RENIN-ANGIONTENSIN- ALDOSTERONE-SYSTEM (RAAS) BLOCKERS IN DIABETIC NEPHROPATHY (DN) Nóra Fanni Bánki SE-MTA “Lendulet” Diabetes Research Group, 1 st Dep. of Pediatrics, Academic Research Group for Pediatrics and Nephrology, Semmelweis University, Budapest 2012 V4 ACADEMIES FORUM Mátraháza, 26.10.2012 [email protected][email protected]
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RENIN-ANGIONTENSIN- ALDOSTERONE-SYSTEM (RAAS) BLOCKERS IN DIABETIC NEPHROPATHY (DN) Nóra Fanni Bánki SE-MTA “Lendulet” Diabetes Research Group, 1 st Dep.
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RENIN-ANGIONTENSIN-ALDOSTERONE-SYSTEM (RAAS)
BLOCKERS IN DIABETIC NEPHROPATHY (DN)
Nóra Fanni Bánki
SE-MTA “Lendulet” Diabetes Research Group, 1st Dep. of Pediatrics, Academic Research Group for Pediatrics and Nephrology,
Methods• After 5 weeks of STZ-induced (60 mg/kg iv.) diabetes, Wistar rats
were treated daily p.o. for 2 weeks with
a. enalapril (40 mg x kg-1 x day-1; n=6), b. losartan (20 mg x kg-1 x day-1; n=6), c. spironolactone (50 mg x kg-1 x day-1; n=6),d. epleronone (50 mg x kg-1 x day-1; n=6),e. saline (n=6).
• Blood pressure was monitored non-invasively before and after treatment with a CODA tail-cuff system.
• Serum and urine parameters were measured and histological scanning of the excised kidney was performed.
• Protein levels and intrarenal localization of Sigma-1R-Akt-NKA were evaluated.