Research in Tuberculosis: Research in Tuberculosis: Translation into Practice Translation into Practice Michael Lauzardo, MD, MSc Michael Lauzardo, MD, MSc Principal Investigator, Southeastern National Tuberculosis Cente Principal Investigator, Southeastern National Tuberculosis Center Assistant Professor, Div. of Pulmonary and Critical Care Medicin Assistant Professor, Div. of Pulmonary and Critical Care Medicine, e, University of Florida College of Medicine University of Florida College of Medicine Deputy Health Officer for TB Deputy Health Officer for TB – State of Florida State of Florida Case Case – History History y This is a 6 This is a 6- year year- old Bosnian male, who presented old Bosnian male, who presented to ER with one to ER with one- week history of fever and occasional week history of fever and occasional vomiting. No cough, difficulty breathing or weight loss. vomiting. No cough, difficulty breathing or weight loss. y Evaluation revealed right lobar pneumonia. Evaluation revealed right lobar pneumonia. Patient was admitted to the hospital and started on IV Patient was admitted to the hospital and started on IV antibiotics (Ceftriaxone). antibiotics (Ceftriaxone). Case Case – History History y PMH PMH – No significant illnesses in the past, no surgeries. No significant illnesses in the past, no surgeries. y Social History Social History – Patient lives with both parents and a younger sibling. Patient lives with both parents and a younger sibling. They all immigrated to U.S. from Bosnia about six They all immigrated to U.S. from Bosnia about six months PTA. At that time, they all had PPD placed. months PTA. At that time, they all had PPD placed. Both parents tested positive, but elected not to take Both parents tested positive, but elected not to take meds. Both children tested negative. meds. Both children tested negative. Case Case – Physical Examination Physical Examination y T: 103.2 T: 103.2° y P: 154 P: 154 y R: 24 R: 24 y BP: 111/70. BP: 111/70. y O2 Sat. on RA: 96 O2 Sat. on RA: 96–97%. 97%. y Patient was awake, Patient was awake, alert, in no apparent distress alert, in no apparent distress y Chest Chest – Markedly decreased Markedly decreased BS in the right lung field BS in the right lung field y Rest of exam was within Rest of exam was within normal limits normal limits Case Case y CXR CXR – PA PA y Large opacity that filled the lower 2/3 of the right hemithorax, Large opacity that filled the lower 2/3 of the right hemithorax, heart slightly displaced to the left, heart slightly displaced to the left, clear left lung field. clear left lung field. – Right lateral decubitus Right lateral decubitus y Mass or large organized pleural effusion without Mass or large organized pleural effusion without free layering. free layering. 2008 HEALTH RESEARCH IN THE AMERICAS IV: HIV/TB MODULE 5 TRANSLATION TO PRACTICE PAGE 1
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Research in Tuberculosis:Research in Tuberculosis:Translation into PracticeTranslation into Practice
Michael Lauzardo, MD, MScMichael Lauzardo, MD, MScPrincipal Investigator, Southeastern National Tuberculosis CentePrincipal Investigator, Southeastern National Tuberculosis CenterrAssistant Professor, Div. of Pulmonary and Critical Care MedicinAssistant Professor, Div. of Pulmonary and Critical Care Medicine,e,
University of Florida College of MedicineUniversity of Florida College of MedicineDeputy Health Officer for TB Deputy Health Officer for TB –– State of FloridaState of Florida
Case Case –– History History
This is a 6This is a 6--yearyear--old Bosnian male, who presented old Bosnian male, who presented to ER with oneto ER with one--week history of fever and occasional week history of fever and occasional vomiting. No cough, difficulty breathing or weight loss. vomiting. No cough, difficulty breathing or weight loss.
Evaluation revealed right lobar pneumonia. Evaluation revealed right lobar pneumonia. Patient was admitted to the hospital and started on IV Patient was admitted to the hospital and started on IV antibiotics (Ceftriaxone).antibiotics (Ceftriaxone).
Case Case –– HistoryHistory
PMHPMH
–– No significant illnesses in the past, no surgeries.No significant illnesses in the past, no surgeries.
Social HistorySocial History
–– Patient lives with both parents and a younger sibling. Patient lives with both parents and a younger sibling. They all immigrated to U.S. from Bosnia about six They all immigrated to U.S. from Bosnia about six months PTA. At that time, they all had PPD placed. months PTA. At that time, they all had PPD placed. Both parents tested positive, but elected not to take Both parents tested positive, but elected not to take meds. Both children tested negative. meds. Both children tested negative.
