1 Respiratory viral coinfections identified by a 10-plex real time polymerase chain reaction assay in patients hospitalised with Severe Acute Respiratory Illness—South Africa, 2009- 2010 Marthi A Pretorius 1 ;Shabir A Madhi 1,2 ;Cheryl Cohen 1 ; Dhamari Naidoo 1 ;Michelle Groome 2 ; Jocelyn Moyes 1 ; Amelia Buys 1 ; Sibongile Walaza 1 ;Halima Dawood 3 ; Meera Chhagan 3,5 ; Sumayya Haffjee 4 ;Kathleen Kahn 6 ; Adrian Puren 1 ; Marietjie Venter 1, 7 * Authors affiliations: 1) National Institute for Communicable Diseases of the National Health Laboratory Service (NHLS), Johannesburg, South Africa. 2) Department of Science and Technology/National Research Foundation: Vaccine-Preventable Diseases, South Africa. 3) Pietermaritzburg Metropolitan Hospital Complex, Edendale KwaZulu Natal, South Africa. 4) Pietermaritzburg Metropolitan Hospital/NHLS Complex, Edendale KwaZulu Natal, South Africa 5) Maternal and Child Health Unit, University of KwaZulu-Natal, South Africa. 6) MRC/Wits Rural Public Health and Health Transitions Research Unit (Agincourt), School of Public Health, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg. 7) Department Medical Virology, University of Pretoria, Pretoria South Africa Contact details Marthi A Pretorius 1: [email protected]Dhamari Naidoo 1 : [email protected]Shabir A Madhi 1,2 : [email protected]Michelle Groome 2 : [email protected]Amelia Buys 1 : [email protected]Jocelyn Moyes 1 : [email protected]Sibongile Walaza 1 : [email protected]
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Respiratory viral coinfections identified by a 10-plex real time polymerase chain reactionassay in patients hospitalised with Severe Acute Respiratory Illness—South Africa, 2009-
2010
Marthi A Pretorius1;Shabir A Madhi1,2;Cheryl Cohen1; Dhamari Naidoo1;Michelle Groome2;
Background: Data about respiratory co-infections of Influenza A H1N1 during the pandemic in
Africa are limited. We used an existing surveillance programme for severe acute respiratory
illness (SARI) to evaluate a new multiplex real-time polymerase chain reaction assay and
investigate the role of influenza and other respiratory viruses in pneumonia hospitalisations
during and after the influenza pandemic in South Africa.
Method: The multiplex assay was developed to detect 10 respiratory viruses including Influenza
(INF) A and B, Parainfluenza (PIV1-3), Respiratory Syncytial Virus (RSV), Enterovirus (EV),
human metapneumovirus (hMPV), Adenovirus (AdV) and Rhinovirus (RV), followed by influenza
subtyping. Nasopharyngeal and oropharyngeal specimens were collected from patients
hospitalized with pneumonia at six hospitals during 2009–2010.
Results: Validation against external quality controls confirmed the high sensitivity (91%) and
specificity (100%) and user-friendliness when compared to other PCR technologies. Of 8173
patients, 40% had single-infections, 17% co-infections and 43% remained negative. The most
common viruses were: RV (25%), RSV (14%), AdV (13%), Influenza A (5%). Influenza, RSV,
PIV3 and hMPV showed seasonal patterns.
Conclusion: The data provide a better understanding of the viral aetiology of hospitalized
cases of pneumonia and demonstrate the usefulness of this multiplex assay in respiratory
disease surveillance in South Africa.
4
Introduction
Pneumonia is a major cause of morbidity and mortality in children worldwide and causes 18% of
all deaths in children less than 5 years of age [1]. Viral infections have been shown to play a
major role in acute respiratory infections in the developed world, but apart from a few papers on
specific viruses such as influenza and respiratory syncytial virus (RSV) in selected regions, data
remain limited from sub-Saharan Africa [2-4]. In April 2009, Influenza A (H1N1) pdm09
((H1N1)pdm09) emerged as a new pathogen. South Africa reported 12640 cases and 93 deaths
during the first wave from June-October 2009, the most of any country in Africa [5].
