (Professor Kumar Mallikarjunan) Semesters of Participation: Spring 2012 Senior-level course in Biological Systems Engineering, Virginia Tech Responsible for teaching a two-week section on biofuels. Invited Lecturer, Introduction to BSE (BSE 2004) (Professors Mike Zhang, Tess Thompson, Leigh-Anne Krometis) Semesters of Participation: Spring 2009, Fall 2010-2014 Sophomore-level course in Biological Systems Engineering, Virginia Tech Responsible for designing and implementing laboratory assignment and providing

accompanying lecture to sophomore-level BSE students. Invited Lecturer, Metabolic Engineering (Professor Eleftherios T. Papoutsakis) Semesters of Participation: Spring 2008 Graduate-level course in Chemical Engineering, University of Delaware Responsible for teaching a one-week section on metabolic modeling.

Co-Instructor, Biochemical Engineering (with Professor James C. Linden) Semesters of Participation: Fall 2004 Graduate-Level Course in Chemical Engineering, Colorado State University Responsible for writing lectures and teaching one-half of the semester.

RESEARCH TRAINING RECORD

Ph.D. Dissertations Directed (3): (1) Benjamin G. Freedman, Virginia Tech. Degenerate oligonucleotide primed amplification

of genomic DNA for combinatorial screening libraries and strain enrichment. 208 p. Ph.D. Biological Systems Engineering, November 2014

(2) Theresah N. K. Zu, Virginia Tech. Phenotypic and metabolic profiling of biological samples in near real-time using Raman spectroscopy. 156 p. Ph.D. Biological Systems Engineering, September 2014

(3) Hadi Nazem-Bokaee, Virginia Tech. Systems metabolic engineering through application of genome-scale metabolic flux modeling. 282 p. Ph.D. Biological Systems Engineering, April 2014

M.S. Theses Directed (5): (1) Jiun Yen, Virginia Tech (Co-advised). Systems metabolic engineering of Arabidopsis for

increased cellulose production. 134 p. M.S. Biological Systems Engineering, December 2013

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(2) Imen Tanniche, Virginia Tech. Correlating antisense RNA performance with thermodynamic calculations. 117 p. M.S. Biological Systems Engineering, December 2012

(3) Yang Yue, Virginia Tech. The development of a thermodynamic model for antisense RNA design and an electro-transformation protocol to introduce auxotrophic genes for enhancing eicosapentaenoic acid yield from Pythium irregular. 83 p. M.S. Biological Systems Engineering, December 2011

(4) Mike McAnulty, Virginia Tech. Total proton flux and balancing in genome-scale models: The case for the updated model of Clostridium acetobutylicum ATCC 824. 186 p. M.S. Biological Systems Engineering, May 2011

(5) Rui Zhou, Virginia Tech. FITSelect: An invention to select microbial strains maximizing product formation from a single culture without high-throughput screening. 87 p. M.S. Biological Systems Engineering, May 2011

Graduate Students in Progress (6):

(1) Jiun Yen, PhD (Co-advised), Systems Biology and Metabolic Engineering of Plants (2) Imen Tanniche, PhD, Phenotype Prediction and Engineering (3) Amanda Fisher, PhD (Co-advised), De Novo Biosynthetic Pathway Design (4) Nina Wilson, PhD (Co-advised), Gene Prospecting for Toxin Remediation (5) Pedro Ivo Guimaraes, PhD, De Novo Biosynthetic Pathway Design (6) Bill Carswell, PhD (Co-advised), Real-Time Monitoring with Raman Spectroscopy Postdoctoral Researchers and Visiting Scholars (2):

(1) Dr. Advait A. Apte, 2010-12 (2) Dr. Ahmad I.M. Athamneh, 2011-13

Undergraduate Researchers Trained (36): (1) Marisa Cole (VT, BSE), 8/2016-Present, “Detection of cancer with Raman spectroscopy” (2) Stephanie Lundgren (VT, BSE), 8/2016-Present, “Characterization of urine from healthy

human subjects” (3) Michelle Huynh (VT, Biochemistry), 8/2016-Present, “Monitoring short and long-term

changes in kidney function with Raman spectroscopy” (4) Meaghan Sullivan (VT, BSE), 5/2016-Present, “Monitoring dialysis efficiency with Raman

spectroscopy” (5) Austin Gouldin (VT, BSE), 8/2016-Present, “Detection of disease with Raman

spectroscopy” (6) Jihyeon Gong (VT, BSE), 5/2016-Present, “Real-time monitoring with Raman

spectroscopy” (7) Emily Berg (VT, BSE), 8/2016-Present, “Metabolic engineering of cyanobacteria” (8) Shelbie Dashiell (VT, BSE), 8/2016-12/2016, “An educational unit on fungal toxins” (9) Kendall Dunham (VT, BSE), 1/2016-5/2016, “Expressing proteins of a synthetic

metabolic pathway” (10) Derek Dunham (VT, BSE), 8/2015-5/2016, “Expressing proteins of a synthetic metabolic

pathway” (11) Jackson Toth (VT, BSE), 8/2015-8/2016, “Anaerobic digestion and synthetic biology” (12) Diego De La Torre Campos (VT, BSE), 8/20/15-Present, “Real-time monitoring with

Raman spectroscopy”

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(13) Ryan Frizzel (VT, BSE), 1/2015-7/2015, “Engineering fish waste digestion by cyanobacteria”