Case Case –– Physical ExaminationPhysical Examination
T: 103.2T: 103.2°°
P: 154P: 154
R: 24R: 24
BP: 111/70.BP: 111/70.
O2 Sat. on RA: 96O2 Sat. on RA: 96––97%.97%.
Patient was awake, Patient was awake, alert, in no apparent distressalert, in no apparent distress
ChestChest
–– Markedly decreased Markedly decreased BS in the right lung fieldBS in the right lung field
Rest of exam was within Rest of exam was within normal limitsnormal limits
Case Case
CXRCXR
–– PAPA
Large opacity that filled the lower 2/3 of the right hemithorax,Large opacity that filled the lower 2/3 of the right hemithorax,heart slightly displaced to the left, heart slightly displaced to the left, clear left lung field. clear left lung field.
–– Right lateral decubitusRight lateral decubitus
Mass or large organized pleural effusion without Mass or large organized pleural effusion without free layering.free layering.
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Case Case
Patient continued having daily fever spikes, but Patient continued having daily fever spikes, but without changes in his respiratory status.without changes in his respiratory status.
–– Would you do any further evaluation?Would you do any further evaluation?
–– Repeat TST?Repeat TST?
–– Do we need gastric aspirates versus sputum?Do we need gastric aspirates versus sputum?
–– Spinal tap?Spinal tap?
–– Other cultures?Other cultures?
–– Other tests?Other tests?
Case Case –– Hospital CourseHospital Course
TST was placed and read in 48 hours as 22 mm of induration. TST was placed and read in 48 hours as 22 mm of induration.
Induced sputum/early morning gastric aspirates were obtained. Induced sputum/early morning gastric aspirates were obtained.
Chest US showed a loculated, organized pleural effusionChest US showed a loculated, organized pleural effusion
Surgery was consulted and they performed a videoSurgery was consulted and they performed a video--assisted assisted thoracotomy and decortication. Pleural biopsy and AFB cultures thoracotomy and decortication. Pleural biopsy and AFB cultures were sent.were sent.
HIV antibody: negative HIV antibody: negative
Case Case –– Hospital CourseHospital Course
Patient was started on INH, Rif, PZA and Ethambutol.Patient was started on INH, Rif, PZA and Ethambutol.
He improved with resolution of fever in three days. He improved with resolution of fever in three days.
He was discharged home to continue meds under DOT.He was discharged home to continue meds under DOT.
Case Case –– Final ResultsFinal Results
Gastric aspirates X 3Gastric aspirates X 3
–– AFB smear and cultures were negativeAFB smear and cultures were negative
Induced sputum AFB smear was negative Induced sputum AFB smear was negative
OneOne--third of the worldthird of the world’’s population infecteds population infected
Eight million new cases of active disease per yearEight million new cases of active disease per year
Two to three million deaths per yearTwo to three million deaths per year
One person is newly infected every second and one person dies One person is newly infected every second and one person dies every 10 secondsevery 10 seconds
Rising incidence of drugRising incidence of drug--resistant diseaseresistant disease
Billions of dollars in lost productivityBillions of dollars in lost productivity
Global TB RatesGlobal TB Rates Tuberculosis in the United States: Epidemic Tuberculosis in the United States: Epidemic Under ControlUnder Control
–– Disastrous outcomes in HIV settingsDisastrous outcomes in HIV settings
FirstFirst--line Therapy for Cases of Drug Susceptible TBline Therapy for Cases of Drug Susceptible TB
IsoniazidIsoniazid
RifamycinsRifamycins
PyrazinamidePyrazinamide
EthambutolEthambutol
AminoglycosidesAminoglycosides
CapreomycinCapreomycin
QuinolonesQuinolones
ThioamidesThioamides
CycloserineCycloserine
PASPAS
Therapy is standardizedTherapy is standardized
4 drugs for 64 drugs for 6--9 months9 months
Safe effective and cheapSafe effective and cheap
95% cure rate 95% cure rate
Cost about $20Cost about $20
Extensive evidence base of Extensive evidence base of RCTsRCTsto support practiceto support practice
SecondSecond--line Drugs for Drug Resistant TBline Drugs for Drug Resistant TB
IsoniazidIsoniazid
RifamycinsRifamycins
PyrazinamidePyrazinamide
EthambutolEthambutol
AminoglycosidesAminoglycosides
CapreomycinCapreomycin
QuinolonesQuinolones
ThioamidesThioamides
CycloserineCycloserine
PASPAS
Need lab testing and or Need lab testing and or epiepiinformation on prevalent resistant information on prevalent resistant strainsstrains
44--6 drugs for 2 years6 drugs for 2 years
Less effectiveLess effective
<80% cure rate<80% cure rate
$3500 to $5000 cost for drugs$3500 to $5000 cost for drugs
No clinical trials to supportNo clinical trials to support
Extensively Drug Resistant TB Extensively Drug Resistant TB XDR TBXDR TB