Respiratory viruses traditionally associated with acute respiratory tract infection include
influenza (INF) A and B; respiratory syncytial virus (RSV); parainfluenza virus (PIV) types 1, 2
and 3; adenovirus (AdV); enterovirus (EV); human metapneumovirus (hMPV) and rhinovirus
(RV) [6, 7]. While a few studies have determined the frequency of respiratory viruses in patients
with acute lower respiratory tract illness in Africa, [8-11] these studies have mainly been limited
to single sites and a limited number of viruses, and little has been reported on viral co-
infections. Few data are available about the contribution of other respiratory viruses to
respiratory tract infections during the pandemic or their role in (H1N1)pdm09 infections in Africa,
and limited data is available from elsewhere [12, 13].
Comparative studies have shown that the detection of respiratory viruses using real-time
reverse transcriptase polymerase chain reaction (rRT-PCR) assays is substantially more
sensitive than using conventional methods such as viral culture and immunofluorescence
assays (IFA) [14-16]. Furthermore, compared to conventional PCR and other real-time
methods, multiplex rRT-PCR has a significant advantage as it permits simultaneous
amplification of several viruses in a single reaction [4, 15, 16]. This facilitates cost-effective
diagnosis, enabling the detection of multiple viruses in a single clinical specimen.
5
As part of a severe acute respiratory infection (SARI) surveillance programme which
commenced in February 2009 in South Africa, we developed a two-step real-time multiplex
reverse transcriptase PCR (rRT-PCR) assay that could detect ten different viruses (Influenza A
and B, RSV, EV, hMPV, AdV, RV, PIV 1, 2 and 3) in order to investigate the role of the most
common viral agents as aetiological agents in patients hospitalised with SARI in South Africa.
Materials and Methods
Setting
Specimens used in this study were obtained through routine surveillance for hospitalized SARI
in six government hospitals around the country, including Chris Hani Baragwanath (2009-2010),
an urban hospital in Gauteng province; Edendale (2009-2010), a semi-urban hospital in
KwaZulu-Natal province; Matikwana and Mapulaneng (2009-2010), two rural hospitals in the
Bushbuckridge district in Mpumalanga province; and Tshepong and Klerksdorp hospital
complex (2010), semi-urban hospitals in the North-West province (Figure 1). Nasopharyngeal
aspirates were collected from children <5 years old and nasopharyngeal as well as oral
pharyngeal swabs were collected from patients >5 years old. Specimens were sent to the
Respiratory Virus Unit (RVU) at the National Institute for Communicable Diseases (NICD) in
Johannesburg within 72 hours of collection for processing and storage at -70°C.
Case Definition
We defined a case of SARI according to a previously suggested WHO case definition [17] for all
children and adults ≥5 years old, hospitalized with onset of illness within 7 days of admission.
We defined SARI in children 2 days through 2 months old as physician-diagnosed sepsis or
lower respiratory tract infection (LRTI), and we defined SARI in children 3 months through 5
years old as physician-diagnosed acute LRTI. Surveillance officers administered a
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Figure 1: Geographical Map of South Africa indicating the locations of the SARI
surveillance sites.
questionnaire with basic demographic and clinical information and examined medical records to
collect data on admitting diagnoses. Specimens were collected on the day of admission.
Validation and Optimization of Real-Time rRT-PCRMultiplex
A real-time multiplex PCR assay detecting ten different viruses (Influenza A and B, RSV, EV,
hMPV, AdV, RV, PIV 1, 2 and 3) was established as two-step rRT-PCR with 5 separate
reactions (Table 1). The assay was validated using conserved regions of the target viruses, to
7
Table 1: Primers and probes used in multiplex rRT-PCR run in five PCR mixtures as
indicated.