(14) Victoria Morales (Texas-Arlington, Aerospace Engineering), 6/2014-8/2014, “Engineering the cellulose-consuming Clostridium cellulolyticum H10 by media optimization and gene knockouts”

(15) Jessica Symons (Wisconsin-Platteville, Chemical Engineering), 6/2014-8/2014, “Determining mechanisms of toxicity using Raman spectroscopy”

(16) Manuel Martinez (VT, Chemical Engineering), 1/2014-12/2014, “Characterization of a clostridial cellulosome promoter”

(17) Mike Scimeca (VT, BSE), 1/2014-12/2014, “Municipal waste to fuel” (18) Mike Swartz (VT, BSE), 1/2014-5/2014, “Designing degenerate oligonucleotide primers

for producing genomic libraries” (19) Tiernan Mendes (Syracuse, Chemical Engineering), 6/2013-8/2013, “Designing

antisense RNA down-regulation” (20) Caitlyn Landin (University of Toledo, Chemical Engineering), 6/2013-8/2013,

“Combinatorial cloning of clostridial promoters” (21) Robert Wallace (VT, BSE), 1/2013-12/2013, “Genomic DNA library enrichment and

characterization by Raman spectroscopy” (22) Margaret Delany (VT, BSE), 8/2012-5/2013, “Micro-chemostat design and operation” (23) Stephanie Pagano (VT, BSE), 8/2012-5/2013, “Synthetic plasmid construction for

antisense RNA down-regulation studies” (24) Michelle Halsted (VT, BSE), 8/2012-5/2013, “Genomic library construction using

degenerate oligonucleotide priming” (25) Aya Youn (Syracuse, Chemical Engineering), 6/2012-8/2012, “Synthetic plasmid

construction methods” (26) Pharen Barron (North Carolina State University, Chemical Engineering), 6/2012-8/2012,

“Designing antisense RNA down-regulation” (27) Nick Mangili (VT, BSE), 1/2012-5/2012, “Designing antisense RNA down-regulation” (28) James Wade (VT, BSE), 6/2011-12/2011, “Synthetic biology in E. coli” (29) Sean Skinner (VT, BSE), 6/2011-8/2011, “Genomic libraries for combinatorial metabolic

engineering” (30) Tracy Twumasi (Virginia State University, Environmental Sciences), 6/2011-8/2011,

“Molecular cloning of clostridial promoters” (31) Parker Lee (VT, BSE), 1/2011-5/2012, “Genomic libraries for combinatorial metabolic

engineering” (32) Ashley Umberger (VT, BSE), 1/2011-5/2011, “Molecular cloning of clostridial promoters” (33) Jacob Carey (VT, BSE), 5/2010-12/2010, “Development of antisense RNA technologies” (34) Stephanie Wong (Cornell, Biological Engineering), 5/2010-8/2010, “Molecular cloning of

clostridial promoters” (35) Alexa Tenga (VT, BSE), 5/2009-5/2010, “Cloning and expressing clostridial enzymes in

E. coli” (36) Charlie Mayo (VT, BSE), 5/2009-12/2009, “Molecular cloning of clostridial promoters”

Research Associates and Laboratory Managers (1): (1) Yanjuan (Jill) Hong, 2009-10

INVITED LECTURES AND SEMINARS (34)

(1) Computational dialysis: Providing better patient outcomes (with John Robertson). Fresenius Kidney Care Crystal Springs. Roanoke, VA. September 29, 2016.

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(2) Conquering renal and bladder disease: A joint venture (with John Robertson). Virginia Tech Economic Development Group. Blacksburg, VA. July 8, 2016.

(3) Redefining the management of renal disease through “UrinFormaticsTM” of urine and dialysate (with John Robertson). Fresenius Kidney Care Crystal Springs. Roanoke, VA. June 23, 2016.

(4) Redefining the management of renal disease through Raman Metabolomics FingerprintingTM (R-omicsTM) of urine and dialysate (with John Robertson). Fresenius Kidney Care Crystal Springs. Roanoke, VA. April 21, 2016.

(5) Faculty-led startups and the VTIP experience: A case study (with John Robertson). Virginia Tech Intellectual Properties. Blacksburg, VA. February 23, 2016.

(6) An introduction to metabolic engineering. Virginia Governor’s School Summer Program. Blacksburg, VA. July 15, 2015.

(7) Computational dialysis: Providing better patient outcomes (with John Robertson). Valley Nephrology. Roanoke, VA. July 10, 2015.

(8) Computational dialysis: Providing better patient outcomes (with John Robertson). Wake Forest Medical School Department of Nephrology. Winston-Salem, NC. June 5, 2015

(9) Raman spectroscopy and genome-scale modeling are vital tools for metabolic engineering. North Carolina State University Department of Chemical Engineering. Raleigh, NC. November 3, 2014.

(10) Raman spectroscopy and genome-scale modeling are vital tools for metabolic engineering. Virginia Tech Interdisciplinary Program in Genomics Bioinformatics and Computational Biology. Blacksburg, VA. October 30, 2014.

(11) Salmon waste utilization. Institute for Critical Technologies and Applied Science (ICTAS) at Virginia Tech. Blacksburg, VA. October 7, 2014.

(12) Raman spectroscopy and genome-scale modeling are vital tools for metabolic engineering. Colorado State University Department of Chemical and Biological Engineering. Fort Collins, CO. September 26, 2014.

(13) Near real-time phenotyping of cells, tissues, and organs with Raman spectroscopy. Virginia Tech Microbiology REU Program. Blacksburg, VA. July 16, 2014.

