Rev 4/28/06. SPIFE 3000 11 analytes, with multiple gel sizes for every workload Typical throughput – up to 14 SPE and 12 IFE gels in 8 hours Used in hundreds.

Rev 4/28/06

SPIFE 3000SPIFE 3000

• 11 analytes, with multiple gel sizes for every workload

• Typical throughput – up to 14 SPE and 12 IFE gels in 8 hours

• Used in hundredsof medical centers and reference laboratories worldwide

• Semi-automated electrophoresis system

• Automated sample application system

• Automated reagent application for enzymatic assays

SPIFE 3000SPIFE 3000

• Temperature-controlled electrophoresis, incubation and drying

• Automated staining and drying of visible analytes

SPIFE 3000SPIFE 3000

• Positive ID of sample tray and gel

SPIFE AssaysSPIFE Assays• Cholesterol Profile• Serum Proteins• Split-Beta SPE• Urine Proteins• Immunofixation• Urine Immunofixation• Hi Resolution Proteins• Alkaline Hemoglobins• Acid Hemoglobins• CK & LD Isoenzymes• Alk Phosphatase Isoenzymes• CSF IgG IEF• Lipoproteins

SPIFE IFESPIFE IFE

• Built-in QC wells

• Up to 210 profiles in 8 hours

• Antisera affinity & avidity balanced for highest sensitivity with least background

• 1, 2, 3, 6, 9 and 15 profile gels to handle a wide range of needs

• Acid violet stain enhances visualization

SPIFE IFE ProcedureSPIFE IFE Procedure

• Dilute automatically using the Auto Sample Handler or manually

• Load patients samples and applicator blades

• Set gel and electrodes in place in electrophoresis chamber

SPIFE IFE ProcedureSPIFE IFE Procedure

• SPIFE applies samples, allows for absorption, and begins electrophoresis

• Blot surface of the gel and begin electrophoresis

• After electrophoresis, remove buffer ridges from the gel

SPIFE IFE ProcedureSPIFE IFE Procedure

• Set Rigid Antisera Template in place

• Fast and efficient antisera application

• Pipette color-coded antisera for an entire profile at once

SPIFE IFE ProcedureSPIFE IFE Procedure

• Absorb excess antisera from channels, preventing cross-contamination

• Blot gel surface, using antisera template as press and allow SPIFE to incubate and dry the gel

SPIFE IFE ProcedureSPIFE IFE Procedure

• Move gel to SPIFE’s staining unit for automatic wash, stain, destain and dry

• Finished!All SPIFE IFE gels include integral control wells for optional use of ImmunoFix Controls to verify antisera reactivity

• 108 slides

• Basics of protein structure

• Disease states associated with protein abnormalities

• Suggested protocol for evaluating monoclonals

• When to run, how to run, and what to look for

• Patterns to interpret from simple to complex

• Covers serum and urine

New Educational CDProtein Electrophoresis and IFE

An Interpretive Guide

Auto Sample Handler• Provides positive

sample ID from tube to tray to gel

• Primary tube sampling with liquid level sensing

• Bar code reader with LIS interface

Auto Sample HandlerAuto Sample Handler• Automatically performs

dilutions for IFE

• Prepares sample trays, ready for SPIFE

• Interfaces to QuickScan 2000 for workload listing

QuickScan 2000

• Provide IFE and SPE results on a single report

• Patterns can be viewed and edited over the LAN

• 10,000 gel archive with easy retrieval

• Scan anything large or small in seconds:10-sample to 100-samplegels and L/S plates

QuickScan 2000QuickScan 2000

• Customized interpretive analysis and decision limits for fast reporting

• Simple to use – no slits or wavelengths to select

• Multi-pattern display color-coded for in/out of range and reviewed results

• Build your own decision limits and interpretive comments library

• Comments automatically added for “normals” and coded for quick retrieval

QuickScan 2000QuickScan 2000

• Choose quantitation of split beta SPE as one or two fractions

• Report formats can be totally customized

• Profile up to 100 scans

QuickScan 2000QuickScan 2000

SPIFE SystemSPIFE System

Contact your local Helena technical representative for more information or to arrange an in-lab

demonstration of the SPIFE System.

1-800-231-5663