Target Gene Forward
Oligonucleotide sequence (5’- 3’) Reverse
Probe
REPORTER PCR GROUP Published
Parainfluenza
PIV 1HN gene
GTT GTC AAT GTC TTA ATT CGT ATC AAT AAT T
GTA GCC TMC CTT CGG CAC CTA A
TAG GCC AAA GAT TGT TGT CGA GAC TAT TCC AA Cy 5-BBQ
A§
[26]
PIV 2 HN gene
GCA TTT CCA ATC TTC AGG ACT ATG A
ACC TCC TGG TAT AGC AGT GAC TGA AC
CCA TTT ACC TAA GTG ATG GAA TCA ATC GCA AA FAM-BHQ1
[26]
PIV 3 HN gene
AGT CAT GTT CTC TAG CAC TCC TAA ATA CA
ATT GAG CCA TCA TAA TTG ACA ATA TCA A
AAC TCC CAA AGT TGA TGA AAG ATC AGA TTA TGC A Red 610-BHQ2
[26]
Respiratory
Syncytial virus
(RSV)
Matrix
protein
GCA AAT ATG GAA ACA TAC GTG AAC A
GCA CCC ATA TTG TWA GTG ATG CA
CTT CAC GAA GGC TCC ACA TAC ACA GCW G FAM-BHQ1B¥
[7]
Influenza B
(INF B)HA
AAA TAC GGT GGA TTA AAT AAA AGC AA
CCA GCA ATA GCT CCG AAG AAA
CAC CCA TAT TGG GCA ATT TCC TAT GGC Red 610-BHQ2
[27]
Enteroviruses (EV) 5’ UTR
TCC TCC GGC CCC TGA
RAT TGT CAC CAT AAG CAG CCA
CGG AAC CGA CTA CTT TGG GTG WCC GT Cy 5-BBQC¥
[28]
human Meta
pneumovirus
(hMPV)
N
protein
GAA GAR ATA GAC AAA GAR GCA AG
TCC CAC TTC TAT KGT TGA TGC TAG
TCA GCA CCA GAC ACA CC Red 610-LNA
[6]
Adenoviruses
(AdV)Hexon
GCC ACG GTG GGG TTT CTA AAC TT
GCC CCA GTG GTC TTA CAT GCA CAT C
TGC ACC AGA CCC GGG CTC AGG TAC TCC GA Red 610-BHQ2D¥
[29]
Influenza A
(INF A)M1
GAC CRA TCC TGT CAC CTC TGA C
AGG GCA TTY TGG ACA AAK CGT CTA
TGC AGT CCT CGC TCA CTG GGC ACG FAM-BHQ1
*[18]
Rhinoviruses (RV) 5 ‘ UTR
GGT GTG AAG AGC CSC RTG TGC T
GGT GTG AAG ACT CGC ATG TGC T
GGG TGY GAA GAG YCT ANT GTG CT
GGA CAC CCA AAG TAG TYG GTY C
CCG GCC CTG AAT GYG GCT AAY C FAM-BHQ1E¥
[7]
Human sapiens
ribonuclease
RNP (IQC)
30kDa
subunit
(RPP30)
AGA TTT GGA CCT GCG AGC G
GAG CGG CTG TCT CCA CAA GT
TTC TGA CCT GAA GGC TCT GCG CG Cyan 500-BHQ1
*[18]
* Primers and Probes were obtained from the CDC co-operative agreement after the first case of Pandemic H1N1was detected.¥Multiplex was combined from pre-existing published primer and probe sets¥Existing published multiplex
8
minimize the effect of genetic changes within each of the viruses. External quality control
panels which included isolates of PIV 1, 2, 3 and 4; RSV A and B; EV; hMPV I and II; AdV; RV;
and INF A and B viruses and specimens for the bacterial species Chlmaydophila pneumoniae,
Legionella pneumophila and Mycoplasma pneumoniae from Quality Control for Molecular
Diagnostics (QCMD, Glasgow, Scotland) were used to optimize and validate the multiplex
assays. Optimal primer annealing temperatures and primer and probe concentrations were
calculated by experimentation. The QCMD panels were used to test all primers and probes for
possible competitive interactions. The cross reactivity of the assay was assessed in triplicate, to
ensure repeatability, reproducibility, sensitivity and specificity. TaqMan technology was selected
for the multiplex rRT-PCR assay to ensure adaptability to different real-time platforms.
Nucleic acid extraction
The MagNA Pure LC Total Nucleic acid Kit (Roche Diagnostics, Mannheim Germany) was used
according to manufacturer’s instructions using 200 µl sample and a final elution volume of 50 µl;
excess extracted nucleic acids were stored at – 70°C. A negative and positive biological control
was used in each extraction.
