Report on NANO International Training course on “Basic sampling protocols for Harmful Algal Blooms (HAB) studies: taxonomy and toxin” Venue: Regional Centre of CMFRI, Mandapam Dates: 12 – 15 May 2015 different NANO International Training course on “Basic sampling protocols for Harmful Algal Blooms (HAB) studies: taxonomy and toxin” [as part of the NANO regional project “Study of Harmful Algal Blooms and other Aspects of Sardine Habitats around the Indian Sub-continent (SHABASHI)”] was organized by Nansen Environmental Research Centre India (NERCI) in association with Central Marine Fisheries Research Institute (CMFRI) at Mandapam Regional Centre of CMFRI from 12th to 15th May 2015. The training was conceived as an informal one to obtain clarity regarding the sampling, methodology and parameters to be measured. However, the arrangements that had to be made at the Mandapam regional centre of CMFRI gave it a formal status and the programme was inaugurated by Dr. A. K. Abdul Nazar, Scientist-in-Charge & Organizing Secretary of the workshop. Dr. Nandini Menon, Scientist, NERCI & Co-leader of SHABASHI gave an overview of the project as well as the training workshop. Dr. B. Johnson, Scientist, CMFRI Mandapam, delivered the welcome address and Dr.Grinson George, Senior Scientist & Patron of the training programme proposed the vote of thanks. Dr. A. K. Abdul Nazar, Scientist in Charge & Organizing Secretary delivering the inaugural address
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Report on NANO International Training course on “Basic sampling protocols for Harmful Algal Blooms (HAB) studies: taxonomy and toxin”
Venue: Regional Centre of CMFRI, Mandapam
Dates: 12 – 15 May 2015 different
NANO International Training course on “Basic sampling protocols for Harmful Algal Blooms (HAB) studies: taxonomy and toxin” [as part of the NANO regional project “Study of Harmful Algal Blooms and other Aspects of Sardine Habitats around the Indian Sub-continent (SHABASHI)”] was organized by Nansen Environmental Research Centre India (NERCI) in association with Central Marine Fisheries Research Institute (CMFRI) at Mandapam Regional Centre of CMFRI from 12th to 15th May 2015. The training was conceived as an informal one to obtain clarity regarding the sampling, methodology and parameters to be measured. However, the arrangements that had to be made at the Mandapam regional centre of CMFRI gave it a formal status and the programme was inaugurated by Dr. A. K. Abdul Nazar, Scientist-in-Charge & Organizing Secretary of the workshop. Dr. Nandini Menon, Scientist, NERCI & Co-leader of SHABASHI gave an overview of the project as well as the training workshop. Dr. B. Johnson, Scientist, CMFRI Mandapam, delivered the welcome address and Dr.Grinson George, Senior Scientist & Patron of the training programme proposed the vote of thanks.
Dr. A. K. Abdul Nazar, Scientist in Charge & Organizing Secretary delivering the inaugural address
The list of participants was as follows:
No. Name Affilaition Mail id1 Prof. Kanthi
K.A.S.YapaDepartment of PhysicsUniversity of RuhunaMatara, Sri Lanka
[email protected]@phy.ruh.ac.lk
2 Dr. Nandini Menon ScientistNERCI,Kochi 16
3 Dr. H.B. Jayasiri NARACrow IslandColombo 15Sri Lanka
4 Dr. H.B. Asanthi Department of LimnologyFaculty of Fisheries and Marine Science,University of Ruhana
5 Dr. Gunjan Motwani Space application centreISRO- Ahmedabad
6 Loveson Edward L. ScientistVRC of CMFRI,Visakhapatanam
7 Mathew K.A. Research Scholar, CUSAT, NERCI, Kochi
8 Nashad M. Nansen Research Fellow, CUSAT, Kochi
9 Dr. Amir Kumar Samal ScientistMRC of CMFRI,Mandapam
10 Shaju S.S. Research FellowPOGO NANO project SHABASHI, NERCI, Kochi
11 Dr. I. Rajendran ScientistMRC of CMFRI,Mandapam
12 Dr. Grinson George Sr.ScientistCMFRI,Kochi
13 Ravi K.Avadhanula Technical AssistantMRC of CMFRI,Mandapam
14 M. Gopala Krishnan Senior Research Fellow, MRC of CMFRI,Mandapam
15 Minu P. Senior Research Fellow,Fishing technology DivisionCentral Institute of Fisheries and TechnologyKochi
Participants of the training programme in front of the CMFRI mandapam Centre
Day 1, 12.05.15
Tuesday, 12th May, 2015Time Resource person Topics*09:30 to 10:30 Inauguration- Scientist-in-charge, CMFRI, Mandapam10:30 to 11:00 Tea break11:00 to 12:30 Dr. Nandini Menon Basics of HABs, Toxin and global
scenario12:30 to 2.00 Lunch Break2.00 to 5:30 Shaju. S. S Bio-optical measurements of HAB,
Sampling Protocol for SHABASHI
After the formal inauguration, Dr. Nandini Menon gave the introductory lecture on the topic “Basics of HABs, Toxin and global scenario” wherein she presented the different types of phytoplankton groups which are commonly represented in HAB incidents, the HAB records in India, studies undertaken on HAB aspects and compared it with the South East Asian scenario where HABs, toxin release and fish kills are common phenomena. Afternoon sessions were practical oriented and Mr. Shaju S. S. began with the method of sampling for bio-optical analysis. The first day concluded with the following actions on the participants:
Action Shaju: Make a protocol /manual listing step by step details of sample collection and analysis and circulate it as pdf among the participants.