(14) Engineering plants and microbial cell factories using new tools in genome-scale metabolic flux modeling and Raman spectroscopy. Clemson University Department of Chemical & Biomolecular Engineering. Clemson, SC. April 24, 2014.

(15) Engineering plants and microbial cell factories using new tools in genome-scale metabolic flux modeling and Raman spectroscopy. University of Maryland Department of Chemical and Biomolecular Engineering. College Park, MD. April 22, 2014.

(16) The potential employer resources in the Biological Systems Engineering Department at Virginia Tech. Virginia Tech American Society of Agricultural and Biological Engineers Chapter. Blacksburg, VA. February 2013.

(17) What is Biological Systems Engineering? Virginia Tech American Society of Agricultural and Biological Engineers Chapter. Blacksburg, VA. September 18, 2012.

(18) Engineering a BioEconomy with synthetic biology. Virginia Tech Department of Biological Systems Engineering. Blacksburg, VA. September 11, 2012.

(19) Engineering a BioEconomy with synthetic biology. Virginia Commonwealth University Department of Chemical and Life Sciences Engineering. Richmond, VA. April 18, 2012.

(20) Research in the metabolic engineering and systems biology laboratory at Virginia Tech. Virginia Tech Department of Biological Systems Engineering. Blacksburg, VA. October 25, 2011.

(21) The marriage of synthetic biology to metabolic engineering through systems biology and genome-scale modeling. Virginia Tech Interdisciplinary Program in Genomics, Bioinformatics, and Computational Biology. Blacksburg, VA. October 14, 2010.

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(22) New biofuels through synthetic biology and metabolic engineering. National Engineering School of Sfax. Sfax, Tunisia. September 27, 2010.

(23) The integration of metabolic engineering, systems biology, and synthetic biology for production of novel biofuels and chemicals. ICTAS Biobased Materials Research Center. Blacksburg, VA. September 24, 2010.

(24) Prediction of microbial cell composition using genome-scale models. Howard Hughes Medical Institute Summer Program at Virginia Commonwealth University. Richmond, VA. July 15, 2010.

(25) Whole-cell modeling: The case of biobutanol production using Clostridium acetobutylicum. Virginia United iGEM Team Bootcamp. Blacksburg, VA. June 4, 2010.

(26) The role of genomics in the biofuels revolution. Virginia Tech Biofuels Mini-Symposium. Blacksburg, VA. December 4, 2009.

(27) Metabolic engineering research in the Senger Laboratory. Virginia Tech Department of Biological Systems Engineering Advisory Board Meeting. Blacksburg, VA. October 23, 2009.

(28) Metabolic engineering research in the Senger Laboratory. Virginia Tech Department of Biological Systems Engineering. Blacksburg, VA. September 29, 2009.

(29) From biochemistry to systems biology: A look at genome-scale modeling. Millikin University Department of Chemistry Department Graduates of the Last Decade (GOLD) Seminar. Decatur, IL. May 2008.

(30) Aspects of genome-scale model development: The case of Clostridium acetobutylicum. Department of Biological Systems Engineering. Blacksburg, VA. March 2008.

(31) Reverse engineering a genome-scale model for Clostridium acetobutylicum. Delaware Biotechnology Institute Seminar Series. Newark, DE. March 2008.

(32) Variable site-occupancy classification of N-linked glycosylation using artificial neural networks. Northwestern University Department of Chemical and Biological Engineering (Papoutsakis Research Group). Evanston, IL. November 2005.

(33) Modeling of recombinant enzyme inactivation and prediction of N-linked glycosylation site-occupancy and microheterogeneity. Colorado State University Department of Chemical Engineering. Fort Collins, CO. January 2005.

(34) Effect of Shear Stress on Recombinant Tissue-Type Plasminogen Activator (r-tPA) Protein Production and Glycosylation from CHO Cell Culture (invited seminar) Colorado State University Department of Chemical Engineering. Fort Collins, CO. October 2002.

PROFESSIONAL MEETING PAPERS AND PRESENTATIONS (107)

*Indicates advisee

(1) Wilson*, N., Gantulga, D., McMaster, N., Knott, K., McCormick, S., Senger, R.S., Schmale, D. (December 2016) DON modification in naturally-contaminated wheat samples using microorganisms isolated from the environment. U.S. Wheat and Barley Scab Initiative (USWBSI). St. Louis, MO (oral presentation)

(2) Dashiell*, S., Wilson*, N., Senger, R.S., Schmale, D., Bohland, C. (November 2016) Mycotoxin education unit for Roanoke Valley Governor’s School. Plant Pathology, Physiology, and Weed Science Mini-Symposium. Blacksburg, VA (poster presentation)

(3) Fisher*, A., Senger, R.S., Bevan, D.R. (November 2016) Constructing a biosynthetic pathway to 3-hydroxy-2-pyrrolidinone. 2016 BI Research Symposium. Blacksburg, VA. (poster presentation)

(4) Gillam, F., Tanniche*, I., Fisher*, A., Senger, R.S., Zhang, C. (July 2016) PCR based cloning: A fast, cheap, and easy way to customize proteins at the DNA level. PREP 2016

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– 29th International Symposium on Preparative and Process Chromatography. Philadelphia, PA (poster presentation)

(5) Yen*, J.Y., Gillaspy, G.E., Senger, R.S. (May 2015) A “genome-scale” algorithmic approach for metabolic engineering of plants. GLBIO/CCBC Great Lakes Bioinformatics and Canadian Computational Biology Conference. Toronto, Canada (oral presentation)