Results from inadequate MDR TB treatment and leads to Results from inadequate MDR TB treatment and leads to secondsecond--line drug resistanceline drug resistance
Defined from October 2006 as being:Defined from October 2006 as being:
–– MDR TB plus resistance to at least a fluoroquinolone and any MDR TB plus resistance to at least a fluoroquinolone and any sescondsescond--line injectable (amikacin, kanamycin, and capreomycin)line injectable (amikacin, kanamycin, and capreomycin)
–– If Fluoroquinolone and injectable are available then If Fluoroquinolone and injectable are available then outcmeoutcme of of MDR is better (69% cure rate MDR is better (69% cure rate vsvs 30%)30%)
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XDR TB Outbreak, South AfricaXDR TB Outbreak, South Africa20052005--20062006
119 patients in TB/ARV integration study119 patients in TB/ARV integration study
–– 14 deaths of which 10 were MDR14 deaths of which 10 were MDR
–– 6 of 10 isolates were resistant to all the drugs tested6 of 10 isolates were resistant to all the drugs tested
Suggested that the facility likely had high rates of resistanceSuggested that the facility likely had high rates of resistance
Prompted a survey from Jan 2005 through March 2006Prompted a survey from Jan 2005 through March 2006
South Africa Drug Resistance Survey South Africa Drug Resistance Survey
Of 542 M. TB cultures 221 (41%) Of 542 M. TB cultures 221 (41%) were MDR TBwere MDR TB
53 (10%) XDR TB53 (10%) XDR TB
52 of the 53 patients died within 52 of the 53 patients died within 25 days of the diagnosis25 days of the diagnosis
All were HIV positive and those All were HIV positive and those that died included those on antithat died included those on anti--retroviral therapyretroviral therapy
XDR likely has spread to XDR likely has spread to neighboring African countriesneighboring African countries
From NEJM 356;7:2007
TB Drug ResistanceTB Drug Resistance Global Burden of Drug Resistant TBGlobal Burden of Drug Resistant TB
The XDR outbreak in South Africa led to prevalence surveys of The XDR outbreak in South Africa led to prevalence surveys of national labsnational labs
The global burden of MDR or XDR TB is now estimated to be The global burden of MDR or XDR TB is now estimated to be over 450,000over 450,000
Over 28 countries now have confirmed cases of XDR with likely Over 28 countries now have confirmed cases of XDR with likely spread in subspread in sub--Saharan Africa in populations with high rates of Saharan Africa in populations with high rates of HIV HIV
HIV / TB CoHIV / TB Co--InfectionInfection
HIV and TB:HIV and TB:Twin EpidemicsTwin Epidemics
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HIV and TBHIV and TB
The twin epidemics of HIV and TB are the single biggest threat The twin epidemics of HIV and TB are the single biggest threat to global TB controlto global TB control
The WHO strategy of DOTS fails to decrease the incidence of TB The WHO strategy of DOTS fails to decrease the incidence of TB in settings of high HIV prevalencein settings of high HIV prevalence
Expansion of ARV therapy is good overall for TB control efforts Expansion of ARV therapy is good overall for TB control efforts however, it may lead to drug interactions that may worsen MDR however, it may lead to drug interactions that may worsen MDR TB epidemicTB epidemic
May lead to a paradoxical worsening of the TB burden by May lead to a paradoxical worsening of the TB burden by improving survival in HIV positive individuals who would be at improving survival in HIV positive individuals who would be at risk for TB risk for TB
Poverty and TBPoverty and TB
Responding to the ChallengesResponding to the Challenges
Technological AdvancesTechnological Advances
Whole Blood GammaWhole Blood GammaInterferon Assay for LTBIInterferon Assay for LTBI
Quantiferon gold recently approved by FDA utilizing early Quantiferon gold recently approved by FDA utilizing early secretory antigenic target 6 (ESATsecretory antigenic target 6 (ESAT--6) and culture filtrate protein 6) and culture filtrate protein 10 (CFP 10) [present in TB and bovis absent from BCG and 10 (CFP 10) [present in TB and bovis absent from BCG and most NTM except kansasii, szulgai and marinum) by ELISAmost NTM except kansasii, szulgai and marinum) by ELISA
May be able to discern reaction to BCG and NTMMay be able to discern reaction to BCG and NTM
More studies needed to discern role in LTBI diagnosisMore studies needed to discern role in LTBI diagnosis
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Vaccine HistoryVaccine History
BCG was developed by Albert Calmette and Camille Guerin who BCG was developed by Albert Calmette and Camille Guerin who attenuated a strain of M. bovis by growing it on culture medium attenuated a strain of M. bovis by growing it on culture medium for 13 years.for 13 years.