Primer and Probe multiplexing
Primers and probes for 10 respiratory viruses (PIV 1, 2 and 3; RSV; EV; hMPV; AdV; RV and
INF A and B viruses) were identified for the qualitative studies. All primers and probes (Table 1)
were optimized in different combinations for this assay. DNAMAN was used (Lynnon
Corporation, Québec, Canada) to ensure primer complementary and primer dimers did not exist
between different PCR groups and to select primer set candidates per multiplex. We used the
influenza A primers recommended by the WHO Collaborating Center for Influenza, Centers for
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Disease Control and Prevention (CDC), USA for the universal detection of Influenza A strains,
which were updated to include the (H1N1)pdm09 [18].
Real-Time RT-PCR
cDNA was synthesized using the Transcriptor 1stStrand cDNA Kit (Roche Diagnostics,
Mannheim Germany), according to manufacturer’s instructions. Qualitative real-time
polymerase chain reactions (PCR) using the LightCycler® 480 Probes Master kit (Roche
Diagnostics, Mannheim Germany) and the LightCycler® 480 System (Roche Diagnostics,
Mannheim Germany) were performed. Each real-time PCR reaction contained 15 µl of 2X
Master Mix, 1 µM of each primer and 0.5 µM of each probe and 10 µl of cDNA reaction mixture
as template for a final volume of 30 µl. PCR cycles was initiated at 95°C for 15 minutes to
activate Taq DNA polymerase enzyme, followed by 45 cycles of 94°C for 15 seconds, 60°C for
20 seconds and 72°C for 10 seconds. Specimens were considered positive when the Ct value
was equal or above the Ct value of the Lower limit of detection of the corresponding virus, which
ranged between Ct=36 to Ct=40. The influenza positive specimens were subtyped using the
CDC Real-time RTPCR (rRTPCR) Protocol for Detection and Characterization of Influenza,
which was distributed to National Influenza Centres under a Material Transfer Agreement [18].
Statistical Analysis
We analysed the positive cases and seasonal patterns of the respiratory viruses included in the
multiplex. Results were analysed according to virus proportion per month and subject age-
group. The Chi-square test and Fischer’s exact test were used for univariate analysis, P-values
<0.05 were considered to be statistically significant. Analysis was performed using STATA 11,
(Stata Corporation, Texas USA). Data for Kappa and Bland-Altman analysis were analysed
10
using Analyse-it® Method Evaluation Edition add-in software for Microsoft Excel 2007 (Analyse-
it Software Ltd., Leeds, UK).
Ethical considerations
The protocol was reviewed and approved by the University of the Witwatersrand Human
Research Ethics Committee (HREC) and the University of KwaZulu Natal Human Biomedical
Research Ethics Committee (BREC) protocol number M081042 and BF157/08, respectively.
Results
Validation and Optimization of the Real-Time RT-PCR (rRT-PCR).
Using the external quality control panels provided by QCMD as the gold standard, the multiplex
rRT-PCR assay had a high overall accuracy (98%) (i.e. the degree of closeness of measured or
calculated quantity to its actual (true) value), negative predicative value (97%), positive
predicative value (100%), sensitivity (91%) and specificity (100%) [19]. The rRT-PCR assay was
compared to Immunofluorescence assays (IFA) and the rRT-PCR assay was more sensitive in
all cases (data not shown). Use of QCMD panels established reproducibility, repeatability, as
well as lower detection limits ranging from Ct values of 36 to 40 depending on the different
viruses. The coefficient of variation calculated from the QCMD panels for the 5 reactions ranged
from 0.2% to 0.7%.
Study group demographics
From February 2009 up to December 2010, we collected and tested specimens from 8173 SARI
patients. The median age of patients was 3 years (range 0-99 years). Half of patients (3974
(51.1%) were male. The largest age group was from children <1 year of age (3157 (38.6%), and
6098 (74.7%) were from patients admitted to Chris Hani Baragwanath Hospital in Soweto.
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Application of the rRT-PCR for screening of surveillance specimens from patients with SARI
Of the 8173 patients tested with the rRT-PCR, 3240 (39.6%) had single infections, 1426
(17.4%) had co-infections with two or more viruses, and 3507 (42.9%) were negative for
pathogens included in this assay. The most common respiratory viruses identified were RV
The Chi-square test and Fischer’s exact test were used for univariate analysis and Odds ratio calculated for eachof the age group. Analysis was performed using STATA 11, (Stata Corporation, Texas USA). P-values <0.05 wereconsidered to be statistically significant.