(6) Yen*, J.Y., Gillaspy, G.E., Senger, R.S. (May 2015) A “genome-scale” algorithmic approach for metabolic engineering of plants. GLBIO/CCBC Great Lakes Bioinformatics and Canadian Computational Biology Conference. Toronto, Canada (poster presentation)

(7) Tanniche*, I., Senger, R.S. (April 2016) Improved forms of omega-PCR for DNA plasmid construction and manipulation. American Society for Agricultural and Biological Engineers (ASABE) Southeastern Regional Rally. Blacksburg, VA (poster presentation)

(8) Guimaraes*, P.I., Senger, R.S. (April 2016) Anaerobic digestion and algae cultivation as a platform for high-value chemical production. American Society for Agricultural and Biological Engineers (ASABE) Southeastern Regional Rally. Blacksburg, VA (poster presentation)

(9) Yen*, J.Y., Senger, R.S., Gillaspy, G.E. (March 2016) Genome-scale models for metabolic engineering. Virginia Tech Graduate Student Assembly Symposium. Blacksburg, VA (oral presentation)

(10) Guimaraes*, P.I., Senger, R.S. (March 2016) Anaerobic digestion and algae cultivation as a platform for high-value chemical production. Virginia Tech Graduate Student Assembly Symposium. Blacksburg, VA (poster presentation)

(11) Wilson*, N., Gantulga, D., McMaster, N., Senger, R.S., McCormick, S., Schmale, D. (March 2016) Using enzymes and microorganisms to modify the mycotoxin deoxynivalenol. Virginia Tech Graduate Student Assembly Symposium. Blacksburg, VA (poster presentation)

(12) Tanniche*, I., Senger, R.S. (March 2016) Lambda-PCR and improved omega-PCR strategies for precise plasmid sequence modification. Virginia Tech Graduate Student Assembly Symposium. Blacksburg, VA (poster presentation)

(13) Robertson, J., Senger, R.S. (March 2016) Redefining the management of renal disease through Chemometric FingerprintingTM of urine and dialysate. VT Knowledgeworks Innovation Challenge, Blacksburg, VA (oral presentation)

(14) Guimaraes*, P.I., Senger, R.S. (March 2016) Anaerobic digestion and algae cultivation as a platform for high-value chemical production. Brazilian Graduate Students Conference (BRASCON). Cambridge, MA (poster presentation)

(15) Senger, R.S. (February 2016) Bioenergy research projects in the Metabolic Engineering and Systems Biology Lab at Virginia Tech. US Department of Energy. Washington DC (slide show presentation)

(16) Wilson*, N., Gantulga, D., McMaster, N., Senger, R.S., Schmale, D. (December 2015) Using enzymes and microorganisms to modify the mycotoxin deoxynivalenol. U.S. Wheat and Barley Scab Initiative (USWBSI). St. Louis, MO (poster presentation)

(17) Tanniche*, I., Senger, R.S. (November 2015) Improved forms of omega-PCR for DNA plasmid construction and manipulation. American Institute of Chemical Engineers (AIChE) Annual Meeting. Salt Lake City, UT (poster presentation)

(18) Fisher*, A.K., Bevan, D.R., Senger, R.S. (November 2015) Constructing a biosynthetic pathway to 3-hydroxy-2-pyrrolidinone. American Institute of Chemical Engineers (AIChE) Annual Meeting. Salt Lake City, UT (poster presentation)

(19) Wilson*, N., Gantulga, D. McMaster, N., Senger, R.S., Schmale, D. (November 2015) Using enzymes and microbes to detoxify the mycotoxin deoxynivalenol. Translational Plant Science Mini-Symposium. Blacksburg, VA (poster presentation)

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(20) Serandour, G., He, J., Grove, D., Senger, R.S. (September 2015) Fish waste management. 10th Energy Harvesting Workshop. Blacksburg, VA (oral presentation)

(21) Yen*, J.Y., Gillaspy, G.E., Senger, R.S. (March 2015) Systems metabolic engineering of Arabidopsis for increased cellulose production. American Society for Biochemistry and Molecular Biology (ASBMB) Annual Meeting. Boston, MA (oral presentation)

(22) Yen*, J.Y., Gillaspy, G.E., Senger, R.S. (February 2015) Systems metabolic engineering of Arabidopsis for increased cellulose production. Gordon Research Seminar: Plant Lipids: Structure, Metabolism, and Function. Galveston, TX (poster presentation)

(23) Wilson*, N., Gantulga, D., McMaster, N., Senger, R.S., Schmale, D. (February 2015) Modification of the mycotoxin deoxynivalenol with enzymes and microorganisms. Translational Plant Science Mini-Symposium. Blacksburg, VA (poster presentation)

(24) Wilson*, N., Gantulga, D., McMaster, N., Senger, R.S., Schmale, D. (February 2015) Modification of the mycotoxin deoxynivalenol with enzymes and microorganisms. Plant Pathology, Physiology, and Weed Science Mini-Symposium. Blacksburg, VA (poster presentation)

(25) Freedman*, B.G., Senger, R.S. (November 2014) Combinatorial metabolic engineering with genomic libraries constructed from degenerate oligonucleotide-primed PCR with thermodynamically designed primers. American Institute of Chemical Engineers (AIChE) Annual Meeting. Atlanta, GA (oral presentation)