Virulence was monitored in animal models.Virulence was monitored in animal models.
In 1921 it was first administered widely in France where it In 1921 it was first administered widely in France where it reduced infant mortality from TB by 90%.reduced infant mortality from TB by 90%.
BCG TodayBCG Today
Now one of the most widely used vaccinesNow one of the most widely used vaccines
Very effective at reducing mortality in infantsVery effective at reducing mortality in infants
Protective effect lasts no more than 10Protective effect lasts no more than 10--15 years at best15 years at best
Efficacy has been shown to be highly variable (0Efficacy has been shown to be highly variable (0--80%) in 80%) in protecting against TB diseaseprotecting against TB disease
Reasons for this are unclear but likely involve the relationshipReasons for this are unclear but likely involve the relationships s between M. tuberculosis, BCG, and other environmental nonbetween M. tuberculosis, BCG, and other environmental non--tuberculous mycobacteriatuberculous mycobacteria
Immunology of TBImmunology of TB
Doherty et al. Clin Micro Rev. 2005 Vol. 18, p 687-702
Vaccine SummaryVaccine Summary
Unexpected advances in the field of immunology and vaccine Unexpected advances in the field of immunology and vaccine development hold great promise for the delivery of the most development hold great promise for the delivery of the most important new tool for the elimination of TB.important new tool for the elimination of TB.
Without a vaccine, the elimination of TB is likely impossible.Without a vaccine, the elimination of TB is likely impossible.
The current vaccine candidates are not ideal but even a less The current vaccine candidates are not ideal but even a less than ideal vaccine can potentially become a costthan ideal vaccine can potentially become a cost--effective effective valuable tool that can be applied globally.valuable tool that can be applied globally.
Diagnosis of TB in LowDiagnosis of TB in Low--Resource SettingsResource Settings MicroscopicMicroscopic--Observation DrugObservation Drug--Susceptibility TestingSusceptibility TestingMODSMODS
MODS is a rapid lowMODS is a rapid low--cost technique in which broth cultures are cost technique in which broth cultures are examined microscopically to detect characteristic growthexamined microscopically to detect characteristic growth
In a recent study in Peru the MODS assay was compared headIn a recent study in Peru the MODS assay was compared head--toto--head with two reference methodshead with two reference methods
Of 3760 sputum samples, 401(10.7%) yielded positive cultures Of 3760 sputum samples, 401(10.7%) yielded positive cultures for MTBfor MTB
Sensitivity of detection was 97.8% for MODSSensitivity of detection was 97.8% for MODS
Agreement between MODS and reference standard for Agreement between MODS and reference standard for susceptibility was 100% for rifampin, 97% for isoniazid, and susceptibility was 100% for rifampin, 97% for isoniazid, and 99% for both 99% for both
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The Effect of DOTS ExpansionThe Effect of DOTS Expansion
Global Collaborations Global Collaborations in the Fight Against TBin the Fight Against TB
Goals of the STOP TB PartnershipGoals of the STOP TB Partnership
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Developing New Drugs for TBDeveloping New Drugs for TB
New TB Drug PipelineNew TB Drug Pipeline
Early Bactericidal ActivityEarly Bactericidal Activity
In EBA studies, newly diagnosed patients with acidIn EBA studies, newly diagnosed patients with acid--fast fast bacillus (AFB)bacillus (AFB)--positive, pulmonary tuberculosis are treated for positive, pulmonary tuberculosis are treated for periods ranging from 2 to 14 days with single drugs or drug periods ranging from 2 to 14 days with single drugs or drug combinations. combinations.
