Functional conservation and diversif ication of class E floral homeotic g enes in rice (Oryza sativa) Rongfeng Cui, Jiakun Han, Suzhen Zhao, K unmei Su, Feng Wu, Xiaoqiu Du, Qijiang X u, Kang Chong, Gu¨nter Theißen,* and Zheng Meng* 報報報 : 報報報 N99H0011 報報報報 : 報報報 報報報 報報 報報 :05/08/2011
23
Embed
Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong,
Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa). Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong, Gu¨nter Theißen,* and Zheng Meng*. 報告者 : 劉育羣 N99H0011 專討老師 : 鄭伯智 林宏榮 醫師 日期 : 05/08/2011. - PowerPoint PPT Presentation
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa)
Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong,Gu¨nter Theißen,* and Zheng Meng*
• The MADS-box family transcription factors play key roles in controlling development and signal transduction in eukaryotes.
• Phylogenetic analyses split the MADS-box gene family into two classes, termed type I (SRF-like) and type II (MEF2-like) genes.
MADS-box Gene FamilyMADS-box Gene Family
Messenguy and Dubois (2003)
Alvarez-Buylla et al. (2000)
de Folter S and Angenent GC (2006)
Soltis DE et al., (2007)
New Floral ABCE and Quartet Model
Se – sepal ( 花萼 )
Pe – petal ( 花瓣 )
St – stamen ( 雄蕊 )
Ca – carpel ( 心皮 )
Evolutionary implications of class Evolutionary implications of class E E genesgenes in rice in rice
The class E gene function specifying the identity of lodicules, stamens and carpels may require the direct interaction of OsMADS1 and OsMADS7/8 during spikeletmeristem development.
• Rice transformation• Scanning electron microscopy• In situ hybridization• RT-PCR and real-time PCR expression analysis• Yeast two-hybrid (Y2H) analyses• Coimmunoprecipitation (Co-IP) analyses of the ric
e SEP-like proteins in Arabidopsis mesophyll protoplasts
Experimental Procedures
Results
Molecular characterization of RNAi transgenic plants for rice SEP-ike genes
Phenotypes of wild-type and OsM7/8-RNAi spikelets
WT WTOsM7/8 -RNAi
OsM7/8-RNAi
OsM7/8 -RNAi
Scanning electron microscopy of epidermal cells expression patterns detailed structure
OsM7/8-RNAi
WT
OsM7/8 -RNAi
藉由這樣的實驗作者是要看小穗在 OsM7/8-RNAi 和 WT 當中,表皮細胞細部構造的
表現情形
Phenotypes of the OsM1/5/7/8-RNAi inflorescences and spikelets
WTOsM1/5/7/8-RNAi OsM1/5/7/8-RNAi
6 9
OsM1/5/7/8-RNAi
Under the Scanning electron microscopy the different developmental stages, formation
of inflorescence meristem changes
藉由這樣的實驗作者是要看小穗在 OsM1/5/7/8-RNAi 和在花序中產生變化所表現的
情形
Characteristics of the florets in transgenic lines
lodicule (漿片 )
stamen (雄蕊 ) carpel (心皮 )palea (內穎 )
lemma (外穎 )
此表格作者所要呈現的是轉殖株在花部位的特性
Expression patterns of OsMADS7 and OsMADS8 during inflorescence and spikelet development as revealed by in situ
hybridization
OsMADS7
OsMADS8 OsMADS7 OsMADS8
pbm代表主要分支
sbm代表次要分支smc代表分生組織細胞 ;tsn代表末端分生組織 ;lsm代
表橫向分生組織
OsMADS7
OsMADS8
OsMADS7 OsMADS8
Meristem at different times for each organ, in part on the show are different
藉由這樣的實驗作者是要看 OsMADS7.OsMADS8 在花序與小穗中分生組織表現的情形
fm代表分生組織
Yeast two-hybrid assays. Interaction patterns of the rice SEP-like proteins including OsMADS1, OsMADS5, OsMADS7 and
OsMADS8 are shown
藉由這樣的實驗作者證實了 OsMADS1.OsMADS7.OsMADS8 蛋白彼此之間的結合
Interaction analyses of four rice class E proteins in vivo
藉由這樣的實驗作者證實了水稻中 E class 蛋白彼此之間的結合能力
conclusion
• Silencing of both OsMADS7 and OsMADS8 causes severe morphological alterations of floral organs.
• Simultaneous silencing of OsMADS1, OsMADS5, OsMADS7 and OsMADS8 transforms all floral organs into leaf-like structures.
• OsMADS1, OsMADS7 and OsMADS8 can cross-interact to each other while OsMADS5 can not.