(26) Nazem-Bokaee*, H., Yen*, J.Y., Senger, R.S. (November 2014) Novel tools in genome-scale metabolic flux modeling to identify metabolic engineering targets and predict microbial phenotypes. American Institute of Chemical Engineers (AIChE) Annual Meeting. Atlanta, GA (oral presentation)

(27) Zu*, T.N.K., Athamneh*, A.I.M, Robertson, J., Senger, R.S. (November 2014) Monitoring the health of ex vivo perfused organs for transplantation using new tools in Raman spectroscopy. American Institute of Chemical Engineers (AIChE) Annual Meeting. Atlanta, GA (oral presentation)

(28) Senger, R.S., Yen*, J.Y., Gillaspy, G.E. (October 2014) Model-guided engineering of plants for production of renewable materials. Institute for Critical Technologies and Applied Science (ICTAS) Program Review. Blacksburg, VA (oral presentation)

(29) Yen*, J.Y., Gillaspy, G., Senger, R.S. (June 2014) A computationally-driven metabolic engineering strategy to increase cellulose in plants. Metabolic Engineering X Conference. Vancouver, British Columbia, Canada (poster presentation)

(30) Senger, R.S. (May 2014) Flux Balance Analysis with Flux Ratios (FBrAtio): A genome-scale modeling methodology to design metabolic engineering strategies. 3rd Annual Constraint Based Reconstruction and Analysis Conference. Wintergreen, VA (oral presentation)

(31) Nazem-Bokaee*, H., Zu*, T.N.K., Collakova, E., Senger, R.S. (May 2014) Reconciling observed and predicted cellular metabolic fluxes using genome-scale modeling, a genetic algorithm, and Raman spectroscopy. 3rd Annual Constraint Based Reconstruction and Analysis Conference. Wintergreen, VA (poster presentation)

(32) Zu*, T.N.K., Athamneh*, A.I.M., Senger, R.S. (April 2014) Phenotypic profiling of bacteria and perfused organs using Raman spectroscopy. Biobased Materials Research Center Graduate Research Symposium. Blacksburg, VA (oral presentation)

(33) Tanniche*, I., Nazem-Bokaee*, H., Senger, R.S. (April 2014) Correlating antisense RNA performance with thermodynamic calculations. Biobased Materials Research Center Graduate Research Symposium. Blacksburg, VA (poster presentation)

(34) Zu*, T.N.K., Athamneh*, A.I.M., Senger, R.S. (March 2014) Raman spectroscopy for near real-time analysis of phenotypic changes in cells, tissues, and organs. American Chemical Society (ACS) National Meeting and Exposition. Dallas, TX (oral presentation)

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(35) Freedman*, B.G., Senger, R.S. (November 2013) Combinatorial metabolic engineering of Clostridium cellulolyticum using clonable genomic library fragments generated from degenerate oligonucleotide primed PCR. American Institute of Chemical Engineers (AIChE) Annual Meeting San Francisco, CA (oral presentation)

(36) Zu*, T.N.K., Athamneh*, A.I.M., Senger, R.S. (November 2013) Raman spectroscopy to monitor the phenotypic responses of bacteria to environmental stresses. American Institute of Chemical Engineers (AIChE) Annual Meeting San Francisco, CA (oral presentation)

(37) Nazem-Bokaee*, H., Senger, R.S. (November 2013) Optimizing the biomass equation of a genome-scale model using simple measurements obtained by Raman spectroscopy. American Institute of Chemical Engineers (AIChE) Annual Meeting San Francisco, CA (oral presentation)

(38) Freedman*, B.G., Senger, R.S. (November 2013) FITSelect: A synthetic gene circuit to guide combinatorial metabolic engineering experiments. American Institute of Chemical Engineers (AIChE) Annual Meeting San Francisco, CA (poster presentation)

(39) Yen*, J.Y., Gillaspy, G.E., Senger, R.S. (August 2013) Systems metabolic engineering of Arabidopsis for increased cellulose production. Virginia Tech Biological Systems Engineering Department Research Symposium. Blacksburg, VA (poster presentation)

(40) Zu*, T.N.K., Athamneh*, A.I.M, Collakova, E., Senger, R.S. (August 2013) Near real-time study of the phenotypic response of E. coli to n-butanol exposure using Raman spectroscopy. Virginia Tech Biological Systems Engineering Department Research Symposium. Blacksburg, VA (poster presentation)

(41) Yen*, J.Y., Gillaspy, G.E., Senger, R.S. (June 2013) Systems metabolic engineering of Arabidopsis for increased cellulose production. Gordon Research Conferences: Plant Metabolic Engineering. Waterville Valley, NH (oral presentation)

(42) Athamneh*, A.I.M., Senger, R.S. (March 2013) Raman spectroscopy for metabolic engineering applications. Institute for Biological Engineers (IBE) International Conference. Durham, NC (oral presentation)

(43) Yen*, J.Y., Nazem-Bokaee*, H., Freedman*, B.G., Athamneh*, A.I., Senger, R.S. (March 2013) Deriving metabolic engineering strategies with flux ratios genome-scale modeling. Institute for Biological Engineers (IBE) International Conference. Durham, NC (oral presentation)

(44) Nazem-Bokaee*, H., Senger, R.S. (March 2013) Host selection for synthetic pathways using a computational systems biology approach to explore biodiversity. Institute for Biological Engineers (IBE) International Conference. Durham, NC (oral presentation)