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comprised of H3N2 (82, 1.8%) and (H1N1)pdm09 (44, 0.9%). During 2009 a total of 14/494
(2.8%) Influenza A specimens could not be subtyped due to too low concentrations. In the
univariate analysis patients infected with (H1N1)pdm09 (OR=2.28, p=0.001), were more likely
between 5-24 years, while patients infected with H3N2 (OR=1.75, p=0.001), were between 2-4
year old, however the patients infected with Influenza B (OR=2.09, p=0.001) were more likely
between 25-44 years (Table 3). In addition no difference was observed between the two years
with regards to the distribution and proportion of each of the other respiratory viruses.
The highest overall virus detection rate was in the 2-4 year old age group, where 833 (83.9%) of
992 specimens were positive for at least one virus, and lowest in persons ≥65 years old, where
55 (24.2%) of 227 specimens were positive for at least one virus. Compared to 2-4 year olds,
the positivity rate for other age groups was significantly less [0-1 years (76.5% positive), 5-24
years (51.0% positive), 25-44 years (33.8% positive), and 45-64 years (28.7% positive;
p<0.001)] for each compared with 2-4 year olds. In the 0-1 year old age group the most
common virus was RV (985/3157 (31.2%)) followed by RSV, (845/3157 (26.7%)). In contrast, in
other age groups, the more common pathogens were RV, and AdV (Table 3).
Respiratory viral co-infections
Among the 1426 patients with co-infections (Table 2, matrix), RV was detected most frequently
[860 (60.3%)] followed by AdV in 719 (50.4%) and RSV in 578 (40.5%). Of the 51 patients with
(H1N1)pdm09 co-infections, RV was detected in 19 (37.3%), followed by PIV-3 in 10 (19.6%).
There were no co-infections with Influenza A (H1N1)pdm09 and H3N2 viruses. However there
was 1 co-infection with Influenza B and H3N2 (Table 2). Of the 94 patients with Influenza A
H3N2 co-infections, RV was detected in 38 (40.4%) followed by RSV in 19 (20.2%) and PIV 3 in
6 (6.4%).
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Of the 1426 co-infections, the following combinations of viruses were detected most frequently
in the same specimen: 356 cases of RV and AdV, 320 cases of RV and RSV, 212 cases of RSV
and AdV and 142 cases of AdV and EV (Table 2, matrix).
Seasonality
Seasonal patterns were visible for RSV, Influenza A and B, EV, hMPV and PIV3 in both 2009
and 2010. RSV occurred from February to June before the influenza season, which typically
falls between May to September [20]. EV was detected throughout the year with peak activity
between February and April and again between November and December. Peak activity for
hMPV was observed between July and August and for PIV3 between September and
November. Adenovirus and RV were detected throughout the two years without seasonal
variability. PIV 1 and PIV 2 were detected sporadically throughout the two years (Figure 2).
The seasonal patterns of both Influenza A and B viruses during 2009 and 2010 were different.
In 2009, Influenza A H3N2 and (H1N1)pdm09 occurred as two waves peaking between May to
July and July to October, respectively, while Influenza B appeared briefly in August. During
2010 Influenza B season predominated from June to November, H3N2 circulated from June to
September and (H1N1)pdm09 circulated between the last week of July and October.
Discussion
The validated assay was implemented for routine surveillance prior to the pandemic and
enabled us to investigate the contribution of other respiratory viruses to SARI during the first two
pandemic seasons. The assay also helped define the distribution and seasonality of these
15
Figure 2: Distribution of Respiratory Viruses detected during 2009 and 2010, showing
specific seasonal trends and peak activity. (Please note that the scales on the y-axis
differ for each graph.)
16
respiratory viruses in South Africa and the role of viral co-infections in hospitalized patients
infected with (H1N1)pdm09 in South Africa during the 2009 and 2010 seasons. Using the
validated rRT-PCR multiplex assay, viral agents were detected in 57% of cases identified
through South Africa’s SARI surveillance network, which is consistent with other studies using
rRT-PCR multiplex assays for the detection of respiratory viruses [2, 4, 16]. Validation of the
rRT-PCR multiplex assay suggested that it is as specific but more sensitive than IFA. The assay
does not give any false positive results while the lower detection limit determined may give false
negatives with specimens with very low viral load.
