Resistance profiles of Pseudomonas aeruginosa isolates from various water environments including swimming pools O. Pappa 1,3,4 , A. Vantarakis 2 , A.M. Kefala 4 , A.Mavridou 3 , A. Galanis 4 1 Central Public Health Laboratory, Hellenic Centre for Diseases Control and Prevention, Athens, Greece 2 Environmental Microbiology Unit, Department of Public Health, School of Medicine, University of Patras, Greece 3 Technological Educational Institute of Athens, Department of Medical Laboratories, Athens, Greece 4 Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece *This work is funded by State Scholarship Foundation, 41, Ethnikis Antistaseos Ave, PoBox142 34, Nea Ionia, Athens,GR
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Resistance profiles of Pseudomonas aeruginosa isolates from various water environments including swimming pools
O. Pappa1,3,4 , A. Vantarakis2, A.M. Kefala4, A.Mavridou3, A. Galanis4
1Central Public Health Laboratory, Hellenic Centre for Diseases Control and Prevention, Athens, Greece
2Environmental Microbiology Unit, Department of Public Health, School of Medicine, University of Patras, Greece
3Technological Educational Institute of Athens, Department of Medical Laboratories, Athens, Greece
4Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece
*This work is funded by State Scholarship Foundation, 41,
Ethnikis Antistaseos Ave, PoBox142 34, Nea Ionia,
Athens,GR
Pseudomonas aeruginosa and Resistance Mechanisms
The study of resistant P. aeruginosa isolates circulating in various aquatic
habitats is profoundly important to public health, but the environmental
reservoirs of resistance factors such as swimming pools are poorly
understood.
acquired resistance mechanisms
an extensive efflux pump
system
intrinsic antimicrobial resistance:
low outer membrane
permeability
chromosomally
encoded AmpC
transposons integrons plasmids
Pseudomonas aeruginosa and Resistance Mechanisms
Reduced outer-membrane permeability + increase in drug efflux
Multi Drug Resistant isolates (MDR)
(Livermore, 2011; Sanchez, 2014)
Resistant P. aeruginosa isolates
pool water samples biofilms
potential mechanisms of bacteria
resistance against chlorine
(Grobe, 2001; Venezia, 2005)
Pseudomonas aeruginosa and Resistance Mechanisms
In Greece, studies on P. aeruginosa isolated from pools have been published,
but these studies outlined the resistance of the bacterium in commonly used
antibiotics only (Papadopoulou, 2008; Tirodimos, 2008)
beta-lactamases
Extended Spectrum Beta-
Lactamases (ESBLs)
Metalo Beta-Lactamases
(MBL)
(Zhang, 2013; Maravic, 2012)
Aim of the study
Since the detection of P. aeruginosa is required by the current legislation in
swimming pools, it is very important to elucidate the resistance mechanisms
circulating in aquatic environments and specifically in swimming pools.
Multi Locus Sequence Typing (MLST): a typing method focusing strictly on 7
conserved housekeeping genes was chosen for the typing and the discrimination
of the isolates. The behavior of the resistant isolates when MLST is applied