(45) Tanniche*, I., Nazem-Bokaee*, H., Senger, R.S. (March 2013) Antisense RNA: A metabolic switch for controlling gene expression. Institute for Biological Engineers (IBE) International Conference. Durham, NC (poster presentation)

(46) *Yen, J.Y., Gillaspy, G., Senger, R.S. (February 2012) Metabolic engineering of high cellulose accumulation in plants through genome-scale modeling. 2013 MPS Mini-Symposium. Blacksburg, VA (oral presentation)

(47) Alajlouni, R.A., Athamneh*, A.I., Mayhoub, A.S., Cushman, M., Senger, R.S., Seleem, M.N. (December 2012) Application of Raman spectroscopy in antimicrobial drug discovery research. Research Workers in Animal Diseases Conference. Chicago, IL (poster presentation)

(48) Yen*, J.Y., Nazem-Bokaee*, H., Freedman*, B.G., Athamneh*, A.I., Senger, R.S. (October 2012) Metabolic engineering in silico enabled by manipulating metabolic pathway flux ratios. American Institute of Chemical Engineers (AIChE) Annual Meeting. Pittsburgh, PA (oral presentation)

(49) Athamneh*, A.I., Senger, R.S. (October 2012) Detailed cellular phenotype measurements using peptide-guided surface enhanced Raman scattering (pg-SERS).

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American Institute of Chemical Engineers (AIChE) Annual Meeting. Pittsburgh, PA (oral presentation)

(50) Freedman*, B.G., Senger, R.S. (October 2012) Metabolic production and cloning of error-prone genomic libraries. American Institute of Chemical Engineers (AIChE) Annual Meeting. Pittsburgh, PA (poster presentation)

(51) Yen*, J., Gillaspy, G., Senger, R.S. (August 2012) Metabolic engineering high cellulose accumulation in plants through genome-scale modeling. Virginia Tech Biological Systems Engineering Department Research Symposium. Blacksburg, VA (poster presentation)

(52) Nazem-Bokaee*, H., Yen*, J., Athamneh*, A.I.M., Apte*, A.A., McAnulty*, M.J., Senger, R.S. (August 2012) Host selection for synthetic pathways using a genome-scale model database. Virginia Tech Biological Systems Engineering Department Research Symposium. Blacksburg, VA (poster presentation)

(53) McAnulty*, M.J., Yen*, J., Freedman*, B.G., Senger, R.S. (July 2012) A new approach to modeling gene regulation using flux ratios and the implications for butanol production from clostridia. American Society for Agricultural and Biological Engineers (ASABE) Annual International Meeting. Dallas, TX (oral presentation)

(54) Athamneh*, A.I., Zu*, T.N.K., Senger, R.S. (July 2012) Detailed cellular phenotype measurements using Raman spectroscopy. American Society for Agricultural and Biological Engineers (ASABE) Annual International Meeting. Dallas, TX (oral presentation)

(55) Apte*, A.A., Senger, R.S. (July 2012) SyPath: An algorithm for generating metabolic pathways leading to novel biofuels. American Society for Agricultural and Biological Engineers (ASABE) Annual International Meeting. Dallas, TX (oral presentation)

(56) Zhou*, R., Senger, R.S. (July 2012) FITSelect: An invention to select microbial strains maximizing product formation from a single culture without high-throughput screening. American Society for Agricultural and Biological Engineers (ASABE) Annual International Meeting. Dallas, TX (poster presentation)

(57) McAnulty*, M.J., Yen*, J., Freedman*, B.G., Senger, R.S. (June 2012) Metabolic engineering in silico enabled by genome-scale models with flux ratio constraints. Metabolic Engineering IX Conference. Biarritz, France (poster presentation)

(58) Nazem-Bokaee*, H., Senger, R.S. (May 2012) Host selection using a novel database of standardized genome-scale models. Biobased Materials Research Center Graduate Research Symposium. Blacksburg, VA (oral presentation)

(59) Athamneh*, A.I.M., Senger, R.S. (May 2012) Raman spectroscopy in systems biology and metabolic engineering. Biobased Materials Research Center Graduate Research Symposium. Blacksburg, VA (oral presentation)

(60) Yen*, J.Y., Senger, R.S., Gillaspy, G.E. (April 2012) The utilization of a genome-scale model to explore mutations that increase cellulose in Arabidopsis thaliana. Virginia Tech Molecular Plant Science Mini-Symposium. Blacksburg, VA (poster presentation)

(61) Yen*, J.Y., Senger, R.S., Gillaspy, G.E. (April 2012) The utilization of a genome-scale model to explore mutations that increase cellulose in Arabidopsis thaliana. American Society for Plant Biologists Annual Meeting. Myrtle Beach, SC (oral presentation)

(62) Freedman*, B.G., Senger, R.S. (March 2012) GenoCAD for building synthetic biology parts. American Society for Agricultural and Biological Engineers (ASABE) Southeastern Regional Rally. Blacksburg, VA (oral presentation)

(63) Nazem-Bokaee*, H., Senger, R.S. (March 2012) Mathematics of cell metabolism. American Society for Agricultural and Biological Engineers (ASABE) Southeastern Regional Rally. Blacksburg, VA (oral presentation)

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(64) Apte*, A.A., Senger, R.S. (March 2012) Global sensitivity analysis for metabolic engineering. American Society for Agricultural and Biological Engineers (ASABE) Southeastern Regional Rally. Blacksburg, VA (oral presentation)

