Replikasi Virus - drh. Maxs U. E. Sanam, M.Sc. · PPT file · Web view2013-09-22 · Replikasi Virus DNA. Double-stranded DNA viruses, such as herpesviruses,papovavirusesand adenoviruses,

REPLIKASI VIRUSMax Sanam

Learning objectives

Understand stages in animal virus replication

Compare and contrast the multiplication cycle of DNA and RNA-containing animal viruses

Understand how viral proteins are synthesized

Stages of Animal Viral Replication

Attachment Entry Uncoating Biosynthesis Maturation or Assembly Release

Attachment The receptor sites of animal cells are

proteins and glycoproteins of the plasma membrane

The attachment sites of animal viruses are distributed over the surface of the virus

In many of the enveloped virus, such as influenza virus--- spikes

The receptor for a particular virus can vary from person to person

Some people lack for cellular receptor (called P antigen) for parvo virus B19

Entry Viruses enter into eukaryotic cells by

pinocytosis. An active celluar process where a cell’s plasma mebrane contiously fold inward to form vesicles

Enveloped viruses can enter by an alternative method called fusion, in which the viral envelope fuses with the plasma membrane and releases the capsid into cell’s cytoplsma (for example, HIV)

Uncoating Separation of the viral nucleic acid from

its protein coat, the capsid The capsid is digested when when the

cell attempts to digest the vesicle’s contents

Uncoating may result from the cation of lysosome enzymes of the host. These enzymes degrade the proteins of the viral capsid

The Biosynthesis Generally, DNA containing viruses

replicate their DNA in the nucleus of the host cells by using viral enzymes, and

They synthesize their capsid and other proteins in the cytoplasm by using host cell enzymes

Then the proteins migrate into the nucleus and are joined with the newly synthesized DNA to form virions.

These virions are transported along the ER to the host cell’s membrane for release

The multiplication of RNA viruses is essentially the same as that of DNA viruses, except that several different mechanisms of mRNA fromation occus among different groups of RNA viruses

RNA viruses multiply in the host cell’s cytoplasm

Maturation and Release The first step in viral maturation is the assembly of

the protein capsid The capsid may be enclosed by an envelope

consisting protein, lipid, and carbohydrate The envelope develops arround the capsid by a

process called budding Budding does not immediately killed the host cell, in

some case the host cell survive The assembled capsid containing NA pushes through

the PM. As a result, a portion of the PM, now the envelope, adheres to the virus

Nonenveloped virus are released through rupture in the host cells PM; results in the death of the host cell

Dogma Central

DNA Replication

DNA Nucleotide

O=P-O O

Phosphate Group

N Nitrogenous base (A, G, C, or T)

CH2

O

C1 C4

C3 C2

5

Sugar (deoxyribose)

Gene Expression – Transcription & Translation: Overview

Replikasi Virus DNA

Double-stranded DNA viruses, such as herpesviruses, papovaviruses and adenoviruses, which replicate in the nucleus of the cell, have a relatively direct replication strategy.

The viral DNA is transcribed by cellular DNA dependent RNA polymerase (transcriptase) to form mRNA.

In contrast, the single-stranded DNA viruses, parvoviruses and circoviruses, which also replicate in cell nuclei, utilize cellular DNA polymerase to synthesize double-stranded DNA. This is then transcribed to mRNA by cellular transcriptases.

Replikasi Virus DNA

Stages in the replication of a herpes virus, an enveloped double-stranded DNA virus.

Replikasi Virus DNA

Replication of RNA Virus Reoviruses and birnaviruses, double-

stranded RNA viruses, have segmented genomes. Transcription occurs in the cytoplasm under the direction of a viral transcriptase.

The negative-sense strand of each segment is transcribed to produce individual mRNA molecules.

In contrast, the genomes of positive-sense, single-stranded RNA viruses can act directly as mRNA after infection (Fig. 49.3).

The enzymes necessary for genome replication in these viruses are produced after infection by direct translation of virion RNA.

This RNA can bind directly to ribosomes and is translated to yield a single polyprotein which is then cleaved to yield both functional and structural proteins.

Because direct translation can occur, naked RNA extracted from such viruses is infectious.

Stages in the replication of a picornavirus, a non-enveloped, positive-sense, single-stranded RNA virus

Negative-sense single-stranded RNA viruses possess an RNA-dependent RNA polymerase.

The naked RNA of these viruses, unlike that of the positive-sense singlestranded RNA viruses, cannot initiate infection.

After infection by the virion, the genomic RNA functions as a template for transcription of positive-sense mRNA and also for virus replication, utilizing the same polymerase.

The positive-sense RNA subsequently serves as the template for synthesis of negative-sense genomic RNA.

Most single-stranded, negative-sense RNA viruses replicate in the cytoplasm of the cell.

Notable exceptions are orthomyxoviruses and Borna disease virus which replicate in the nucleus.

Stages in the replication of a rhabdovirus, an enveloped, negative-sense, single-stranded RNA virus.

Retrovirus +SS RNA The genome of retroviruses consists of positive-

sense, single-stranded RNA which does not function as messenger RNA. Instead, a single-stranded DNA copy is produced by RNA-dependent DNA polymerase (reverse transcriptase) using the viral RNA as a template.

As the second strand of DNA is formed, the parental RNA is removed from the RNA-DNA hybrid molecule. The double-stranded DNA is integrated into the host cell genome as a provirus (Fig. 49.5).

The integrated DNA provirus, which may be incorporated into cellular chromosomes at a number of sites, can be transcribed to new viral RNA.