The majority of patients enrolled were infants less than 1 year of age, and the most commonly
identified pathogens within this group were RSV and RV. While RV was detected throughout the
year, RSV was detected in a distinct seasonal pattern with the peak months of detection
occurring from February to June. Seasonal peaks were also identified for PIV3, hMPV and
enterovirus. Year-round detection of Rhinovirus and Adenovirus make these two viruses the
more likely to co-infect with all other viruses.
Although rhinovirus was the most commonly identified pathogen in this study, further studies are
needed to determine how much rhinovirus contributes to disease severity [4, 9, 21]. In a study
conducted two years prior to the 2009 pandemic in hospitals situated in South Africa, RSV was
detected in a much higher rate in symptomatic infants with severe disease than in asymptomatic
infants attending an immunization clinic in the same region[16]. Nevertheless, in a study
conducted during 2006 and 2007 in South Africa, RV was present in 18% of asymptomatic
children and in >30% of children hospitalized with SARI, which suggests a possible role in
disease severity [4].
Although there are growing concerns for the potential of (H1N1)pdm09 reassorting with existing
human influenza viruses giving rise to a highly transmissible or pathogenic virus [22], no mixed
infections were detected with either subtypes in patients with SARI in this study. The Influenza
17
subtypes had co-circulated for overlapping periods both in 2009 and 2010, but peak months of
detection was distinct for H3N2 and (H1N1)pdm09 in both years, while Influenza B was
detected from June to November, overlapping with both H3N2 and pandemic H1N1 peaks. No
seasonal H1N1 was observed during the 2009 and 2010 influenza season. In the present study
we found pandemic cases mostly in older children and young adults, which is similar to
surveillance reports of the 2009 pandemic in other parts of the world, which have shown that up
to 57% of cases occurred among people between 5–24 years of age with a detection rate of
5.1% [23].
Our study has some limitations. First, rRT-PCR assays are more sensitive for detecting
respiratory viruses compared to viral culture, and with the increased detection of mixed viral
infections, the clinical interpretation of positive PCR results have become more challenging.
Although the viral nucleic acids detected here does not necessarily indicate the presence of
viable virus, several studies have documented few persistent or recurrent PCR-positive
respiratory specimens in patients after acute illness has resolved, suggesting a likely
association with the diseased state [24, 25]. The relevance of the high frequency detected of
respiratory viruses such as Rhinovirus in single and co-infections requires further investigation.
Because of the low numbers of each specific co-infection combination, we did not report on
clinical outcomes and how single and co-infections differed from each other clinically. This study
also did not include bacterial testing and therefore gaps remain in our understanding of all the
aetiologies of SARI in South Africa. Lastly, the study period of 2 years could also be a limitation
since the circulation of viruses could change from year to year, however only two years of data
is represented here, the surveillance study is on-going and changes in the seasonal circulation
of the viruses will be detected. Understanding the contribution these viruses to severe
respiratory disease will allow for informed decision making when selecting specific respiratory
pathogens as part of sustainable respiratory disease surveillance. Currently the overall cost of
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the assay from extraction to detection is USD 63, with a panel (consisting of two to three
viruses) costing USD 12 however, by selecting only the major contributors to SARI, the cost of
running the assay could be reduced in future.
In conclusion this study indicates a contributing role for co-infecting viruses in patients
presenting with SARI, and highlights the important role of viral co-infection. Continued use of
the rRT-PCR multiplex assay in conjunction with the SARI surveillance programme will enhance
our ability to detect circulation of respiratory viruses in patients hospitalised for SARI and help to
clarify the contribution of these respiratory viruses among patients with SARI in South Africa.
Acknowledgments
This surveillance study has been funded by a co-operative agreement with the Centers for
Disease Control and Prevention, Atlanta, Georgia, USA. We would like to thank the following
Units and individuals at the National Institute for Communicable Diseases, a division of the
National Health Laboratory Service: Executive Director: Barry Schoub, Epidemiology and
Surveillance Unit: Lucille Blumberg, Babatyi Malope-Kgokong, Jo McAnerney, Locadiah
Mlambo, Sindile Ntuli,. Respiratory and Meningeal Pathogens Reference Unit: Anne von
Shangase. All the patients who kindly agreed to participate in the surveillance.
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