Reporter : Hsun-Pei Wu Advisor : Cheng-Hsin Chuang Department of Mechanical Engineering & Institute of Nanotechnology, Southern Taiwan University, Tainan, TAIWAN March 28th Enhancement of Fluorescent Intensity by Using DEP Manipulations of Polyaniline-coated Al 2 O 3 Nanoparticles for Immunosensing
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Reporter : Hsun-Pei Wu Advisor : Cheng-Hsin Chuang Department of Mechanical Engineering & Institute of Nanotechnology, Southern Taiwan University, Tainan,
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Reporter : Hsun-Pei Wu
Advisor : Cheng-Hsin Chuang
Department of Mechanical Engineering & Institute of Nanotechnology,
Dielectrophoresis- Motion of a particle produced by the interaction of a Non-uniform electric field with the induced effective dipole moment of the particle.
23)*m
2ε*P
ε
*m
ε*p
εRe(2 ErF mDEP
Clausius-Mossotti factor
Re(fCM)>0 ; Positive DEP
Re(fCM)<0 ; Negative DEP
Four Different forms of PANIFour Different forms of PANI
Reduced State
Oxidized State
Redox StateRedox State FormsForms
Pernigraniline Base(PNB)
Metallic Emeraldine Salt (ES)
Emeraldine Base (EB)
Leuco emeraldine Base (LEB)
Chemical FormulaChemical Formula
Dodecyl Benzene Sulfonic Acid (DBSA)
HClAcOH H3PO4DBSA
104 S/cm101 S/cm
Doping Different Acid
Doping Different Acid
Without corrosion of metal electrodes by the residual acidGood AdhesionBetter soluble for synthesis
Without corrosion of metal electrodes by the residual acidGood AdhesionBetter soluble for synthesis
Scheme of the molecular formula of PANDB
TEM image of PANDB
Different Forms Different Electrical Properties
Synthesis of AlSynthesis of Al22OO33 NPs NPs
TEM Image of PANDB-coated AlTEM Image of PANDB-coated Al22OO33 NPs NPs
SEM Image of Al2O3 NPs SEM Image of PANDB-Al2O3 NPs
With DEP
Without DEP
Normalized fluorescent intensity (It/It=0) for different immunosensing methods within 60 minutes, Solid lines represent the immunosensing with DEP force and dashed lines are without DEP as immunosensing
Normalized fluorescent intensity (It/It=0) for different immunosensing methods within 60 minutes, Solid lines represent the immunosensing with DEP force and dashed lines are without DEP as immunosensing
Silane-Al2O3-NPs are trapping with /without DEP
Silane-Al2O3-NPs are trapping with /without DEP
PANDB-Al2O3-NPs are trapping with /without DEP
PANDB-Al2O3-NPs are trapping with /without DEP
1 Times
Without DEP
With DEP
4 Times
0 10 20 30 40 50 60
Im m unosensing Tim e (m in)
0
2
4
6
8
No
rmal
ize
d F
luo
res
cen
t In
ten
sity
( I
t /
I t =
0 )
Comparison between Difference Methods Silane - Al2O 3 - Antibody - BSA ( without DEP )
Silane - Al2O 3 - Antibody - BSA ( with DEP )
[ PANDB-Al2O 3 ] - Antibody - BSA ( without DEP )
[ PANDB-Al2O 3 ] - Antibody - BSA ( with DEP )
Saturated after 20 min
Experimental Result-Experimental Result- (2) Effect of Immunosensing Time
ConclusionsConclusions We developed a DEP chip with a dot-electrode array for the condensation
of NPs and the enhancement of fluorescent intensity under a programmable control system.
According to the experimental results of fluorescent intensities, the intensity increased with either of applied time and immunosensing with p-DEP force.
This technology could apply to the biosensor such as immunoassay when the immobilized NPs as specific biomarkers.
In the future, our biosensor can integrate with PANDB material and impedance detection on to the immunoassay.