Running GC. 1.Launch the “TotalChrom” Software 2.Click on “Run” 3.Select “Take Control…” 4.Select “Clarus 580” 5.Click “OK”

Running GC

Running GC1. Launch the

“TotalChrom” Software

2. Click on “Run”3. Select “Take Control…”4. Select “Clarus 580”5. Click “OK”

Running GC

• The GC touch screen panel should show that the GC is in external control.

• Once in external control:• Build Method• Build Sequence

Build Method

Build Method

1. Click on “Method” button

Build Method

2. Select “Create new method”

3. Click on “OK”

Build Method4. Type in a description5. Click “Next”

6. Type in a header text7. Click “Next”

• The Data Acquisition window should look like the following

8. Click “Next”

Build Method

• Autosampler tab should look like the following

• Adjust as you see fit• What is shown is a good

standard 9. Click “Oven/Inlets” Tab

Build Method

10. Adjust the Oven Ramp program as directed.• Make sure to hit enter

on the keyboard after every entry

11. Adjust the “Injector A” temperature. Typically this is set around 225 C.

12. Click on “Carrier” Tab

Build Method

13. Adjust the Carrier tab as directed.• Typical settings are

shown.14. Click on “Detectors” Tab

Build Method

15. Adjust the Detectors tab as directed.• Typical settings are

shown.16. Click on “Next”

Build Method

Click “Next”

Click “Next”

Click “Next”

(No Need to Mess with)

Click “Finish”

Build Method

17. File Save18. Create a unique name19. Click on “Save”20. Close Method window

Build Sequence

Build Sequence

1. Click on “Sequence” button

Build Sequence

2. Select “Create new sequence”3. Click on “OK”

Build Sequence

4. Select the listed options5. Click on “OK”

Build Sequence6. Type in a unique name7. Type in which vial to be used8. Select a method (brings up a

new window)9. Click on “Add+Close” if all vials

are added or click on “Add” to add more vials

Build Sequence

10. File Save11. Create a unique name12. Click on “Save”13. Close Sequence window

Running Sequence

Running Sequence

1. Click on “Setup” button

Running Sequence

1. Select a sequence (new window will open / not shown)

2. Click OK

Running Sequence• The GC will take some

time to upload the sequence information, equilibrate, and do pre-run operation

• You will know that the sequence upload properly because the Instrument-Hands On and Instrument-Modify will no longer be grayed out

• Once the everything is ready the lettering here should be in green.

• I/F will read Ready3. Click on Run4. Start Run