Relatieve affiniteit voor de alfa en beta receptor
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Relatieve affiniteit voor de alfa en beta receptor
Adapted from Boterberg, 2000
E2
GENISTEIN, DAIDZEIN, AND EQUOL AND CELL-CELL ADHESION IN MCF-7/6 CELLS
DMSO Equol
Equol + ICI 182,780 Equol + MB2
SLOW AGGREGATION ASSAY
FAST AGGREGATION ASSAY
Het grote probleem variatie in resultaten van studies bij fyto oestrogenen is de grote variatie in opname van deze stoffen.
Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12th 2009
WORK PACKAGE IWORK PACKAGE I
O-desmethylangolensin Equol 8-prenylnaringenin
Daidzein Isoxanthohumol
Enterodiol (END) Enterolactone (ENL)
Secoisolariciresinol
Large interindividual variation
Daidzein
OH O
OOH
Dihydrodaidzein (DHD)
OH O
OOH
Equol
OH O
OHO-desmethylangolensin
(O-DMA)
OH O
OOH
H
SOY
Isoxanthohumol (IX)
8-Prenylnaringenin (8-PN)
OMe
OH
HOP
Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12th 2009
Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12th 2009
MIX
Daidzein
OH O
OOH
Equol
OH O
OH
IXOMe
8-PNOH ENL
SECOR: H
H3CO
HO
OR
OR
OCH3
OH
GUT MICROBIOT
A
Daidzein
OH O
OOH
Equol
OH O
OH
IXOMe
8-PNOH ENL
SECOR: H
H3CO
HO
OR
OR
OCH3
OH
GUT MICROBIOT
A
Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12th 2009
WORK PACKAGE IWORK PACKAGE I
Individual phytoestrogens Individual phytoestrogens – – SHIME runSHIME run
Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12th 2009
WORK PACKAGE IWORK PACKAGE I
Individual phytoestrogens Individual phytoestrogens – – SHIME runSHIME run DaidzeinDaidzein metabolism metabolism ((Decroos et al. (2006) J Nutr 136, 946-Decroos et al. (2006) J Nutr 136, 946-
52)52) Weak equol-producing inoculum + EPC4Weak equol-producing inoculum + EPC4 Standard SHIME feed + 175 µM daidzein aglycon eq.Standard SHIME feed + 175 µM daidzein aglycon eq.
IXIX metabolism metabolism (Possemiers et al. (2006) J Nutr 136, 1862-7; (Possemiers et al. (2006) J Nutr 136, 1862-7;
Possemiers et al. (2008) J Nutr Possemiers et al. (2008) J Nutr
138, 1310-6)138, 1310-6) Strong 8-PN-producing inoculumStrong 8-PN-producing inoculum Weak 8-PN-producing inoculum + Weak 8-PN-producing inoculum + E. limosumE. limosum Standard SHIME feed + 70 µM IX Standard SHIME feed + 70 µM IX
Stable EQUOL production in colon transversum
Stable 8-PN production in colon transversum
0
10
20
30
40
50
60
Genistein Daidzein Equol
Concentration (nm
ol/l)
Active group
Placebo group
BREAST TISSUE HOMOGENATE
Group Genistein Daidzein Equol SOY Median
Minimum Maximum N
Nd Nd Nd 9
Nd Nd
110.50 9
4.87 Nd
44.53 9
PLACEBO Median Minimum Maximum N
Nd Nd
2.79 13
5.97 Nd
30.88 13
Nd Nd
23.06 13
MATERIAL & METHODS - MATERIAL & METHODS - BREASTBREAST
FFQ, BMI, age, antibiotic use,…
bloodspot urineBreath methane
1 2 3 4 5 6 7 8 9 10R
WASHOUT TREATMENT
GC-FID
AESTHETIC BREAST SURGERY
Breast biopsies
Enzymatic deconjugationExtraction
HPLC-UV-MS
Bio-activity•biodistribution•concentration•molecular form
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