Action Mathew: IOCCG report No. 3 which gives the analytical protocols for bio-optical measurements to be circulated among the participants.
Action Nandini, Grinson: Use the HAB database available with NERCI, convert the point data to climatology, prepare a composite picture combining environmental data and HAB data and come out with a publication.
Dr. Nandini Menon lecturing on HAB types and global scenario
Day 2. 13.05.15
Wednesday, 13th May, 2015Time Resource person Topics07:00 to 11:30 Cruise and collection of samples11:30 to 1.30 Lunch Break1.30 – 3.30 Shaju S.S./ Dr. Gunjan
MotwaniPre-processing, subsampling , physico chemical and Bio-optical analysis
3:30 to 4:00 Tea break
4:00 to 6:00 Minu P. Phytoplankton taxonomy andEnumeration - practicals
Sampling by the participants on 13.05.2015
All the participants went to the sea for sampling in two outboard boats at 7.00 am from the CMFRI jetty. Water samples were collected for the analysis of bio-optical parameters, phytoplankton composition and enumeration and pigment analysis by HPLC.
Immediately after reaching the laboratory, samples were filtered for bio-optical and HPLC analyses. Shaju S.S. along with Gunjan Motwani headed the team and hands on training weregiven to all participants.
Identification of phytoplankton using microscopy was done simultaneously under the guidance of Ms. Minu P.
Phytoplankton taxonomy theory and practical by Minu. P
Demonstration of Bio-optical analysis by Shaju S.S.
Second day of the workshop ended with the following actions on the members:Action Kanthi: Procure a 25 mm filtration unit for the filtration of SriLankan samples and get it reimbursed from NANO funds (through Nandini).
Action Grinson: Provide 25 mm filtration unit for Vizag CMFRI.
Action Amir: Procure GF/F and 0.2 µ filter papers for Mandapam centre.
Day 3, 14.05.15
Thursday, 14th May, 2015Time Resource person Topics07:00 to 09:00 Dr. Gunjan Motwani Basics of HPLC pigment analysis and
sample preparation. 09:00 to 10.00 Tea break10.00 to 12:30 Minu P. Phytoplankton taxonomy and
analysis12:30 to 2:00 Lunch Break2.00 to 3.30 Dr. Gunjan Motwani HPLC pigment analysis3.30 to 4.00 Tea break4.00 to 7.00 Dr. Gunjan Motwani HPLC pigment analysis
HPLC pigment analysis demonstration by Dr. Gunjan Motwani on 14.05.15
On day 3, the focus was on the HPLC pigment analysis by Dr. Gunjan Motwani. Dr. Gunjan explained the principle of HPLC, the components of the equipment and the method of operation. After the theory, Dr. Gunjan along with Dr. Amir, scientist at CMFRI, Mandapm demonstrated the extraction of pigment from the sample, and its analysis using HPLC. The various problems encountered in the HPLC operation as well as the adoption of suitable methodology was discussed in detail and by the end of the day, the following conclusions and actions were made.
In between the HPLC run, the time was utilized for a theory lecture by Minu. P on phytoplankton taxonomy.
Action Gunjan: Provide the detailed methodology to Amir so that the pigment analysis at mandapam would also be done using the Zapata (2000) method.
Action Nandini/Shaju: Provide C8 with reverse phase polymerase type columns and working standard on chlorophyll for Mandapam CMFRI.
Action Grinson: Provide a dual beam UV/Visible spectrophotometer to Mandapam CMFRI.
Action Ravidas: Methodology for culturing toxic algae to be sent to Mandapam CMFRI.
Day 4,15.05.15
Friday, 15th April, 2015
Time Resource person Topics07:00 to 09:00 Dr. Nandini Menon,
Dr.Kanthi YappaLecture and demonstration of SPATT bags for toxin analysis
09:00 to 10.00 Tea break10.00 – 11.30 Shaju S.S. HAB Bio-optics calculation and analysis11:30 to 1.00 Dr. Gunjan Motwani Pigment analysis by HPLC1.00 to 2.00 Dr. Grinson George Poster for Blue planet symposium and
presentation at the POGO meeting2.00 – 3.00 Lunch break3:00 to 4.00 Dr. Gunjan Motwani Community Structure analysis4.00 to 4.30 Valedictory function4.30 – 6.00 Visit around the Mandapam centre of CMFRI
A lecture on SPATT bags for toxin analysis was given by Dr. Nandini Menon and Dr. Kanthi Yappa demonstrated the preparation of the SPATT bag, its deployment, retrieval, and preservation before sending the sample for analysis to Rajdeep. Shaju S.S taught the calculation of the concentration of bio-optical parameters from the absorbances measured. The various methods used for the estimation of phytoplankton community structure from the pigment data was presented by Dr. Gunjan Motwani.