During this period, quantitative counts of viable tubercle bacilDuring this period, quantitative counts of viable tubercle bacilli li from carefully collected sputum specimens are made, with the from carefully collected sputum specimens are made, with the EBA traditionally expressed as logEBA traditionally expressed as log--decrease in colony forming decrease in colony forming units/ml sputum/day over the first 48 hours,units/ml sputum/day over the first 48 hours,
Generally this technique is used as a proof of concept.Generally this technique is used as a proof of concept.
Usually follows inUsually follows in--vitro and animal studies.vitro and animal studies.
What are clinical trial phases?What are clinical trial phases?
Phase 1 clinical trials, researchers test a new drug or Phase 1 clinical trials, researchers test a new drug or treatment in a small group of people (20treatment in a small group of people (20--80) for the first time 80) for the first time to evaluate its safety, determine a safe dosage range, and to evaluate its safety, determine a safe dosage range, and identify side effects.identify side effects.
Phase 2 clinical trials, the treatment is given to a larger grouPhase 2 clinical trials, the treatment is given to a larger group p of people (40of people (40--100) to see if it is effective and to further 100) to see if it is effective and to further evaluate its safety.evaluate its safety.
Phase 3 studies, the study drug or treatment is given to large Phase 3 studies, the study drug or treatment is given to large groups of people (more than 200) to further determine its groups of people (more than 200) to further determine its effectiveness, monitor side effects, and compare it to effectiveness, monitor side effects, and compare it to commonly used treatments, commonly used treatments,
Effective against DSEffective against DS--TB and TB and MDRMDR--TB as well as other TB as well as other clinically important clinically important Mycobacterial speciesMycobacterial species
Has a novel mechanism of Has a novel mechanism of actionaction
Phase I studies among Phase I studies among healthy males was very well healthy males was very well toleratedtolerated
Phase II studies about to Phase II studies about to begin in MDRbegin in MDR--TB patientsTB patients
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Pyrrole (LL3858)Pyrrole (LL3858)
Currently being evaluated in a Phase I study in India with Currently being evaluated in a Phase I study in India with healthy volunteers.healthy volunteers.
Mechanism of action is uncertain but has MICMechanism of action is uncertain but has MIC’’s for DSs for DS--TB and TB and MDRMDR--TB are similar,TB are similar,
The sterilizing activity of this agent in the spleen and lungs oThe sterilizing activity of this agent in the spleen and lungs of f mice is faster than that of the conventional INH/RIF/PZA mice is faster than that of the conventional INH/RIF/PZA regimen.regimen.
Also has some effect against nonAlso has some effect against non--TB mycobacterial species.TB mycobacterial species.
QuinolonesQuinolones
Originally looked at to treat Originally looked at to treat MDRMDR--TB.TB.
More interest now in its role More interest now in its role to shorten the regimen.to shorten the regimen.
Report from Chennai India Report from Chennai India showed remarkable sputum showed remarkable sputum conversion rates at 2 conversion rates at 2 months (92%months (92%--98% rather 98% rather than expected 80% with than expected 80% with conventional therapyconventional therapy).).
An Alternative View:An Alternative View:Back to the FutureBack to the Future
Who says that currently available drugs are not sufficient for Who says that currently available drugs are not sufficient for treating drug resistant TB?treating drug resistant TB?
In the 1960In the 1960’’s it took 8 years to get a new drug to market, now s it took 8 years to get a new drug to market, now it takes 20 years.it takes 20 years.
The data to justify the current dosing is weak and there is littThe data to justify the current dosing is weak and there is little le evidence to support our current strategiesevidence to support our current strategies
Would it not make sense to fund studies to evaluate the dose Would it not make sense to fund studies to evaluate the dose used in currently used regimens while we wait for new drugs?used in currently used regimens while we wait for new drugs?
ConclusionConclusion
2008 HEALTH RESEARCH IN THE AMERICAS IV: HIV/TB MODULE 5 TRANSLATION TO PRACTICE