Rice et al, Optics Lett, submitte d. 2800 2900 3000 W avenum bers(cm -1 ) A bsorption (au) N anoIR FTIR 4 5 6 7 8 9 0 100 200 300 400 LateralPosition (m m) Height(nm ) 160 200 240 280 Opto-acou (m V) A FM N anoIR A FM NanoIR NanoIR N d:YAG PPLN Q uadrant detector AFM laser cantilever sample Elliptical m irror
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Rice et al, Optics Lett, submitted. NanoIR. Future NanoIR Requirements Applications - Heterogeneous systems - Bacteria, viruses, cells Future developments.
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Rice et al, Optics Lett, submitted.
2800 2900 3000Wavenumbers (cm-1)
Abs
orpt
ion
(au)
NanoIR
FTIR
4 5 6 7 8 90
100
200
300
400
Lateral Position (mm)
Hei
ght
(nm
)
160
200
240
280 Opto-acou
(mV
)
AFM
NanoIR
NanoIR
AFM NanoIR
AFM
NanoIR
Nd:YAGPPLN
Quadrant detector
AFM laser
cantilever
sample
Elliptical mirror
Future
• NanoIR Requirements
• Applications - Heterogeneous systems - Bacteria, viruses, cells
• Future developments
NanoIR
Coli bacteria & phage virus
CLIO (2007)Ultramicroscopy, 107, 1194, (2007)
Heavily infected
AFM Ramansingle S.
epidermidis cells
Uninfected
Experimental equipment
Nano flouresence
E
h
E
h
APL, 86, 213103 (2005)
Science, 316, 1153 (2007)
BIO systems
500 nm Science, 312,
1051 (2006)
The Drosophila coiled-coil domainprotein Bruchpilot (BRP)
Future directions
• Requirements - Enables classical microscopy
• Applications - Cells, nanoarchitectures (neurons) - Hybrid systems