Dr. Kanthi Yapa demonstrating the SPATT bag technique for toxin analysis
The rest of the morning was dedicated to discussion on the poster to be put up at the Blue planet symposium. It was decided that the poster would have the following contents:
1) The abstract in a concise and reworded form2) Points that highlight the need for networking
3) Initial results of the project – results of culture and toxin analysis done by Ravi, relation between bloom and sardines done by Nashad and the bio-optical data from Shaju would be put in the poster.
4) Few points on way forward
Regarding the progress report to be presented at the NANO meeting in Australia, it was decided to include the following aspects:
1) Details of workshop2) Paper presented by Nashad on relation between HAB and sardine fishery3) Abstract at Blue Planet Symposium4) Bio-optical studies done at Kochi
Dr. Grinson documenting the parameters to be measured during SHABASHI programme
Valedictory programme was organized along with high tea at the conference hall of the CMFRI. Following a welcome address by Dr. Jayakumar, scientist at Mandapam CMFRI, the participants gave feed back on the workshop. All participants expressed satisfaction in the workshop conduct. Dr. A.K. Abdul Nazar, Scientist in Charge of Mandapam CMFRI in his valedictory speech extended all possible support to the SHABASHI programme. Distribution of certificates to the participants and memento were done by Dr. A.K. Abdul Nazar and Dr. Nandini Menon together. Dr. Nandini Menon delivered the Vote of thanks.
Model certificate issued at the end of training programme
A tour of the CMFRI Mandapam campus was arranged for the participants after the Valedictory
CMFRI Mandapam scientists explaining the Recirculating aquaculture facility at Mandapam to the participants.
The four days training programme enabled the participants to follow uniform sampling and analytical protocols for bio-optical parameters, algal pigments and toxins analysis. At the end of the workshop, the following decisions were taken regarding the sampling and analysis.
All centres would undertake sampling once in a month using the uniform protocol.
Using the collected samples, Mandapam CMFRI would do Bio-optics + HPLC analyses. Samples for phytoplankton taxonomy study would be sent to NERCI.
Vizag CMFRI would also do Bio-optics analyses. Samples for HPLC analysis would be sent to Mandapam CMFRI. Samples for phytoplankton taxonomy study would be sent to NERCI.
NERCI along with CMFRI, Kochi would do Bio-optics, phytoplankton composition, radiometer measurements and gut content analysis of sardines from samples collected off Kochi. Samples for HPLC analysis would be sent to Mandapam CMFRI.
SAC would do Bio-optics, HPLC analysis, phytoplankton composition, radiometer measurements.
Kanthi, Jayasiri and Asanthi would do Bio-optics and phytoplankton composition analyses using samples collected from SriLankan stations. Samples for HPLC analysis would be sent to Mandapam CMFRI.
Asanthi would provide details of sardine fishery in SriLanka to Grinson for comparative study.
SPATT bags would be deployed at all the stations and sent to Goa (Rajdeep Roy) for analysis.
Attempts would be done to culture the toxic algae at Mandapam CMFRI using the methodology developed by Ravidas Naik.
Common format was designed for assigning station numbers by the different centres.
Location-wise sub-files to be maintained at the centre of analysis (Mandapam, NERCI, NIO, Goa)
Cryocans to be procured for all centres using the specifications given by Amir/Gunjan.
List of parameters to be measured, centres of sampling and the responsible personsParameter Sampling location Person
responsibleParameters that would be analysed at the sampling centre
Parameters that would be analysed at a central facility
Physical parameters –TemperaturepHDOsalinity
Goa & MangaloreKochiMandapamVizagSriLankaVeraval
Ravidas NaikShajuAmir, RaviLovesonKanthiGunjan, Mini Raman
All
Chemical parameters –NitriteNitrateSilicatePhosphate
Goa & MangaloreKochiMandapamVizagSriLankaVeraval
Ravidas NaikShajuAmir, RaviLovesonKanthiGunjan, Mini Raman
All
Bio-optics – Goa & Mangalore Ravidas Naik All
Chlorophyll Total absorbanceTSMDetritusPhytoplanktonCDOM
KochiMandapamVizagSriLankaVeraval
ShajuAmir, RaviLovesonKanthiGunjan, Mini Raman
Phytoplankton -Pigment analysisTaxonomy
Goa & MangaloreKochi
Mandapam
Vizag
SriLanka
Veraval
Ravidas NaikShaju
Amir, Ravi
Loveson
Kanthi
Gunjan, Mini Raman
AllTaxonomy
Pigment
Taxonomy -Asanthi
All
Pigment –MandapamTaxonomy -KochiPigment –MandapamTaxonomy -KochiPigment –Mandapam
SPATT baganalysis for toxin
Goa & MangaloreKochiMandapamVizagSriLankaVeraval
Rajdeep RoyShajuAmir, RaviLovesonKanthiGunjan, Mini Raman
All samples to be analysed by Rajdeep Roy
Gut content analysis of sardine
Kochi Grinson, Nandini All
Radiometer data
KochiVeraval
ShajuGunjan, Mini Raman
All
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