RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Page 1 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=1' data-page='1' class='cut-thumb lazyload' alt='Page 1: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/1.jpg' width='140' height='200' /> RNA Extrac+on: Trizol

Page 2 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=2' data-page='2' class='cut-thumb lazyload' alt='Page 2: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/2.jpg' width='140' height='200' /> RNA Extrac+on: Trizol

Page 3 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=3' data-page='3' class='cut-thumb lazyload' alt='Page 3: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/3.jpg' width='140' height='200' /> RNA extrac+on from cells, +ssues, organs

Page 4 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=4' data-page='4' class='cut-thumb lazyload' alt='Page 4: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/4.jpg' width='140' height='200' /> Mammalian +ssues (DEPC treated)

Page 5 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=5' data-page='5' class='cut-thumb lazyload' alt='Page 5: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/5.jpg' width='140' height='200' /> RNA Extrac+on

Page 6 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=6' data-page='6' class='cut-thumb lazyload' alt='Page 6: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/6.jpg' width='140' height='200' /> RNA Analysis

OD260/OD280: 1.9-‐2.1 GOOD 2.2 RNA degreda+on OD260/OD230: <2.0 Trizol contamina+on

Page 7 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=7' data-page='7' class='cut-thumb lazyload' alt='Page 7: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/7.jpg' width='140' height='200' /> RNA Electrophoresis

Page 8 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=8' data-page='8' class='cut-thumb lazyload' alt='Page 8: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/8.jpg' width='140' height='200' /> mRNA prepara+on

Page 9 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=9' data-page='9' class='cut-thumb lazyload' alt='Page 9: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/9.jpg' width='140' height='200' /> Page 10 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=10' data-page='10' class='cut-thumb lazyload' alt='Page 10: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/10.jpg' width='140' height='200' /> Reverse Transcrip+on PCR

Page 11 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=11' data-page='11' class='cut-thumb lazyload' alt='Page 11: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/11.jpg' width='140' height='200' /> Hybridiza+on

Page 12 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=12' data-page='12' class='cut-thumb lazyload' alt='Page 12: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/12.jpg' width='140' height='200' /> Page 13 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=13' data-page='13' class='cut-thumb lazyload' alt='Page 13: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/13.jpg' width='140' height='200' /> Northern Blot

Page 14 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=14' data-page='14' class='cut-thumb lazyload' alt='Page 14: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/14.jpg' width='140' height='200' /> Northern Blot

Page 15 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=15' data-page='15' class='cut-thumb lazyload' alt='Page 15: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/15.jpg' width='140' height='200' /> RNA in situ hybridiza+on

Page 16 <img data-url='document/rnaextracontrizol-23s-rrna-16s-rrna-28-s-18-s-sim-agarose-gel-electrophoresis.html?page=16' data-page='16' class='cut-thumb lazyload' alt='Page 16: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA' loading='lazy' src='data:image/gif;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAABCAYAAAAfFcSJAAAADUlEQVR42mNk+P+/HgAFhAJ/wlseKgAAAABJRU5ErkJggg==' data-src='https://reader039.cupdf.com/reader039/viewer/2022031212/5bd3f21709d3f2b5578b764c/html5/thumbnails/16.jpg' width='140' height='200' /> RNA in situ hybridiza+on

RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

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