(65) Athamneh*, A., Senger, R.S. (March 2012) Measurements of bacteria cell composition using Raman spectroscopy. American Society for Agricultural and Biological Engineers (ASABE) Southeastern Regional Rally. Blacksburg, VA (oral presentation)

(66) Apte*, A.A., Senger, R.S. (March 2012) Generating synthetic pathways for valuable bio-commodities. Biobased Materials Research Center Graduate Research Symposium. Blacksburg, VA (oral presentation)

(67) Senger, R.S. (March 2012) Flux ratio constraints to enable metabolic engineering in silico of clostridial metabolism. Institute of Biological Engineers (IBE) International Conference. Indianapolis, IN (oral presentation)

(68) Senger, R.S. (October 2011) Phenotype prediction through genome-scale modeling and Raman spectroscopy. American Institute of Chemical Engineering (AIChE) Annual Meeting. Minneapolis, MN (oral presentation)

(69) Freedman*, B.G., Senger, R.S. (October 2011) Improving the growth rate of cellulolytic clostridia through genomic library enrichment. American Institute of Chemical Engineering (AIChE) Annual Meeting. Minneapolis, MN (oral presentation)

(70) Apte*, A.A., Senger, R.S. (October 2011) Designing novel cellulase systems through agent based modeling and global sensitivity analysis. American Institute of Chemical Engineering (AIChE) Annual Meeting. Minneapolis, MN (oral presentation)

(71) McAnulty*, M.J., Senger, R.S. (October 2011) Rigorous proton balancing and other new tools to reduce the phenotypic solution space of a genome-scale model. American Institute of Chemical Engineering (AIChE) Annual Meeting. Minneapolis, MN (poster presentation)

(72) Nazem-Bokaee*, H., Yen*, J., Athamneh*, A., Apte*, A.A., McAnulty*, M.J., Senger, R.S. (October 2011) Host selection for synthetic pathways using a genome-scale model database. American Institute of Chemical Engineering (AIChE) Annual Meeting. Minneapolis, MN (poster presentation)

(73) Nazem-Bokaee*, H., Senger, R.S. (October 2011) Genetic regulation of the cellulosome in Clostridium acetobutylicum. American Institute of Chemical Engineering (AIChE) Annual Meeting. Minneapolis, MN (poster presentation)

(74) Apte*, A.A., Senger, R.S. (October 2011) Generating synthetic pathways for valuable bio-commodities. American Institute of Chemical Engineering (AIChE) Annual Meeting. Minneapolis, MN (poster presentation)

(75) Senger, R.S. (August 2011) Genome-scale modeling for the production of value-added bioproducts from microbes. American Society for Agricultural and Biological Engineering (ASABE) International Conference. Louisville, KY (oral presentation)

(76) Lee*, P.W., Senger, R.S. (August 2011) Combinatorial metabolic engineering of Clostridium cellulolyticum to create a host capable of converting cellulose to advanced liquid biofuels. Virginia Tech Summer Undergraduate Research Fellowship (SURF) Symposium. Blacksburg, VA (poster presentation)

(77) Senger, R.S. (June 2011) Engineering phenotypes using genome-scale modeling and Raman spectroscopy. 1st Annual Constraint Based Reconstruction and Analysis Conference. Reykjavik, Iceland (poster presentation)

(78) Senger, R.S. (May 2011) Novel biofuels through synthetic biology. Oak Ridge National Laboratory Biofuels Symposium. Oak Ridge, TN (oral presentation)

(79) Zhou*, R., Senger, R.S. (April 2011) FITSelect: An invention to select microbial strains maximizing product formation from a single culture without high-throughput screening. Virginia Tech ICTAS Bio-Based Materials Center Symposium. Blacksburg, VA (poster presentation)

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(80) McAnulty*, M.J., Senger, R.S. (April 2011) Total proton flux and balancing in genome-scale models: The case for the updated model of Clostridium acetobutylicum ATCC 824. Virginia Tech ICTAS Bio-Based Materials Center Symposium. Blacksburg, VA (oral presentation)

(81) Senger, R.S. (March 2011) Narrowing the solutions of genome-scale modeling through proton efflux, rigorous proton balancing, and optimizing the biomass equation. Institute of Biological Engineers (IBE) International Conference. Atlanta, GA (oral presentation)

(82) Senger, R.S. (November 2010) The genome-scale model biomass constituting equation plays a large role in predicting biofuels production. American Institute of Chemical Engineering (AIChE) Annual Meeting. Salt Lake City, UT (oral presentation)

(83) Freedman*, B.G., Senger, R.S. (November 2010) A combinatorial approach to genetic library enrichment of Clostridium cellulolyticum on lignocellulose. American Institute of Chemical Engineering (AIChE) Annual Meeting. Salt Lake City, UT (poster presentation)

(84) Zhou*, R., Senger, R.S. (November 2010) FITSelect: Genomic library enrichment for product formation without high-throughput screening. American Institute of Chemical Engineering (AIChE) Annual Meeting. Salt Lake City, UT (poster presentation)

(85) Nazem-Bokaee*, H., Wong*, S., Mayo*, C., Senger, R.S. (November 2010) Regulation of the cellulosomal operon in Clostridium acetobutylicum ATCC 824. American Institute of Chemical Engineering (AIChE) Annual Meeting. Salt Lake City, UT (poster presentation)

(86) Nazem-Bokaee*, H., Freedman*, B.G., Senger, R.S. (October 2010) Improving the growth rate of Clostridium cellulolyticum H10 with genomic libraries and genome-scale modeling. Clostridium XI Conference. San Diego, CA (poster presentation)

(87) Senger, R.S. (June 2010) Increased microbial biofuels production through systems biology. American Society of Agricultural and Biological Engineers (ASABE) Annual International Meeting. Pittsburgh, PA (oral presentation)

(88) Freedman*, B.G., Senger, R.S. (June 2010) A combinatorial approach to genetic library enrichment of Clostridium cellulolyticum on cellulose. American Society of Agricultural and Biological Engineers (ASABE) Annual International Meeting. Pittsburgh, PA (poster presentation)

(89) Nazem-Bokaee*, H., Senger, R.S. (June 2010) Determination of the cellulosomal operon regulators and inhibitors in Clostridium acetobutylicum. American Society of Agricultural and Biological Engineers (ASABE) Annual International Meeting. Pittsburgh, PA (poster presentation)

(90) Zhou*, R., Senger, R.S. (June 2010) FITSelect: An invention to select microbial strains maximizing product production from a single culture without high-throughput screening. American Society of Agricultural and Biological Engineers (ASABE) Annual International Meeting. Pittsburgh, PA (poster presentation)

(91) Senger, R.S. (June 2010) Identification of cellulosome repressors in solventogenic clostridia. Metabolic Engineering VIII: Metabolic Engineering for Green Growth. Jeju, South Korea (poster presentation)

(92) Senger, R.S. (November 2009) Evolutionary algorithms in metabolic flux analysis: The case for optimizing bio-butanol production from Clostridium acetobutylicum. American Institute of Chemical Engineers (AIChE) Annual Meeting. Nashville, TN (oral presentation)

(93) Senger, R.S. (August 2009) Modeling the dynamic biomass composition of a cell and the connection to bio-butanol production by Clostridium acetobutylicum. American Chemical Society (ACS) National Meeting and Exposition. Washington, D.C. (oral presentation)

(94) Mayo*, C.C., Senger, R.S. (August 2009) Engineering cellulose utilization by the bio-butanol producer Clostridium acetobutylicum. Virginia Tech Summer Undergraduate Research Fellowship (SURF) Symposium. Blacksburg, VA (poster presentation)

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(95) Senger, R.S. (July 2009) Optimizing the biomass constituting equation of a genome-scale model: The case for the bio-butanol producer Clostridium acetobutylicum. Biochemical Engineering XIV Conference. Burrlington, VT (poster presentation)

(96) Senger, R.S. (November 2008) The dynamic biomass constituting equation for the biofuels producer Clostridium acetobutylicum ATCC 824. American Institute of Chemical Engineers (AIChE) Annual Meeting. Philadelphia, PA (oral presentation)

(97) Senger, R.S., Papoutsakis, E.T. (October 2008) A genome-scale model for Clostridium acetobutylicum. Clostridium X Conference. Wageningnen, The Netherlands (poster presentation)

(98) Senger, R.S., Papoutsakis, E.T. (September 2008) Predicting proton flux with the genome-scale model of Clostridium acetobutylicum: The model organism for butanol production. Metabolic Engineering VII Conference. Puerto Vallarta, Mexico (poster presentation)

(99) Senger, R.S., Papoutsakis, E.T. (August 2008) A genome-scale model for Clostridium acetobutylicum ATCC 824. American Chemical Society (ACS) National Meeting and Exposition. Philadelphia, PA (oral presentation)

(100) Senger, R.S., Karim, M.N. (July 2007) Optimization of fed batch parameters and harvest time of CHO cell cultures for a glycosylated product with multiple mechanisms of inactivation. Biochemical Engineering XV Conference. Quebec City, Canada (poster presentation)

(101) Karra, S., Senger, R.S., Karim, M.N. (November 2006) Model based control of wastewater neutralization. American Institute of Chemical Engineers (AIChE) Annual Meeting. San Francisco, CA (oral presentation)

(102) Karra, S., Senger, R.S., Karim, M.N. (November 2006) Fuzzy steady state decomposition based multi model control of nonlinear processes applied to pH control.American Institute of Chemical Engineers (AIChE) Annual Meeting. San Francisco, CA (poster presentation)

(103) Senger, R.S., Karim, M.N. (March 2005) Variable site-occupancy classification of N-linked glycosylation using artificial neural networks. American Chemical Society (ACS) National Meeting and Exposition. San Diego, CA (oral presentation)

(104) Senger, R.S., Karim, M.N. (March 2004) Neural network-based identification of r-tPA production and glycosylation in CHO cells. Computer Applications in Biotechnology 9 (CAB9) Conference. Nancy, France (oral presentation)

(105) Senger, R.S., Karim, M.N. (March 2004) Data-based prediction of variable site-occupancy of N-linked glycosylation. Computer Applications in Biotechnology 9 (CAB9) Conference. Nancy, France (poster presentation)

(106) Senger, R.S., Karim, M.N. (November 2002) Effect of Shear Stress on Recombinant Tissue-Type Plasminogen Activator (r-tPA) Protein Production and Glycosylation from CHO Cell Culture. American Institute of Chemical Engineers (AIChE) Annual Meeting. Indianapolis, IN (oral presentation)

(107) Senger, R.S., Karim, M.N. (November 2001) Effect of Shear Stress on Recombinant Tissue Plasminogen Activator Production and Glycosylation. American Institute of Chemical Engineers (AIChE) Annual Meeting. Reno, NV (poster presentation)